TAF250 is required for multiple developmental events in Drosophila.

Abstract:

:The TFIID transcription initiation complex is composed of TBP and multiple TAFs. Studies in unicellular systems indicate that TAF250 is required for progression through G(1)/S of the cell cycle and repression of apoptosis. Here we extend these in vivo studies by determining the developmental requirements for TAF250 in a multicellular organism, Drosophila. TAF250 mutants were isolated in a genetic screen that also yielded TAF60 and TAF110 mutants, indicating that TAFs function coordinately to regulate transcription. Null alleles of TAF250 are recessive larval lethal. However, combinations of weak loss-of-function TAF250 alleles survive to adulthood and reveal requirements for TAF250 during ovary, eye, ocelli, wing, bristle, and terminalia development as well as overall growth of the fly. These phenotypes suggest roles for TAF250 in regulating the cell cycle, cell differentiation, cell proliferation, and cell survival. Finally, molecular analysis of TAF250 mutants reveals that the observed phenotypes are caused by mutations in a central region of TAF250 that is conserved among metazoan organisms. This region is contained within the TAF250 histone acetyltransferase domain, but the mutations do not alter the histone acetyltransferase activity of TAF250 in vitro, indicating that some other aspect of TAF250 function is affected. Because this region is not conserved in the yeast TAF250 homologue, TAF145, it may define an activity for TAF250 that is unique to higher eukaryotes.

authors

Wassarman DA,Aoyagi N,Pile LA,Schlag EM

doi

10.1073/pnas.97.3.1154

keywords:

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

1154-9

issue

3

eissn

0027-8424

issn

1091-6490

journal_volume

97

pub_type

杂志文章
  • Polymerization and mechanical properties of single RecA-DNA filaments.

    abstract::The polymerization of individual RecA-DNA filaments, containing either single-stranded or double-stranded DNA, was followed in real time, and their mechanical properties were characterized with force-measuring laser tweezers. It was found that the stretch modulus of a filament is dominated by its (central) DNA compone...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.18.10109

    authors: Hegner M,Smith SB,Bustamante C

    更新日期:1999-08-31 00:00:00

  • The role of nanostructure in the wetting behavior of mixed-monolayer-protected metal nanoparticles.

    abstract::Self-assembled monolayer-protected nanoparticles are promising candidates for applications, such as sensing and drug delivery, in which the molecular ligands' interactions with the surrounding environment play a crucial role. We recently showed that, when gold nanoparticles are coated with a binary mixture of immiscib...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0803929105

    authors: Centrone A,Penzo E,Sharma M,Myerson JW,Jackson AM,Marzari N,Stellacci F

    更新日期:2008-07-22 00:00:00

  • Poly(ADP-ribose) glycohydrolase mediates oxidative and excitotoxic neuronal death.

    abstract::Excessive activation of poly(ADP-ribose) polymerase 1 (PARP1) leads to NAD(+) depletion and cell death during ischemia and other conditions that generate extensive DNA damage. When activated by DNA strand breaks, PARP1 uses NAD(+) as substrate to form ADP-ribose polymers on specific acceptor proteins. These polymers a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.211202598

    authors: Ying W,Sevigny MB,Chen Y,Swanson RA

    更新日期:2001-10-09 00:00:00

  • From likely to likable: The role of statistical typicality in human social assessment of faces.

    abstract::Humans readily form social impressions, such as attractiveness and trustworthiness, from a stranger's facial features. Understanding the provenance of these impressions has clear scientific importance and societal implications. Motivated by the efficient coding hypothesis of brain representation, as well as Claude Sha...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1912343117

    authors: Ryali CK,Goffin S,Winkielman P,Yu AJ

    更新日期:2020-11-24 00:00:00

  • Sucrose biosynthesis in a prokaryotic organism: Presence of two sucrose-phosphate synthases in Anabaena with remarkable differences compared with the plant enzymes.

    abstract::Biosynthesis of sucrose-6-P catalyzed by sucrose-phosphate synthase (SPS), and the presence of sucrose-phosphate phosphatase (SPP) leading to the formation of sucrose, have both been ascertained in a prokaryotic organism: Anabaena 7119, a filamentous heterocystic cyanobacterium. Two SPS activities (SPS-I and SPS-II) w...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.24.13600

    authors: Porchia AC,Salerno GL

    更新日期:1996-11-26 00:00:00

  • The role of immunophilins in mutant superoxide dismutase-1linked familial amyotrophic lateral sclerosis.

    abstract::It has been reported that expression of familial amyotrophic lateral sclerosis (FALS)-associated mutant Cu/Zn superoxide dismutase-1 (SOD) induces apoptosis of neuronal cells in culture associated with an increase in reactive oxygen species. SOD recently has been shown to prevent calcineurin inactivation, initiating t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.6.3251

    authors: Lee JP,Palfrey HC,Bindokas VP,Ghadge GD,Ma L,Miller RJ,Roos RP

    更新日期:1999-03-16 00:00:00

  • Chiral N-heterocyclic carbene-catalyzed generation of ester enolate equivalents from {alpha},{beta}-unsaturated aldehydes for enantioselective Diels-Alder reactions.

    abstract::The catalytic generation of chiral ester enolate equivalents from α,β-unsaturated aldehydes with chiral N-hetereocyclic carbene catalysts makes possible highly enantioselective hetero-Diels-Alder reactions. The reactions proceed under simple, mild conditions with both aliphatic and aromatic substituted enals as substr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1007469107

    authors: Kaeobamrung J,Kozlowski MC,Bode JW

    更新日期:2010-11-30 00:00:00

  • Electron transfer in the Rhodobacter sphaeroides reaction center assembled with zinc bacteriochlorophyll.

    abstract::The cofactor composition and electron-transfer kinetics of the reaction center (RC) from a magnesium chelatase (bchD) mutant of Rhodobacter sphaeroides were characterized. In this RC, the special pair (P) and accessory (B) bacteriochlorophyll (BChl) -binding sites contain Zn-BChl rather than BChl a. Spectroscopic meas...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0812719106

    authors: Lin S,Jaschke PR,Wang H,Paddock M,Tufts A,Allen JP,Rosell FI,Mauk AG,Woodbury NW,Beatty JT

    更新日期:2009-05-26 00:00:00

  • Contribution of canonical nonhomologous end joining to chromosomal rearrangements is enhanced by ATM kinase deficiency.

    abstract::A likely mechanism of chromosomal rearrangement formation involves joining the ends from two different chromosomal double-strand breaks (DSBs). These events could potentially be mediated by either of two end-joining (EJ) repair pathways [canonical nonhomologous end joining (C-NHEJ) or alternative end joining (ALT-EJ)]...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1612204114

    authors: Bhargava R,Carson CR,Lee G,Stark JM

    更新日期:2017-01-24 00:00:00

  • Evidence from UV transcription mapping in HeLa cells that heterogeneous nuclear RNA is the messenger RNA precursor.

    abstract::The effects of UV irradiation on the incorporation of [3H]uridine in HeLa (human) cell mRNA, rRNA, heterogeneous nuclear RNA (hnRNA) and early mRNA from adenovirus type 2 have been compared. The UV target size of cell mRNA is at least 3 times larger than the average size of the mRNA itself and larger than the adenovir...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.10.4520

    authors: Goldberg S,Schwartz H,Darnell JE Jr

    更新日期:1977-10-01 00:00:00

  • Platelet factor 4 is chemotactic for neutrophils and monocytes.

    abstract::Platelet factor 4 is shown to be a chemotactic protein for human polymorphonuclear leukocytes and monocytes at concentrations found in human serum and reached locally in injured tissue. The maximum chemotactic response to platelet factor 4 nearly equals that achieved with saturating concentrations of the chemotactic a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.7.4584

    authors: Deuel TF,Senior RM,Chang D,Griffin GL,Heinrikson RL,Kaiser ET

    更新日期:1981-07-01 00:00:00

  • Competition and hybridization drive interspecific territoriality in birds.

    abstract::Costly interactions between species that arise as a by-product of ancestral similarities in communication signals are expected to persist only under specific evolutionary circumstances. Territorial aggression between species, for instance, is widely assumed to persist only when extrinsic barriers prevent niche diverge...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1921380117

    authors: Drury JP,Cowen MC,Grether GF

    更新日期:2020-06-09 00:00:00

  • RAPT1, a mammalian homolog of yeast Tor, interacts with the FKBP12/rapamycin complex.

    abstract::Rapamycin is a potent immunosuppressant that blocks the G1/S transition in antigen-activated T cells and in yeast. The similar effects of rapamycin in animal cells and yeast suggest that the biochemical steps affected by rapamycin are conserved. Using a two-hybrid system we isolated mammalian clones that interact with...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.26.12574

    authors: Chiu MI,Katz H,Berlin V

    更新日期:1994-12-20 00:00:00

  • Evidence for a signaling axis by which intestinal phosphate rapidly modulates renal phosphate reabsorption.

    abstract::The mechanisms by which phosphorus homeostasis is preserved in mammals are not completely understood. We demonstrate the presence of a mechanism by which the intestine detects the presence of increased dietary phosphate and rapidly increases renal phosphate excretion. The mechanism is of physiological relevance becaus...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0704446104

    authors: Berndt T,Thomas LF,Craig TA,Sommer S,Li X,Bergstralh EJ,Kumar R

    更新日期:2007-06-26 00:00:00

  • Targeted mutation of Ncam to produce a secreted molecule results in a dominant embryonic lethality.

    abstract::The neural cell adhesion molecule (NCAM) is a membrane-associated member of the immunoglobulin superfamily capable of both homophilic and heterophilic binding. To investigate the significance of this binding, a gene targeting strategy in embryonic stem (ES) cells was used to replace the membrane-associated forms of NC...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.13.6421

    authors: Rabinowitz JE,Rutishauser U,Magnuson T

    更新日期:1996-06-25 00:00:00

  • Spatial patterns of gene expression in the olfactory bulb.

    abstract::How olfactory sensory neurons converge on spatially invariant glomeruli in the olfactory bulb is largely unknown. In one model, olfactory sensory neurons interact with spatially restricted guidance cues in the bulb that orient and guide them to their target. Identifying differentially expressed molecules in the olfact...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0404872101

    authors: Lin DM,Yang YH,Scolnick JA,Brunet LJ,Marsh H,Peng V,Okazaki Y,Hayashizaki Y,Speed TP,Ngai J

    更新日期:2004-08-24 00:00:00

  • Membrane structure and conformational changes of the antibiotic heterodimeric peptide distinctin by solid-state NMR spectroscopy.

    abstract::The heterodimeric antimicrobial peptide distinctin is composed of 2 linear peptide chains of 22- and 25-aa residues that are connected by a single intermolecular S-S bond. This heterodimer has been considered to be a unique example of a previously unrecorded class of bioactive peptides. Here the 2 distinctin chains we...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0905069106

    authors: Resende JM,Moraes CM,Munhoz VH,Aisenbrey C,Verly RM,Bertani P,Cesar A,Piló-Veloso D,Bechinger B

    更新日期:2009-09-29 00:00:00

  • Caterpillars evolved from onychophorans by hybridogenesis.

    abstract::I reject the Darwinian assumption that larvae and their adults evolved from a single common ancestor. Rather I posit that, in animals that metamorphose, the basic types of larvae originated as adults of different lineages, i.e., larvae were transferred when, through hybridization, their genomes were acquired by distan...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0908357106

    authors: Williamson DI

    更新日期:2009-11-24 00:00:00

  • Cell surface insertion of exogenous epidermal growth factor receptors into receptor- mutant cells: demonstration of insertion in the absence of added fusogenic agents.

    abstract::We show that epidermal growth factor (EGF) receptor can be transferred in a biologically active orientation from donor hepatic membranes to recipient receptorless fibroblast cells. The recipient cells (NR-6) normally lack EGF receptors and are biologically unresponsive to EGF. The transfer of receptors from donor plas...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.79.6.1893

    authors: Bishayee S,Feinman J,Pittenger M,Michael H,Das M

    更新日期:1982-03-01 00:00:00

  • Membrane vesicles from multidrug-resistant human cancer cells contain a specific 150- to 170-kDa protein detected by photoaffinity labeling.

    abstract::Multiple drug resistance of tumor cells is a common problem in cancer therapy. We have demonstrated that membrane vesicles from highly multidrug-resistant human KB carcinoma cell lines exhibit increased specific and saturable binding of vinblastine. To identify the molecules that bind vinblastine, membrane vesicles fr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.11.3847

    authors: Cornwell MM,Safa AR,Felsted RL,Gottesman MM,Pastan I

    更新日期:1986-06-01 00:00:00

  • Purification and characterization of the retina-specific cell-aggregating factor.

    abstract::The tissue-specific, cell-aggregating component of embryonic neural retina cells was purified from the retina cell-aggregating factor and characterized as a glycoprotein. Its molecular weight in solution is in the range of 50,000, and it contains 10-15% carbohydrate. The amino-acid and carbohydrate compositions have b...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.3.916

    authors: Hausman RE,Moscona AA

    更新日期:1975-03-01 00:00:00

  • Bone morphogenetic protein 2 transiently enhances expression of a gene, Id (inhibitor of differentiation), encoding a helix-loop-helix molecule in osteoblast-like cells.

    abstract::Bone morphogenetic protein 2 (BMP-2) is a potent inducer of differentiation of osteoblasts both in vivo and in vitro. We examined the action of BMP-2 on expression of a helix-loop-helix-type transcription factor, Id (inhibitor of differentiation), in osteoblast-like cells, as well as in osteoblast-enriched cells and p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.19.9219

    authors: Ogata T,Wozney JM,Benezra R,Noda M

    更新日期:1993-10-01 00:00:00

  • Fluorescence microscopy for simultaneous observation of 3D orientation and movement and its application to quantum rod-tagged myosin V.

    abstract::Single molecule fluorescence polarization techniques have been used for three-dimensional (3D) orientation measurements to observe the dynamic properties of single molecules. However, only few techniques can simultaneously measure 3D orientation and position. Furthermore, these techniques often require complex equipme...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1118472109

    authors: Ohmachi M,Komori Y,Iwane AH,Fujii F,Jin T,Yanagida T

    更新日期:2012-04-03 00:00:00

  • Fibroblast growth factor (FGF) homologous factors: new members of the FGF family implicated in nervous system development.

    abstract::Four new members of the fibroblast growth factor (FGF) family, referred to as fibroblast growth factor homologous factors (FHFs), have been identified by a combination of random cDNA sequencing, data base searches, and degenerate PCR. Pairwise comparisons between the four FHFs show between 58% and 71% amino acid seque...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.18.9850

    authors: Smallwood PM,Munoz-Sanjuan I,Tong P,Macke JP,Hendry SH,Gilbert DJ,Copeland NG,Jenkins NA,Nathans J

    更新日期:1996-09-03 00:00:00

  • Water conservation in irrigation can increase water use.

    abstract::Climate change, water supply limits, and continued population growth have intensified the search for measures to conserve water in irrigated agriculture, the world's largest water user. Policy measures that encourage adoption of water-conserving irrigation technologies are widely believed to make more water available ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0805554105

    authors: Ward FA,Pulido-Velazquez M

    更新日期:2008-11-25 00:00:00

  • L596-W733 bond between the start of the S4-S5 linker and the TRP box stabilizes the closed state of TRPV4 channel.

    abstract::Unlike other cation channels, each subunit of most transient receptor potential (TRP) channels has an additional TRP-domain helix with an invariant tryptophan immediately trailing the gate-bearing S6. Recent cryo-electron microscopy of TRP vanilloid subfamily, member 1 structures revealed that this domain is a five-tu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1502366112

    authors: Teng J,Loukin SH,Anishkin A,Kung C

    更新日期:2015-03-17 00:00:00

  • Gain-of-function mutations in complement factor B are associated with atypical hemolytic uremic syndrome.

    abstract::Hemolytic uremic syndrome (HUS) is an important cause of acute renal failure in children. Mutations in one or more genes encoding complement-regulatory proteins have been reported in approximately one-third of nondiarrheal, atypical HUS (aHUS) patients, suggesting a defect in the protection of cell surfaces against co...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0603420103

    authors: Goicoechea de Jorge E,Harris CL,Esparza-Gordillo J,Carreras L,Arranz EA,Garrido CA,López-Trascasa M,Sánchez-Corral P,Morgan BP,Rodríguez de Córdoba S

    更新日期:2007-01-02 00:00:00

  • Structure of the human retinoblastoma gene.

    abstract::Complete inactivation of the human retinoblastoma gene (RB) is believed to be an essential step in tumorigenesis of several different cancers. To provide a framework for understanding inactivation mechanisms, the structure of RB was delineated. The RB transcript is encoded in 27 exons dispersed over about 200 kilobase...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.14.5502

    authors: Hong FD,Huang HJ,To H,Young LJ,Oro A,Bookstein R,Lee EY,Lee WH

    更新日期:1989-07-01 00:00:00

  • Reconstruction of the dynamics of the climatic system from time-series data.

    abstract::The oxygen isotope record of the last million years, as provided by a deep sea core sediment, is analyzed by a method recently developed in the theory of dynamical systems. The analysis suggests that climatic variability is the manifestation of a chaotic dynamics described by an attractor of fractal dimensionality. A ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.3.536

    authors: Nicolis C,Nicolis G

    更新日期:1986-02-01 00:00:00

  • Alternative splicing of agrin regulates its binding to heparin alpha-dystroglycan, and the cell surface.

    abstract::Agrin is a basal lamina molecule that directs key events in postsynaptic differentiation, most notably the aggregation of acetylcholine receptors (AChRs) on the muscle cell surface. Agrin's AChR clustering activity is regulated by alternative mRNA splicing. Agrin splice forms having inserts at two sites (y and z) in t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.14.7369

    authors: O'Toole JJ,Deyst KA,Bowe MA,Nastuk MA,McKechnie BA,Fallon JR

    更新日期:1996-07-09 00:00:00