A novel murine oncornavirus with dual eco- and xenotropic properties.

abstract：:The molecular basis of short-day-induced growth cessation and dormancy in the meristems of perennial plants (e.g., forest trees growing in temperate and high-latitude regions) is poorly understood. Using global transcript profiling, we show distinct stage-specific alterations in auxin responsiveness of the transcripto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baba K,Karlberg A,Schmidt J,Schrader J,Hvidsten TR,Bako L,Bhalerao RP

更新日期：2011-02-22 00:00:00

abstract：:More than a thousand proteins are thought to contribute to mammalian chromatin and its regulation, but our understanding of the genomic occupancy and function of most of these proteins is limited. Here we describe an approach, which we call "chromatin proteomic profiling," to identify proteins associated with genomic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ji X,Dadon DB,Abraham BJ,Lee TI,Jaenisch R,Bradner JE,Young RA

更新日期：2015-03-24 00:00:00

abstract：:The treatment of PC12 cells with either nerve growth factor or phorbol 12-myristate 13-acetate caused a decrease in the phosphorylation of a soluble 100-kDa protein (Nsp100). After treatment with nerve growth factor, the activity of Ca2+/phospholipid-dependent protein kinase (protein kinase C) in the cytosol was incre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hama T,Huang KP,Guroff G

更新日期：1986-04-01 00:00:00

abstract：:Neurons respond to sensory stimuli by altering the rate and temporal pattern of action potentials. These spike trains both encode and propagate information that guides behavior. Local inhibitory networks can affect the information encoded and propagated by neurons by altering correlations between different spike train...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giridhar S,Doiron B,Urban NN

更新日期：2011-04-05 00:00:00

abstract：:K+ channels, which have been linked to regulation of electrogenic solute transport as well as Ca2+ influx, represent a locus in hepatocytes for the concerted actions of hormones that employ Ca2+ and cAMP as intracellular messengers. Despite considerable study, the single-channel basis for synergistic effects of Ca2+ a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lidofsky SD

更新日期：1995-07-18 00:00:00

abstract：:Temporal integration in visual perception is thought to occur within cycles of occipital alpha-band (8-12 Hz) oscillations. Successive stimuli may be integrated when they fall within the same alpha cycle and segregated for different alpha cycles. Consequently, the speed of alpha oscillations correlates with the tempor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wutz A,Melcher D,Samaha J

更新日期：2018-02-06 00:00:00

abstract：:A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit glo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aviv H,Leder P

更新日期：1972-06-01 00:00:00

abstract：:Ribosome crystal structures have revealed that two small subunit proteins, S9 and S13, have C-terminal tails, which, together with several features of 16S rRNA, contact the anticodon stem-loop of P-site tRNA. To test the functional importance of these protein tails, we created genomic deletions of the C-terminal regio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoang L,Fredrick K,Noller HF

更新日期：2004-08-24 00:00:00

abstract：:To probe the structure of the gamma heavy chain of membrane IgG and the mRNA and gene segments that encode it, we have analyzed cDNA clones derived from a gamma 1 membrane RNA of B lymphoma 2PK-3. The nucleotide sequence of the clones indicated that membrane gamma 1 chains bear a COOH-terminal 71-residue segment that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tyler BM,Cowman AF,Gerondakis SD,Adams JM,Bernard O

更新日期：1982-03-01 00:00:00

abstract：:The cytochrome P-450 superfamily of enzymes catalyzes the oxidative metabolism of innumerable lipophilic compounds (e.g., drugs, carcinogens, steroids). Although the three-dimensional structure of a soluble bacterial P-450 (P-450cam) has been solved, little is known about the structures of the membrane-bound mammalian...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kronbach T,Larabee TM,Johnson EF

更新日期：1989-11-01 00:00:00

abstract：:A simple in vitro system that supports chromatin assembly was developed for Saccharomyces cerevisiae. The assembly reaction is ATP-dependent, uses soluble histones and assembly factors, and generates physiologically spaced nucleosomes. We analyze the pathway of histone recruitment into nucleosomes, using this system i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schultz MC,Hockman DJ,Harkness TA,Garinther WI,Altheim BA

更新日期：1997-08-19 00:00:00

abstract：:Donor DNA molecules carrying Tn1 or Tn3 deletion mutants do not need to replicate in order to participate in replicon fusion recombination events during which the Tn1/Tn3 element is duplicated. We have assayed Tn1 delta Ap-mediated replicon fusion events involving plasmid R388 and the bacteriophage lambda-derived plas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Muster CJ,Shapiro JA,MacHattie LA

更新日期：1983-04-01 00:00:00

abstract：:In Caenorhabditis elegans, the small nuclear ribonucleoprotein (snRNP)-associated proteins U1A and U2B'' are approximately 50% identical to each other, and neither bears signature characteristics of mammalian U1A or U2B'' or the single Drosophila homolog, SNF. We show here that the genes that encode these proteins (rn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saldi T,Wilusz C,MacMorris M,Blumenthal T

更新日期：2007-06-05 00:00:00

abstract：:Associative memory of the mollusc Hermissenda crassicornis, previously correlated with changes of specific K+ currents, protein phosphorylation, and increased synthesis of mRNA and specific proteins, is here shown to be accompanied by macroscopic alteration in the structure of a single identified neuron, the medial ty...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alkon DL,Ikeno H,Dworkin J,McPhie DL,Olds JL,Lederhendler I,Matzel L,Schreurs BG,Kuzirian A,Collin C

更新日期：1990-02-01 00:00:00

abstract：:It has been proposed that horizontal cells of the vertebrate retina have a negative feedback synapse with cone photoreceptors. gamma-Aminobutyric acid (GABA) has been suggested to be a neurotransmitter of monophasic horizontal cells (a subtype of horizontal cells), which have direct connections with red-sensitive and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tachibana M,Kaneko A

更新日期：1984-12-01 00:00:00

abstract：:Observations with the image tube scanner at the Shane 3-m telescope are combined with data obtained with the International Ultraviolet Explorer to assess the spectrum of the remarkable high-excitation planetary nebula NGC 6537. We have analyzed the spectrum of this nitrogen-rich object with the aid of the theoretical ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feibelman W,Aller LH,Keyes CD,Czyzak SJ

更新日期：1985-04-01 00:00:00

abstract：:Insects, as a group, have been remarkably successful in adapting to a great range of physical and biological environments, in large part because of their ability to fly. The evolution of flight in small insects was accompanied by striking adaptations of the thoracic musculature that enabled very high wing beat frequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swank DM,Vishnudas VK,Maughan DW

更新日期：2006-11-14 00:00:00

abstract：:Much debate has revolved around the question of whether the mode of evolutionary and ecological turnover in the fossil record of African mammals was continuous or pulsed, and the degree to which faunal turnover tracked changes in global climate. Here, we assembled and analyzed large specimen databases of the fossil re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bibi F,Kiessling W

更新日期：2015-08-25 00:00:00

abstract：:RNA interfering is a eukaryote-specific gene silencing by 20∼23-nucleotide (nt) microRNAs and small interfering RNAs that recruit Argonaute proteins to complementary RNAs for degradation. In humans, Argonaute2 (AGO2) has been known as the only slicer while Argonaute3 (AGO3) barely cleaves RNAs. Therefore, the intrinsi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park MS,Sim G,Kehling AC,Nakanishi K

更新日期：2020-11-17 00:00:00

abstract：:Reverse transcriptase of murine retroviruses is a monomeric protein of approximately 80,000 daltons, which is encoded by the central portion of the viral pol gene. To prepare large quantities of the enzyme, we have constructed gene fusions between the trpE gene and portions of the pol gene of Moloney murine leukemia v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tanese N,Roth M,Goff SP

更新日期：1985-08-01 00:00:00

abstract：:Adrenocorticotropic hormone (ACTH) inhibited [3H]thymidine incorporation in normal adrenocortical cells of adult rats in culture, with a concomitant increase in corticosterone production and a characteristic retraction of cells. Both dibutyryl cyclic AMP and an analog of ACTH, which produces virtually no cyclic AMP, i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramachandran J,Suyama AT

更新日期：1975-01-01 00:00:00

abstract：:It was reported that the rate of elongation of partially reconstituted RNA, if prepared without centrifugation, showed dependency upon the state of protein aggregation like that found when free RNA was used as the nucleic acid source [Butler, P. J. G. (1974) J. Mol. Biol. 82, 333-341]. Butler concluded from this resul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohno T,Takahashi M,Okada Y

更新日期：1977-02-01 00:00:00

abstract：:We have investigated the pharmacologic and neurotoxic properties of 2,4,5-trihydroxyphenylalanine [topa; the 6-hydroxylated derivative of 3,4-dihydroxyphenylalanine (dopa)] in central neurons. Application of solutions of topa to the chicken eyecup preparation results in glutamatergic responses mediated predominantly b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosenberg PA,Loring R,Xie Y,Zaleskas V,Aizenman E

更新日期：1991-06-01 00:00:00

abstract：:DNA delivery of IL-12 has shown promise in reducing the toxic side effects associated with administration of recombinant human (h)IL-12 protein while maintaining the ability to inhibit tumor growth and abolish tumor metastases in animal models. We have developed a more potent version of IL-12 by using DNA shuffling an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leong SR,Chang JC,Ong R,Dawes G,Stemmer WP,Punnonen J

更新日期：2003-02-04 00:00:00

abstract：:The amphiphilic fluorescent styryl pyridinium dyes FM1-43 and FM4-64 are used to probe activity-dependent synaptic vesicle cycling in neurons. Cultured astrocytes can internalize FM1-43 and FM4-64 inside vesicles but their uptake is insensitive to the elevation of cytosolic calcium (Ca(2+)) concentration and the under...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li D,Hérault K,Oheim M,Ropert N

更新日期：2009-12-22 00:00:00

abstract：:A protein-free nucleic acid preparation method for electron microscopy is described. The basic procedure is very similar to the classical protein monolayer spreading techniques. The carrier protein (usually cytochrome c) is replaced by benzyldimethylalkylammonium chloride. Both the hypophase method and the microdiffus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vollenweider HJ,Sogo JM,Koller T

更新日期：1975-01-01 00:00:00

abstract：:Antibody specific for the native form of Chinese hamster hypoxanthine/guanine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) was used to detect the synthesis of HPRT protein in a rabbit reticulocyte lysate translation system primed with mRNA from Chinese hamster tissues and c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Melton DW,Konecki DS,Ledbetter DH,Hejtmancik JF,Caskey CT

更新日期：1981-11-01 00:00:00

abstract：:Sympatric speciation (SS), i.e., speciation within a freely breeding population or in contiguous populations, was first proposed by Darwin [Darwin C (1859) On the Origins of Species by Means of Natural Selection] and is still controversial despite theoretical support [Gavrilets S (2004) Fitness Landscapes and the Orig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li K,Hong W,Jiao H,Wang GD,Rodriguez KA,Buffenstein R,Zhao Y,Nevo E,Zhao H

更新日期：2015-09-22 00:00:00

abstract：:The highly aggressive character of melanoma makes it an excellent model for probing the mechanisms underlying metastasis, which remains one of the most difficult challenges in treating cancer. We find that miR-182, member of a miRNA cluster in a chromosomal locus (7q31-34) frequently amplified in melanoma, is commonly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Segura MF,Hanniford D,Menendez S,Reavie L,Zou X,Alvarez-Diaz S,Zakrzewski J,Blochin E,Rose A,Bogunovic D,Polsky D,Wei J,Lee P,Belitskaya-Levy I,Bhardwaj N,Osman I,Hernando E

更新日期：2009-02-10 00:00:00

abstract：:Striatal inhibition plays an important role in models of cortex-basal ganglia function and is altered in many basal ganglia diseases. The gamma-aminobutyric acid ergic spiny projection neuron comprises >95% of striatal neurons, but despite strong anatomical evidence, the electrophysiological properties and functions o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Czubayko U,Plenz D

更新日期：2002-11-26 00:00:00