Abstract:
:An ovarian cell line was developed that requires hormonal conditioning of the host for growth in vivo and that requires special factors for growth in vitro. It is necessary to prepare special culture media to demonstrate the effects of growth factors in vitro. To this end, methods were developed for removing from serum those essential factors required for the growth of ovarian cells in culture. Minimal growth occurred in medium containing fetal calf serum that had been passed through a porcelain filter. This method of depleting serum was replaced by a procedure involving passage through a carboxymethylcellulose column. Either pituitary extract or the eluate from the column restored growth in these depleted media. The eluate was more active than pituitary extract with regard to maximal growth enhancement. When the cells were incubated in the depleted media, viability, as measured by plating efficiency, decreased with incubation time. Either pituitary extract or the eluate from the column prevented such death of cells. Based on these findings, we postulate that the eluate contains both a survival factor and a growth-promoting factor for these ovarian cells, while pituitary extract contains only the survival factor.
journal_name
Proc Natl Acad Sci U S Aauthors
Nishikawa K,Armelin HA,Sato Gdoi
10.1073/pnas.72.2.483keywords:
subject
Has Abstractpub_date
1975-02-01 00:00:00pages
483-7issue
2eissn
0027-8424issn
1091-6490journal_volume
72pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2020-04-14 00:00:00
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更新日期:1998-08-04 00:00:00