Recombinant DNA molecules of bacteriophage phi chi174.

Abstract:

:Phi chi174 DNA structures containing two different parental genomes were detected genetically and examined by electron microscopy. These structures consisted of two monomeric double-stranded DNA molecules linked in a figure 8 configuration. Such DNA structures were observed to be formed preferentially in host recA+ cells or recA+ cell-free systems. Since the host recA+ allele is required for most phi chi174 recombinant formation, we conclude that the observed figure 8 molecules are intermediates in, or end products of, 1 phi chi174 recombination event. We propose that recombinant figure 8 DNA molecules arise as a result of "single-strand aggression," are stabilized by double-strand "branch migration," and represent a specific example of a common intermediate in genetic recombination.

authors

Benbow RM,Zuccarelli AJ,Sinsheimer RL

doi

10.1073/pnas.72.1.235

keywords:

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

235-9

issue

1

eissn

0027-8424

issn

1091-6490

journal_volume

72

pub_type

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