Abstract：
:In Neurospora crassa, DNA sequence duplications are detected and altered efficiently during the sexual cycle by a process known as RIP (repeat-induced point mutation). Affected sequences are subjected to multiple GC-to-AT mutations. To explore the pattern in which base changes are laid down by RIP we examined two sets of strains. First, we examined the products of a presumptive spontaneous RIP event at the mtr locus. Results of sequencing suggested that a single RIP event produces two distinct patterns of change, descended from the two strands of an affected DNA duplex. Equivalent results were obtained using an exceptional tetrad from a cross with a known duplication flanking the zeta-eta (zeta-eta) locus. The mtr sequence data were also used to further examine the basis for the differential severity of C-to-T mutations on the coding and noncoding strands in genes. The known bias of RIP toward CpA/TpG sites in conjunction with the sequence bias of Neurospora accounts for the differential effect. Finally, we used a collection of tandem repeats (from 16 to 935 bp in length) within the mtr gene to examine the length requirement for RIP. No evidence of RIP was found with duplications shorter than 400 bp while all longer tandem duplications were frequently affected. A comparison of these results with vegetative reversion data for the same duplications is consistent with the idea that reversion of long tandem duplications and RIP share a common step.
journal_name
Geneticsjournal_title
Geneticsauthors
Watters MK,Randall TA,Margolin BS,Selker EU,Stadler DRkeywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
705-14issue
2eissn
0016-6731issn
1943-2631journal_volume
153pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Spt3 of Saccharomyces cerevisiae is required for the normal transcription of many genes in vivo. Past studies have shown that Spt3 is required for both mating and sporulation, two events that initiate when cells are at G(1)/START. We now show that Spt3 is needed for two other events that begin at G(1)/START, diploid f...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-06-01 00:00:00
abstract：:Drosophila melanogaster X chromosomes were mutagenized by feeding males sucrose solutions containing ethyl methanesulfonate (EMS); the concentrations of EMS in the food were 2.5 mM, 5.0 mM, and 10.0 mM. Chromosomes were exposed to the mutagen up to three times by treating males in succeeding generations. After treatme...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-12-01 00:00:00
abstract：:Genetic drift is an important evolutionary force of strength inversely proportional to Ne , the effective population size. The impact of drift on genome diversity and evolution is known to vary among species, but quantifying this effect is a difficult task. Here we assess the magnitude of variation in drift power amon...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303622
更新日期：2020-10-01 00:00:00
abstract：:A laboratory strain of Drosophila pseudoobscura (L116) is studied that, when crossed to sex-ratio homozygous females, produces sons that exhibit varying levels of the male sex-ratio (msr) phenotype. The msr phenotype occurs only in sex-ratio males and is due to the production of a high frequency of nullo-XY sperm. The...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:Nontandem terminal chromosome duplications derived from N. crassa translocation T(I-->VI)NM103 give rise mitotically to some daughter nuclei which have become euploid by loss of one or the other of the two duplicated segments. Loss of the segment in normal sequence occurs as often as loss of the translocated segment. ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:THE FOLLOWING MODELS ARE CONSIDERED FOR THE GENETIC DETERMINATION OF QUANTITATIVE TRAITS: segregation at one locus, at two linked loci, at any number of equal and additive unlinked loci, and at one major locus and an indefinite number of equal and additive loci. In each case an appropriate likelihood is given for data...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-04-01 00:00:00
abstract：:The ade6-M26 mutation of the fission yeast Schizosaccharomyces pombe creates a meiotic recombination hotspot that elevates ade6 intragenic recombination approximately 10-15-fold. A heptanucleotide sequence including the M26 point mutation is required but not sufficient for hotspot activity. We studied the effects of p...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:The Drosophila PS1 and PS2 integrins are required to maintain the connection between the dorsal and ventral wing epithelia. If alphaPS subunits are inappropriately expressed during early pupariation, the epithelia separate, causing a wing blister. Two lines of evidence indicate that this apparent loss-of-function phen...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:The protist Physarum polycephalum is useful for analysis of several aspects of cellular and developmental biology. To expand the opportunities for experimental analysis of this organism, we have developed a method for gene replacement. We transformed Physarum amoebae with plasmid DNA carrying a mutant allele, ardD del...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:To analyze the phenotype of cells lacking the vacuolar ATPase, we inactivated the vma-1 gene, which encodes the catalytic subunit of the enzyme. Because preliminary experiments suggested the vma-1 gene was essential, we developed a method of simultaneously inactivating the gene and complementing it with a functional c...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:In multicellular organisms, genetic programs guide cells to adopt cell fates as tissues are formed during development, maintained in adults, and repaired after injury. Here we explore how a small molecule in the environment can switch a genetic program from one fate to another. Wild-type Caenorhabditis elegans XX adul...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.169409
更新日期：2014-10-01 00:00:00
abstract：:We consider a population that adapts to a gradually changing environment. Our aim is to describe how ecological and genetic factors combine to determine the genetic basis of adaptation. Specifically, we consider the evolution of a polygenic trait that is under stabilizing selection with a moving optimum. The ecologica...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.106195
更新日期：2009-12-01 00:00:00
abstract：:The Arp2/3 complex is an essential component of the actin cytoskeleton in yeast and is required for the movement of actin patches. In an attempt to identify proteins that interact with this complex in the fission yeast Schizosaccharomyces pombe, we sought high-copy suppressors of the S. pombe arp3-c1 mutant, and have ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:The contribution of mismatch repair to genetic recombination in T4 phage has been evaluated by three independent approaches: (1) testing for non-additivity of recombinant frequencies; (2) measurements of double exchange frequencies in three-factor crosses: (3) comparisons of recombination abilities of mutations occupy...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1982-12-01 00:00:00
abstract：:MCM (minichromosome maintenance) proteins function as a replication licensing factor (RLF-M), which contributes to limiting initiation of DNA replication to once per cell cycle. In the present study we show that a truncation of the pol II CTD in a S. cerevisiae strain harboring a mutation in mcm5 partially reverses it...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:We identified and characterized 14 extragenic mutations that suppressed the dominant egg-laying defect of certain lin-12 gain-of-function mutations. These suppressors defined seven genes: sup-17, lag-2, sel-4, sel-5, sel-6, sel-7 and sel-8. Mutations in six of the genes are recessive suppressors, whereas the two mutat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
abstract：:Catfish is the major aquaculture species in the United States. The hybrid catfish produced by crossing channel catfish females with blue catfish males exhibit a number of desirable production traits, but their mass production has been difficult. To introduce desirable genes from blue catfish into channel catfish throu...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-10-01 00:00:00
abstract：:There have been several attempts to estimate the average dominance (ratio of heterozygous to homozygous effects) of spontaneous deleterious mutations in Drosophila melanogaster, but these have given inconsistent results. We investigated whether transposable element (TE) insertions have higher average dominance for egg...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：:Detecting and quantifying the differences in individual genomes (i.e., genotyping), plays a fundamental role in most modern bioinformatics pipelines. Many scientists now use reduced representation next-generation sequencing (NGS) approaches for genotyping. Genotyping diploid individuals using NGS is a well-studied fie...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301468
更新日期：2018-11-01 00:00:00
abstract：:Filamentous fungi undergo cytokinesis by forming crosswalls termed septa. Here, we describe the genetic and physiological controls governing septation in Aspergillus nidulans. Germinating conidia do not form septa until the completion of their third nuclear division. The first septum is invariantly positioned at the b...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Thyroid hormone has pleiotropic effects on cochlear development, and genomic variation influences the severity of associated hearing deficits. DW/J-Pou1f1dw/dw mutant mice lack pituitary thyrotropin, which causes severe thyroid hormone deficiency and profound hearing impairment. To assess the genetic complexity of pro...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.130633
更新日期：2011-10-01 00:00:00
abstract：:DNA double-strand breaks (DSBs) are repaired by homologous recombination (HR) and nonhomologous end-joining (NHEJ). NHEJ in yeast chromosomes has been observed only when HR is blocked, as in rad52 mutants or in the absence of a homologous repair template. We detected yKu70p-dependent imprecise NHEJ at a frequency of a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-02-01 00:00:00
abstract：:Mutations in maternally transmitted symbionts can affect host fitness. In this study we investigate a mutation in an obligate bacterial symbiont (Buchnera), which has dramatic effects on the heat tolerance of pea aphid hosts (Acyrthosiphon pisum). The heat-sensitive allele arises through a single base deletion in a ho...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.117440
更新日期：2010-09-01 00:00:00
abstract：:One of the key factors contributing to the success of a quantitative trait locus (QTL) mapping experiment is the precision with which QTL positions can be estimated. We show, using simulations, that QTL mapping precision for an experimental cross can be increased by the use of a genotypically selected sample of indivi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.033746
更新日期：2005-05-01 00:00:00
abstract：:Host variation in Toll-like receptors and other innate immune signaling molecules alters infection susceptibility. However, only a portion of the variability observed in the innate immune response is accounted for by known genes in these pathways. Thus, the identification of additional genes that regulate the response...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.185314
更新日期：2016-09-01 00:00:00
abstract：:The relationship between multidimensional form of the adult mouse mandible and body size is examined from an ontogenetic perspective. The origin and ontogeny of phenotypic correlations are described in terms of genetic and environmental covariance patterns between adult skeletal morphology and growth in body weight. D...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
abstract：:The genomes of all organisms are subject to continuous bombardment of deleterious genomic mutations (DGM). Our ability to accurately estimate various parameters of DGM has profound significance in population and evolutionary genetics. The Deng-Lynch method can estimate the parameters of DGM in natural selfing and outc...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:The identification of genes affecting sociality can give insights into the maintenance and development of sociality and personality. In this study, we used the combination of an advanced intercross between wild and domestic chickens with a combined QTL and eQTL genetical genomics approach to identify genes for social ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.300810
更新日期：2018-05-01 00:00:00
abstract：:We constructed a genetic linkage map of Gibberella zeae (Fusarium graminearum) by crossing complementary nitrate-nonutilizing (nit) mutants of G. zeae strains R-5470 (from Japan) and Z-3639 (from Kansas). We selected 99 nitrate-utilizing (recombinant) progeny and analyzed them for amplified fragment length polymorphis...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-04-01 00:00:00
abstract：:A computer system for the storage and processing of microbial meiotic data has been developed. Based on the language Fortran 4, the program retrieves relevant data and determines the order, map distances, and coefficient of coincidence for any three-gene group. Meiotic data from Aspergillus nidulans were used to test ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1972-12-01 00:00:00
