Structure of cytochrome c nitrite reductase.

Abstract:

:The enzyme cytochrome c nitrite reductase catalyses the six-electron reduction of nitrite to ammonia as one of the key steps in the biological nitrogen cycle, where it participates in the anaerobic energy metabolism of dissimilatory nitrate ammonification. Here we report on the crystal structure of this enzyme from the microorganism Sulfurospirillum deleyianum, which we solved by multiwavelength anomalous dispersion methods. We propose a reaction scheme for the transformation of nitrite based on structural and spectroscopic information. Cytochrome c nitrite reductase is a functional dimer, with 10 close-packed haem groups of type c and an unusual lysine-coordinated high-spin haem at the active site. By comparing the haem arrangement of this nitrite reductase with that of other multihaem cytochromes, we have been able to identify a family of proteins in which the orientation of haem groups is conserved whereas structure and function are not.

journal_name

Nature

journal_title

Nature

authors

Einsle O,Messerschmidt A,Stach P,Bourenkov GP,Bartunik HD,Huber R,Kroneck PM

doi

10.1038/22802

keywords:

subject

Has Abstract

pub_date

1999-07-29 00:00:00

pages

476-80

issue

6743

eissn

0028-0836

issn

1476-4687

journal_volume

400

pub_type

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