Surfactant protein A mediates mycoplasmacidal activity of alveolar macrophages by production of peroxynitrite.

Abstract:

:We have previously shown that surfactant protein A (SP-A) mediates in vitro killing of mycoplasmas by alveolar macrophages (AMs) from resistant C57BL/6 mice through a nitric oxide (.NO)-dependent mechanism. Herein, SP-A-deficient [SP-A(-/-)] and inducible.NO synthase-deficient [iNOS(-/-)] mice were infected intranasally with 10(5) or 10(7) colony-forming units of Mycoplasma pulmonis. SP-A(-/-) mice were as susceptible to mycoplasmal infection as highly susceptible C3H/He mice, and far more susceptible than resistant C57BL/6 mice. iNOS(-/-) mice had significantly greater numbers of mycoplasmas and severity of lung lesions than iNOS(+/+) controls. In vitro, AMs isolated from C57BL/6 mice, activated with IFN-gamma, incubated with SP-A (25 micrograms/ml), and infected with 10(10) colony-forming units of M. pulmonis, killed mycoplasmas within 6 h. Mycoplasmal killing was abrogated by 1,000 units/ml of copper-zinc superoxide dismutase. In the absence of AMs, incubation of M. pulmonis with the peroxynitrite generator 3-morpholinosynodiomine.HCl (SIN-1) effected complete killing of mycoplasmas by 90 min in a dose-dependent manner. Addition of copper-zinc superoxide dismutase (3,000 units/ml), which converts SIN-1 to a.NO donor, prevented this killing. Neither of the reactive oxygen species generated by xanthine oxidase (10 milliunits/ml, plus 500 microM xanthine and 100 microM FeCl3), nor.NO generated by 1-propanamine-3-(2-hydroxy-2-nitroso-1-propylhydrazine (PAPA NONOate) (100 microM) killed mycoplasmas. These data establish that peroxynitrite generation by AMs is necessary for the killing of a pathogen in vitro and in vivo.

authors

Hickman-Davis J,Gibbs-Erwin J,Lindsey JR,Matalon S

doi

10.1073/pnas.96.9.4953

keywords:

subject

Has Abstract

pub_date

1999-04-27 00:00:00

pages

4953-8

issue

9

eissn

0027-8424

issn

1091-6490

journal_volume

96

pub_type

杂志文章
  • Lewis base catalysis of bromo- and iodolactonization, and cycloetherification.

    abstract::Lewis base catalyzed bromo- and iodolactonization reactions have been developed and the effects of catalyst structure on rate and cyclization selectivity have been systematically explored. The effects of substrate structure on halolactonization reactions and the interaction of those effects with the effects of catalys...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1005296107

    authors: Denmark SE,Burk MT

    更新日期:2010-11-30 00:00:00

  • Enteroendocrine precursors differentiate independently of Wnt and form serotonin expressing adenomas in response to active beta-catenin.

    abstract::Wnt signaling is required for the maintenance of intestinal stem cells and self-renewal of the intestinal epithelium. Intestinal cancers are frequently associated with mutations that activate the Wnt pathway. The role of Wnt signaling on differentiation of lineage-specific precursors in the intestine is not well chara...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0702665104

    authors: Wang Y,Giel-Moloney M,Rindi G,Leiter AB

    更新日期:2007-07-03 00:00:00

  • Sequential repression and activation of the CCAAT enhancer-binding protein-alpha (C/EBPalpha ) gene during adipogenesis.

    abstract::CCAAT enhancer-binding protein-alpha (C/EBPalpha) functions as a pleiotropic transcriptional activator of adipocyte genes during adipogenesis. Nuclear factor C/EBP undifferentiated protein (CUP), an isoform of activator protein-2alpha (AP-2alpha), binds to repressive elements in the C/EBPalpha gene promoter, silencing...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.220426097

    authors: Jiang MS,Lane MD

    更新日期:2000-11-07 00:00:00

  • Behavior-specific changes in transcriptional modules lead to distinct and predictable neurogenomic states.

    abstract::Using brain transcriptomic profiles from 853 individual honey bees exhibiting 48 distinct behavioral phenotypes in naturalistic contexts, we report that behavior-specific neurogenomic states can be inferred from the coordinated action of transcription factors (TFs) and their predicted target genes. Unsupervised hierar...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1114093108

    authors: Chandrasekaran S,Ament SA,Eddy JA,Rodriguez-Zas SL,Schatz BR,Price ND,Robinson GE

    更新日期:2011-11-01 00:00:00

  • Computing trisections of 4-manifolds.

    abstract::We describe an algorithm to compute trisections of orientable four-manifolds using arbitrary triangulations as input. This results in explicit complexity bounds for the trisection genus of a 4-manifold in terms of the number of pentachora (4-simplices) in a triangulation. ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1717173115

    authors: Bell M,Hass J,Rubinstein JH,Tillmann S

    更新日期:2018-10-23 00:00:00

  • Stochastic gene expression as a many-body problem.

    abstract::Gene expression has a stochastic component because of the single-molecule nature of the gene and the small number of copies of individual DNA-binding proteins in the cell. We show how the statistics of such systems can be mapped onto quantum many-body problems. The dynamics of a single gene switch resembles the spin-b...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2627987100

    authors: Sasai M,Wolynes PG

    更新日期:2003-03-04 00:00:00

  • Patterns of prehistoric human mobility in polynesia indicated by mtDNA from the Pacific rat.

    abstract::Human settlement of Polynesia was a major event in world prehistory. Despite the vastness of the distances covered, research suggests that prehistoric Polynesian populations maintained spheres of continuing interaction for at least some period of time in some regions. A low level of genetic variation in ancestral Poly...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.25.15145

    authors: Matisoo-Smith E,Roberts RM,Irwin GJ,Allen JS,Penny D,Lambert DM

    更新日期:1998-12-08 00:00:00

  • Science in support of the Deepwater Horizon response.

    abstract::This introduction to the Special Feature presents the context for science during the Deepwater Horizon oil spill response, summarizes how scientific knowledge was integrated across disciplines and statutory responsibilities, identifies areas where scientific information was accurate and where it was not, and considers...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1204729109

    authors: Lubchenco J,McNutt MK,Dreyfus G,Murawski SA,Kennedy DM,Anastas PT,Chu S,Hunter T

    更新日期:2012-12-11 00:00:00

  • Translocation of phospholipids from the inner to the outer membrane of Escherichia coli.

    abstract::The translocation of lipids from the inner to the outer membrane of Escherichia coli has been investigated by pulse-chase experiments. After a pulse with [2-3H]glycerol, the specific activity of the newly synthesized [3H]phosphatidylethanolamine was 5 times greater in the inner than in the outer membrane. During the c...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.4.1867

    authors: Donohue-Rolfe AM,Schaechter M

    更新日期:1980-04-01 00:00:00

  • Enhanced release of dopamine- -hydroxylase from sympathetic nerves by calcium and phenoxybenzamine and its reversal by prostaglandins.

    abstract::Dopamine-beta-hydroxylase (EC 1.14.2.1), an enzyme localized in sympathetic synaptic vesicles, was released together with norepinephrine during in vitro stimulation of the hypogastric nerve that innervates the vas deferens of the guinea pig. Stimulation of the nerve in the presence of high concentrations of calcium (7...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.68.9.2227

    authors: Johnson DG,Thoa NB,Weinshilboum R,Axelrod J,Kopin IJ

    更新日期:1971-09-01 00:00:00

  • A single mutation in poliovirus RNA-dependent RNA polymerase confers resistance to mutagenic nucleotide analogs via increased fidelity.

    abstract::Ribavirin is a nucleotide analog that can be incorporated by viral polymerases, causing mutations by allowing base mismatches. It is currently used therapeutically as an antiviral drug during hepatitis C virus infections. During the amplification of poliovirus genomic RNA or hepatitis C replicons, error frequency is k...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1232294100

    authors: Pfeiffer JK,Kirkegaard K

    更新日期:2003-06-10 00:00:00

  • Photosensitivity of a protoporphyrin-accumulating, light-sensitive mutant (visA) of Escherichia coli K-12.

    abstract::Mutations in the visA gene of Escherichia coli cause the mutant bacteria to die upon illumination with visible light. We confirmed genetically that the visA gene is a structural gene for ferrochelatase (protoheme ferro-lyase, EC 4.99.1.1). Since other mutations in the genes involved in the biosynthesis of heme can cur...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.23.10520

    authors: Nakahigashi K,Nishimura K,Miyamoto K,Inokuchi H

    更新日期:1991-12-01 00:00:00

  • A WW domain protein TAZ is a critical coactivator for TBX5, a transcription factor implicated in Holt-Oram syndrome.

    abstract::The T-box transcription factor TBX5 plays essential roles in cardiac and limb development. Various mutations in the TBX5 gene have been identified in patients with Holt-Oram syndrome, which is characterized by congenital defects in the heart and upper extremities. In this study, we identified a WW-domain-containing tr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0509109102

    authors: Murakami M,Nakagawa M,Olson EN,Nakagawa O

    更新日期:2005-12-13 00:00:00

  • Antibodies specific to the retinoic acid human nuclear receptors alpha and beta.

    abstract::Two cDNAs encoding two human receptors for retinoic acid (RA), RAR-alpha and RAR-beta, have been characterized recently. Synthetic peptides corresponding to the cDNA-deduced amino acid sequences unique to RAR-alpha and RAR-beta were used to generate anti-RAR-alpha antiserum (SP171) and anti-RAR-beta antisera (SP172 an...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.9.3089

    authors: Gaub MP,Lutz Y,Ruberte E,Petkovich M,Brand N,Chambon P

    更新日期:1989-05-01 00:00:00

  • Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly".

    abstract::The "chlamydial anomaly," first coined by James Moulder, describes the inability of researchers to detect or purify peptidoglycan (PG) from pathogenic Chlamydiae despite genetic and biochemical evidence and antibiotic susceptibility data that suggest its existence. We recently detected PG in Chlamydia trachomatis by a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1514026112

    authors: Packiam M,Weinrick B,Jacobs WR Jr,Maurelli AT

    更新日期:2015-09-15 00:00:00

  • Ordering of water molecules between phospholipid bilayers visualized by coherent anti-Stokes Raman scattering microscopy.

    abstract::We demonstrate ordered orientation of the hydration water at the surface of phospholipid bilayers by use of coherent anti-Stokes Raman scattering (CARS) microscopy, a highly sensitive vibrational imaging method recently developed. We investigated negatively charged POPS (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-ser...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1732202100

    authors: Cheng JX,Pautot S,Weitz DA,Xie XS

    更新日期:2003-08-19 00:00:00

  • S1PR1-mediated IFNAR1 degradation modulates plasmacytoid dendritic cell interferon-α autoamplification.

    abstract::Blunting immunopathology without abolishing host defense is the foundation for safe and effective modulation of infectious and autoimmune diseases. Sphingosine 1-phosphate receptor 1 (S1PR1) agonists are effective in treating infectious and multiple autoimmune pathologies; however, mechanisms underlying their clinical...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1525356113

    authors: Teijaro JR,Studer S,Leaf N,Kiosses WB,Nguyen N,Matsuki K,Negishi H,Taniguchi T,Oldstone MB,Rosen H

    更新日期:2016-02-02 00:00:00

  • Rox8 promotes microRNA-dependent yki messenger RNA decay.

    abstract::The Hippo pathway is an evolutionarily conserved regulator of organ growth and tumorigenesis. In Drosophila, oncogenic RasV12 cooperates with loss-of-cell polarity to promote Hippo pathway-dependent tumor growth. To identify additional factors that modulate this signaling, we performed a genetic screen utilizing the D...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2013449117

    authors: Guo X,Sun Y,Azad T,Janse van Rensburg HJ,Luo J,Yang S,Liu P,Lv Z,Zhan M,Lu L,Zhou Y,Ma X,Zhang X,Yang X,Xue L

    更新日期:2020-12-01 00:00:00

  • Regulation of hypothalamic malonyl-CoA by central glucose and leptin.

    abstract::Hypothalamic malonyl-CoA has been shown to function in global energy homeostasis by modulating food intake and energy expenditure. Little is known, however, about the regulation of malonyl-CoA concentration in the central nervous system. To address this issue we investigated the response of putative intermediates in t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0709778104

    authors: Wolfgang MJ,Cha SH,Sidhaye A,Chohnan S,Cline G,Shulman GI,Lane MD

    更新日期:2007-12-04 00:00:00

  • Genomic clone for sandbar shark lambda light chain: generation of diversity in the absence of gene rearrangement.

    abstract::While the general structure of immunoglobulin chains has remained relatively unchanged throughout evolution, the organization of the genes encoding these molecules differs substantially. To understand how the rearranging immunoglobulin system arose, it is necessary to examine living representatives of the most early v...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.21.9882

    authors: Hohman VS,Schuchman DB,Schluter SF,Marchalonis JJ

    更新日期:1993-11-01 00:00:00

  • Protein kinase CK2 governs the molecular decision between encephalitogenic TH17 cell and Treg cell development.

    abstract::T helper 17 (TH17) cells represent a discrete TH cell subset instrumental in the immune response to extracellular bacteria and fungi. However, TH17 cells are considered to be detrimentally involved in autoimmune diseases like multiple sclerosis (MS). In contrast to TH17 cells, regulatory T (Treg) cells were shown to b...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1523869113

    authors: Ulges A,Witsch EJ,Pramanik G,Klein M,Birkner K,Bühler U,Wasser B,Luessi F,Stergiou N,Dietzen S,Brühl TJ,Bohn T,Bündgen G,Kunz H,Waisman A,Schild H,Schmitt E,Zipp F,Bopp T

    更新日期:2016-09-06 00:00:00

  • Single-cell imaging of retinal ganglion cell apoptosis with a cell-penetrating, activatable peptide probe in an in vivo glaucoma model.

    abstract::Molecular imaging probes have potential for in vivo identification of apoptosis and other intracellular processes. TcapQ, a cell-penetrating, near-infrared fluorescent peptide probe designed to be optically silent through intramolecular fluorescence quenching and activated by effector caspases, has been previously des...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0812884106

    authors: Barnett EM,Zhang X,Maxwell D,Chang Q,Piwnica-Worms D

    更新日期:2009-06-09 00:00:00

  • Self-organization into ferroelectric and antiferroelectric crystals via the interplay between particle shape and dipolar interaction.

    abstract::Ferroelectricity and antiferroelectricity are widely seen in various types of condensed matter and are of technological significance not only due to their electrical switchability but also due to intriguing cross-coupling effects such as electro-mechanical and electro-caloric effects. The control of the two types of d...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1809004115

    authors: Takae K,Tanaka H

    更新日期:2018-10-02 00:00:00

  • Proteomic analysis of skeletal organic matrix from the stony coral Stylophora pistillata.

    abstract::It has long been recognized that a suite of proteins exists in coral skeletons that is critical for the oriented precipitation of calcium carbonate crystals, yet these proteins remain poorly characterized. Using liquid chromatography-tandem mass spectrometry analysis of proteins extracted from the cell-free skeleton o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1301419110

    authors: Drake JL,Mass T,Haramaty L,Zelzion E,Bhattacharya D,Falkowski PG

    更新日期:2013-03-05 00:00:00

  • Neurogenesis in dentate subgranular zone and rostral subventricular zone after focal cerebral ischemia in the rat.

    abstract::Because neurogenesis persists in the adult mammalian brain and can be regulated by physiological and pathological events, we investigated its possible involvement in the brain's response to focal cerebral ischemia. Ischemia was induced by occlusion of the middle cerebral artery in the rat for 90 min, and proliferating...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.081011098

    authors: Jin K,Minami M,Lan JQ,Mao XO,Batteur S,Simon RP,Greenberg DA

    更新日期:2001-04-10 00:00:00

  • Epstein-Barr virus EBNA2 blocks Nur77- mediated apoptosis.

    abstract::Epstein-Barr virus infection in vitro immortalizes primary B cells. EBNA2 is an Epstein-Barr virus-encoded transcriptional transactivator that mimics the effects of activated Notch signaling and is essential for this proliferative response. An assay using Sindbis virus (SV) as a cell death inducer revealed that, like ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.182552499

    authors: Lee JM,Lee KH,Weidner M,Osborne BA,Hayward SD

    更新日期:2002-09-03 00:00:00

  • Ultrahigh-throughput screening in drop-based microfluidics for directed evolution.

    abstract::The explosive growth in our knowledge of genomes, proteomes, and metabolomes is driving ever-increasing fundamental understanding of the biochemistry of life, enabling qualitatively new studies of complex biological systems and their evolution. This knowledge also drives modern biotechnologies, such as molecular engin...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0910781107

    authors: Agresti JJ,Antipov E,Abate AR,Ahn K,Rowat AC,Baret JC,Marquez M,Klibanov AM,Griffiths AD,Weitz DA

    更新日期:2010-03-02 00:00:00

  • Dynamic integration of information about salience and value for saccadic eye movements.

    abstract::Humans shift their gaze to a new location several times per second. It is still unclear what determines where they look next. Fixation behavior is influenced by the low-level salience of the visual stimulus, such as luminance, contrast, and color, but also by high-level task demands and prior knowledge. Under natural ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1115638109

    authors: Schütz AC,Trommershäuser J,Gegenfurtner KR

    更新日期:2012-05-08 00:00:00

  • Adverse childhood experiences and physiological wear-and-tear in midlife: Findings from the 1958 British birth cohort.

    abstract::Allostatic load (AL) is a measure of overall physiological wear-and-tear over the life course, which could partially be the consequence of early life exposures. AL could allow a better understanding of the potential biological pathways playing a role in the construction of the social gradient in adult health. To explo...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1417325112

    authors: Barboza Solís C,Kelly-Irving M,Fantin R,Darnaudéry M,Torrisani J,Lang T,Delpierre C

    更新日期:2015-02-17 00:00:00

  • Production of active bovine tracheal antimicrobial peptide in milk of transgenic mice.

    abstract::Tracheal antimicrobial peptide (TAP) is a member of the beta-defensin family of antibiotic peptides found in the tracheal mucosa of the cow. TAP gene expression in the bovine airway is inducible by lipopolysaccharide and inflammatory mediators, suggesting that it functions to protect the upper airway from infection. L...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.24.14118

    authors: Yarus S,Rosen JM,Cole AM,Diamond G

    更新日期:1996-11-26 00:00:00