Retrieval, maturation, and fertilization of immature oocytes obtained from unstimulated patients with polycystic ovary syndrome.

Abstract:

PURPOSE:Our purpose was to determine whether immature oocytes could be retrieved under local anesthesia, whether these oocytes would mature and fertilize in vitro, and whether adequate endometrium development could be obtained after hormonal supplementation. METHODS:Ovum pick-up was performed under local anesthesia. Immature oocytes were cultured and inseminated. To prepare the endometrium, estradiolvalerate was administered in combination with micronized progesterone. RESULTS:Immature oocytes were obtained in all cases. Fifty-six percent (n = 30) of the oocytes developed into metaphase II (MII) after 48 hr of culture, and another 20% reached the MII stage by 72 hr. Normal fertilization was observed in only 10% of oocytes inseminated. No embryonic development occurred, and therefore embryo transfer was not performed in any of the patients. Endometrial microbiopsy was performed in all subjects and endometrial development was considered sufficient in eight patients. CONCLUSIONS:We collected immature oocytes from patients with polycystic ovary syndrome without general anesthesia. In vitro maturation of these oocytes seemed adequate but fertilization rates were poor. Sufficient endometrial quality was obtained after hormonal substitution.

journal_name

J Assist Reprod Genet

authors

Beckers NG,Pieters MH,Ramos L,Zeilmaker GH,Fauser BC,Braat DD

doi

10.1023/a:1022516806423

keywords:

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

81-6

issue

2

eissn

1058-0468

issn

1573-7330

journal_volume

16

pub_type

临床试验,杂志文章
  • Author Correction: A novel oocyte maturation trigger using 1500 IU of human chorionic gonadotropin plus 450 IU of follicle-stimulating hormone may decrease ovarian hyperstimulation syndrome across all in vitro fertilization stimulation protocols.

    abstract::The original version of this article unfortunately contained a mistake. The middle initial of Douglas A. Mata was omitted. The original article has been corrected. ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,已发布勘误

    doi:10.1007/s10815-017-1093-1

    authors: Anaya Y,Mata DA,Letourneau J,Cakmak H,Cedars MI,Rosen MP

    更新日期:2018-02-01 00:00:00

  • Vitamin E effect on controlled ovarian stimulation of unexplained infertile women.

    abstract:PURPOSE:To determine Vit E effect on the treatment outcomes of women with unexplained infertility undergoing controlled ovarian stimulation and intrauterine insemination (IUI). METHODS:The study group (Group A, n053) underwent controlled ovarian stimulation with clomiphene citrate with Vit E administration, 400 IU/day...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s10815-012-9714-1

    authors: Cicek N,Eryilmaz OG,Sarikaya E,Gulerman C,Genc Y

    更新日期:2012-04-01 00:00:00

  • Disparities in reproductive outcomes according to the endometrial preparation protocol in frozen embryo transfer : The risk of early pregnancy loss in frozen embryo transfer cycles.

    abstract:PURPOSE:The purpose of this study was to determine the effect of stimulated and artificial endometrial preparation protocols on reproductive outcomes in frozen embryo transfer (FET) cycles. METHODS:We performed a retrospective study of 1926 FET cycles over a 3.5-year period in the Fertility Unit at a University Hospit...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-017-1078-0

    authors: Hatoum I,Bellon L,Swierkowski N,Ouazana M,Bouba S,Fathallah K,Paillusson B,Bailly M,Boitrelle F,Alter L,Bergère M,Selva J,Wainer R

    更新日期:2018-03-01 00:00:00

  • Can apoptosis and necrosis coexist in ejaculated human spermatozoa during in vitro semen bacterial infection?

    abstract:PURPOSE:To evaluate whether ejaculated human spermatozoa undergo complete apoptosis or necrosis during experimental semen bacterial infection in vitro. METHODS:Apoptotic markers, including mitochondrial transmembrane potential (ΔΨm), phosphatidylserine (PS) externalization, and DNA fragmentation, have been detected si...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-015-0462-x

    authors: Fraczek M,Hryhorowicz M,Gaczarzewicz D,Szumala-Kakol A,Kolanowski TJ,Beutin L,Kurpisz M

    更新日期:2015-05-01 00:00:00

  • Altered bivalent positioning in metaphase I human spermatocytes from Robertsonian translocation carriers.

    abstract:PURPOSE:The study aims to determine whether there is an altered bivalent positioning in metaphase I human spermatocytes from Robertsonian translocation carriers. METHODS:Metaphase I human spermatocytes from three 45,XY,der(13;14)(q10;q10) individuals and a 45,XY,der(14;15)(q10;q10) individual were analyzed. Proximity ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-016-0809-y

    authors: Solé M,Blanco J,Valero O,Vergés L,Vidal F,Sarrate Z

    更新日期:2017-01-01 00:00:00

  • Results from the survey for preservation of adolescent reproduction (SPARE) study: gender disparity in delivery of fertility preservation message to adolescents with cancer.

    abstract:PURPOSE:Diminished reproductive capacity is a devastating consequence of life-sparing therapies for childhood malignancy. In 2006, the American Society of Clinical Oncology (ASCO) published fertility preservation recommendations (ASCOR) emphasizing the importance of early discussion and intervention for fertility prese...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-010-9504-6

    authors: Köhler TS,Kondapalli LA,Shah A,Chan S,Woodruff TK,Brannigan RE

    更新日期:2011-03-01 00:00:00

  • Clinical diagnostic testing for the cytogenetic and molecular causes of male infertility: the Mayo Clinic experience.

    abstract:PURPOSE:Approximately 8% of couples attempting to conceive are infertile and male infertility accounts for approximately 50% of infertility among couples. Up to 25% of males with non-obstructive infertility have chromosomal abnormalities and/or microdeletions of the long arm of the Y-chromosome. These are detected by c...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,meta分析

    doi:10.1007/s10815-011-9633-6

    authors: Hofherr SE,Wiktor AE,Kipp BR,Dawson DB,Van Dyke DL

    更新日期:2011-11-01 00:00:00

  • Delivery of a chromosomally normal child from an oocyte with reciprocal aneuploid polar bodies.

    abstract:PURPOSE:To demonstrate that a euploid embryo derived from an oocyte with reciprocal aneuploid polar bodies is capable of producing a chromosomally normal child. METHODS:A case report of maternal MI error compensation where single nucleotide polymorphism (SNP) microarray based comprehensive chromosome screening (CCS) w...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-012-9746-6

    authors: Scott RT Jr,Treff NR,Stevens J,Forman EJ,Hong KH,Katz-Jaffe MG,Schoolcraft WB

    更新日期:2012-06-01 00:00:00

  • Neonatal outcome of early rescue ICSI and ICSI with ejaculated sperm.

    abstract:PURPOSE:To evaluate the impact of early rescue ICSI on neonatal outcome. METHODS:This retrospective study compared the neonatal outcome of early rescue ICSI and ICSI with ejaculated sperm, including 233 children who were conceived after early rescue ICSI and a control group of 906 children who were conceived after ICS...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-014-0245-9

    authors: Chen L,Xu Z,Zhang N,Wang B,Chen H,Wang S,Sun H

    更新日期:2014-07-01 00:00:00

  • Not many shoulders of giants to stand on these days.

    abstract:: ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 社论

    doi:10.1007/s10815-018-1201-x

    authors: Albertini DF

    更新日期:2018-04-01 00:00:00

  • Editing the human genome: where ART and science intersect.

    abstract::The rapid development of gene-editing technologies has led to an exponential rise in both basic and translational research initiatives studying molecular processes and investigating possible clinical applications. Early experiments using genome editing to study human embryo development have contradicted findings in st...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 社论

    doi:10.1007/s10815-018-1219-0

    authors: Hershlag A,Bristow SL

    更新日期:2018-08-01 00:00:00

  • MicroRNAs association with azoospermia, oligospermia, asthenozoospermia, and teratozoospermia: a systematic review.

    abstract::Infertility is a major health problem across the world. One of the main reasons for male infertility are defects in sperm. Semen analysis is the most common test utilized to evaluate male fertility and since it suffers from multiple drawbacks, reproduction scientists have tried to find new molecular markers for detect...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-019-01674-9

    authors: Daneshmandpour Y,Bahmanpour Z,Hamzeiy H,Mazaheri Moghaddam M,Mazaheri Moghaddam M,Khademi B,Sakhinia E

    更新日期:2020-04-01 00:00:00

  • Association between miR-146a C > G, miR-149 T > C, miR-196a2 T > C, and miR-499 A > G polymorphisms and susceptibility to idiopathic recurrent pregnancy loss.

    abstract:BACKGROUND:A growing body of evidence suggests that microRNAs play fundamental regulatory roles in embryo implantation and maintenance of pregnancy. The aim of this study was to investigate the possible association between miR-146a C > G, miR-149 T > C, miR-196a2 T > C, and miR-499 A > G polymorphisms and genetic susce...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-019-01573-z

    authors: Alipour M,Abtin M,Hosseinzadeh A,Maleki M

    更新日期:2019-11-01 00:00:00

  • Chromosomal abnormalities in couples with recurrent spontaneous miscarriage: a 21-year retrospective study, a report of a novel insertion, and a literature review.

    abstract:PURPOSE:The aim of this study is to evaluate the frequency and nature of chromosomal abnormalities in Moroccan couples with recurrent spontaneous miscarriage (RSM). In addition, the data were compared with those reported elsewhere in order to give a global estimation of chromosomal abnormalities frequencies. METHODS:T...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-018-1373-4

    authors: Elkarhat Z,Kindil Z,Zarouf L,Razoki L,Aboulfaraj J,Elbakay C,Nassereddine S,Nasser B,Barakat A,Rouba H

    更新日期:2019-03-01 00:00:00

  • Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy.

    abstract:PURPOSE:The aim of this study was to analyze the seminal plasma of patients with idiopathic/male factor infertility and healthy controls with proven fertility by NMR spectroscopy, with a hope of establishing difference in biomarker profiles, if any, between the groups. METHODS:A total of 103 subjects visiting the infe...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-014-0282-4

    authors: Jayaraman V,Ghosh S,Sengupta A,Srivastava S,Sonawat HM,Narayan PK

    更新日期:2014-09-01 00:00:00

  • Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study.

    abstract:PURPOSE:To assess the effect of assisted hatching (AH) site on the clinical outcomes in vitrified-warmed blastocyst transfer cycles. METHODS:A total of 160 women who underwent vitrified-warmed blastocyst transfer cycles were randomized to either the ICM group (AH performing at the site near the inner cell mess, ICM), ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s10815-013-9984-2

    authors: Ren X,Liu Q,Chen W,Zhu G,Zhang H

    更新日期:2013-06-01 00:00:00

  • Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles.

    abstract:PURPOSE:The purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles. METHODS:We collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-016-0648-x

    authors: Kim K,Bloom MS,Fujimoto VY,Bell EM,Yucel RM,Browne RW

    更新日期:2016-03-01 00:00:00

  • Relationship between cytokines and the embryotoxicity of hydrosalpingeal fluid.

    abstract:OBJECTIVE:The exact chemical composition of hydrosalpingeal fluid is unknown. The objective of this study was to characterize cytokines in hydrosalpingeal fluid (HSF) and examine their possible role in the embryo development. STUDY DESIGN:HSF was aspirated at laparoscopic salpingectomy in eight infertile women. Levels...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-005-4913-7

    authors: Bedaiwy MA,Falcone T,Goldberg JM,Attaran M,Sharma R,Miller K,Nelson DR,Agarwal A

    更新日期:2005-04-01 00:00:00

  • The prevalence of ureaplasma urealyticum, mycoplasma hominis, chlamydia trachomatis and neisseria gonorrhoeae infections, and the rubella status of patients undergoing an initial infertility evaluation.

    abstract:PURPOSE:To determine the prevalence of positive test for Ureaplasma urealyticum (UU), Mycoplasma hominis (MH), Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) infections, and their corresponding Rubella status when undergoing workup for infertility. METHODS:Retrospective chart review to determine infection stat...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-007-9192-z

    authors: Imudia AN,Detti L,Puscheck EE,Yelian FD,Diamond MP

    更新日期:2008-01-01 00:00:00

  • Aging, hyaluronidase removal of the cumulus, and microinjection do not affect the sperm binding potential of human oocytes.

    abstract:PURPOSE:We studied the influence of aging, hyaluronidase removal of the cumulus, and microinjection on the sperm binding potential of human oocytes under intact zona assay conditions to determine the safe use of unstored aged unfertilized ICSI oocytes on zona binding tests. Results were also compared with those for age...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/BF02765778

    authors: Barros A,Sousa M,Silva J,Almeida V,Rocha E

    更新日期:1997-02-01 00:00:00

  • Sperm chromatin condensation defects, but neither DNA fragmentation nor aneuploidy, are an independent predictor of clinical pregnancy after intracytoplasmic sperm injection.

    abstract:PURPOSE:The impact of sperm DNA damage on intracytoplasmic sperm injection (ICSI) outcomes remains controversial. The purpose of the study was to evaluate the prognostic value of several types of sperm nuclear damage on ICSI clinical pregnancy. METHODS:Our retrospective study included a total of 132 couples who consul...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-019-01471-4

    authors: Bichara C,Berby B,Rives A,Jumeau F,Letailleur M,Setif V,Sibert L,Rondanino C,Rives N

    更新日期:2019-07-01 00:00:00

  • A novel male 2;4;14 complex chromosomal translocation with normal semen parameters but 100% embryonic aneuploidy.

    abstract::We report a case of a couple with a history of six spontaneous miscarriages in which a novel complex chromosomal rearrangement was detected in the male partner who had a totally normal semen analysis. Preimplantation genetic testing of their embryos demonstrated 100% aneuploidy. ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-018-1126-4

    authors: Mas J,Sabouni R,Bocca S

    更新日期:2018-05-01 00:00:00

  • The impact of pronuclear orientation to select chromosomally normal embryos.

    abstract:PURPOSE:To find an effective method to select embryos from ICSI with better chromosomal status when preimplantation-genetic-diagnosis (PGD) is not applied. METHODS:Several morpholological evaluations were done in same embryos. Embryos from ICSI were classified on day 1 according to pronuclear-nucleoli arrangement. On ...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-005-4874-x

    authors: Borges EJ,Rossi LM,Farah L,Guilherme P,Rocha CC,Ortiz V,Iaconelli AJ

    更新日期:2005-03-01 00:00:00

  • Experience with subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) on unfertilized aged human oocytes.

    abstract:OBJECTIVE:The aim of this study was to assess the fertilizability of unfertilized aged human oocytes from failed in vitro fertilization (IVF) cycles using SUZI and ICSI. METHODS:A total of 363 oocytes which showed no fertilization after conventional IVF was subjected to assisted fertilization using SUZI or ICSI. The m...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 临床试验,杂志文章

    doi:10.1007/BF02211724

    authors: Tsirigotis M,Bennett V,Nicholson N,Khalifa Y,Hogewind G,Yazdani N,Craft I

    更新日期:1994-09-01 00:00:00

  • Recent trends in embryo disposition choices made by patients following in vitro fertilization.

    abstract:PURPOSE:To assess longitudinal trends in in vitro fertilization (IVF) patients' choices for disposing of cryopreserved embryos. METHODS:This is a retrospective cohort study of embryo disposition forms submitted between January 2000 and February 2020 at a university-based fertility clinic. Primary outcome was dispositi...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-020-01927-y

    authors: Alexander VM,Riley JK,Jungheim ES

    更新日期:2020-11-01 00:00:00

  • The influences of weather on patients with different ovarian responses in the treatment of assisted reproductive technology.

    abstract:PURPOSE:The purpose of this study was to evaluate the influences of tropical weather on patients with different ovarian responses in the treatment of assisted reproductive technology. METHODS:Six-hundred fourty-seven women underwent their first treatment cycles were retrospectively analyzed. Patients received embryo t...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-005-4922-6

    authors: Chang SY,Lan KC,Chen CW,Huang FJ,Tsai MY,Chang CY

    更新日期:2005-05-01 00:00:00

  • Lack of association of INS VNTR polymorphism with polycystic ovary syndrome: a meta-analysis.

    abstract:PURPOSE:An association between the INS VNTR polymorphisms and polycystic ovary syndrome (PCOS) susceptibility has been reported in previous studies, but the results were inconsistent. This study was conducted to explore this association using meta-analysis. METHODS:PubMed, Embase, and China National Knowledge Infrastr...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章,meta分析

    doi:10.1007/s10815-014-0211-6

    authors: Song LY,Luo JR,Peng QL,Wang J,Xie L,He Y,Li S,Qin X

    更新日期:2014-06-01 00:00:00

  • Novel variants in women with premature ovarian function decline identified via whole-exome sequencing.

    abstract:PURPOSE:To investigate the potential etiologies of premature ovarian insufficiency (POI) and diminished ovarian reserve (DOR). METHODS:Fourteen women with sporadic POI and 6 women with DOR were enrolled. We used whole-exome sequencing (WES) and bioinformatics analysis to identify variants in a subset of 599 selected P...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-020-01919-y

    authors: Tang R,Yu Q

    更新日期:2020-10-01 00:00:00

  • Obstetric outcome and incidence of congenital anomalies in 2351 IVF/ICSI babies.

    abstract:PURPOSE:The aim of this study was to provide a comprehensive follow-up of fetal and perinatal outcome and the incidence of congenital anomalies in babies born after fresh embryo transfers compared to those conceived spontaneously in infertile couples. METHODS:Retrospective comparative analysis of all clinical pregnanc...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 杂志文章

    doi:10.1007/s10815-016-0714-4

    authors: Levi Setti PE,Moioli M,Smeraldi A,Cesaratto E,Menduni F,Livio S,Morenghi E,Patrizio P

    更新日期:2016-06-01 00:00:00

  • The ups and downs of somatic cell nucleus transfer (SCNT) in humans.

    abstract::Achieving successful somatic cell nuclear transfer (SCNT) in the human and subhuman primate relative to other mammals has been questioned for a variety of technical and logistical issues. Here we summarize the gradual evolution of SCNT technology from the perspective of oocyte quality and cell cycle status that has re...

    journal_title:Journal of assisted reproduction and genetics

    pub_type: 历史文章,杂志文章,评审

    doi:10.1007/s10815-013-0053-7

    authors: Fulka J Jr,Langerova A,Loi P,Ptak G,Albertini D,Fulka H

    更新日期:2013-08-01 00:00:00