Abstract:
:Endothelial-neutrophil adhesion is a critical step in acute inflammatory diseases, which is mediated in part by P-selectin and platelet-activating factor (PAF). Nitric oxide (NO) is well known as an endogenous second messenger derived from endothelial cells, and regulates many important physiological events, however, the direct effects of NO on endothelial-neutrophil adhesion is less well understood. The objective of this study was to examine whether, and how relatively high levels of exogenous NO increases neutrophil adhesion with respect to P-selectin and PAF. Endothelial monolayers were exposed to chemical agents for 30 min, and the adhesion of 51Cr-labeled neutrophils measured in a static adhesion assay. Spermine-NONOate (SNO), an NO donor, significantly increased neutrophil adhesion and expression of P-selectin at a concentration of 1 mM. SNO (1 mM)-mediated neutrophil adhesion was significantly inhibited by a protein kinase G inhibitor, KT5823 (0.5 microM), but not by a classical protein kinase C inhibitor, Gö6976 (10 nM), a tyrosine kinase inhibitor, genistein (1 microM), or a protein kinase A inhibitor, H-89 (0.1 microM). P-selectin surface expression induced by 1 mM SNO was also significantly inhibited by 0.5 microM KT5823. Conversely, a cytoplasm calcium chelator, TMB-8 (0.1 mM), significantly exacerbated both the neutrophil adhesion and P-selectin expression induced by SNO. WEB 2086 (10 microM), a PAF receptor antagonist, blocked neutrophil adhesion, but did not block P-selectin expression induced by SNO. These data suggest that NO increases endothelial-neutrophil adhesion through protein kinase G-mediated P-selectin mobilization to the cell surface and endothelial PAF synthesis.
journal_name
Inflammationjournal_title
Inflammationauthors
Okayama N,Coe L,Itoh M,Alexander JSdoi
10.1023/a:1020287516911keywords:
subject
Has Abstractpub_date
1999-02-01 00:00:00pages
37-50issue
1eissn
0360-3997issn
1573-2576journal_volume
23pub_type
杂志文章相关文献
INFLAMMATION文献大全abstract::Platelets activated with physiological agonists, such as thrombin, ADP, or collagen, released products able to modulate neutrophil functions. In particular, platelet supernatant contained an inhibitor of superoxide anion generation induced by phorbol ester and a chemotactic factor for human neutrophils. The proteolyti...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF00918954
更新日期:1992-04-01 00:00:00
abstract::The effects were studied of the non-specific phosphodiesterase inhibitor, theophylline (37.5-300 microM), on intracellular levels of cyclic adenosine monophosphate (cAMP) and superoxide generation following exposure of human neutrophils to four different stimuli of neutrophil membrane-associated oxidative metabolism, ...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1023/a:1022306328960
更新日期:1998-12-01 00:00:00
abstract::Gossypol is present in antiinflammatory poultices made from the medicinal tree Thespesia populnea. Isolated human neutrophils exposed to 3-20 microM gossypol for 15-90 min were assayed in vitro for superoxide production and surface expression of Mac-1 (CD11b/CD18). Gossypol increased superoxide production in a time- a...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF01560692
更新日期:1994-10-01 00:00:00
abstract::1,8-Cineol is a major monoterpene principally from eucalyptus essential oils and has been shown to exert anti-inflammatory, antiviral, and inhibitory of nuclear factor (NF)-kB effect. In the present study, we evaluated the effect of 1,8-cineol on mice infected with influenza A virus. We found that 1,8-cineol protects ...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-016-0394-3
更新日期:2016-08-01 00:00:00
abstract::Synovium-derived mesenchymal stem cells (SMSCs) can migrate to the site of destroyed condylar cartilage and differentiate into chondrocytes to repair temporomandibular joint (TMJ) damage. Interleukin (IL)-1β-induced IL-6 secretion has been shown to inhibit the chondrogenic potential of SMSCs. The histone deacetylase i...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-020-01204-1
更新日期:2020-08-01 00:00:00
abstract::TanshinoneIIA (TanIIA) has been demonstrated to possess numerous biological effects. However, the specific effect of TanIIA on macrophage polarization has not been reported. In this study, it was revealed that TanIIA might play a pivotal role in macrophage polarization. As our results indicated, cell morphology was ch...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-018-0891-7
更新日期:2019-02-01 00:00:00
abstract::This study investigated the expression of miRNA-221 in asthmatics in order to determine whether miRNA-221 plays a role in the development of asthma. Real-time PCR was used to detect the miRNA-221 in both asthmatic and control subjects. In addition, airway inflammation was evaluated by cell counting and tissue biopsy i...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-012-9474-1
更新日期:2012-08-01 00:00:00
abstract::Myeloperoxidase (MPO), next to the NO synthase2 (NOS2), and NADPH oxidase, is the key enzyme of the oxidative burst responsible for the antimicrobial immunity. Because MPO participates in the eradication of Mycobacterium tuberculosis in the in vitro model and the extracellular enzyme may activate cells to cytokine syn...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1023/b:ifla.0000033020.28446.a6
更新日期:2004-04-01 00:00:00
abstract::C-reactive protein (CRP) is a much used biomarker for respiratory tract infection; however, the influence of airway infection on the CRP level in the general population has not been well described. The study aimed to evaluate the impact of recent symptoms of airway infection on the CRP level and how the predictive pow...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-011-9405-6
更新日期:2012-06-01 00:00:00
abstract::In vivo effects of cytokine-induced neutrophil chemotactic factor (CINC) derived from rats on neutrophil infiltration were investigated using an air-pouch-type inflammation model in rats, and effects of dexamethasone on neutrophil infiltration induced by CINC was also examined in order to gain further insight into the...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF00918958
更新日期:1992-04-01 00:00:00
abstract::This experiment was conducted to investigate the effects of a benzylideneacetophenone derivative ((2E)-3-(4-hydroxy-3-methoxyphenyl)phenylpro-2-en-l-one (JC3)) on trimellitic anhydride (TMA)-induced atopic dermatitis (AD)-like symptoms in mice. To induce AD, the dorsal skins of mice were treated with 5% TMA on day 0 a...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-019-00971-w
更新日期:2019-06-01 00:00:00
abstract::Air pollution events frequently occur in China during the winter. Most investigations of pollution studies have focused on the physical and chemical properties of PM2.5. Many of these studies have indicated that PM2.5 exacerbates asthma or eosinophil inflammation. However, few studies have evaluated the relationship b...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-019-01071-5
更新日期:2020-02-01 00:00:00
abstract::Dysregulated release of neutrophil reactive oxygen species and proteolytic enzymes contributes to both acute and chronic inflammatory diseases. Therefore, molecular regulators of these processes are potential targets for new anti-inflammatory therapies. We have shown previously that myristoylated alanine-rich C-kinase...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-014-0078-9
更新日期:2015-01-01 00:00:00
abstract::To investigate patients with severe acute pancreatitis (SAP) by dynamic levels of pro-/anti-inflammatory cytokines and endotoxin (ET) in plasma and the relationship between immunity and infection, organ dysfunction. Seventy-two patients with SAP were recruited. The ET, tumor necrosis factor-α (TNF-α), interleukin-6 (I...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-010-9205-4
更新日期:2011-02-01 00:00:00
abstract::Free radicals play an important role in the initiation and progression of inflammatory bowel disease (IBD). Therefore, the reduction or elimination of adverse oxidant effects can provide novel therapy for IBD. Here, the antioxidant capacity and protective effects of a new class of chemically modified hetastarch (polyn...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1023/a:1014420327417
更新日期:2002-02-01 00:00:00
abstract::Various disease states are associated with eosinophilia and the release of eosinophil peroxidase (EPO) into the microenvironment. The present study targets the effects of low levels of EPO on macrophage (M phi) phagocytosis and intracellular killing of Candida albicans as well as M phi oxidative activity measured as t...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1023/a:1027366119901
更新日期:1997-04-01 00:00:00
abstract::Increased susceptibility of neonates to infection may be related to defects in newborn neutrophil (PMN) functional activities, including altered responses to complement fragments (Cf) and defective microbicidal activity. We therefore compared the kinetics of newborn and adult bovine PMN membrane shape change responses...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF00914305
更新日期:1989-12-01 00:00:00
abstract::Current treatments for ischemia-reperfusion (IR)-induced acute lung injury are limited. Mesenchymal stem cell-conditioned medium (CM) has been reported to attenuate lung injury. Neural crest stem cells (NCSCs), a type of multipotent stem cells, are more easily obtained than mesenchymal stem cells. We hypothesize that ...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-017-0594-5
更新日期:2017-10-01 00:00:00
abstract::Previous reports showed that Maresin 1 (MaR1) possessed organ protection effects and could attenuate acute lung injury. Here, we aim to figure out whether MaR1 can maintain the permeability of lung epithelial cells by regulating the expression of tight junction protein during lung injury. Monolayer of murine lung epit...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-016-0433-0
更新日期:2016-12-01 00:00:00
abstract::The secretion of calcitonin gene-related peptide (CGRP) and the chemokines KC and MIP-2 are increased in the animal models of endotoxemic and septic shock. We tested whether CGRP could modulate KC and MIP-2 secretion from different sources of macrophages after murine sepsis induced by cecal ligation and puncture (CLP)...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1023/a:1016515712344
更新日期:2002-08-01 00:00:00
abstract::Pig alveolar macrophages are a heterogeneous population of cells. Three subpopulations or bands exist when the whole population is separated according to density. Band 1 cells are the least dense cells and constitute 9% of the total population. Bands 2 and 3 represent 44 and 47% of the total population. The three subp...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF00915841
更新日期:1987-09-01 00:00:00
abstract::Ovarian cancer is one of the most deadly cancers with rapid proliferation and poor prognosis among patients. Therapies focusing on regulation of tumor immunity and microenvironments are developing. MiR-217 was dysregulated in cancer progress and plays important roles in tumorigenesis and metastasis. However, the role ...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-019-01004-2
更新日期:2019-10-01 00:00:00
abstract::Salidroside (Sal), a phenylpropanoid glycoside isolated from a popular traditional Chinese medicinal plant Rhodiola rosea L., possesses multiple pharmacological actions. This aim of this study is to investigate the effects of Sal against isoproterenol (ISO)-induced myocardial ischemia. Fifty male Sprague-Dawley rats w...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-015-0134-0
更新日期:2015-08-01 00:00:00
abstract::Different studies have shown the regulatory effects of vitamin D(3) on the immune system and bone metabolism. Regarding the effects of vitamin D on immune cells and the importance of cytokines on bone metabolism, we assessed the association between serum levels of interleukin (IL)-6, IL-10, IL-12, IL-17 and IFN-γ cyto...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-012-9531-9
更新日期:2013-02-01 00:00:00
abstract::Leukocyte chemoattractants markedly alter the morphology and membrane functions of leukocytes. Bacterial collagenase causes a change in cell shape similar to that seen with the leukocyte chemoattractant, f-Met-Leu-Phe, and also promotes capping of concanavalin A. Human neutrophils in suspension or adherent to cover gl...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF00915819
更新日期:1986-12-01 00:00:00
abstract::Praeruptorin C, D, and E (PC, PD, and PE) are three pyranocoumarins isolated from the dried root of Peucedanum praeruptorum Dunn of Umbelliferae. In the present study, we investigated the anti-inflammatory effect of these compounds in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Pyranocoumarins signi...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-011-9400-y
更新日期:2012-06-01 00:00:00
abstract::Parthenolide, a naturally occurring sesquiterpene lactone derived from feverfew (Tanacetum parthenium), exhibits exceptional anti-cancer and anti-inflammatory properties, making it a prominent candidate for further studies and drug development. In this review, we briefly investigate molecular events and cell-specific ...
journal_title:Inflammation
pub_type: 杂志文章,评审
doi:10.1007/s10753-011-9346-0
更新日期:2012-04-01 00:00:00
abstract::In our study, we explored the bidirectional communication via soluble factors between bone cells and endotoxin-stimulated splenic lymphocytes in an in vitro coculture model that mimics the inflammatory environment. Both the ability of lymphocytes to affect differentiation and immune properties of bone cells, osteoblas...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-012-9477-y
更新日期:2012-10-01 00:00:00
abstract::Macrophage polarization is a dynamic and integral process of tissue inflammation and remodeling. Here we demonstrate an important role of Aurora kinase A in the regulation of inflammatory M1 macrophage polarization. We found that there was an elevated expression of Aurora-A in M1 macrophages and inhibition of Aurora-A...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/s10753-014-9990-2
更新日期:2015-04-01 00:00:00
abstract::Exposure of human neutrophils to concanavalin A (Con A) resulted in a time- and concentration-dependent extracellular release of granule-associated lysozyme but not beta-glucuronidase or cytosolic lactate dehydrogenase. Maximum extrusion of lysozyme occurred 30 min after cell contact with Con A. The percent of total g...
journal_title:Inflammation
pub_type: 杂志文章
doi:10.1007/BF00916046
更新日期:1980-12-01 00:00:00