Abstract:
:The possible voltage-dependence of the dopamine transporter (DAT) was investigated using electrophysiological and electrochemical recordings in rat brain slices containing the substantia nigra (SN). Whole-cell patch clamp recordings of DA neurons, revealed that addition of 15 mM KCl rapidly depolarized the membrane potential by approximately 20 mV, whereas these cells were hyperpolarized approximately 10 mV by DA (10 microM) and approximately 14 mV by the GABAB-receptor agonist baclofen (30 microM). High-speed chronoamperometric recordings were used to monitor clearance properties of exogenously applied DA signals during similar manipulations. Superfusion of slices with 15 mM KCl significantly increased the time course of the DA signal, consistent with inhibition of DAT activity. Application of the D2 DA-receptor antagonist sulpiride (50 microM) also significantly increased the time course, suggesting that DA-induced hyperpolarization enhances DAT activity. Baclofen reversed the effects of sulpiride on DA clearance. These results demonstrate that changes in DA cell membrane potential can modulate DAT activity.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Hoffman AF,Zahniser NR,Lupica CR,Gerhardt GAdoi
10.1016/s0304-3940(98)00951-3keywords:
subject
Has Abstractpub_date
1999-01-29 00:00:00pages
105-8issue
2eissn
0304-3940issn
1872-7972pii
S0304-3940(98)00951-3journal_volume
260pub_type
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