Abstract:
:Monovalent cation binding sites on nucleic acids in solution can be localized using the isotopically labeled ammonium ion (15NH4+) as a probe in high resolution NMR spectroscopy experiments. The application of this technique to a series of DNA duplexes reveals a preference for the binding of ammonium cations in the minor groove of A-tract sequences. These results are consistent with a recent report which indicates that some solvent electron densities previously identified as water molecules in DNA X-ray crystal structures are partially occupied by sodium ions. The sequence-specific nature of monovalent cation binding sites demonstrated here for A-tract DNA provides an explanation for the origin of sequence-directed bending.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Hud NV,Sklenár V,Feigon Jdoi
10.1006/jmbi.1998.2513keywords:
subject
Has Abstractpub_date
1999-02-26 00:00:00pages
651-60issue
3eissn
0022-2836issn
1089-8638pii
S0022-2836(98)92513-Xjournal_volume
286pub_type
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