Localization of ammonium ions in the minor groove of DNA duplexes in solution and the origin of DNA A-tract bending.

Abstract:

:Monovalent cation binding sites on nucleic acids in solution can be localized using the isotopically labeled ammonium ion (15NH4+) as a probe in high resolution NMR spectroscopy experiments. The application of this technique to a series of DNA duplexes reveals a preference for the binding of ammonium cations in the minor groove of A-tract sequences. These results are consistent with a recent report which indicates that some solvent electron densities previously identified as water molecules in DNA X-ray crystal structures are partially occupied by sodium ions. The sequence-specific nature of monovalent cation binding sites demonstrated here for A-tract DNA provides an explanation for the origin of sequence-directed bending.

journal_name

J Mol Biol

authors

Hud NV,Sklenár V,Feigon J

doi

10.1006/jmbi.1998.2513

keywords:

subject

Has Abstract

pub_date

1999-02-26 00:00:00

pages

651-60

issue

3

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(98)92513-X

journal_volume

286

pub_type

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