Abstract:
:The aim of this study was to determine the protein pattern of human thyroid fine needle aspiration fluid (FNA) using a proteomic approach. FNA proteins were separated using 2-dimensional gel electrophoresis (2DE), digested and then analyzed by peptide mass fingerprinting. For the first time, we provided an image of the protein components of the FNA, in which approximately 220 protein spots can be identified. The proteome analysis revealed a specific fingerprint of FNA with proteins appertaining to various functional systems. Our preliminary results of FNA protein pattern could be a starting point in studying the presence of potential markers implicated in thyroid diseases.
journal_name
J Endocrinol Investjournal_title
Journal of endocrinological investigationauthors
Giusti L,Iacconi P,Ciregia F,Giannaccini G,Basolo F,Donatini G,Miccoli P,Lucacchini Adoi
10.1007/BF03349229subject
Has Abstractpub_date
2007-11-01 00:00:00pages
865-9issue
10eissn
0391-4097issn
1720-8386pii
4238journal_volume
30pub_type
杂志文章abstract::Insulin degradation in IM-9 cultured human lymphocytes occurs extracellularly. In the presence of 0.1% bovine serum albumin, insulin degradation products are formed that are soluble in 5% trichloroacetic acid. In the presence of 5% bovine serum albumin, the major products of insulin degradation are insoluble in 5% tri...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348223
更新日期:1980-01-01 00:00:00
abstract::The pathogenesis of human obesity is the result of dysregulation of the reciprocal relationship between food intake and energy expenditure (EE), which influences daily energy balance and ultimately leads to weight gain. According to principles of energy homeostasis, a relatively lower EE in a setting of energy balance...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/s40618-017-0732-9
更新日期:2018-01-01 00:00:00
abstract::The aim of the study was to examine the effects of strenuous training on the hypothalamic- pituitary-adrenal axis activity. Exercise tests and saliva collections for analysis of awakening cortisol response (ACR) and midnight cortisol were performed before and after a 7-day period of intensified training in a group of ...
journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章
doi:10.1007/BF03345561
更新日期:2008-01-01 00:00:00
abstract:OBJECTIVE:Although two studies have shown that Addison's disease (AD) is still a potentially lethal condition for cardiovascular, malignant, and infectious diseases, a recent retrospective study showed a normal overall mortality rate. Differently from secondary hypoadrenalism, scanty data exist on the role of conventio...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03345773
更新日期:2009-12-01 00:00:00
abstract:UNLABELLED:Hypercalcemia occurring in patients with advanced breast cancer (BC) is generally due to osteolytic metastases or to the activity of circulating tumor-derived products. In these conditions, the production of endogenous PTH is reduced. The frequency of hypercalcemia due to primary hyperparathyroidism in breas...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03343867
更新日期:2001-05-01 00:00:00
abstract::Basal IGF-I levels and the GH response to at least two among provocative stimuli such as clonidine (CLO, Catapresan, 150 mcg/m2 p.o.), GHRH (1 mcg/kg i.v.)+arginine (ARG, 0.5 g/kg i.v. infusion during 30 min) and GHRH+pyridostigmine (PD, Mestinon cpr 60 mg p.o.) have been evaluated in 43 children with Prader-Willi syn...
journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章
doi:10.1007/BF03343684
更新日期:2000-02-01 00:00:00
abstract:PURPOSE:In patients with obesity, micronutrient deficiencies have been reported both before and after bariatric surgery (BS). Obesity is a chronic pro-inflammatory status, and inflammation increases the risk of micronutrient malnutrition. Our objective was to assess in pre-BS patients the prevalence of micronutrient de...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-020-01439-7
更新日期:2020-10-07 00:00:00
abstract:AIM:In the last years there has been a progressive reduction of the average duration of sleep and an increase in the incidence of sleep disturbances. At the same time, an increase of the incidence of the metabolic syndrome has been described, partly attributable to the progressive worsening of dietary habits and the in...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,随机对照试验
doi:10.1007/s40618-013-0016-y
更新日期:2014-02-01 00:00:00
abstract::Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is a rare, but well-defined monogenic disorder that is caused by mutations in the autoimmune regulator (AIRE) gene on chromosome 21q22.3. Patients most often suffer from loss of endocrine function in the parathyroid and adrenal glands but may also...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/BF03345516
更新日期:2002-10-01 00:00:00
abstract::Unfortunately, the author name and his affiliation were published in the original publication. They have now been removed. ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,已发布勘误
doi:10.1007/s40618-019-01012-x
更新日期:2019-02-01 00:00:00
abstract::Differently from other metabolic conditions, most of calcium metabolism disorders are diagnosed through simple detection of both serum and urinary excretion (24-h urine collection), levels of calcium, total and ionized form, and phosphate, and of calciotropic hormone serum levels, such as calcitonin, PTH and vitamin D...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:
更新日期:2003-01-01 00:00:00
abstract::In a 12-yr-old boy growth arrest occurred after severe head trauma. Evaluation of pituitary endocrine function revealed isolated growth hormone (GH) deficiency. Lack of GH response to GH-releasing factor indicated that the responsible lesion was most likely to be localized in the pituitary gland. GH substitution resul...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349069
更新日期:1988-06-01 00:00:00
abstract::The long-acting somatostatin analogue SMS 201-995 has been used efficaciously in the therapy of metastatic carcinoid tumor, vasoactive intestinal peptide producing islet cell carcinoma, acromegaly, and TSH secreting pituitary tumors. We report the development of a gallstone in a patient treated for 23 months with a lo...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348589
更新日期:1990-01-01 00:00:00
abstract::The Nb2 rat lymphoma bioassay (BA) for prolactin (PRL) was performed in 26 subjects with hyperprolactinemia, 17 of whom had radiologic evidence of a pituitary adenoma. All subjects were treated with the long acting dopamine agonist CV 205-502. The radioimmunoassay (RIA) PRL significantly decreased with treatment but t...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348787
更新日期:1992-07-01 00:00:00
abstract::Severe bleeding into the peritoneal cavity from a ruptured ovulatory follicle is a rare complication in women receiving anticoagulation therapy. A number of both surgical and non surgical methods have been proposed to solve this problem. We adopted a novel therapeutic approach in a 40-year old patient who presented wi...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348998
更新日期:1994-05-01 00:00:00
abstract:INTRODUCTION:Carcinoids are mainly found in the gastrointestinal (65%) and bronchopulmonary tract (25%). These neuroendocrine tumors secrete a wide range of bioactive peptides, including gastrin releasing peptide and neuromedin B, the mammalian analogs of bombesin. The purpose of this study was to investigate the quant...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.3275/7332
更新日期:2011-10-01 00:00:00
abstract::The effects of short term administration of 200 MRC U of synthetic salmon calcitonin (sCT) daily on carbohydrate metabolism were investigated in 10 patients with various bone diseases, 3 of whom had type II diabetes mellitus and 3 of whom had impaired glucose tolerance. Blood glucose levels during the nocturnal postab...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350629
更新日期:1985-02-01 00:00:00
abstract::The status of the erythrocyte insulin receptor was investigated prior to and during storage at 4C in acid-citrate dextrose (ACD) solution. The receptors on cells that were obtained from both resting and exercised fasting subjects and stored for up to three days in ACD were unchanged as evaluated by both maximal specif...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347594
更新日期:1983-08-01 00:00:00
abstract::A 36-year-old non-pregnant woman presented with a four-month history of progressive visual deterioration and amenorrhea. The latest gestation was 6 years earlier. Hormonal study revealed central diabetes insipidus, hypopituitarism, and slightly increased prolactin level. Ophthalmologic examination showed bilateral hem...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03343842
更新日期:2001-03-01 00:00:00
abstract::Prolactin (PRL) circulates as multiple molecular weight variants: glycosylated phosphorylated, deamidated and sulphated forms. The profiles of the forms, as determined by isoelectrofocusing (IEF), differ in physiological and pathological conditions. The case of a 72-year-old woman affected by an invasive prolactinoma ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350303
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND:Mutations of the thyrotropin receptor (TSHR) and/or Gαs gene have been found in a number of, but not all, autonomously functioning thyroid nodules (AFTNs). Recently, in a 15-year-old girl with a hyperfunctioning papillary thyroid carcinoma, we found two somatic and germline single nucleotide polymorphisms (S...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-014-0081-x
更新日期:2014-07-01 00:00:00
abstract::Estrogen deficiency in females and androgen deficiency in males may harm periosteal and endosteal apposition, reduce bone size and both cortical and trabecular thickness, modifying in this way the bone structure later in life. To date, few systematic studies on bone mineral density (BMD) and hypogonadism in adolescent...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03346513
更新日期:2009-07-01 00:00:00
abstract::Plasma T4, T3, and TSH, and total and 131l-labeled T4 and T3 in thyroid digests were determined in male and female rats from birth to 30--45 days postnatally. Mothers and offspring were fed a high-iodine Purina diet throughout. In both sexes, plasma T4 was less than 1 microgram/100ml at birth but rose linearly to a pe...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350975
更新日期:1978-10-01 00:00:00
abstract::In healthy pregnant women, the regulation of thyroid function depends upon several factors. Three factors act independently to increase thyroid hormone requirements: 1) the marked increase in the binding capacity of serum due to high TBG levels; 2) the direct stimulation of the thyroid by human chorionic gonadotropin,...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/BF03348861
更新日期:1993-05-01 00:00:00
abstract::The Authors have studied the effects of somatostatin (SRIF) treatment in an infant affected by hypoglycemia due to nesidioblastosis. During iv infusion with SRIF we observed a marked increase of blood glucose levels; concomitantly insulin secretion was almost completely suppressed. In contrast, during treatment with p...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350454
更新日期:1981-04-01 00:00:00
abstract::The research suggests that adiponectin plays an important role in sensitizing insulin action. It is interesting to find that the lower levels of adiponectin exist in the plasma of obese and Type 2 diabetes subjects and in the adipose tissue of obese, db/db mice, and insulin-resistant individuals. However, the underlyi...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03346688
更新日期:2011-01-01 00:00:00
abstract::Glucocorticoid receptors (GR) and 3-O-methyl-D glucose (3-O-MG) transport were determined in mononuclear leukocytes (MNL) from 11 abdominal obese subjects, 10 pituitary-dependent Cushing's syndrome (Cushing's disease) and 10 healthy controls. Using a whole-cell competitive binding assay and 3H-dexamethasone as tracer,...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350794
更新日期:1998-11-01 00:00:00
abstract:OBJECTIVE:Graves' orbitopathy (GO) is an inflammatory orbital disease of autoimmune origin with the potential to cause severe functional and psychosocial effects. The pathogenesis has not been fully elucidated. We investigated whether DNA methylation was associated with GO incidence in Chinese patients. MATERIALS AND ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-017-0796-6
更新日期:2018-06-01 00:00:00
abstract::Neurological disorders, such as seizures, are not infrequently associated with anti-leukemic therapy. It has been hypothesized that a disrupted peptidergic transmission between neurons could be the cellular basis of the neurological dysfunction. Since endogenous opioids have been recently found to alter neuronal funct...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350022
更新日期:1989-10-01 00:00:00
abstract::Androgen insensitivity is a disorder characterized by an abnormal male sexual development, in which the androgen action is impaired due to structural defects in the androgen receptor gene. We report a case of a 46,XY subject with female phenotype (normal breast and external genitalia) lacking sexual hair, affected wit...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03344130
更新日期:2006-05-01 00:00:00