Hsa_circ_0000199 facilitates chemo-tolerance of triple-negative breast cancer by interfering with miR-206/613-led PI3K/Akt/mTOR signaling.


:Increasing attentions have been paid to the role of circRNAs in the etiology of triple-negative breast cancer (TNBC), and we strived to figure out the association of circRNA AKT3/miRNA axis with TNBC chemo-resistance. Altogether 207 BC patients were divided into TNBC group (n=83) and non-TNBC group (n=124), and MCF-10A, MDA-MB-231, MDA-MB-468, SK-BR-3 and MCF-7 cell lines were prepared in advance. Expressions of AKT3-derived circRNAs and relevant miRNAs in the TNBC tissues and cell lines were determined by employing real-time polymerase chain reaction (PCR). It was indicated that hsa_circ_0000199 expression was higher in TNBC tissues than in non-TNBC tissues, and high hsa_circ_0000199 expression was predictive of large tumor size, advanced TNM grade, high Ki-67 level and poor 3-year survival of TNBC patients (all P<0.05). Furthermore, miR-613 and miR-206 were sponged and negatively regulated by hsa_circ_0000199 (P<0.001), and PI3K/Akt/mTOR signaling was depressed by si-hsa_circ_0000199 in TNBC cell lines (P<0.01). Ultimately, miR-206/miR-613 inhibitor reversed impacts of si-hsa_circ_0000199 on PI3K/Akt/mTOR signaling, proliferation, migration, invasion, chemo-sensitivity and autophagy of TNBC cells (all P<0.01). Conclusively, silencing of hsa_circ_0000199 enhanced TNBC chemo-sensitivity by promoting miR-206/miR-613 expression and deactivating PI3K/Akt/mTOR signaling, which was conducive to improving chemotherapeutic efficacy of TNBC patients.


Aging (Albany NY)




Li H,Xu W,Xia Z,Liu W,Pan G,Ding J,Li J,Wang J,Xie X,Jiang D




Has Abstract


2021-01-20 00:00:00










  • A novel six-microRNA-based model to improve prognosis prediction of breast cancer.

    abstract::Current tumor-node-metastasis (TNM) stage is unable to accurately predict the overall survival (OS) in breast cancer (BC) patients. This study aimed to construct a microRNA (miRNA)-based model to improve survival prediction of BC. We confirmed 99 differentially expressed miRNAs (DEMs) in 1044 BC samples compared to 10...


    pub_type: 杂志文章


    authors: Lai J,Wang H,Pan Z,Su F

    更新日期:2019-01-30 00:00:00

  • Expression and significance of miR - 20b in retinal photoreceptor cells exposed to PCB1254.

    abstract::Previous studies have shown that PCB1254 has an adverse effect on zebrafish retinal development, but the basic mechanism behind it is not clear. The purpose of this study was to investigate the molecular mechanisms of PCB-induced retinal dysplasia. RT-qPCR, immunoblotting, HE staining and immunofluorescence were adopt...


    pub_type: 杂志文章


    authors: Zhang X,Zhang Q,Jiang Y,Zhang S,Hong Q,Guo X,Chi X,Tong M

    更新日期:2019-10-16 00:00:00

  • Mitochondria-targeted antioxidant SkQ1 inhibits age-dependent involution of the thymus in normal and senescence-prone rats.

    abstract::One of the most striking changes during mammal aging is a progressive involution of the thymus, associated with an increase in susceptibility to infections, autoimmune diseases and cancer. In order to delay age-related processes, we have developed mitochondria-targeted antioxidant plastoquinonyl decyltriphenyl phospho...


    pub_type: 杂志文章


    authors: Obukhova LA,Skulachev VP,Kolosova NG

    更新日期:2009-04-22 00:00:00

  • Error-prone nonhomologous end joining repair operates in human pluripotent stem cells during late G2.

    abstract::Genome stability of human embryonic stem cells (hESC) is an important issue because even minor genetic alterations can negatively impact cell functionality and safety. The incorrect repair of DNA double-stranded breaks (DSBs) is the ultimate cause of the formation of chromosomal aberrations. Using G2 radiosensitivity ...


    pub_type: 杂志文章


    authors: Bogomazova AN,Lagarkova MA,Tskhovrebova LV,Shutova MV,Kiselev SL

    更新日期:2011-06-01 00:00:00

  • Brain ageing changes proteoglycan sulfation, rendering perineuronal nets more inhibitory.

    abstract::Chondroitin sulfate (CS) proteoglycans in perineuronal nets (PNNs) from the central nervous system (CNS) are involved in the control of plasticity and memory. Removing PNNs reactivates plasticity and restores memory in models of Alzheimer's disease and ageing. Their actions depend on the glycosaminoglycan (GAG) chains...


    pub_type: 杂志文章


    authors: Foscarin S,Raha-Chowdhury R,Fawcett JW,Kwok JCF

    更新日期:2017-06-28 00:00:00

  • Construction and analysis of a joint diagnosis model of random forest and artificial neural network for heart failure.

    abstract::Heart failure is a global health problem that affects approximately 26 million people worldwide. As conventional diagnostic techniques for heart failure have been in practice with various limitations, it is necessary to develop novel diagnostic models to supplement existing methods. With advances and improvements in g...


    pub_type: 杂志文章


    authors: Tian Y,Yang J,Lan M,Zou T

    更新日期:2020-12-26 00:00:00

  • Mst1 promotes mitochondrial dysfunction and apoptosis in oxidative stress-induced rheumatoid arthritis synoviocytes.

    abstract::In this study, we investigated the role of macrophage stimulating 1 (Mst1) and the AMPK-Sirt1 signaling pathway in the oxidative stress-induced mitochondrial dysfunction and apoptosis seen in rheumatoid arthritis-related fibroblast-like synoviocytes (RA-FLSs). Mst1 mRNA and protein expression was significantly higher ...


    pub_type: 杂志文章


    authors: Wang Y,Yang Q,Shen S,Zhang L,Xiang Y,Weng X

    更新日期:2020-07-21 00:00:00

  • Rapamycin treatment benefits glucose metabolism in mouse models of type 2 diabetes.

    abstract::Numerous studies suggest that rapamycin treatment promotes insulin resistance, implying that rapamycin could have negative effects on patients with, or at risk for, type 2 diabetes (T2D). New evidence, however, indicates that rapamycin treatment produces some benefits to energy metabolism, even in the context of T2D. ...


    pub_type: 杂志文章


    authors: Reifsnyder PC,Flurkey K,Te A,Harrison DE

    更新日期:2016-11-30 00:00:00

  • SLC7A2 serves as a potential biomarker and therapeutic target for ovarian cancer.

    abstract::The solute carrier (SLC) family is the largest group of membrane transporters, but their functions in ovarian cancer (OV) remain unclear. We analyzed SLC family members with amino acids-transporting functions in OV. The mRNA expression levels and prognostic values of SLCs in OV were analyzed in the Gene Expression Pro...


    pub_type: 杂志文章


    authors: Sun T,Bi F,Liu Z,Yang Q

    更新日期:2020-07-09 00:00:00

  • COL6A6 interacted with P4HA3 to suppress the growth and metastasis of pituitary adenoma via blocking PI3K-Akt pathway.

    abstract::The role and mechanism of collagen type VI alpha 6 (COL6A6) on tumor growth and metastasis in pituitary adenoma (PA) was determined. COL6A6 was downregulated in PA tissues and cell lines, which was negatively associated with the expression of prolyl-4-hydroxylase alpha polypeptide III (P4HA3) in the progression of PA....


    pub_type: 杂志文章


    authors: Long R,Liu Z,Li J,Yu H

    更新日期:2019-10-17 00:00:00

  • Improved therapeutic effects on diabetic foot by human mesenchymal stem cells expressing MALAT1 as a sponge for microRNA-205-5p.

    abstract::Diabetic foot (DF) is a common complication of high severity for diabetes, a prevalent metabolic disorder that affects billions of people worldwide. Mesenchymal stem cells (MSCs) have a demonstrative therapeutic effect on DF, through their generation of pro-angiogenesis factors, like vascular endothelial growth factor...


    pub_type: 杂志文章


    authors: Zhu L,Zhong Q,Yang T,Xiao X

    更新日期:2019-12-21 00:00:00

  • Pyridoxine 5'-phosphate oxidase is correlated with human breast invasive ductal carcinoma development.

    abstract::Pyridoxine 5'-phosphate oxidase (PNPO) is a converting enzyme for an active form of vitamin B6. This study aims to evaluate the biological function and the regulatory mechanism of PNPO in human breast invasive ductal carcinoma (IDC). We unveiled for the first time that PNPO was upregulated in patients with IDC and was...


    pub_type: 杂志文章


    authors: Ren W,Guan W,Zhang J,Wang F,Xu G

    更新日期:2019-04-14 00:00:00

  • Discovering markers of healthy aging: a prospective study in a Danish male birth cohort.

    abstract::There is a pressing need to identify markers of cognitive and neural decline in healthy late-midlife participants. We explored the relationship between cross-sectional structural brain-imaging derived phenotypes (IDPs) and cognitive ability, demographic, health and lifestyle factors (non-IDPs). Participants were recru...


    pub_type: 杂志文章


    authors: Zarnani K,Nichols TE,Alfaro-Almagro F,Fagerlund B,Lauritzen M,Rostrup E,Smith SM

    更新日期:2019-08-26 00:00:00

  • Testicular biopsies microarray analysis reveals circRNAs are involved in the pathogenesis of non-obstructive azoospermia.

    abstract::Circular RNAs (circRNAs) have been reported to be involved in many diseases. But there is no report on circRNAs in non-obstructive azoospermia (NOA). The purpose of this paper is to explore the circular RNA expression profile and potential functions of circRNAs in NOA patients. We first preformed circRNA expression pr...


    pub_type: 杂志文章


    authors: Bo H,Liu Z,Tang R,Gong G,Wang X,Zhang H,Zhu F,Zhou D,Zhu W,Tan Y,Fan L

    更新日期:2020-02-06 00:00:00

  • Caveolin-1, cellular senescence and pulmonary emphysema.

    abstract::Caveolae are vesicular invaginations of the plasma membrane. Caveolin-1 is the structural protein component of caveolae. Caveolin-1 participates in signal transduction processes by acting as a scaffolding protein that concentrates, organizes and functional regulates signaling molecules within caveolar membranes. Cigar...


    pub_type: 杂志文章


    authors: Volonte D,Galbiati F

    更新日期:2009-08-10 00:00:00

  • Atorvastatin reduces cerebral vasospasm and infarction after aneurysmal subarachnoid hemorrhage in elderly Chinese adults.

    abstract::We explored whether acute atorvastatin treatment would improve clinical outcomes and reduce the incidence of cerebral vasospasm after aneurysmal subarachnoid hemorrhage in elderly Chinese adults. Patients (60 to 90 years old) were admitted to intensive care units after surgery to clip or embolize their aneurysms. We a...


    pub_type: 杂志文章,随机对照试验


    authors: Chen J,Li M,Zhu X,Chen L,Yang S,Zhang C,Wu T,Feng X,Wang Y,Chen Q

    更新日期:2020-02-07 00:00:00

  • Obstructive sleep apnea syndrome and causal relationship with female breast cancer: a mendelian randomization study.

    abstract::Although observational studies have reported a positive association between obstructive sleep apnea syndrome (OSAS) and breast cancer (BC) risk, causality remains inconclusive. We aim to explore whether OSAS is associated with etiology of BC by conducting a two-sample Mendelian randomization (MR) study in a Chinese po...


    pub_type: 杂志文章


    authors: Gao XL,Jia ZM,Zhao FF,An DD,Wang B,Cheng EJ,Chen Y,Gong JN,Liu D,Huang YQ,Yang JJ,Wang SJ

    更新日期:2020-02-29 00:00:00

  • Single-cell expression profiles of ACE2 and TMPRSS2 reveals potential vertical transmission and fetus infection of SARS-CoV-2.

    abstract::Morbidity and mortality of coronavirus disease 2019 (COVID-19) is age-dependent. It remains unclear whether vertical severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurs during pregnancy and how such infection will affect fetal development. Here, we performed single-cell transcriptomic analysis of place...


    pub_type: 杂志文章


    authors: Lü M,Qiu L,Jia G,Guo R,Leng Q

    更新日期:2020-10-26 00:00:00

  • Annotation of susceptibility SNPs associated with atrial fibrillation.

    abstract:OBJECTIVE:Genome-wide association studies (GWAS) and the candidate gene based association studies have identified a panel of variants associated with atrial fibrillation (AF), however, most of the identified single nucleotide polymorphisms (SNPs) were found located within intergenic or intronic genomic regions, and whe...


    pub_type: 杂志文章


    authors: Xu C,Zhang R,Xia Y,Xiong L,Yang W,Wang P

    更新日期:2020-09-09 00:00:00

  • Acetate attenuates perioperative neurocognitive disorders in aged mice.

    abstract::Perioperative neurocognitive disorders are common in elderly patients who have undergone surgical procedures. Neuroinflammation induced by microglial activation is a hallmark of these neurological disorders. Acetate can suppress inflammation in the context of inflammatory diseases. We employed an exploratory laparotom...


    pub_type: 杂志文章


    authors: Wen C,Xie T,Pan K,Deng Y,Zhao Z,Li N,Bian J,Deng X,Zha Y

    更新日期:2020-02-26 00:00:00

  • Identification of an 88-microRNA signature in whole blood for diagnosis of hepatocellular carcinoma and other chronic liver diseases.

    abstract::Hepatocellular carcinoma (HCC) is a common cancer with very poor survival due to lack of reliable biomarker for early diagnosis. In this study, we investigated microRNA (miRNA) profile of whole blood with a custom microarray containing probes for 1849 miRNA species in a total 213 successive subjects who were divided i...


    pub_type: 杂志文章


    authors: Long XR,Zhang YJ,Zhang MY,Chen K,Zheng XFS,Wang HY

    更新日期:2017-06-27 00:00:00

  • The mechanism of submandibular gland dysfunction after menopause may be associated with the ferroptosis.

    abstract::Salivary gland dysfunction is a common symptom that occurs after menopause. This study was performed to investigate the mechanism of salivary gland dysfunction to confirm the relationship between ferroptosis and salivary gland dysfunction by ovariectomy. Forty-eight female rats were randomly divided into four groups (...


    pub_type: 杂志文章


    authors: Kwon HK,Kim JM,Shin SC,Sung ES,Kim HS,Park GC,Cheon YI,Lee JC,Lee BJ

    更新日期:2020-11-05 00:00:00

  • Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma.

    abstract::Head and neck squamous cell carcinoma (HNSCC), a major histologic subtype of head and neck cancer, presents great mortality and morbidity worldwide. The aim of this study is to discover new potential biomarkers closely correlated with HNSCC progression. In this study, weighted gene co-expression network analysis was a...


    pub_type: 杂志文章


    authors: Xu W,Zou H,Wei Z,Song C,Tang C,Yin X,Wang Y,Han S,Cai Y,Han W

    更新日期:2019-06-06 00:00:00

  • Analysis of liver injury factors in 332 patients with COVID-19 in Shanghai, China.

    abstract:OBJECTIVE:We analyzed clinical parameters and risk factors for coronavirus disease 2019 (COVID-19)-related liver damage. RESULTS:Of the 332 COVID-19 patients, 306 and 26 were included in the non-critical and critical groups, respectively. The median time from onset to admission was 4.0 (2.0-8.0) days. Of the 332 COVID...


    pub_type: 杂志文章


    authors: Guo H,Zhang Z,Zhang Y,Liu Y,Wang J,Qian Z,Zou Y,Lu H

    更新日期:2020-10-01 00:00:00

  • Overexpression of CBS and CSE genes affects lifespan, stress resistance and locomotor activity in Drosophila melanogaster.

    abstract::Recent experimental studies highlighted the role of hydrogen sulfide (H2S) in aging and longevity. The cystathionine ß-synthase (CBS) and cystathionine γ-lyase (CSE) are the key enzymes responsible for H2S production. Here we investigated the geroprotective effects of CSE and CBS overexpression in Drosophila. Overexpr...


    pub_type: 杂志文章


    authors: Shaposhnikov M,Proshkina E,Koval L,Zemskaya N,Zhavoronkov A,Moskalev A

    更新日期:2018-11-08 00:00:00

  • Aging and circadian disruption: causes and effects.

    abstract::The relationship between aging and daily "circadian" behavior in humans is bidirectional: on the one hand, dysfunction of circadian clocks promotes age-related maladies; on the other, aging per se leads to changes and disruption in circadian behavior and physiology. For the latter case, recent research suggests that c...


    pub_type: 杂志文章


    authors: Brown SA,Schmitt K,Eckert A

    更新日期:2011-08-01 00:00:00

  • SNCA Rep1 microsatellite length influences non-motor symptoms in early Parkinson's disease.

    abstract::Long alpha-synuclein gene (SNCA) promoter (Rep1) allele-carriers are linked to higher risk for Parkinson's disease (PD) and faster motor progression. Non-motor symptoms including autonomic, neuropsychiatric, and sleep disorders are common in PD. However, the relationship between SNCA Rep1 microsatellite lengths and no...


    pub_type: 杂志文章


    authors: Yong AC,Tan YJ,Zhao Y,Lu Z,Ng EY,Ng SY,Chia NS,Choi X,Heng D,Neo S,Xu Z,Tay KY,Au WL,Tan EK,Tan LC,Ng AS

    更新日期:2020-10-20 00:00:00

  • Prognostic roles of metabolic reprogramming-associated genes in patients with hepatocellular carcinoma.

    abstract::Metabolic reprogramming for adaptation to the tumor microenvironment is recognized as a hallmark of cancer. Although many altered metabolic genes have been reported to be associated with tumor pathological processes, systematic analysis of metabolic genes implicated in hepatocellular carcinoma prognosis remains rare. ...


    pub_type: 杂志文章


    authors: Cui L,Xue H,Wen Z,Lu Z,Liu Y,Zhang Y

    更新日期:2020-11-12 00:00:00

  • Calreticulin increases growth and progression of natural killer/T-cell lymphoma.

    abstract::In this study, we investigated the role of calreticulin (CALR) in the pathogenesis of natural killer/T-cell lymphoma (NKTCL). CALR expression was significantly higher in the NKTCL tissues than normal control tissues in the GSE80632 dataset. High CALR expression correlated with poorer overall survival of NKTCL patients...


    pub_type: 杂志文章


    authors: Zheng Y,Li C,Xin P,Peng Q,Zhang W,Liu S,Zhu X

    更新日期:2020-11-18 00:00:00

  • Activation of adenosine A3 receptor reduces early brain injury by alleviating neuroinflammation after subarachnoid hemorrhage in elderly rats.

    abstract::The incidence of subarachnoid hemorrhage (SAH) and hazard ratio of death increase with age. Overactivation of microglia contributes to brain damage. This study aimed to investigate the effects of A3 adenosine receptors (A3R) activation on neurofunction and microglial phenotype polarization in the context of SAH in age...


    pub_type: 杂志文章


    authors: Li P,Li X,Deng P,Wang D,Bai X,Li Y,Luo C,Belguise K,Wang X,Wei X,Xia Z,Yi B

    更新日期:2020-11-30 00:00:00