Downregulation of miR-155-5p enhances the anti-tumor effect of cetuximab on triple-negative breast cancer cells via inducing cell apoptosis and pyroptosis.

Abstract:

:Cetuximab resistance is the main obstacle for the treatment of EGFR overexpression cancer, including triple-negative breast cancer (TNBC). MicroRNA (miRNA)-155-5p is upregulated in TNBC cells; thus, the present study explored whether the downregulation of miR-155-5p enhanced the anti-tumor effect of cetuximab in TNBC cells. MDA-MB-231 and MDA-MB-468 cells were infected with lentivirus-epidermal growth factor receptor (EGFR) for 72 h to obtain EGFR-overexpressed cell lines (MDA-MB-231 and MDA-MB-468). The inhibitory effects of cetuximab on the proliferation and migration of EGFR-overexpressed MDA-MB-468 cells were enhanced following transfection with the miR-155-5p antagomir, and miR-155-5p knockdown enhanced the pro-apoptotic effect of cetuximab on EGFR-overexpressed MDA-MB-468 cells. Further, the luciferase reporter assay revealed that gasdermin E (GSDME) was the direct binding target of miR-155-5p. The combination of cetuximab with the miR-155-5p antagomir promoted pyroptosis in EGFR-overexpressed MDA-MB-468 cells via the upregulation of GSDME-N and cleaved caspase-1. Results from the in vivo experiments confirmed that the downregulation of miR-155-5p enhanced the anti-tumor effect of cetuximab in an MDA-MB-468 xenograft model and on EGFR-overexpressed TNBC cells via inducing cell apoptosis and pyroptosis. Therefore, cetuximab combination with an miR-155-5p antagomir may be a novel therapeutic strategy for the treatment of TNBC.

journal_name

Aging (Albany NY)

journal_title

Aging

authors

Xu W,Song C,Wang X,Li Y,Bai X,Liang X,Wu J,Liu J

doi

10.18632/aging.103669

subject

Has Abstract

pub_date

2021-01-05 00:00:00

pages

228-240

issue

1

issn

1945-4589

pii

103669

journal_volume

13

pub_type

杂志文章

相关文献

Aging-US文献大全
  • PKCζ-dependent upregulation of p27kip1 contributes to oxidative stress induced retinal pigment epithelial cell multinucleation.

    abstract::Retinal pigment epithelial (RPE) cells increase in size and multinucleate during aging. We have shown using human and mouse cell lines that oxidised photoreceptor outer segments (oxPOS)-induced cytokinesis failure is related to RPE cell multinucleation, although the underlying mechanism remains unknown. This study inv...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.101299

    authors: Rajapakse D,Chen M,Curtis TM,Xu H

    更新日期:2017-10-09 00:00:00

  • Downregulation of interleukin-6 and C-reactive protein underlies a novel inhibitory role of microRNA-136-5p in acute lower extremity deep vein thrombosis.

    abstract::Deep vein thrombosis (DVT) comprises a critical and common health condition with high incidence, mortality, and long-term adverse sequelae. Several differentially expressed microRNAs (miRNAs) have emerged as promising prognostic markers in DVT. The present study intended to explore the functional relevance of miR-136-...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.103140

    authors: Ou M,Hao S,Chen J,Zhao S,Cui S,Tu J

    更新日期:2020-11-14 00:00:00

  • Genes associated with Type 2 Diabetes and vascular complications.

    abstract::Type 2 Diabetes (T2D) is a chronic disease associated with a number of micro- and macrovascular complications that increase the morbidity and mortality of patients. The risk of diabetic complications has a strong genetic component. To this end, we sought to evaluate the association of 40 single nucleotide polymorphism...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.101375

    authors: Montesanto A,Bonfigli AR,Crocco P,Garagnani P,De Luca M,Boemi M,Marasco E,Pirazzini C,Giuliani C,Franceschi C,Passarino G,Testa R,Olivieri F,Rose G

    更新日期:2018-02-04 00:00:00

  • SAG expression associates with COPB2-related signaling and a poorer prognosis in breast cancer.

    abstract::SAG is an essential RING component of the Cullin-RING ligase (CRL) E3 ubiquitin ligase complex, which regulates diverse signaling pathways and biological processes, including cell apoptosis, embryonic development, angiogenesis, and tumorigenesis. In the present study, we revealed that SAG gene expression is upregulate...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.102663

    authors: Liu A,Zhang S,Li W,Xu B,Lei R,Zhu S

    更新日期:2020-01-11 00:00:00

  • Association of VAMP5 and MCC genetic polymorphisms with increased risk of Hirschsprung disease susceptibility in Southern Chinese children.

    abstract::Hirschsprung disease (HSCR) is a genetic disorder characterized by the absence of neural crest cells in parts of the intestine. This study aims to investigate the association of vesicle-associated membrane protein 5 (VAMP5) and mutated in colorectal cancer (MCC) genetic polymorphisms and their correlated risks with HS...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.101423

    authors: Zhao J,Xie X,Yao Y,He Q,Zhang R,Xia H,Zhang Y

    更新日期:2018-04-25 00:00:00

  • Prevention of diet-induced hepatic steatosis and hepatic insulin resistance by second generation antisense oligonucleotides targeted to the longevity gene mIndy (Slc13a5).

    abstract::Reducing the expression of the Indy (I'm Not Dead Yet) gene in lower organisms extends life span by mechanisms resembling caloric restriction. Similarly, deletion of the mammalian homolog, mIndy (Slc13a5), encoding for a plasma membrane tricarboxylate transporter, protects from aging- and diet-induced adiposity and in...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.100854

    authors: Pesta DH,Perry RJ,Guebre-Egziabher F,Zhang D,Jurczak M,Fischer-Rosinsky A,Daniels MA,Willmes DM,Bhanot S,Bornstein SR,Knauf F,Samuel VT,Shulman GI,Birkenfeld AL

    更新日期:2015-12-01 00:00:00

  • Cognitive frailty in relation to adverse health outcomes independent of multimorbidity: results from the China health and retirement longitudinal study.

    abstract::Our objectives were to evaluate: 1) the associations of cognitive frailty with various health outcomes including disability, hospitalization, and death; 2) whether the associations differed by multimorbidity. We included data of 5113 Chinese older adults (aged 60+ years) who had baseline cognition and physical frailty...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.104078

    authors: Chen C,Park J,Wu C,Xue Q,Agogo G,Han L,Hoogendijk EO,Liu Z,Wu Z

    更新日期:2020-11-18 00:00:00

  • Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice.

    abstract::Sarcopenia is the degenerative loss of muscle mass and strength with aging. Although a role of mitochondrial metabolism in muscle function and in the development of many diseases has been described, the role of mitochondrial topology and dynamics in the process of muscle aging is not fully understood. This work shows ...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.101358

    authors: Del Campo A,Contreras-Hernández I,Castro-Sepúlveda M,Campos CA,Figueroa R,Tevy MF,Eisner V,Casas M,Jaimovich E

    更新日期:2018-01-04 00:00:00

  • FX5 as a non-steroidal GR antagonist improved glucose homeostasis in type 2 diabetic mice via GR/HNF4α/miR-122-5p pathway.

    abstract::Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease characterized by glucose metabolic disorders, and gluconeogenesis inhibiting is a promisingly therapeutic strategy for T2DM. Glucocorticoid receptor (GR) is tightly implicated in the regulation of gluconeogenesis, although the underlying mechanism remains ...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.202275

    authors: Xu X,Chen Y,Zhu D,Zhao T,Xu R,Wang J,Hu L,Shen X

    更新日期:2020-12-09 00:00:00

  • SNRPB-mediated RNA splicing drives tumor cell proliferation and stemness in hepatocellular carcinoma.

    abstract::Hepatocellular carcinoma (HCC) is one of the leading malignant diseases worldwide, but therapeutic targets for HCC are lacking. Here, we characterized a significant upregulation of Small Nuclear Ribonucleoprotein Polypeptides B and B1 (SNRPB) in HCC via qRT-PCR, western blotting, tissue microarray and public database ...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.202164

    authors: Zhan YT,Li L,Zeng TT,Zhou NN,Guan XY,Li Y

    更新日期:2020-12-03 00:00:00

  • XRCC1 gene polymorphisms and risk of neuroblastoma in Chinese children.

    abstract::Neuroblastoma is a common pediatric extra-cranial tumor of the sympathetic nervous system. XRCC1 is a scaffold protein that participates in DNA single-strand break repair by complexing with other proteins. XRCC1 gene polymorphisms are being increasingly explored in cancer epidemiology studies. However, the contributio...

    journal_title:Aging

    pub_type: 杂志文章,多中心研究

    doi:10.18632/aging.101601

    authors: Zhang J,Zhuo Z,Li W,Zhu J,He J,Su J

    更新日期:2018-10-25 00:00:00

  • Association between immune-related adverse events and efficacy of PD-1 inhibitors in Chinese patients with advanced melanoma.

    abstract::Programmed cell death 1 (PD-1) checkpoint inhibitor therapy leads to immune-related adverse events (irAEs). We sought to evaluate whether the development of irAEs correlates with the treatment response in Chinese patients with advanced melanoma. In this study, we conducted a retrospective study of advanced melanoma pa...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.103285

    authors: Zhao JJ,Wen XZ,Ding Y,Li DD,Zhu BY,Li JJ,Weng DS,Zhang X,Zhang XS

    更新日期:2020-06-08 00:00:00

  • Single-cell expression profiles of ACE2 and TMPRSS2 reveals potential vertical transmission and fetus infection of SARS-CoV-2.

    abstract::Morbidity and mortality of coronavirus disease 2019 (COVID-19) is age-dependent. It remains unclear whether vertical severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) occurs during pregnancy and how such infection will affect fetal development. Here, we performed single-cell transcriptomic analysis of place...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.104015

    authors: Lü M,Qiu L,Jia G,Guo R,Leng Q

    更新日期:2020-10-26 00:00:00

  • Aging dysregulates D- and E-series resolvins to modulate cardiosplenic and cardiorenal network following myocardial infarction.

    abstract::Post-myocardial infarction (MI), overactive inflammation is the hallmark of aging, however, the mechanism is unclear. We hypothesized that excess influx of omega 6 fatty acids may impair resolution, thus impacting the cardiosplenic and cardiorenal network post-MI. Young and aging mice were fed on standard lab chow (LC...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.101077

    authors: Halade GV,Kain V,Black LM,Prabhu SD,Ingle KA

    更新日期:2016-10-18 00:00:00

  • Beyond the social gradient: the role of lifelong socioeconomic status in older adults' health trajectories.

    abstract::Inequalities in older adults' health rarely consider life-course aspects of socioeconomic status (SES). We examined the association between lifelong SES and old-age health trajectories, and explored the role of lifestyle factors and depressive symptoms in this association. We followed 2760 adults aged 60+ from the Swe...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.202342

    authors: Harber-Aschan L,Calderón-Larrañaga A,Darin-Mattson A,Hu X,Fratiglioni L,Dekhtyar S

    更新日期:2020-12-21 00:00:00

  • Association of KRAS and NRAS gene polymorphisms with Wilms tumor risk: a four-center case-control study.

    abstract::Wilms tumor is a type of pediatric solid tumor that arises partly due to somatic and germline mutations. Single-nucleotide polymorphisms (SNPs) in the RAS gene reportedly modify the risk for several types of human malignancies. We conducted a multicenter study to investigate whether RAS gene variants predispose indivi...

    journal_title:Aging

    pub_type: 杂志文章,多中心研究

    doi:10.18632/aging.101855

    authors: Fu W,Zhuo Z,Hua RX,Fu K,Jia W,Zhu J,Zhang J,Cheng J,Zhou H,Xia H,He J,Liu G

    更新日期:2019-03-12 00:00:00

  • Aging: Dial M for Mitochondria.

    abstract::A major goal of aging research is to identify interventions that prolong lifespan in distantly related organisms. In recent years, genetic studies in both nematodes and rodents have reported that moderate inactivation of genes important for mitochondrial electron transport chain (ETC) function can promote longevity. W...

    journal_title:Aging

    pub_type: 杂志文章,评审

    doi:10.18632/aging.100118

    authors: Hur JH,Cho J,Walker DW

    更新日期:2010-01-26 00:00:00

  • PaCATB, a secreted catalase protecting Podospora anserina against exogenous oxidative stress.

    abstract::A differential mass spectrometry analysis of secreted proteins from juvenile and senescent Podospora anserina cultures revealed age-related differences in protein profiles. Among other proteins with decreased abundance in the secretome of senescent cultures a catalase, termed PaCATB, was identified. Genetic modulation...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.100360

    authors: Zintel S,Bernhardt D,Rogowska-Wrzesinska A,Osiewacz HD

    更新日期:2011-08-01 00:00:00

  • Leptin acts on mesenchymal stem cells to promote chemoresistance in osteosarcoma cells.

    abstract::Leptin signaling influences osteoblastogenesis and modulates the fate of mesenchymal stem cells (MSCs) during bone and cartilage regeneration. Although MSCs abound in the osteosarcoma (OS) microenvironment, and leptin exhibits pro-tumorigenic properties, leptin's influence on OS progression and chemoresistant signalin...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.103027

    authors: Feng H,Zhang Q,Zhao Y,Zhao L,Shan B

    更新日期:2020-04-14 00:00:00

  • Error-prone nonhomologous end joining repair operates in human pluripotent stem cells during late G2.

    abstract::Genome stability of human embryonic stem cells (hESC) is an important issue because even minor genetic alterations can negatively impact cell functionality and safety. The incorrect repair of DNA double-stranded breaks (DSBs) is the ultimate cause of the formation of chromosomal aberrations. Using G2 radiosensitivity ...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.100336

    authors: Bogomazova AN,Lagarkova MA,Tskhovrebova LV,Shutova MV,Kiselev SL

    更新日期:2011-06-01 00:00:00

  • Pharmacological blockade of TNFα prevents sarcopenia and prolongs survival in aging mice.

    abstract::Sarcopenia is a hallmark of aging. Inflammation due to increased generation of cytokines such as TNFα, IL-1β and IL-6 has been implicated in the pathogenesis of sarcopenia. In skeletal muscle of C57BL/6 mice from 12 until 28 months of age, we observed a progressive reduction of myofiber cross sectional area, loss of t...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.202200

    authors: Sciorati C,Gamberale R,Monno A,Citterio L,Lanzani C,De Lorenzo R,Ramirez GA,Esposito A,Manunta P,Manfredi AA,Rovere-Querini P

    更新日期:2020-11-26 00:00:00

  • ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas.

    abstract:BACKGROUND:Hepatocellular carcinomas (HCC) constantly rank among the malignancies with the highest death tolls on the global scale. Moreover, HCC are associated with a limited set of therapeutic options. This is particularly true in the case of advanced stage cancers, where long-term survival is uncommon. For the inope...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.102292

    authors: Chen D,Feng X,Lv Z,Xu X,Lu Y,Wu W,Wu H,Liu H,Cao L,Ye S,Chen J,Wu J

    更新日期:2019-10-25 00:00:00

  • Survival of irradiated recipient mice after transplantation of bone marrow from young, old and "early aging" mice.

    abstract::Bone marrow transplantation is used to examine survival, hematopoietic stem cell function and pathology in recipients of young and old wild type bone marrow derived stem cells (BMDSCs) as well as cells from p53-based models of premature aging. There is no difference in the long term survival of recipients of 8 week-ol...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.100867

    authors: Guest I,Ilic Z,Scrable H,Sell S

    更新日期:2015-12-01 00:00:00

  • p21 can be a barrier to ferroptosis independent of p53.

    abstract::Traditionally, the p21 protein has been viewed as limiting cancer progression and promoting aging. In contrast, there are reports that p21 can enhance cancer survival and limit tissue damage, depending on the tissue of origin and type of stressor involved. Here, we provide evidence to support these latter two roles of...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.103961

    authors: Venkatesh D,Stockwell BR,Prives C

    更新日期:2020-09-24 00:00:00

  • Role of FUT8 expression in clinicopathology and patient survival for various malignant tumor types: A systematic review and meta-analysis.

    abstract::Dysregulation of α(1,6)-fucosyltransferase (FUT8) plays significant roles in development of a variety of malignant tumor types. We collected as many relevant articles and microarray datasets as possible to assess the prognostic value of FUT8 expression in malignant tumors. For this purpose, we systematically searched ...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.202239

    authors: Ma M,Han G,Wang Y,Zhao Z,Guan F,Li X

    更新日期:2020-12-11 00:00:00

  • Identification of prognostic immune-related genes in the tumor microenvironment of endometrial cancer.

    abstract::Endometrial cancer (EC) is one of the most common gynecologic malignancies. To identify potential prognostic biomarkers for EC, we analyzed the relationship between the EC tumor microenvironment and gene expression profiles. Using the ESTIMATE R tool, we found that immune and stromal scores correlated with clinical da...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.102817

    authors: Chen P,Yang Y,Zhang Y,Jiang S,Li X,Wan J

    更新日期:2020-02-19 00:00:00

  • Subtype-specific risk models for accurately predicting the prognosis of breast cancer using differentially expressed autophagy-related genes.

    abstract::Emerging evidence suggests that the dysregulation of autophagy-related genes (ARGs) is coupled with the carcinogenesis and progression of breast cancer (BRCA). We constructed three subtype-specific risk models using differentially expressed ARGs. In Luminal, Her-2, and Basal-like BRCA, four- (BIRC5, PARP1, ATG9B, and ...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.103437

    authors: Han B,Zhang H,Zhu Y,Han X,Wang Z,Gao Z,Yuan Y,Tian R,Zhang F,Niu R

    更新日期:2020-07-10 00:00:00

  • Resistin enhances angiogenesis in osteosarcoma via the MAPK signaling pathway.

    abstract::Over the last two decades, there have been no significant changes in patient outcomes in relation to the treatment of osteosarcoma, an aggressive malignant neoplasm. It is known that vascular endothelial growth factor-A (VEGF-A) plays a crucial role in angiogenesis and in osteosarcoma. Moreover, VEGF-A expression corr...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.102423

    authors: Tsai HC,Cheng SP,Han CK,Huang YL,Wang SW,Lee JJ,Lai CT,Fong YC,Tang CH

    更新日期:2019-11-13 00:00:00

  • Fighting the storm: could novel anti-TNFα and anti-IL-6 C. sativa cultivars tame cytokine storm in COVID-19?

    abstract::The main aspects of severe COVID-19 disease pathogenesis include hyper-induction of proinflammatory cytokines, also known as 'cytokine storm', that precedes acute respiratory distress syndrome (ARDS) and often leads to death. COVID-19 patients often suffer from lung fibrosis, a serious and untreatable condition. There...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.202500

    authors: Kovalchuk A,Wang B,Li D,Rodriguez-Juarez R,Ilnytskyy S,Kovalchuk I,Kovalchuk O

    更新日期:2021-01-19 00:00:00

  • Downregulation of hsa_circ_0007580 inhibits non-small cell lung cancer tumorigenesis by reducing miR-545-3p sponging.

    abstract::Non-small cell lung cancer (NSCLC) is a highly malignant tumor. Many circular RNAs (circRNAs) are reportedly in regulating the progression of NSCLC. To identify potential therapeutic targets for NSCLC, we conducted a bioinformatics analysis of circRNAs differentially expressed between NSCLC tissues and adjacent normal...

    journal_title:Aging

    pub_type: 杂志文章

    doi:10.18632/aging.103472

    authors: Chen S,Lu S,Yao Y,Chen J,Yang G,Tu L,Zhang Z,Zhang J,Chen L

    更新日期:2020-07-18 00:00:00