Abstract:
:Progranulin (PGRN) is a tightly regulated, secreted glycoprotein involved in a wide range of biological processes that is of tremendous interest to the scientific community due to its involvement in neoplastic, neurodevelopmental, and neurodegenerative diseases. In particular, progranulin haploinsufficiency leads to frontotemporal dementia. While performing experiments with a HIS-tagged recombinant human (rh) PGRN protein, we observed a measurable depletion of protein from solution due to its adsorption onto polypropylene (PPE) microcentrifuge tubes. In this study, we have quantified the extent of rhPGRN adsorption to PPE tubes while varying experimental conditions, including incubation time and temperature. We found that ∼25-35% of rhPGRN becomes adsorbed to the surface of PPE tubes even after a short incubation period. We then directly showed the deleterious impact of PGRN adsorption in functional assays and have recommended alternative labware to minimize these effects. Although the risk of adsorption of some purified proteins and peptides to polymer plastics has been characterized previously, this is the first report of rhPGRN adsorption. Moreover, since PGRN is currently being studied and utilized in both basic science laboratories to perform in vitro studies and translational laboratories to survey PGRN as a quantitative dementia biomarker and potential replacement therapy, the reported observations here are broadly impactful and will likely significantly affect the design and interpretation of future experiments centered on progranulin biology.
journal_name
Front Neuroscijournal_title
Frontiers in neuroscienceauthors
Gururaj S,Sampognaro PJ,Argouarch AR,Kao AWdoi
10.3389/fnins.2020.602235subject
Has Abstractpub_date
2020-12-14 00:00:00pages
602235eissn
1662-4548issn
1662-453Xjournal_volume
14pub_type
杂志文章abstract::Neuromorphic computing employs models of neuronal circuits to solve computing problems. Neuromorphic hardware systems are now becoming more widely available and "neuromorphic algorithms" are being developed. As they are maturing toward deployment in general research environments, it becomes important to assess and com...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2015.00491
更新日期:2016-01-08 00:00:00
abstract::The efficacy of neuronal function requires a well-balanced extracellular ion homeostasis and a steady supply with nutrients and metabolites. Therefore, all organisms equipped with a complex nervous system developed a so-called blood-brain barrier, protecting it from an uncontrolled entry of solutes, metabolites or pat...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章,评审
doi:10.3389/fnins.2014.00365
更新日期:2014-11-14 00:00:00
abstract::The molecular mechanism underlying Parkinson's disease (PD), an increasingly common neurodegenerative disease, remains unclear. Long non-coding RNA (lncRNA) plays essential roles in gene expression and human diseases. We hypothesize that lncRNAs are involved in neuronal degeneration of PD. Using microarray, we identif...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2019.01320
更新日期:2019-12-13 00:00:00
abstract::Electroencephalography (EEG) is perhaps the most widely used brain-imaging technique for pediatric populations. However, EEG signals are prone to distortion by motion. Compared to adults, infants' motion is both more frequent and less stereotypical yet motion effects on the infant EEG signal are largely undocumented. ...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.00352
更新日期:2020-04-28 00:00:00
abstract::Oxylipins are a series of bioactive lipid metabolites derived from polyunsaturated fatty acids that are involved in cerebral homeostasis and the development of intracerebral hemorrhage (ICH). However, comprehensive quantification of the oxylipin profile in ICH remains unknown. Therefore, an ICH mouse model was constru...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.00777
更新日期:2020-09-23 00:00:00
abstract::Increasing evidence suggests that noncoding RNAs play key roles in cellular processes, particularly in the brain. The present study used RNA sequencing to identify the transcriptional landscape of two human neural progenitor cell lines, SK-N-SH and ReNcell CX, as they differentiate into human cortical projection neuro...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2015.00392
更新日期:2015-10-23 00:00:00
abstract::Mutations in leucine-rich repeat kinase 2 (LRRK2) instigate an autosomal dominant form of Parkinson's disease (PD). Despite the neuropathological heterogeneity observed in LRRK2-PD, accumulating evidence suggests that alpha-synuclein and tau pathology are observed in a vast majority of cases. Intriguingly, the presenc...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章,评审
doi:10.3389/fnins.2020.00719
更新日期:2020-07-08 00:00:00
abstract::Antiepileptic drugs (AEDs) require daily ingestion for maximal seizure prophylaxis. Adverse psychiatric consequences of AEDs present as: (i) reversible changes in mood, anxiety, anger and/or irritability that often necessitate drug discontinuation, and (ii) autism and/or cognitive/psychomotor delays following fetal ex...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.594612
更新日期:2020-11-06 00:00:00
abstract::Diffusion magnetic resonance imaging (dMRI) is a compelling tool for investigating the structure and geometry of brain tissue based on indirect measurement of the diffusion anisotropy of water. Recent developments in global top-down tractogram optimizations enable the estimation of streamline weights, which characteri...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2017.00729
更新日期:2017-12-22 00:00:00
abstract:BACKGROUND:With the advent of the GeneChip Exon Arrays, it is now possible to extract "exon-level" expression estimates, allowing for detection of alternative splicing events, one of the primary mechanisms of transcript diversity. In the context of (1) a complex trait use case and (2) a human cerebellum vs. heart compa...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2011.00069
更新日期:2011-05-13 00:00:00
abstract::Highlights Simultaneous epileptiform LFPs and single-cell activity can be recorded in the membrane chamber.Interneuron firing can be linked to epileptiform high frequency activity.Fast ripples, unique to chronic epilepsy, can be modeled in ex vivo tissue from TeNT-treated rats. Traditionally, visually-guided patch cla...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2016.00519
更新日期:2016-11-09 00:00:00
abstract::The aim of this work was to test if a novel transcranial direct current stimulation (tDCS) montage boosts the accuracy of lower limb motor imagery (MI) detection by using a real-time brain-machine interface (BMI) based on electroencephalographic (EEG) signals. The tDCS montage designed was composed of two anodes and o...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2018.00757
更新日期:2018-10-23 00:00:00
abstract::Motor neurons (MN) degeneration is a main feature of amyotrophic lateral sclerosis (ALS), a neurological disorder with a progressive course. The diagnosis of ALS is essentially a clinical one. Most common symptoms include a gradual neurological deterioration that reflect the impairment and subsequent loss of muscle fu...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章,评审
doi:10.3389/fnins.2017.00591
更新日期:2017-10-24 00:00:00
abstract::Aggressive behavior is associated with dysfunctional frontolimbic emotion regulation circuits. Recent findings suggest serotonin as a primary transmitter for prefrontal amygdala control. However, the association between serotonin levels, amygdala regulation, and aggression is still a matter of debate. Neurobehavioral ...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2019.00042
更新日期:2019-02-22 00:00:00
abstract::Acupuncture is a traditional Chinese medicine treatment that has widely been used to modulate gastrointestinal dysfunction caused by irritable bowel syndrome (IBS) and to alleviate the resulting pain. Recent studies have shown that gastrointestinal dysfunction caused by IBS is associated with dysregulation of the brai...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.608688
更新日期:2020-12-15 00:00:00
abstract::[This corrects the article DOI: 10.3389/fnins.2019.00413.]. ...
journal_title:Frontiers in neuroscience
pub_type: 已发布勘误
doi:10.3389/fnins.2019.00910
更新日期:2019-08-28 00:00:00
abstract::The structural organization of cortical areas is not random, with topographic maps commonplace in sensory processing centers. This topographical organization allows optimal wiring between neurons, multimodal sensory integration, and performs input dimensionality reduction. In this work, a model of topographic map form...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2018.00434
更新日期:2018-07-02 00:00:00
abstract::Ongoing brain activity has been implicated in the modulation of cortical excitability. The combination of electroencephalography (EEG) and transcranial magnetic stimulation (TMS) in a real-time triggered setup is a novel method for testing hypotheses about the relationship between spontaneous neuronal oscillations, co...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2018.00954
更新日期:2018-12-13 00:00:00
abstract::Poisoning with organophosphorus compounds (OPCs) is a major problem worldwide. Standard therapy with atropine and established oxime-type enzyme reactivators (pralidoxime, obidoxime) is unsatisfactory. In search of more efficacious broad-spectrum oximes, new bispyridinium (K-) oximes have been synthesized, with K027 be...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章,评审
doi:10.3389/fnins.2019.00427
更新日期:2019-05-22 00:00:00
abstract::Early detection of movement intention could possibly minimize the delays in the activation of neuroprosthetic devices. As yet, single trial analysis using non-invasive approaches for understanding such movement preparation remains a challenging task. We studied the feasibility of predicting movement directions in self...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2014.00222
更新日期:2014-08-01 00:00:00
abstract::The melanin-concentrating hormone (MCH) system is a robust integrator of exogenous and endogenous information, modulating arousal and energy balance in mammals. Its predominant function in teleosts, however, is to concentrate melanin in the scales, contributing to the adaptive color change observed in several teleost ...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章,评审
doi:10.3389/fnins.2019.01280
更新日期:2019-11-26 00:00:00
abstract::We present a novel assistive control strategy for a robotic hip exoskeleton for assisting hip flexion/extension, based on a proportional Electromyography (EMG) strategy. The novelty of the proposed controller relies on the use of the Gastrocnemius Medialis (GM) EMG signal instead of a hip flexor muscle, to control the...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2018.00071
更新日期:2018-02-14 00:00:00
abstract::Spatial group independent component analysis (GICA) methods decompose multiple-subject functional magnetic resonance imaging (fMRI) data into a linear mixture of spatially independent components (ICs), some of which are subsequently characterized as brain functional networks. Group information guided independent compo...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2017.00267
更新日期:2017-05-19 00:00:00
abstract::Voltage-gated calcium channels (VGCCs) are important mediators of pain hypersensitivity during inflammatory states, but their role in sensory nerve growth remains underexplored. Here, we assess the role of the N-type calcium channel Cav2.2 in the complete Freund's adjuvant (CFA) model of inflammatory pain. We demonstr...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2019.01009
更新日期:2019-09-19 00:00:00
abstract::Micro-Graphitic Single Crystal Diamond Multi Electrode Arrays (μG-SCD-MEAs) have so far been used as amperometric sensors to detect catecholamines from chromaffin cells and adrenal gland slices. Besides having time resolution and sensitivity that are comparable with carbon fiber electrodes, that represent the gold sta...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2019.00288
更新日期:2019-04-09 00:00:00
abstract::Background: Significant progress toward the recovery of useful vision in blind patients with severe degenerative retinal diseases caused by photoreceptor death has been achieved with the development of visual prostheses that stimulate the retina electrically. However, currently used prostheses do not provide feedback ...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2019.00367
更新日期:2019-04-30 00:00:00
abstract::Background: Stroke is the second leading cause of death worldwide. Angiogenesis facilitates the formation of microvascular networks and promotes recovery after stroke. The Shh/Gli signaling pathway is implicated in angiogenesis and cerebral ischemia-reperfusion (I/R) injury. This study aimed at investigating the influ...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2019.00321
更新日期:2019-04-09 00:00:00
abstract::Regaining communication abilities in patients who are unable to speak or move is one of the main goals in decoding brain waves for brain-computer interface (BCI) control. Many BCI approaches designed for communication rely on attention to visual stimuli, commonly applying an oddball paradigm, and require both eye move...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.591777
更新日期:2020-12-01 00:00:00
abstract::We present a spiking neural network model of the thalamic Lateral Geniculate Nucleus (LGN) developed on SpiNNaker, which is a state-of-the-art digital neuromorphic hardware built with very-low-power ARM processors. The parallel, event-based data processing in SpiNNaker makes it viable for building massively parallel n...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2017.00454
更新日期:2017-08-09 00:00:00
abstract::The finding that the adult mammalian brain is still capable of producing neurons has ignited a new field of research aiming to identify the molecular mechanisms regulating adult neurogenesis. An improved understanding of these mechanisms could lead to the development of novel approaches to delay cognitive decline and ...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章,评审
doi:10.3389/fnins.2018.00707
更新日期:2018-11-05 00:00:00