Accuracy of endoscopic ultrasound-guided needle aspiration specimens for molecular diagnosis of non-small-cell lung carcinoma.

Abstract:

BACKGROUND:Endoscopic ultrasonography-guided fine-needle aspiration (EUS-FNA) and endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) are highly sensitive for diagnosing and staging lung cancer. In recent years, targeted therapy has shown great significance in the treatment of non-small cell lung carcinoma (NSCLC). Using these minimally invasive techniques to obtain specimens for molecular testing will provide patients with a more convenient diagnostic approach. AIM:To evaluate the feasibility and accuracy of tissue samples obtained using EUS-FNA and EBUS-TBNA for molecular diagnosis of NSCLC. METHODS:A total of 83 patients with NSCLC underwent molecular testing using tissues obtained from EUS-FNA or EBUS-TBNA at the Tianjin Medical University Cancer Hospital from January 2017 to June 2019. All enrolled patients underwent chest computed tomography or positron emission tomography/computed tomography prior to puncture. We detected abnormal expression of EGFR, KRAS, MET, HER2, ROS1 and anaplastic lymphoma kinase protein. Two patients failed to complete molecular testing due to insufficient tumor tissue. The clinical features, puncture records, molecular testing results and targeted treatment in the remaining 81 patients were summarized. RESULTS:In a total of 99 tissue samples obtained from 83 patients, molecular testing was successfully completed in 93 samples with a sample adequacy ratio of 93.9% (93/99). Biopsy samples from two patients failed to provide test results due to insufficient tumor tissue. In the remaining 81 patients, 62 cases (76.5%) were found to have adenocarcinoma, 11 cases (13.6%) had squamous cell carcinoma, 3 cases (3.7%) had adenosquamous carcinoma and 5 cases (6.2%) had NSCLC-not otherwise specified. The results of molecular testing showed EGFR mutations in 21 cases (25.9%), KRAS mutations in 9 cases (11.1%), ROS-1 rearrangement in 1 case (1.2%) and anaplastic lymphoma kinase-positive in 5 cases (6.2%). Twenty-four patients with positive results received targeted therapy. The total effectiveness rate of targeted therapy was 66.7% (16/24), and the disease control rate was 83.3% (20/24). CONCLUSION:Tissue samples obtained by EUS-FNA or EBUS-TBNA are feasible for the molecular diagnosis of NSCLC and can provide reliable evidence for clinical diagnosis and treatment.

journal_name

World J Clin Cases

authors

Su W,Tian XD,Liu P,Zhou DJ,Cao FL

doi

10.12998/wjcc.v8.i21.5139

subject

Has Abstract

pub_date

2020-11-06 00:00:00

pages

5139-5148

issue

21

issn

2307-8960

journal_volume

8

pub_type

杂志文章
  • Sinusoidal obstruction syndrome: A systematic review of etiologies, clinical symptoms, and magnetic resonance imaging features.

    abstract:BACKGROUND:Sinusoidal obstruction syndrome (SOS) is a kind of rare liver disease which is characterized by damage to small hepatic vessels, affecting particularly the sinusoidal endothelium. Due to the special etiology and high mortality, early diagnosis of SOS is significant for clinical survival and prognosis. AIM:T...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章

    doi:10.12998/wjcc.v7.i18.2746

    authors: Zhang Y,Jiang HY,Wei Y,Song B

    更新日期:2019-09-26 00:00:00

  • Relationship between homocysteine level and prognosis of elderly patients with acute ischemic stroke treated by thrombolysis with recombinant tissue plasminogen activator.

    abstract:BACKGROUND:Acute ischemic stroke (AIS) is mainly caused by cerebral blood flow disorders, which further leads to ischemic brain necrosis or encephalomalacia. The role of homocysteine (Hcy), an independent risk factor for cardiovascular disease, in the development of atherosclerosis is gradually revealed. However, studi...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章

    doi:10.12998/wjcc.v7.i22.3751

    authors: Li J,Zhou F,Wu FX

    更新日期:2019-11-26 00:00:00

  • Resolution of hemolysis from pump thrombus during left ventricular assist device exchange.

    abstract::A 50-year-old male who underwent a HeartMate II left ventricular assist device placement for ischemic cardiomyopathy presented with discolored urine and hemolysis 3 mo after the operation. His hemolysis was thought to be due to thrombosis within the pump. Imaging studies were not able to visualize a left ventricular t...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v2.i8.373

    authors: Unai S,Hirose H,Entwistle JW 3rd,Samuels LE

    更新日期:2014-08-16 00:00:00

  • CLAG-M chemotherapy followed by umbilical cord blood stem cell transplantation for primary refractory acute myeloid leukaemia in a child: A case report.

    abstract:BACKGROUND:The prognosis of paediatric primary refractory/relapsed acute myeloid leukaemia (R/R AML) remains poor. Intensive therapy is typically used as salvage treatment for those with R/R AML. No data are currently available about the use of the CLAG-M protocol as salvage therapy in paediatric patients with R/R AML....

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v8.i22.5603

    authors: Huang J,Yang XY,Rong LC,Xue Y,Zhu J,Fang YJ

    更新日期:2020-11-26 00:00:00

  • Safety of fecal microbiota transplantation in Chinese children: A single-center retrospective study.

    abstract:BACKGROUND:Fecal microbiota transplantation (FMT) is the administration of fecal bacterial liquid from healthy donors to a recipient's digestive tract, which is recommended as a therapeutic method for recurrent Clostridium difficile infection (CDI). Many clinical trials focusing on different diseases are in progress. T...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章

    doi:10.12998/wjcc.v6.i16.1121

    authors: Zhang XY,Wang YZ,Li XL,Hu H,Liu HF,Li D,Xiao YM,Zhang T

    更新日期:2018-12-26 00:00:00

  • Attitudes, knowledge levels and behaviors of Islamic religious officials about organ donation in Turkey: National survey study.

    abstract:BACKGROUND:The most important factors affecting the decision of organ donation are socioeconomic, educational, cultural, and religious factors. AIM:To evaluate Islamic religious officials' attitudes and behaviors toward and level of knowledge on organ donation and transplantation. METHODS:This study surveyed 2350 Isl...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章

    doi:10.12998/wjcc.v8.i9.1620

    authors: Akbulut S,Ozer A,Firinci B,Saritas H,Demyati K,Yilmaz S

    更新日期:2020-05-06 00:00:00

  • Pneumomediastinum after acute lymphoblastic leukemia and chemotherapy?

    abstract::Pneumomediastinum, pneumorachis and subcutaneous emphysema are frequently benign and most commonly result from air escaping from the upper respiratory tract, intrathoracic airways, or gastrointestinal tract. Gas can also be generated by certain infections or reach the mediastinal space from outside air after trauma or...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章

    doi:10.12998/wjcc.v2.i5.170

    authors: Cruz-Portelles A

    更新日期:2014-05-16 00:00:00

  • Synchronous parathyroid adenoma, papillary thyroid carcinoma and thyroid adenoma in pregnancy: A case report.

    abstract:BACKGROUND:There is a common pathologic relationship between parathyroid adenoma and thyroid cancer, but this relationship is infrequent in pregnant patients with primary hyperparathyroidism (PHPT). CASE SUMMARY:A 27-year-old gravida 1 woman was transferred to our hospital at 16 wk of pregnancy. She was diagnosed with...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v8.i21.5426

    authors: Li Q,Xu XZ,Shi JH

    更新日期:2020-11-06 00:00:00

  • Autoimmune hepatitis in a patient with immunoglobulin A nephropathy: A case report.

    abstract:BACKGROUND:Immunoglobulin A nephropathy (IgAN) is the most commonly encountered glomerular disease in Asian countries. It has a broad clinical presentation, and it is frequently associated with other conditions. Chronic liver disease is well recognized as the leading cause of secondary IgAN. However, cases of IgAN asso...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v8.i17.3828

    authors: Jeon YH,Kim DW,Lee SJ,Park YJ,Kim HJ,Han M,Kim IY,Lee DW,Song SH,Lee SB,Seong EY

    更新日期:2020-09-06 00:00:00

  • Gene testing for osteonecrosis of the femoral head in systemic lupus erythematosus using targeted next-generation sequencing: A pilot study.

    abstract:BACKGROUND:Previous publications indicated that genetic predisposition might play important roles in the onset of osteonecrosis of the femoral head (ONFH) in systemic lupus erythematosus (SLE). Some gene loci such as complement C3d receptor 2 (CR2), nitric oxide synthase 3 (NOS3), collagen type II alpha 1 chain (COL2A1...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章

    doi:10.12998/wjcc.v8.i12.2530

    authors: Sun HS,Yang QR,Bai YY,Hu NW,Liu DX,Qin CY

    更新日期:2020-06-26 00:00:00

  • Acetaminophen-induced acute pancreatitis: A case report and literature review.

    abstract::Acute pancreatitis is rarely associated with drugs. Acetaminophen overdose is a well-known cause of hepatic toxicity, but drug-induced pancreatitis is rarely reported, especially after mild overdose. A 32-year-old woman presented with nausea and vomiting for 12 h, but no abdominal pain following an overdose of eight T...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v6.i9.291

    authors: He YH,Lu L,Wang YF,Huang JS,Zhu WQ,Guo Y,Li CX,Li HM

    更新日期:2018-09-06 00:00:00

  • Robot-assisted retroperitoneal laparoscopic excision of perirenal vascular tumor: A case report.

    abstract:BACKGROUND:A vascular tumor is a benign tumor with unique clinical and pathological features. Perirenal vascular tumor is extremely rare and has not yet been reported. Clinically, it manifests as soreness and swelling. Color ultrasound and renal angiography illustrated the perirenal mass, which was closely connected wi...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v8.i10.2050

    authors: Zhang C,Fu B,Xu S,Zhou XC,Cheng XF,Fu WQ,Wang GX

    更新日期:2020-05-26 00:00:00

  • Dysbetalipoproteinemia: Two cases report and a diagnostic algorithm.

    abstract::Dysbetalipoproteinemia is a rare familial dyslipidemia characterized by approximately equally elevated serum cholesterol and triglyceride levels due to accumulated remnant lipoproteins in apolipoprotein E2/E2 homozygotes. It is associated with an increased risk for premature cardiovascular disease. Thus, making a diag...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v3.i4.371

    authors: Kei A,Miltiadous G,Bairaktari E,Hadjivassiliou M,Cariolou M,Elisaf M

    更新日期:2015-04-16 00:00:00

  • Application of endoscopic submucosal dissection in duodenal space-occupying lesions.

    abstract:BACKGROUND:Endoscopic submucosal dissection (ESD) has been advocated by digestive endoscopists because of its comparable therapeutic effect to surgery, reduced trauma, faster recovery, and fewer complications. However, ESD for lesions of the duodenum is more challenging than those occurring at other levels of the gastr...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章

    doi:10.12998/wjcc.v8.i24.6296

    authors: Li XY,Ji KY,Qu YH,Zheng JJ,Guo YJ,Zhang CP,Zhang KP

    更新日期:2020-12-26 00:00:00

  • DNA methylation detection methods used in colorectal cancer.

    abstract::Colorectal cancer (CRC) remains a major contributor to the number of cancer-related deaths that occur annually worldwide. With the development of molecular biology methods, an increasing number of molecular biomarkers have been identified and investigated. CRC is believed to result from an accumulation of epigenetic c...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章,评审

    doi:10.12998/wjcc.v7.i19.2916

    authors: Zhan YX,Luo GH

    更新日期:2019-10-06 00:00:00

  • Role of quorum sensing in bacterial infections.

    abstract::Quorum sensing (QS) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation, and swarming motility once a population threshold is reached. Several lines of evidence indicate tha...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章,评审

    doi:10.12998/wjcc.v3.i7.575

    authors: Castillo-Juárez I,Maeda T,Mandujano-Tinoco EA,Tomás M,Pérez-Eretza B,García-Contreras SJ,Wood TK,García-Contreras R

    更新日期:2015-07-16 00:00:00

  • Coexistence of ovarian serous papillary cystadenofibroma and type A insulin resistance syndrome in a 14-year-old girl: A case report.

    abstract:BACKGROUND:Type A insulin resistance syndrome is a rare disorder caused by mutations in the gene encoding the insulin receptor. Its coexistence with ovarian serous papillary cystadenofibroma is even rarer. CASE SUMMARY:A 14-year-old girl developed type A insulin resistance syndrome and showed high fasting insulin, glu...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v8.i15.3334

    authors: Yan FF,Huang BK,Chen YL,Zhuang YZ,You XY,Liu CQ,Li XJ

    更新日期:2020-08-06 00:00:00

  • Diagnostic-therapeutic management of bile duct cancer.

    abstract::Biliary tract cancer, or cholangiocarcinoma, comprises a heterogeneous group of malignant tumors that can emerge at any part of the biliary tree. This group is the second most common type of primary liver cancer. Diagnosis is usually based on symptoms, which may be heterogeneous, and nonspecific biomarkers in serum an...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章,评审

    doi:10.12998/wjcc.v7.i14.1732

    authors: Huguet JM,Lobo M,Labrador JM,Boix C,Albert C,Ferrer-Barceló L,Durá AB,Suárez P,Iranzo I,Gil-Raga M,de Burgos CB,Sempere J

    更新日期:2019-07-26 00:00:00

  • Severe isolated sciatic neuropathy due to a modified lotus position.

    abstract::A 51-year-old man presented to our hospital with progressive pain and weakness in his right leg. Neurological examination revealed atrophy of all muscles of the right leg, unilateral foot drop and paralysis of the anterior tibial and gastrocnemicus muscles. Electromyography confirmed a severe isolated sciatic neuropat...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v2.i2.39

    authors: Bosma JW,Wijntjes J,Hilgevoord TA,Veenstra J

    更新日期:2014-02-16 00:00:00

  • Mediastinal small cell carcinoma with liver and bone marrow metastasis, mimicking lymphoma.

    abstract::Primary mediastinal neuroendocrine tumors are a rare malignancy that accounts for < 10% of all mediastinal tumors. The case presented here involves a 52-year-old man who had been suffering for 3 mo from chronic cough, anorexia and substantial weight loss, as well as 2 wk of jaundice prior to his admission. A computed ...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v3.i10.915

    authors: Nawarawong N,Pongpruttipan T,Aswakul P,Prachayakul V

    更新日期:2015-10-16 00:00:00

  • Splenic hamartoma: A case report and review of the literature.

    abstract::Splenic hamartoma is a rare benign malformation, composed of an anomalous mixture of normal splenic elements, often found incidentally while working up other complaints or at autopsy. A splenic mass was incidentally found while evaluating the effects of a traffic accident in a 63-year-old woman. Abdominal computed tom...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v1.i7.217

    authors: Sim J,Ahn HI,Han H,Jun YJ,Rehman A,Jang SM,Jang K,Paik SS

    更新日期:2013-10-16 00:00:00

  • Congenital bronchobiliary fistula: A case report and review of the literature.

    abstract:BACKGROUND:Congenital bronchobiliary fistula is a rare developmental abnormality with an abnormal fistula between the respiratory system and biliary tract. The aim of this report is to analyze and summarize the clinical features and experience of diagnosing and treating congenital bronchobiliary fistula (CBBF) occurrin...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v7.i7.881

    authors: Li TY,Zhang ZB

    更新日期:2019-04-06 00:00:00

  • Association between resting energy expenditure, psychopathology and HPA-axis in eating disorders.

    abstract:AIM:To investigate the complex relationships between resting energy expenditure (REE), eating psychopathology, and Hypothalamus Pituitary Adrenal axis functioning in patients with eating disorders. METHODS:The study was designed as a cross-sectional survey, and it was planned by the Clinic for Eating Disorders of the ...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章

    doi:10.12998/wjcc.v2.i7.257

    authors: Castellini G,Castellani W,Lelli L,Sauro CL,Dini C,Lazzeretti L,Bencini L,Mannucci E,Ricca V

    更新日期:2014-07-16 00:00:00

  • Laparoscopic hysterectomy as optimal approach for 5400 grams uterus with associated polycythemia: A case report.

    abstract:BACKGROUND:Uterine myomas are the most common benign gynecological soft tissue tumors in women but polycythemia associated with these tumors is rare. Polycythemia is significantly associated with risk of venous thromboembolism (VTE), which is further increased in case of a large pelvic mass and obesity. Here we report ...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v7.i19.3027

    authors: Macciò A,Chiappe G,Lavra F,Sanna E,Nieddu R,Madeddu C

    更新日期:2019-10-06 00:00:00

  • Choice and management of negative pressure drainage in anterior cervical surgery.

    abstract:BACKGROUND:Postoperative unobstructed drainage is an important measure for avoiding hematoma formation and preventing complications from anterior cervical surgery. AIM:To discuss the characteristics and key points of clinical management of two types of commonly used negative pressure drainage systems in clinical setti...

    journal_title:World journal of clinical cases

    pub_type: 杂志文章

    doi:10.12998/wjcc.v8.i11.2201

    authors: Su QH,Zhu K,Li YC,Chen T,Zhang Y,Tan J,Guo S

    更新日期:2020-06-06 00:00:00

  • Hodgkin's lymphoma coexisting with liver failure secondary to acute on chronic hepatitis B.

    abstract::Acute on chronic liver failure (ACLF) is rarely the initial manifestation of a malignant process or precipitated by the initiation of anti-viral treatment with a nucleoside or nucleotide agent. We report an unusual case of ACLF temporally associated with initiation of Entecavir for treatment of chronic hepatitis B. Ea...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v1.i1.37

    authors: Palta R,McClune A,Esrason K

    更新日期:2013-04-16 00:00:00

  • Pedunculated colonic lipoma prolapsing through the anus.

    abstract::Colorectal lipomas are the second most common benign tumors of the colon. These masses are typically incidental findings with over 94% being asymptomatic. Symptoms-classically abdominal pain, bleeding per rectum and alterations in bowel habits-may arise when lipomas become larger than 2 cm in size. Colonic lipomas are...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v3.i5.457

    authors: Ghanem OM,Slater J,Singh P,Heitmiller RF,DiRocco JD

    更新日期:2015-05-16 00:00:00

  • Adult-onset mitochondrial encephalopathy in association with the MT-ND3 T10158C mutation exhibits unique characteristics: A case report.

    abstract:BACKGROUND:Mitochondrial diseases are a heterogenous group of multisystemic disorders caused by genetic mutations affecting mitochondrial oxidation function. Brain involvement is commonly found in most cases but rarely as the unique clinical manifestation. Since the knowledge of its clinical manifestation combined with...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v7.i9.1066

    authors: Fu XL,Zhou XX,Shi Z,Zheng WC

    更新日期:2019-05-06 00:00:00

  • Diffuse large B-cell lymphoma arising from follicular lymphoma with warthin's tumor of the parotid gland - immunophenotypic and genetic features: A case report.

    abstract:BACKGROUND:Warthin's tumor (WT) is composed of several cysts that are lined with tall, bilayered oncocytic columnar cells and lymphoid stroma. Within WT, the two components rarely transform into carcinoma or lymphoma, and when it does, carcinoma is the most common type. Approximately 28 cases of lymphoma with WT have b...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v7.i22.3895

    authors: Wang CS,Chu X,Yang D,Ren L,Meng NL,Lv XX,Yun T,Cao YS

    更新日期:2019-11-26 00:00:00

  • Type A aortic dissection developed after type B dissection with the presentation of shoulder pain: A case report.

    abstract:BACKGROUND:Aortic dissection (AD) is a life-threatening condition with a high mortality rate without immediate medical attention. Early diagnosis and appropriate treatment are critical in treating patients with AD. In the emergency department, patients with AD commonly present with classic symptoms of unanticipated sev...

    journal_title:World journal of clinical cases

    pub_type:

    doi:10.12998/wjcc.v9.i1.232

    authors: Yin XB,Wang XK,Xu S,He CY

    更新日期:2021-01-06 00:00:00