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The Yield and Consistency of the Detection of SARS-CoV-2 in Multiple Respiratory Specimens.

Abstract：

Background:Few studies have compared the yield of reverse transcription polymerase chain reaction (RT-PCR) assays in nasopharyngeal swabs, oropharyngeal swabs, and sputum for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection. Methods:We conducted an observational study in Beijing Ditan Hospital, China. Specimens including nasopharyngeal swabs, oropharyngeal swabs, and sputum from confirmed coronavirus 2019 patients were collected for RT-PCR testing. Disease duration was calculated from the date of symptom onset to the date of specimen collection and divided into 3 groups: ≤14 days, 14-21 days, and >21 days. We compared positive rates across the 3 specimens collected. The kappa coefficient was used to evaluate the consistency of RT-PCR results between different specimens. Results:A total of 291 specimens were collected and tested from 43 confirmed patients. Among specimens collected with a disease duration of ≤14 days, the positive rate was highest in sputum (79.2%); this rate was significantly higher than that in nasopharyngeal swabs (37.5%; P = .003) and oropharyngeal swabs (20.8%; P < .001). Similar findings were observed with the disease durations of 14-21 days and >21 days. The consistency of testing results between nasopharyngeal swabs and oropharyngeal swabs was low with the disease durations of ≤14 days and >21 days. The consistency between the sputum and oropharyngeal swabs and between the sputum and nasopharyngeal swabs was very low across all 3 disease durations, with statistical significance. Conclusions:Compared with nasopharyngeal swabs and oropharyngeal swabs, sputum had the highest yield of SARS-CoV-2 detection. Nasopharyngeal swabs and oropharyngeal swabs had a similar yield. If sputum is not feasible, a nasopharyngeal swab can be recommended for the detection of SARS-CoV-2, and early testing is needed.

journal_name

Open Forum Infect Dis

journal_title

Open forum infectious diseases

authors

Zhang H,Chen M,Zhang Y,Wen J,Wang Y,Wang L,Guo J,Liu C,Li D,Wang Y,Bai J,Gao G,Wang S,Yang D,Yu F,Yan L,Wan G,Zhang F

doi

10.1093/ofid/ofaa379

subject

Has Abstract

pub_date

2020-08-26 00:00:00

pages

ofaa379

issue

issn

2328-8957

pii

ofaa379

journal_volume

pub_type

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The Yield and Consistency of the Detection of SARS-CoV-2 in Multiple Respiratory Specimens.