Abstract:
:Hepatoblastoma (HB) is a malignant embryonal tumor of the liver that consists of heterogenous populations of stem/progenitor cells. Although circular RNAs (circRNAs) play an essential role in tumor development, the effects of circRNA on the proliferation of HB cells, especially cancer stem cells (CSCs), remain unclear. We found that the circRNA, CDR1as, was highly expressed in CSC-enriched populations of HB cell lines. Results from flow cytometric and sphere-forming assays revealed that CDR1as knockdown in HB cell lines decreased the proportion of stem cells. The Cell Counting Kit-8 (CCK-8) assay, colony formation experiments, and EdU assay revealed that CDR1as knockdown in HB cell lines decreased cell growth and the colony-forming abilities. Biotin-coupled probe pull-down assays and biotin-coupled microRNA capture were conducted to evaluate the interaction between CDR1as and miR-7-5p. Dual-luciferase reporter assays demonstrated that Kruppel-like factor 4 (KLF4), expression of which is highly correlated with cancer stemness, was a target of miR-7-5p. Overall, the knockdown of CDR1as significantly inhibited the proliferation and stemness of HB cells by reducing the sponge activity on miR-7-5p and subsequently suppressing the interaction between miR-7-5p and KLF4. Results from this study suggest that CDR1as is an oncogene that effects the proliferation and stemness of HBs.
journal_name
Aging (Albany NY)journal_title
Agingauthors
Chen L,Shi J,Wu Y,Qiu R,Zeng L,Lou L,Su J,Liao M,Deng Xdoi
10.18632/aging.103748subject
Has Abstractpub_date
2020-10-14 00:00:00pages
19233-19253issue
19issn
1945-4589pii
103748journal_volume
12pub_type
杂志文章相关文献
Aging-US文献大全abstract::Macromitophagy controls mitochondrial quality and quantity. It involves the sequestration of dysfunctional or excessive mitochondria within double-membrane autophagosomes, which then fuse with the vacuole/lysosome to deliver these mitochondria for degradation. To investigate a physiological role of macromitophagy in y...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100547
更新日期:2013-04-01 00:00:00
abstract::Recent studies have demonstrated circular RNAs (circRNAs) to be widely expressed and to have important physiological functions. However, the expression, regulation, and function of circRNAs in neuroglial cells are unknown. Herein, we characterized the expression, regulation, and function of circRNAs in astrocytes. Ast...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102529
更新日期:2019-12-20 00:00:00
abstract::Alternative splicing (AS) changes are considered to be critical in predicting treatment response. Our study aimed to investigate differential splicing patterns and to elucidate the role of splicing factor (SF) as prognostic markers of low-grade glioma (LGG). We downloaded RNA-seq data from a cohort of 516 LGG tumors i...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103491
更新日期:2020-07-13 00:00:00
abstract::Metabolic syndrome, a network of medical disorders that greatly increase the risk for developing metabolic and cardiovascular diseases, has reached epidemic levels in many areas of today's world. Despite this alarming medicare situation, scientific understandings on the root mechanisms of metabolic syndrome are still ...
journal_title:Aging
pub_type: 杂志文章,评审
doi:10.18632/aging.100431
更新日期:2012-02-01 00:00:00
abstract::Telomeres are short segments in chromosome ends, the length of which is reduced during cell lifecycles. We examined the association of mean leukocyte telomere length (LTL) and short telomere proportion (STP) with hemodynamic variables in normotensive and never-treated hypertensive volunteers (n=566, 19-72 years). STP ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103459
更新日期:2020-06-23 00:00:00
abstract::Myocardial infarction is a cardiovascular disease with high mortality. Human umbilical cord mesenchymal stem cells (hUC-MSCs) with strong self-renewal capacity and multipotency, provide the possibility of replacing injured cardiomyocytes. hUC-MSCs were cultured on polyacrylamide hydrogels with stiffnesses correspondin...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202244
更新日期:2020-12-09 00:00:00
abstract::Supporting cells (SCs) of the cochlear (auditory) and vestibular (balance) organs hold promise as a platform for therapeutic regeneration of the sensory hair cells. Prior data have shown proliferative restrictions of adult SCs forced to re-enter the cell cycle. By comparing juvenile and adult SCs in explant cultures, ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100668
更新日期:2014-06-01 00:00:00
abstract::DNA damaging agents and radiation, cytotoxins and anti-cancer drugs, telomere erosion and cytokines, culture shock and mitogenic stimuli, oncogenes and tumor suppressors can induce both cell cycle arrest and cellular senescence. Due to this semi-coincidence, senescence is confused with cell cycle arrest, or even more ...
journal_title:Aging
pub_type: 杂志文章,评审
doi:10.18632/aging.100281
更新日期:2011-02-01 00:00:00
abstract::Age can significantly affect human physiology and disease risk. Recent studies have shown that age may affect the composition and function of the gut microbiota, but the underlying mechanisms remain largely unknown. Non-human primates are an ideal model for uncovering how age shapes the gut microbiota, as their microb...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102541
更新日期:2019-12-14 00:00:00
abstract::Family with sequence similarity 83, member A (FAM83A), as a potential tumor promoter, was reported to contribute to the progression of several malignant tumors. However, the significance of FAM83A in invasion and metastasis of non-small cell lung cancer (NSCLC) remains largely unknown. In this study, we found that FAM...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102163
更新日期:2019-08-24 00:00:00
abstract::In this study, we investigated the role of calreticulin (CALR) in the pathogenesis of natural killer/T-cell lymphoma (NKTCL). CALR expression was significantly higher in the NKTCL tissues than normal control tissues in the GSE80632 dataset. High CALR expression correlated with poorer overall survival of NKTCL patients...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104030
更新日期:2020-11-18 00:00:00
abstract::Differences in microRNA (miRNA) expression after intracerebral hemorrhage (ICH) have been reported in human and animal models, and miRNAs are being investigated as a new treatment for inflammation and oxidative stress after ICH. In this study, we found that microRNA-183-5p expression was decreased in the mouse brain a...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103343
更新日期:2020-06-29 00:00:00
abstract::Alterations in KEAP1/ NF-E2 p45-related factor 2 (NFE2L2/Nrf2) signaling pathway have been reported in 23% lung adenocarcinoma patients, suggesting that deregulation of the pathway is a major cancer driver. Here we report that mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) drives tumor growth and drug re...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102531
更新日期:2019-12-06 00:00:00
abstract::Atg7, a critical component of autophagy machinery, is essential for counteracting hematopoietic aging. However, the non-autophagic role of Atg7 on hematopoietic cells remains fundamentally unclear. In this study, we found that loss of Atg7, but not Atg5, another autophagy-essential gene, in the hematopoietic system re...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104176
更新日期:2020-11-24 00:00:00
abstract::Neural stem cell (NSC) transplantation has prevailed as a promising protective strategy for cardiac arrest (CA)-induced brain damage. Surprisingly, the poor survival of neuronal cells in severe hypoxic condition restricts the utilization of this cell-based therapy. Extracellular vesicles (EVs) transfer microRNAs (miRN...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103920
更新日期:2021-01-12 00:00:00
abstract::Amine neurotransmitters, such as dopamine, serotonin, and noradrenaline, play important roles in the modulation of behaviors and metabolism of animals. InC. elegans, it has been shown that serotonin and octopamine, an invertebrate equivalent of noradrenaline, also regulate lifespan through a mechanism related to food ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100097
更新日期:2009-10-21 00:00:00
abstract::Detecting low-abundance mutations is very important for cancer diagnosis and treatment. Here we describe an improved targeted sequencing analysis that dramatically increases sequencing depth. Seven colorectal cancer (CRC) patients and seven healthy adults were enrolled in this study. We examined genetic mutations in t...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102657
更新日期:2020-01-12 00:00:00
abstract::Obesity contributes to the incidence of various tumors, including colon cancer. However, the impact of obesity on patients' survival and related mechanisms remains unclear. Multi-omics data of 227 cases of colon cancer patients combined with clinical characteristics data were acquired from The Cancer Genome Atlas (TCG...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103972
更新日期:2020-11-10 00:00:00
abstract::Chondroitin sulfate (CS) proteoglycans in perineuronal nets (PNNs) from the central nervous system (CNS) are involved in the control of plasticity and memory. Removing PNNs reactivates plasticity and restores memory in models of Alzheimer's disease and ageing. Their actions depend on the glycosaminoglycan (GAG) chains...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101256
更新日期:2017-06-28 00:00:00
abstract::The aim of this study was to investigate the expression level of Ras homolog gene family, member A (RhoA) in patients with hepatocellular carcinoma (HCC) and to investigate the prognostic and diagnostic value of RhoA. Data mining from various data bases and wet experiments on samples from Peking Union Medical College ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102110
更新日期:2019-07-22 00:00:00
abstract::As the outbreak of coronavirus disease 2019 (COVID-19) progresses, prognostic markers for early identification of high-risk individuals are an urgent medical need. Italy has one of the highest numbers of SARS-CoV-2-related deaths and one of the highest mortality rates. Worldwide, a more severe course of COVID-19 is as...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103415
更新日期:2020-06-05 00:00:00
abstract::To assist in the study of cellular aging, we established a new method of enriching physiologically aged bone marrow stromal cells (BMSCs) in animals of any age using a Percoll density gradient centrifugation technique. BMSCs from mice 2 months of age were isolated, and their cellular age determined (over 80% Scal-1+ C...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103569
更新日期:2020-07-25 00:00:00
abstract::Many studies have investigated how aging decreases human strength and endurance. However, understanding the effect of aging on human motor ability requires more than knowledge of the separate temporal profile of individual motor function because the structure of human motor ability is multi-dimensional. We address the...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102126
更新日期:2019-07-30 00:00:00
abstract::Senescent cells accumulate in various tissues and organs with aging altering surrounding tissue due to an active secretome, and at least in mice their elimination extends healthy lifespan and ameliorates several chronic diseases. Whether all cell types senesce, including post-mitotic cells, has been poorly described m...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102181
更新日期:2019-08-30 00:00:00
abstract::The mechanism underlying the association between age and depletion of the human ovarian follicle reserves remains uncertain. Many identified that impaired DNA polymerase β (Pol β)-mediated DNA base-excision repair (BER) drives to mouse oocyte aging. With aging, DNA lesions accumulate in primordial follicles. However, ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104123
更新日期:2020-11-20 00:00:00
abstract::Calorie restriction (CR) inhibits inflammation and slows aging in many animal species, but in rodents housed in pathogen-free facilities, CR impairs immunity against certain pathogens. However, little is known about the effects of long-term moderate CR on immune function in humans. In this multi-center, randomized cli...
journal_title:Aging
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.18632/aging.100994
更新日期:2016-07-01 00:00:00
abstract::Despite improvements in surgical procedures and comprehensive therapies, pancreatic cancer remains one of the most aggressive and deadly human malignancies. It is therefore necessary to determine which cellular mediators associate with prognosis in pancreatic cancer so as to improve the treatment of this disease. In t...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102096
更新日期:2019-07-21 00:00:00
abstract::Liver fibrosis biomarker, Type IV collagen, may function as hepatocarcinogenesis niche. However, among the six isoforms, the isoforms providing tumor microenvironment and their regulatory network are still unclarified. Based on bioinformatics analysis of hundreds of HCC transcriptome datasets from public databases, we...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102610
更新日期:2020-01-05 00:00:00
abstract::SAG is an essential RING component of the Cullin-RING ligase (CRL) E3 ubiquitin ligase complex, which regulates diverse signaling pathways and biological processes, including cell apoptosis, embryonic development, angiogenesis, and tumorigenesis. In the present study, we revealed that SAG gene expression is upregulate...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102663
更新日期:2020-01-11 00:00:00
abstract:AIMS:We identified an autosomal dominant non‐sense mutation (R225X) in exon 4 of the lamin A/C (LMNA) gene in a Chinese family spanning 3 generations with familial dilated cardiomyopathy (DCM). In present study, we aim to generate induced pluripotent stem cells derived cardiomyocytes (iPSC‐CMs) from an affected patient...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100503
更新日期:2012-11-01 00:00:00