Whole Genome Sequencing of Peruvian Klebsiella pneumoniae Identifies Novel Plasmid Vectors Bearing Carbapenem Resistance Gene NDM-1.


Background:Klebsiella pneumoniae is a bacterial pathogen with increasing rates of resistance to carbapenem antibiotics, but the population structure and genetic drivers of carbapenem-resistant K pneumoniae (CRKP) remain underexplored in developing countries. Carbapenem-resistant K pneumoniae were recently introduced into Peru but have grown rapidly in prevalence, enabling study of this pathogen as it expands into an unaffected environment. Methods:In this study, using whole genome sequencing, we show that 3 distinct lineages encompass almost all CRKP identified in the hospital where it was first reported in Peru. Results:The most prevalent lineage, ST348, has not been described outside of Europe, raising concern for global dissemination. We identified metallo- β -lactamase NDM-1 as the primary carbapenem resistance effector, which was harbored on a novel vector resulting from recombination between 2 different plasmids, pKP1-NDM-1 and pMS7884A. Conclusions:This study is the first of its kind performed in Peru, and it furthers our understanding of the landscape of CRKP infections in Latin America.


Open Forum Infect Dis


Roach D,Waalkes A,Abanto J,Zunt J,Cucho C,Soria J,Salipante SJ




Has Abstract


2020-07-02 00:00:00












  • Absence of HBV Reactivation in Patients With Resolved HBV Infection Following DAA Therapy for Hepatitis C: A 1-Year Follow-up Study.

    abstract:Background:Patients with chronic hepatitis C virus (HCV) infection and active or previous hepatitis B virus (HBV) are at risk of HBV reactivation (HBV-R) during direct-acting antiviral (DAA) therapy. Recent reports suggest that HBV-R may even occur several months after completion of DAA therapy. The aim of this study w...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Mücke MM,Mücke VT,Peiffer KH,Sarrazin C,Zeuzem S,Berger A,Vermehren J

    更新日期:2018-12-15 00:00:00

  • Risk Factors for Indeterminate Outcome on Interferon Gamma Release Assay in Non-US-Born Persons Screened for Latent Tuberculosis Infection.

    abstract:Background:Non-US-born individuals account for the majority of active tuberculosis (TB) in the United States. Interferon gamma release assay (IGRA) is the preferred diagnostic test for latent TB but can produce an indeterminate result. We investigated the prevalence and predictors of an indeterminate IGRA (IND-IGRA) in...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Sharninghausen JC,Shapiro AE,Koelle DM,Kim HN

    更新日期:2018-07-27 00:00:00

  • Continuing Decline in Varicella Incidence After the 2-Dose Vaccination Recommendation-Connecticut, 2009-2014.

    abstract::Background.  Varicella is a highly contagious vaccine-preventable illness. In 1996, the Advisory Committee for Immunization Practices recommended 1 dose of vaccine for children, and in 2006 it recommended 2 doses; Connecticut required 1 dose for school entry in 2000 and 2 doses for school entry starting in 2011. Conne...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Mullins J,Kudish K,Sosa L,Hadler J

    更新日期:2015-10-09 00:00:00

  • Multidrug Resistant Pseudomonas aeruginosa Causing Prosthetic Valve Endocarditis: A Genetic-Based Chronicle of Evolving Antibiotic Resistance.

    abstract:: Background.  Successful treatment of infections caused by multidrug-resistant (MDR) Pseudomonas aeruginosa is thwarted by the emergence of antibiotic resistance and biofilm formation on prosthetic devices. Our aims were to decipher the molecular basis of resistance in a un...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Domitrovic TN,Hujer AM,Perez F,Marshall SH,Hujer KM,Woc-Colburn LE,Parta M,Bonomo RA

    更新日期:2016-10-21 00:00:00


    abstract::[This corrects the article DOI: 10.1093/ofid/ofv111.]. ...

    journal_title:Open forum infectious diseases

    pub_type: 已发布勘误



    更新日期:2015-09-04 00:00:00

  • CFTR Heterozygotes Are at Increased Risk of Respiratory Infections: A Population-Based Study.

    abstract:Background:Patients heterozygous for mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene may be more susceptible to respiratory infections than the general population. Methods :We conducted a retrospective case-control study using health insurance claims. We identified patients as either h...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Polgreen PM,Brown GD,Hornick DB,Ahmad F,London B,Stoltz DA,Comellas AP

    更新日期:2018-11-01 00:00:00

  • Soluble Tumor Necrosis Factor-α Receptor 2 in Urine Is a Potential Biomarker for Noninvasive Diagnosis of Malaria During Pregnancy.

    abstract::Background.  During pregnancy, the placenta is inaccessible for diagnosis of placental malaria (PM), but soluble tumor necrosis factor-α receptors (sTNFR) are elevated in the plasma of women with PM. Methods.  In this study, sTNFR-1 and sTNFR-2 were quantified in urine of pregnant and nonpregnant Cameroonian women who...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Tassi Yunga S,Thévenon AD,Leke RG,Taylor DW

    更新日期:2016-04-26 00:00:00

  • I-FABP Is Higher in People With Chronic HIV Than Elite Controllers, Related to Sugar and Fatty Acid Intake and Inversely Related to Body Fat in People With HIV.

    abstract:Background:Intestinal fatty acid binding protein (I-FABP) has been shown to be a marker of intestinal damage among people living with HIV. We hypothesized that I-FABP would be increased in chronically HIV-infected patents more than elite controllers and would relate to specific nutrient intake and body composition. Me...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Cheru LT,Park EA,Saylor CF,Burdo TH,Fitch KV,Looby S,Weiner J,Robinson JA,Hubbard J,Torriani M,Lo J

    更新日期:2018-11-05 00:00:00

  • Evaluation of Real-time PCR for Diagnosis of Post-Kala-azar Dermal Leishmaniasis in Endemic Foci of Bangladesh.

    abstract:Background:Post-kala-azar dermal leishmaniasis (PKDL) is a sequel to visceral leishmaniasis (VL), which is found in VL-endemic countries including Bangladesh. Because of these enigmatic cases, the success of the National Kala-azar Elimination Program is under threat. To date, diagnostic methods for PKDL cases in endemi...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Ghosh P,Hasnain MG,Hossain F,Khan MAA,Chowdhury R,Faisal K,Mural MA,Baker J,Nath R,Ghosh D,Maruf S,Shomik MS,Haque R,Matlashewski G,Hamano S,Duthie MS,Mondal D

    更新日期:2018-09-15 00:00:00

  • Splenic Infarction: An Under-recognized Complication of Infectious Mononucleosis?

    abstract::Splenic infarction is a rare complication of infectious mononucleosis. We describe 3 cases of splenic infarction attributed to infectious mononucleosis that we encountered within a 2-month period. We underscore the awareness of this potential complication of infectious mononucleosis and discuss the differential diagno...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Li Y,George A,Arnaout S,Wang JP,Abraham GM

    更新日期:2018-02-17 00:00:00

  • Care Continuum and Postdischarge Outcomes Among HIV-Infected Adults Admitted to the Hospital in Zambia.

    abstract:Background:We characterized the extent of antiretroviral therapy (ART) experience and postdischarge mortality among hospitalized HIV-infected adults in Zambia. Methods:At a central hospital with an opt-out HIV testing program, we enrolled HIV-infected adults (18+ years) admitted to internal medicine using a population...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Haachambwa L,Kandiwo N,Zulu PM,Rutagwera D,Geng E,Holmes CB,Sinkala E,Claassen CW,Mugavero MJ,Wa Mwanza M,Turan JM,Vinikoor MJ

    更新日期:2019-07-16 00:00:00

  • Successful Fecal Microbiota Transplantation in a Patient Suffering From Irritable Bowel Syndrome and Recurrent Urinary Tract Infections.

    abstract:Background:Irritable bowel syndrome (IBS) is a chronic and debilitating functional gastrointestinal disorder affecting 9%-23% of the population across the world. The relative efficacy of fecal microbiota transplantation (FMT) on IBS symptoms was demonstrated in a double-blind, randomized study. Methods:We describe the...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Hocquart M,Pham T,Kuete E,Tomei E,Lagier JC,Raoult D

    更新日期:2019-10-14 00:00:00

  • Comparative Outcomes of Adults Hospitalized With Seasonal Influenza A or B Virus Infection: Application of the 7-Category Ordinal Scale.

    abstract:Background:The objective of this study was to investigate the difference in disease severity between influenza A and B among hospitalized adults using a novel ordinal scale and existing clinical outcome end points. Methods:A prospective, observational study was conducted over the 2016-2018 influenza seasons in a centr...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Wang Y,Fan G,Horby P,Hayden F,Li Q,Wu Q,Zou X,Li H,Zhan Q,Wang C,Cao B,CAP-China Network.

    更新日期:2019-02-15 00:00:00

  • Sulfamethoxazole-Trimethoprim (Cotrimoxazole) for Skin and Soft Tissue Infections Including Impetigo, Cellulitis, and Abscess.

    abstract::Skin and soft tissue infections (SSTI) affect millions of people globally, which represents a significant burden on ambulatory care and hospital settings. The role of sulfamethoxazole-trimethoprim (SXT) in SSTI treatment, particularly when group A Streptococcus (GAS) is involved, is controversial. We conducted a syste...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章,评审


    authors: Bowen AC,Carapetis JR,Currie BJ,Fowler V Jr,Chambers HF,Tong SYC

    更新日期:2017-11-02 00:00:00

  • Safety and Efficacy of Ombitasvir, Paritaprevir With Ritonavir ± Dasabuvir With or Without Ribavirin in Patients With Human Immunodeficiency Virus-1 and Hepatitis C Virus Genotype 1 or Genotype 4 Coinfection: TURQUOISE-I Part 2.

    abstract:BACKGROUND:Ombitasvir, paritaprevir with ritonavir, and dasabuvir (OBV/PTV/r ± DSV) ±ribavirin (RBV) are approved to treat hepatitis C virus (HCV) genotype 1 and 4 infection. Here, we investigate the safety and efficacy of OBV/PTV/r + DSV ±RBV for HCV genotype 1, and OBV/PTV/r + RBV for HCV genotype 4, in human immunod...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Rockstroh JK,Orkin C,Viani RM,Wyles D,Luetkemeyer AF,Lazzarin A,Soto-Malave R,Nelson MR,Bhagani SR,Klinker HHF,Rizzardini G,Girard PM,Tural C,Shulman NS,Mobashery N,Hu YB,Fredrick LM,Pilot-Matias T,Trinh R,Gane E

    更新日期:2017-07-22 00:00:00

  • Faces of Resistance: Using Real-World Patients and Their Advocates to Teach Medical Students About Antimicrobial Stewardship.

    abstract::We engaged medical students with antimicrobial stewardship (AS) and antimicrobial resistance (AMR) through patient stories and a panel on AMR advocacy with experts from the Centers for Disease Control and Prevention and the Infectious Diseases Society of America. Students were surveyed on their perceptions about AS an...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Nori P,Cowman K,Jezek A,Nosanchuk JD,Slosar-Cheah M,Sarwar U,Bartash R,Ostrowsky B

    更新日期:2019-11-13 00:00:00

  • Syphilis Reactivates Latent Epstein-Barr Virus Reservoir via Toll-Like Receptor 2 and B-Cell Receptor Activation.

    abstract::Latent Epstein-Barr virus (EBV) infection can clinically reactivate in immunosuppressed individuals causing lymphoproliferative disease and rarely hepatitis. In this study, we provide in vivo and in vitro evidence that Treponema pallidum infection can cause EBV reactivation with hepatitis in an immunocompetent patient...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Hirsiger JR,Fuchs PS,Häusermann P,Müller-Durovic B,Daikeler T,Recher M,Hirsch HH,Terracciano L,Berger CT

    更新日期:2019-07-03 00:00:00

  • Outcomes of Older Adults With Sepsis at Admission to an Intensive Care Unit.

    abstract::Background.  Sepsis is a major cause of morbidity and mortality among older adults. The main goals of this study were to assess the association of sepsis at intensive care unit (ICU) admission with mortality and to identify predictors associated with increased mortality in older adults. Methods.  We conducted a prospe...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Rowe T,Araujo KL,Van Ness PH,Pisani MA,Juthani-Mehta M

    更新日期:2016-01-21 00:00:00

  • Antimicrobial Susceptibility Trends Observed in Urinary Pathogens Obtained From New York State.

    abstract::International guidelines recommend using local susceptibility data to direct empiric therapy for acute uncomplicated cystitis. We evaluated outpatient urinary isolate susceptibility trends in New York State. Nitrofurantoin had the lowest resistance prevalence whereas trimethoprim-sulfamethoxazole and fluoroquinolones ...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Rank EL,Lodise T,Avery L,Bankert E,Dobson E,Dumyati G,Hassett S,Keller M,Pearsall M,Lubowski T,Carreno JJ

    更新日期:2018-11-16 00:00:00

  • Immunogenicity and Reactogenicity of a Reduced Schedule of a 4-component Capsular Group B Meningococcal Vaccine: A Randomized Controlled Trial in Infants.

    abstract:Background:The 4-component capsular group B meningococcal vaccine (4CMenB) was licensed as a 4-dose infant schedule but introduced into the United Kingdom as 3 doses at 2, 4, and 12 months of age. We describe the immunogenicity and reactogenicity of the 2 + 1 schedule in infants. Methods:Infants were randomized to rec...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Valente Pinto M,O'Connor D,Galal U,Clutterbuck EA,Robinson H,Plested E,Bibi S,Camara Pellisso S,Hughes H,Kerridge S,Mujadidi YF,Findlow H,Borrow R,Snape MD,Pollard AJ

    更新日期:2020-04-29 00:00:00

  • Risk Factors for Herpes Zoster Among Adults.

    abstract::Background.  The causes of varicella-zoster virus reactivation and herpes zoster (HZ) are largely unknown. We assessed potential risk factors for HZ, the data for which cannot be obtained from the medical sector. Methods.  We conducted a matched case-control study. We established active surveillance in Olmsted County,...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Marin M,Harpaz R,Zhang J,Wollan PC,Bialek SR,Yawn BP

    更新日期:2016-06-11 00:00:00

  • Remdesivir Use Compared With Supportive Care in Hospitalized Patients With Severe COVID-19: A Single-Center Experience.

    abstract:Background:The US Food and Drug Administration issued an Emergency Use Authorization for remdesivir use in patients with severe COVID-19. Methods:We utilized data from 2 quaternary acute care hospitals. The outcomes of interest were the impact of remdesivir on in-hospital death by day 28 and time to recovery, clinical...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Kalligeros M,Tashima KT,Mylona EK,Rybak N,Flanigan TP,Farmakiotis D,Beckwith CG,Sanchez M,Neill M,Johnson JE,Garland JM,Aung S,Byrd KM,O'Brien T,Pandita A,Aridi J,Macias Gil R,Larkin J,Shehadeh F,Mylonakis E

    更新日期:2020-08-06 00:00:00

  • Trends in Hospitalizations Related to Invasive Aspergillosis and Mucormycosis in the United States, 2000-2013.

    abstract:BACKGROUND:Invasive aspergillosis (IA) and mucormycosis contribute to substantial mortality, especially among immunocompromised persons, including those with hematopoietic stem cell transplant (HSCT), hematologic malignancy (HM), and solid organ transplant (SOT). METHODS:Using International Classification of Diseases,...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Vallabhaneni S,Benedict K,Derado G,Mody RK

    更新日期:2017-01-13 00:00:00

  • Hourly Effect of Pretreatment With IV Antibiotics on Blood Culture Positivity Rate in Emergency Department Patients.

    abstract::Although it is intuitive that antibiotics administered before obtaining a blood culture would reduce the likelihood of obtaining a positive culture, it is not clear exactly how rapidly and to what extent blood becomes sterile after administration of intravenous (IV) antibiotics. Using a large data set of patients admi...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Rand KH,Beal SG,Rivera K,Allen B,Payton T,Lipori GP

    更新日期:2019-05-20 00:00:00

  • Endothelial activation and repair during hantavirus infection: association with disease outcome.

    abstract:BACKGROUND:Endothelial activation and dysfunction play a central role in the pathogenesis of sepsis and viral hemorrhagic fevers. Hantaviral disease is a viral hemorrhagic fever and is characterized by capillary dysfunction, although the underlying mechanisms for hantaviral disease are not fully elucidated. METHODS:Th...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Connolly-Andersen AM,Thunberg T,Ahlm C

    更新日期:2014-05-29 00:00:00

  • Switch to Raltegravir From Protease Inhibitor or Nonnucleoside Reverse-Transcriptase Inhibitor Does not Reduce Visceral Fat In Human Immunodeficiency Virus-Infected Women With Central Adiposity.

    abstract::Human immunodeficiency virus-infected women with central adiposity switched to raltegravir-based antiretroviral therapy immediately or after 24 weeks. No statistically significant changes in computed tomography-quantified visceral adipose tissue (VAT) or subcutaneous fat were observed, although 48 weeks of raltegravir...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Lake JE,McComsey GA,Hulgan T,Wanke CA,Mangili A,Walmsley SL,Currier JS

    更新日期:2015-04-26 00:00:00

  • Transfusion-Transmitted Malaria: A Systematic Review and Meta-analysis.

    abstract:Background:Malaria transmission through blood transfusion is an accidental but preventable cause of malaria infection and is increasingly becoming a matter of concern for blood transfusion services. This systematic review was conducted to provide a summary of evidence about the prevalence of Plasmodium infection in asy...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Ahmadpour E,Foroutan-Rad M,Majidiani H,Moghaddam SM,Hatam-Nahavandi K,Hosseini SA,Rahimi MT,Barac A,Rubino S,Zarean M,Mathioudakis AG,Cevik M

    更新日期:2019-06-11 00:00:00

  • Role of HCV Viremia in Corroborated HCV Transmission Events Within Young Adult Injecting Partnerships.

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    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Hahn JA,Tully DC,Evans JL,Morris MD,Briceno A,Bean DJ,Allen TM,Page K

    更新日期:2019-04-25 00:00:00

  • Learning Experiences Within Infectious Diseases Pharmacy Residency Programs in the United States: A Cross-Sectional Survey.

    abstract:Background:Pharmacists with residency training in infectious diseases (ID) optimize antimicrobial therapy outcomes in patients and support antimicrobial stewardship (AS) programs. Although most ID residencies are accredited and assessed by certain standards, the degree to which these programs are similar is not known. ...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Cho JC,Crotty MP,Kufel WD,Chahine EB,Sofjan AK,Gallagher JC,Estrada SJ

    更新日期:2019-09-06 00:00:00

  • Human Immunodeficiency Virus-1 Sequence Changes and Drug Resistance Mutation Among Virologic Failures of Lopinavir/Ritonavir Monotherapy: AIDS Clinical Trials Group Protocol A5230.

    abstract:: Background.  The mechanism of virologic failure (VF) of lopinavir/ritonavir (LPV/r) monotherapy is not well understood. We assessed sequence changes in human immunodeficiency virus-1 reverse-transcriptase (RT) and protease (PR) regions. Methods. ...

    journal_title:Open forum infectious diseases

    pub_type: 杂志文章


    authors: Vardhanabhuti S,Katzenstein D,Bartlett J,Kumarasamy N,Wallis CL

    更新日期:2016-08-24 00:00:00