Activation of M1 Macrophages in Response to Recombinant TB Vaccines With Enhanced Antimycobacterial Activity.


:Pulmonary tuberculosis (TB) is a difficult-to-eliminate disease. Although the Bacille Calmette-Guérin (BCG) vaccine against Mycobacterium tuberculosis (MTB) has been available for decades, its efficacy is variable and has lessened over time. Furthermore, the BCG vaccine no longer protects against newly emerged Beijing strains which are responsible for many current infections in adults. Development of a novel vaccine is urgently needed. In this study, we first tested the efficacy of our recombinant BCG vaccines rBCG1 and rBCG2, compared to parental BCG, against MTB strain H37Ra in mice. Both the bacterial load and the level of lymphocyte infiltration decreased dramatically in the three groups treated with vaccine, especially rBCG1 and rBCG2. Furthermore, the Th1 and Th17 responses increased and macrophage numbers rose in the vaccination groups. Th1-mediated production of cytokines TNF-α, IFN-γ, and MCP-1 as well as M1-polarized cells all increased in lung tissue of the rBCG1 and rBCG2 groups. Clodronate-induced depletion of macrophages reduced the level of protection. Based on these results, we conclude that rBCG vaccines induce a significant increase in the number of M1 macrophages, which augments their potential as TB vaccine candidates.


Front Immunol


Frontiers in immunology


Yang SJ,Chen YY,Hsu CH,Hsu CW,Chang CY,Chang JR,Dou HY




Has Abstract


2020-06-23 00:00:00








  • Targeting the Human T-Cell Inducible COStimulator Molecule with a Monoclonal Antibody Prevents Graft-vs-Host Disease and Preserves Graft vs Leukemia in a Xenograft Murine Model.

    abstract:BACKGROUND:Graft-vs-host disease (GVHD) is a major complication of allogenic bone marrow transplantation (BMT). Targeting costimulatory molecules with antagonist antibodies could dampen the excessive immune response that occurs, while preserving the beneficial graft vs leukemia (GVL) of the allogeneic response. Previou...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Burlion A,Brunel S,Petit NY,Olive D,Marodon G

    更新日期:2017-06-30 00:00:00

  • Streptococcal Enzymes as Precision Tools Against Pathogenic IgG Autoantibodies in Small Vessel Vasculitis.

    abstract::In primary systemic small vessel vasculitis autoantibodies are common and seem to play an important role in the pathogenesis. Autoantibodies in vasculitis are preferentially directed against components of the immune system or directly against components of the vessel wall. Plasmapheresis is often applied in emergency ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Segelmark M,Björck L

    更新日期:2019-09-20 00:00:00

  • Corrigendum: The Effects of Framework Mutations at the Variable Domain Interface on Antibody Affinity Maturation in an HIV-1 Broadly Neutralizing Antibody Lineage.

    abstract::[This corrects the article DOI: 10.3389/fimmu.2020.01529.]. ...

    journal_title:Frontiers in immunology

    pub_type: 已发布勘误


    authors: Zhou JO,Zaidi HA,Ton T,Fera D

    更新日期:2020-11-18 00:00:00

  • Novel Genome-Editing Tools to Model and Correct Primary Immunodeficiencies.

    abstract::Severe combined immunodeficiency (SCID) and other severe non-SCID primary immunodeficiencies (non-SCID PID) can be treated by allogeneic hematopoietic stem cell (HSC) transplantation, but when histocompatibility leukocyte antigen-matched donors are lacking, this can be a high-risk procedure. Correcting the patient's o...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Ott de Bruin LM,Volpi S,Musunuru K

    更新日期:2015-05-21 00:00:00

  • Negative Effect of Age, but Not of Latent Cytomegalovirus Infection on the Antibody Response to a Novel Influenza Vaccine Strain in Healthy Adults.

    abstract::Older adults are more vulnerable to influenza virus infection and at higher risk for severe complications and influenza-related death compared to younger adults. Unfortunately, influenza vaccine responses tend to be impaired in older adults due to aging of the immune system (immunosenescence). Latent infection with cy...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: van den Berg SPH,Wong A,Hendriks M,Jacobi RHJ,van Baarle D,van Beek J

    更新日期:2018-01-29 00:00:00

  • Natural-Killer-Derived Extracellular Vesicles: Immune Sensors and Interactors.

    abstract::Natural killer (NK) cells contribute to immunosurveillance and first-line defense in the control of tumor growth and metastasis diffusion. NK-cell-derived extracellular vesicles (NKEVs) are constitutively secreted and biologically active. They reflect the protein and genetic repertoire of originating cells, and exert ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Federici C,Shahaj E,Cecchetti S,Camerini S,Casella M,Iessi E,Camisaschi C,Paolino G,Calvieri S,Ferro S,Cova A,Squarcina P,Bertuccini L,Iosi F,Huber V,Lugini L

    更新日期:2020-03-13 00:00:00

  • A Review of the Progress and Challenges of Developing a Vaccine for COVID-19.

    abstract::A novel coronavirus, which has been designated as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was first detected in December 2019 in Wuhan China and causes the highly infectious disease referred to as COVID-19. COVID-19 has now spread worldwide to become a global pandemic affecting over 24 million pe...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Sharma O,Sultan AA,Ding H,Triggle CR

    更新日期:2020-10-14 00:00:00

  • Tuftsin Combines With Remyelinating Therapy and Improves Outcomes in Models of CNS Demyelinating Disease.

    abstract::Though promoting remyelination in multiple sclerosis (MS) has emerged as a promising therapeutic strategy, it does not address inflammatory signals that continue to induce neuronal damage and inhibit effectiveness of repair mechanisms. Our lab has previously characterized the immunomodulatory tetrapeptide, tuftsin, wh...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Thompson KK,Nissen JC,Pretory A,Tsirka SE

    更新日期:2018-11-28 00:00:00

  • Deletion of chr7p22 and chr15q11: Two Familial Cases of Immune Deficiency: Extending the Phenotype Toward Dysimmunity.

    abstract::Background: We report here two new familial cases of associated del15q11 and del7p22, with the latter underlining the clinical variability of this deletion. Two siblings patients presented a similar familial imbalanced translocation, originating from a balanced maternal translocation, with deletions of 7p22 and of 15q...

    journal_title:Frontiers in immunology



    authors: Sloboda N,Sorlin A,Valduga M,Beri-Dexheimer M,Bilbault C,Fouyssac F,Becker A,Lambert L,Bonnet C,Leheup B

    更新日期:2019-08-16 00:00:00

  • Prefusion RSV F Immunization Elicits Th2-Mediated Lung Pathology in Mice When Formulated With a Th2 (but Not a Th1/Th2-Balanced) Adjuvant Despite Complete Viral Protection.

    abstract::Respiratory syncytial virus (RSV) remains the most common cause of lower respiratory tract infections in children worldwide. Development of a vaccine has been hindered by the risk of developing enhanced respiratory disease (ERD) upon natural exposure to the virus. Generation of higher quality neutralizing antibodies w...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Eichinger KM,Kosanovich JL,Gidwani SV,Zomback A,Lipp MA,Perkins TN,Oury TD,Petrovsky N,Marshall CP,Yondola MA,Empey KM

    更新日期:2020-07-29 00:00:00

  • A Functional Genetic Variant at the C-Reactive Protein Promoter (rs3091244) Is Not Associated With Cancer Risk in a Chinese Population.

    abstract::Background: The association of genetically elevated levels of circulating C-reactive protein (CRP) with cancer risk has been extensively investigated in European populations; however, there are conflicting conclusions. The tri-allelic rs3091244 is a functionally validated genetic variant, and its allelic frequencies d...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Wang MY,Zhou HH,Zhang CM,Su HX,Li SL,Ji SR,Liu E,Wu Y

    更新日期:2020-05-14 00:00:00

  • IMPre: An Accurate and Efficient Software for Prediction of T- and B-Cell Receptor Germline Genes and Alleles from Rearranged Repertoire Data.

    abstract::Large-scale study of the properties of T-cell receptor (TCR) and B-cell receptor (BCR) repertoires through next-generation sequencing is providing excellent insights into the understanding of adaptive immune responses. Variable(Diversity)Joining [V(D)J] germline genes and alleles must be characterized in detail to fac...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Zhang W,Wang IM,Wang C,Lin L,Chai X,Wu J,Bett AJ,Dhanasekaran G,Casimiro DR,Liu X

    更新日期:2016-11-04 00:00:00

  • IL-10 Protects Mice From the Lung Infection of Acinetobacter baumannii and Contributes to Bacterial Clearance by Regulating STAT3-Mediated MARCO Expression in Macrophages.

    abstract::Interleukin-10 plays important, yet contrasting, roles in host protection against bacterial infections and in the septic response. To determine the role of IL-10 in the host defense against Acinetobacter baumannii infection, wild-type (WT) and IL-10-deficient mice were infected intranasally with the bacteria. IL-10-de...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Kang MJ,Jang AR,Park JY,Ahn JH,Lee TS,Kim DY,Lee MS,Hwang S,Jeong YJ,Park JH

    更新日期:2020-02-21 00:00:00

  • Imprinting and Editing of the Human CD4 T Cell Response to Influenza Virus.

    abstract::Immunity to influenza is unique among pathogens, in that immune memory is established both via intermittent lung localized infections with highly variable influenza virus strains and by intramuscular vaccinations with inactivated protein-based vaccines. Studies in the past decades have suggested that the B cell respon...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Nelson SA,Sant AJ

    更新日期:2019-05-07 00:00:00

  • Extracellular Acidification Inhibits the ROS-Dependent Formation of Neutrophil Extracellular Traps.

    abstract::The inflammatory microenvironment is commonly characterized by extracellular acidosis (pH < 7.35). Sensitivity to pH, CO2 or bicarbonate concentrations allows neutrophils to react to changes in their environment and to detect inflamed areas in the tissue. One important antimicrobial effector mechanism is the productio...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Behnen M,Möller S,Brozek A,Klinger M,Laskay T

    更新日期:2017-02-28 00:00:00

  • Towards a liquid self: how time, geography, and life experiences reshape the biological identity.

    abstract::The conceptualization of immunological self is amongst the most important theories of modern biology, representing a sort of theoretical guideline for experimental immunologists, in order to understand how host constituents are ignored by the immune system (IS). A consistent advancement in this field has been represen...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Grignolio A,Mishto M,Faria AM,Garagnani P,Franceschi C,Tieri P

    更新日期:2014-04-09 00:00:00

  • Indoleamine 2,3-Dioxygenase and Tolerance: Where Are We Now?

    abstract::Cells expressing IDO suppress innate and adaptive immunity to promote tolerance by catabolizing the amino acid tryptophan (Trp) and other indole compounds. Interferon type I (IFN-I) and type II (IFN-II) produced at sites of inflammation or by activated immune cells are potent IDO inducers because mammalian IDO genes c...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Mellor AL,Lemos H,Huang L

    更新日期:2017-10-27 00:00:00

  • The Interaction Mechanism Between Herpes Simplex Virus 1 Glycoprotein D and Host Antiviral Protein Viperin.

    abstract::Viperin is an interferon-inducible protein that responsible for a variety of antiviral responses to different viruses. Our previous study has shown that the ribonuclease UL41 of herpes simplex virus 1 (HSV-1) can degrade the mRNA of viperin to promote HSV-1 replication. However, it is not clear whether other HSV-1 enc...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Li M,Liao Z,Xu Z,Zou X,Wang Y,Peng H,Li Y,Ou X,Deng Y,Guo Y,Gan W,Peng T,Chen D,Cai M

    更新日期:2019-12-11 00:00:00

  • Dendritic Cell Regulation of Graft-Vs.-Host Disease: Immunostimulation and Tolerance.

    abstract::Graft-vs.-host disease (GVHD) remains a significant cause of morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Significant progresses have been made in defining the dichotomous role of dendritic cells (DCs) in the development of GVHD. Host-derived DCs are important to elicit...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Yu H,Tian Y,Wang Y,Mineishi S,Zhang Y

    更新日期:2019-02-01 00:00:00

  • Porcine Invariant Natural Killer T Cells: Functional Profiling and Dynamics in Steady State and Viral Infections.

    abstract::Pigs are important livestock and comprehensive understanding of their immune responses in infections is critical to improve vaccines and therapies. Moreover, similarities between human and swine physiology suggest that pigs are a superior animal model for immunological studies. However, paucity of experimental tools f...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Schäfer A,Hühr J,Schwaiger T,Dorhoi A,Mettenleiter TC,Blome S,Schröder C,Blohm U

    更新日期:2019-06-18 00:00:00

  • Cell Type-Specific Regulation of Immunological Synapse Dynamics by B7 Ligand Recognition.

    abstract::B7 proteins CD80 (B7-1) and CD86 (B7-2) are expressed on most antigen-presenting cells and provide critical co-stimulatory or inhibitory input to T cells via their T-cell-expressed receptors: CD28 and CTLA-4. CD28 is expressed on effector T cells and regulatory T cells (Tregs), and CD28-dependent signals are required ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Brzostek J,Gascoigne NR,Rybakin V

    更新日期:2016-02-04 00:00:00

  • Modulation of Crustacean Innate Immune Response by Amino Acids and Their Metabolites: Inferences From Other Species.

    abstract::Aquaculture production of crustaceans (mainly shrimp and crabs) has expanded globally, but disease outbreaks and pathogenic infections have hampered production in the last two decades. As invertebrates, crustaceans lack an adaptive immune system and mainly defend and protect themselves using their innate immune system...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Huang Z,Aweya JJ,Zhu C,Tran NT,Hong Y,Li S,Yao D,Zhang Y

    更新日期:2020-11-05 00:00:00

  • Stem cell transplantation as a dynamical system: are clinical outcomes deterministic?

    abstract::Outcomes in stem cell transplantation (SCT) are modeled using probability theory. However, the clinical course following SCT appears to demonstrate many characteristics of dynamical systems, especially when outcomes are considered in the context of immune reconstitution. Dynamical systems tend to evolve over time acco...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Toor AA,Kobulnicky JD,Salman S,Roberts CH,Jameson-Lee M,Meier J,Scalora A,Sheth N,Koparde V,Serrano M,Buck GA,Clark WB,McCarty JM,Chung HM,Manjili MH,Sabo RT,Neale MC

    更新日期:2014-12-03 00:00:00

  • Influenza and Antibody-Dependent Cellular Cytotoxicity.

    abstract::Despite the availability of yearly vaccinations, influenza continues to cause seasonal, and pandemic rises in illness and death. An error prone replication mechanism results in antigenic drift and viral escape from immune pressure, and recombination results in antigenic shift that can rapidly move through populations ...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Von Holle TA,Moody MA

    更新日期:2019-06-25 00:00:00

  • A Novel Siglec-4 Derived Spacer Improves the Functionality of CAR T Cells Against Membrane-Proximal Epitopes.

    abstract::A domain that is often neglected in the assessment of chimeric antigen receptor (CAR) functionality is the extracellular spacer module. However, several studies have elucidated that membrane proximal epitopes are best targeted through CARs comprising long spacers, while short spacer CARs exhibit highest activity on di...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Schäfer D,Henze J,Pfeifer R,Schleicher A,Brauner J,Mockel-Tenbrinck N,Barth C,Gudert D,Al Rawashdeh W,Johnston ICD,Hardt O

    更新日期:2020-08-07 00:00:00

  • Piperlongumine Acts as an Immunosuppressant by Exerting Prooxidative Effects in Human T Cells Resulting in Diminished TH17 but Enhanced Treg Differentiation.

    abstract::Piperlongumine (PL), a natural small molecule derived from the Piper longum Linn plant, has received growing interest as a prooxidative drug with promising anticancer properties. Yet, the influence of PL on primary human T cells remained elusive. Knowledge of this is of crucial importance, however, since T cells in pa...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Liang J,Ziegler JD,Jahraus B,Orlik C,Blatnik R,Blank N,Niesler B,Wabnitz G,Ruppert T,Hübner K,Balta E,Samstag Y

    更新日期:2020-06-12 00:00:00

  • Role of SLC7A5 in Metabolic Reprogramming of Human Monocyte/Macrophage Immune Responses.

    abstract::Amino acids (AAs) are necessary nutrients which act not only as building blocks in protein synthesis but also in crucial anabolic cellular signaling pathways. It has been demonstrated that SLC7A5 is a critical transporter that mediates uptake of several essential amino acids in highly proliferative tumors and activate...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章


    authors: Yoon BR,Oh YJ,Kang SW,Lee EB,Lee WW

    更新日期:2018-01-25 00:00:00

  • Inhibitors of Serine Proteases in Regulating the Production and Function of Neutrophil Extracellular Traps.

    abstract::Neutrophil extracellular traps (NETs), DNA webs released into the extracellular environment by activated neutrophils, are thought to play a key role in the entrapment and eradication of microbes. However, NETs are highly cytotoxic and a likely source of autoantigens, suggesting that NET release is tightly regulated. N...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Majewski P,Majchrzak-Gorecka M,Grygier B,Skrzeczynska-Moncznik J,Osiecka O,Cichy J

    更新日期:2016-06-30 00:00:00

  • Role of Regulatory Immune Cells and Molecules in Autoimmune Bullous Dermatoses.

    abstract::Autoimmune bullous dermatoses (AIBD) include a series of typical organ-specific autoimmune diseases characterized by extensive mucocutaneous blisters. It is generally accepted to be caused by pathological autoantibodies that directly target specific adhesion components of the skin or the adjacent mucous membranes. Bot...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Cao T,Shao S,Fang H,Li B,Wang G

    更新日期:2019-08-02 00:00:00

  • T Follicular Helper Cells and B Cell Dysfunction in Aging and HIV-1 Infection.

    abstract::T follicular helper (Tfh) cells are a subset of CD4 T cells that provide critical signals to antigen-primed B cells in germinal centers to undergo proliferation, isotype switching, and somatic hypermutation to generate long-lived plasma cells and memory B cells during an immune response. The quantity and quality of Tf...

    journal_title:Frontiers in immunology

    pub_type: 杂志文章,评审


    authors: Pallikkuth S,de Armas L,Rinaldi S,Pahwa S

    更新日期:2017-10-23 00:00:00