Brain Endothelial Cells Regulate Glucagon-Like Peptide 1 Entry Into the Brain via a Receptor-Mediated Process.

Abstract:

:Glucagon-like peptide 1 (GLP-1) in addition to regulating glucose-dependent insulin and glucagon secretion exerts anorexic and neuroprotective effects. While brain-derived GLP-1 may participate in these central actions, evidence suggests that peripherally derived GLP-1 plays an important role and GLP-1 analogs are known to cross the blood brain barrier. To define the role of brain microvascular endothelial cells in GLP-1 entry into the brain, we infused labeled GLP-1 or exendin-4 into rats intravenously and examined their appearance and protein kinase A activities in various brain regions. We also studied the role of endothelial cell GLP-1 receptor and its signaling in endothelial cell uptake and transport of GLP-1. Systemically infused labeled GLP-1 or exendin-4 appeared rapidly in various brain regions and this was associated with increased protein kinase A activity in these brain regions. Pretreatment with GLP-1 receptor antagonist reduced labeled GLP-1 or exendin-4 enrichment in the brain. Sub-diaphragmatic vagus nerve resection did not alter GLP-1-mediated increases in protein kinase A activity in the brain. Rat brain microvascular endothelial cells rapidly took up labeled GLP-1 and this was blunted by either GLP-1 receptor antagonism or protein kinase A inhibition but enhanced through adenylyl cyclase activation. Using an artificially assembled blood brain barrier consisting of endothelial and astrocyte layers, we found that labeled GLP-1 time-dependently crossed the barrier and the presence of GLP-1 receptor antagonist blunted this transit. We conclude that GLP-1 crosses the blood brain barrier through active trans-endothelial transport which requires GLP-1 receptor binding and activation.

journal_name

Front Physiol

journal_title

Frontiers in physiology

authors

Fu Z,Gong L,Liu J,Wu J,Barrett EJ,Aylor KW,Liu Z

doi

10.3389/fphys.2020.00555

subject

Has Abstract

pub_date

2020-05-29 00:00:00

pages

555

issn

1664-042X

journal_volume

11

pub_type

杂志文章
  • Corrigendum: Two Consecutive Days of Extreme Conditioning Program Training Affects Pro and Anti-inflammatory Cytokines and Osteoprotegerin without Impairments in Muscle Power.

    abstract::[This corrects the article DOI: 10.3389/fphys.2016.00260.]. ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,已发布勘误

    doi:10.3389/fphys.2018.00771

    authors: Tibana RA,de Almeida LM,Frade de Sousa NM,Nascimento DDC,Neto IVS,de Almeida JA,de Souza VC,Lopes MFTPL,Nobrega OT,Vieira DCL,Navalta JW,Prestes J

    更新日期:2018-07-16 00:00:00

  • Wearable Inertial Measurement Units for Assessing Gait in Real-World Environments.

    abstract:Background:Walking patterns can provide important indications of a person's health status and be beneficial in the early diagnosis of individuals with a potential walking disorder. For appropriate gait analysis, it is critical that natural functional walking characteristics are captured, rather than those experienced i...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00090

    authors: Renggli D,Graf C,Tachatos N,Singh N,Meboldt M,Taylor WR,Stieglitz L,Schmid Daners M

    更新日期:2020-02-20 00:00:00

  • Contribution of Extracellular Vesicles in Rebuilding Injured Muscles.

    abstract::Skeletal myofibers are injured due to mechanical stresses experienced during physical activity, or due to myofiber fragility caused by genetic diseases. The injured myofiber needs to be repaired or regenerated to restore the loss in muscle tissue function. Myofiber repair and regeneration requires coordinated action o...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2019.00828

    authors: Bittel DC,Jaiswal JK

    更新日期:2019-07-18 00:00:00

  • Contribution of Post-translational Phosphorylation to Sarcomere-Linked Cardiomyopathy Phenotypes.

    abstract::Secondary shifts develop in post-translational phosphorylation of sarcomeric proteins in multiple animal models of inherited cardiomyopathy. These signaling alterations together with the primary mutation are predicted to contribute to the overall cardiac phenotype. As a result, identification and integration of post-t...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2016.00407

    authors: Westfall MV

    更新日期:2016-09-14 00:00:00

  • The blue-collar brain.

    abstract::Much effort has gone into elucidating control of the body by the brain, less so the role of the body in controlling the brain. This essay develops the idea that the brain does a great deal of work in the service of behavior that is controlled by the body, a blue-collar role compared to the white-collar control exercis...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2012.00207

    authors: Van Orden G,Hollis G,Wallot S

    更新日期:2012-06-18 00:00:00

  • Identification of Wolbachia-Responsive miRNAs in the Small Brown Planthopper, Laodelphax striatellus.

    abstract::Laodelphax striatellus is naturally infected with the Wolbachia strain wStri, which induces strong cytoplasmic incompatibility of its host. MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs that play a critical role in the regulation of gene expression at post-transcriptional level in various biologic...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.00928

    authors: Liu L,Zhang KJ,Rong X,Li YY,Liu H

    更新日期:2019-07-24 00:00:00

  • Sex-Specific Impact of Ischemic Preconditioning on Tissue Oxygenation and Maximal Concentric Force.

    abstract::Prior peripheral hypoxia induced via remote ischemic preconditioning (IPC) can improve physical performance in male athletes through improved O2 delivery and utilization. Since females may have an innate protective mechanism against ischemia-reperfusion injury, and since muscle metabolism during contraction differs be...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2016.00674

    authors: Paradis-Deschênes P,Joanisse DR,Billaut F

    更新日期:2017-01-05 00:00:00

  • Intense Physical Exercise Induces an Anti-inflammatory Change in IgG N-Glycosylation Profile.

    abstract::Exercise is known to improve many aspects of human health, including modulation of the immune system and inflammatory status. It is generally understood that exercise reduces inflammation, but there are missing links in terms of understanding the mechanisms as well as the differences between exercise modalities. N-gly...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.01522

    authors: Tijardović M,Marijančević D,Bok D,Kifer D,Lauc G,Gornik O,Keser T

    更新日期:2019-12-20 00:00:00

  • Regulatory Functions of Nilaparvata lugens GSK-3 in Energy and Chitin Metabolism.

    abstract::Glucose metabolism is a biologically important metabolic process. Glycogen synthase kinase (GSK-3) is a key enzyme located in the middle of the sugar metabolism pathway that can regulate the energy metabolism process in the body through insulin signaling. This paper mainly explores the regulatory effect of glycogen sy...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.518876

    authors: Ding YJ,Li GY,Xu CD,Wu Y,Zhou ZS,Wang SG,Li C

    更新日期:2020-11-25 00:00:00

  • Effects of Remote Ischemic Preconditioning in Patients Undergoing Off-Pump Coronary Artery Bypass Graft Surgery.

    abstract:Purpose:This study aimed to evaluate effects of remote ischemic preconditioning (RIPC) on myocardial injury in patients undergoing off-pump coronary artery bypass graft surgery (OPCABG). Methods:Sixty-five patients scheduled for the OPCABG were randomly assigned to control (n = 32) or RIPC group (n = 33). All patients...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.00495

    authors: Wang H,Lyu Y,Liao Q,Jin L,Xu L,Hu Y,Yu Y,Guo K

    更新日期:2019-04-29 00:00:00

  • Experimental Mapping of the Canine KCNJ2 and KCNJ12 Gene Structures and Functional Analysis of the Canine K(IR)2.2 ion Channel.

    abstract::For many model organisms traditionally in use for cardiac electrophysiological studies, characterization of ion channel genes is lacking. We focused here on two genes encoding the inward rectifier current, KCNJ2 and KCNJ12, in the dog heart. A combination of RT-PCR, 5'-RACE, and 3'-RACE demonstrated the status of KCNJ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2012.00009

    authors: Houtman MJ,Takanari H,Kok BG,van Eck M,Montagne DR,Vos MA,de Boer TP,van der Heyden MA

    更新日期:2012-01-30 00:00:00

  • Proton-Translocating Nicotinamide Nucleotide Transhydrogenase: A Structural Perspective.

    abstract::Nicotinamide nucleotide transhydrogenase (TH) is an enzyme complex in animal mitochondria and bacteria that utilizes the electrochemical proton gradient across membranes to drive the production of NADPH. The enzyme plays an important role in maintaining the redox balance of cells with implications in aging and a numbe...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2017.01089

    authors: Zhang Q,Padayatti PS,Leung JH

    更新日期:2017-12-19 00:00:00

  • Personalized Cardiac Computational Models: From Clinical Data to Simulation of Infarct-Related Ventricular Tachycardia.

    abstract::In the chronic stage of myocardial infarction, a significant number of patients develop life-threatening ventricular tachycardias (VT) due to the arrhythmogenic nature of the remodeled myocardium. Radiofrequency ablation (RFA) is a common procedure to isolate reentry pathways across the infarct scar that are responsib...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.00580

    authors: Lopez-Perez A,Sebastian R,Izquierdo M,Ruiz R,Bishop M,Ferrero JM

    更新日期:2019-05-15 00:00:00

  • The Comparison of Different Stretching Intensities on the Range of Motion and Muscle Stiffness of the Quadriceps Muscles.

    abstract::Muscle strain is one of the most frequent sports injuries, having the rectus femoris (RF) muscle as the reported preferred site of quadriceps muscle strain. The decrease muscle stiffness could be an effective RF muscle strain prevention. In recent studies, a high-intensity static stretching intervention decreased pass...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.628870

    authors: Nakamura M,Sato S,Murakami Y,Kiyono R,Yahata K,Sanuki F,Yoshida R,Fukaya T,Takeuchi K

    更新日期:2021-01-13 00:00:00

  • Effects of Renal Sympathetic Denervation on 24-hour Blood Pressure Variability.

    abstract:BACKGROUND:In patients with arterial hypertension, increased blood pressure (BP) variability contributes to end organ damage independently from mean levels of arterial BP. Increased BP variability has been linked to alterations in autonomic function including sympathetic overdrive. We hypothesized that catheter-based r...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2012.00134

    authors: Zuern CS,Rizas KD,Eick C,Stoleriu C,Bunk L,Barthel P,Balletshofer B,Gawaz M,Bauer A

    更新日期:2012-05-10 00:00:00

  • Unique Running Pattern and Mucosal Morphology Found in the Colon of Cotton Rats.

    abstract::Cotton rats are one of the experimental rodents used for testing different infectious and non-infectious diseases, including gastrointestinal tract pathology. However, their intestinal morphological characteristics are still poorly understood. Here, we clarified the anatomical and histological characteristics of the c...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.587214

    authors: Chuluunbaatar T,Ichii O,Nakamura T,Irie T,Namba T,Islam MR,Otani Y,Masum MA,Okamatsu-Ogura Y,Elewa YHA,Kon Y

    更新日期:2020-10-26 00:00:00

  • Cardiac Remodeling: Endothelial Cells Have More to Say Than Just NO.

    abstract::The heart is a highly structured organ consisting of different cell types, including myocytes, endothelial cells, fibroblasts, stem cells, and inflammatory cells. This pluricellularity provides the opportunity of intercellular communication within the organ, with subsequent optimization of its function. Intercellular ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2018.00382

    authors: Segers VFM,Brutsaert DL,De Keulenaer GW

    更新日期:2018-04-11 00:00:00

  • Ephrin regulation of palate development.

    abstract::Studies of palate development are motivated by the all too common incidence of cleft palate, a birth defect that imposes a tremendous health burden and can leave lasting disfigurement. Although, mechanistic studies of palate growth and fusion have focused on growth factors such as Transforming Growth Factor ß-3 (Tgfß3...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2012.00376

    authors: Benson MD,Serrano MJ

    更新日期:2012-09-26 00:00:00

  • Marine Mammals' NMDA Receptor Structure: Possible Adaptation to High Pressure Environment.

    abstract::Divers that are exposed to high pressure (HP) above 1.1 MPa suffer from High Pressure Neurological Syndrome (HPNS), which is implicated with central nervous system (CNS) malfunction. Marine mammals performing extended and deep breath-hold dives are exposed to almost 20 MPa without apparent HPNS symptoms. N-methyl-D-as...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.01633

    authors: Bliznyuk A,Golan H,Grossman Y

    更新日期:2018-11-22 00:00:00

  • More than a bystander: the contributions of intrinsic skeletal muscle defects in motor neuron diseases.

    abstract::Spinal muscular atrophy (SMA), amyotrophic lateral sclerosis (ALS), and spinal-bulbar muscular atrophy (SBMA) are devastating diseases characterized by the degeneration of motor neurons. Although the molecular causes underlying these diseases differ, recent findings have highlighted the contribution of intrinsic skele...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2013.00356

    authors: Boyer JG,Ferrier A,Kothary R

    更新日期:2013-12-18 00:00:00

  • Intracellular calcium signals display an avalanche-like behavior over multiple lengthscales.

    abstract::Many natural phenomena display "self-organized criticality" (SOC), (Bak et al., 1987). This refers to spatially extended systems for which patterns of activity characterized by different lengthscales can occur with a probability density that follows a power law with pattern size. Differently from power laws at phase t...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2012.00350

    authors: Lopez L,Piegari E,Sigaut L,Ponce Dawson S

    更新日期:2012-09-03 00:00:00

  • Post-exercise Endothelium-Dependent Vasodilation Is Dependent on Training Status.

    abstract::The effect of training status on post-exercise flow-mediated dilation (FMD) is not well characterized. We tested the hypothesis that the more trained the subjects, the lower the reduction in FMD after an acute bout of aerobic exercise. Forty-seven men (mean ± SD, age: 20.1 ± 1.2 years, body mass: 75.5 ± 5.1 kg, height...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00348

    authors: Kapilevich LV,Kologrivova VV,Zakharova AN,Mourot L

    更新日期:2020-05-08 00:00:00

  • Influence of Physical Exercise on Advanced Glycation End Products Levels in Patients Living With the Human Immunodeficiency Virus.

    abstract::Introduction: Combined antiretroviral therapy (cART) used to treat acquired immunodeficiency virus (HIV) induces a number of adverse effects, such as insulin resistance and dyslipidemia, which ultimately increases the cardiovascular risk. Advanced glycation end products (AGEs) have been implicated in the etiology of c...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.01641

    authors: Rodrigues KL,Borges JP,Lopes GO,Pereira ENGDS,Mediano MFF,Farinatti P,Tibiriça E,Daliry A

    更新日期:2018-12-06 00:00:00

  • Age-Dependent Decline in Cardiac Function in Guanidinoacetate-N-Methyltransferase Knockout Mice.

    abstract:Aim:Guanidinoacetate N-methyltransferase (GAMT) is the second essential enzyme in creatine (Cr) biosynthesis. Short-term Cr deficiency is metabolically well tolerated as GAMT-/- mice exhibit normal exercise capacity and response to ischemic heart failure. However, we hypothesized long-term consequences of Cr deficiency...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.01535

    authors: Aksentijević D,Zervou S,Eykyn TR,McAndrew DJ,Wallis J,Schneider JE,Neubauer S,Lygate CA

    更新日期:2020-01-21 00:00:00

  • Simulation of the Effects of Extracellular Calcium Changes Leads to a Novel Computational Model of Human Ventricular Action Potential With a Revised Calcium Handling.

    abstract::The importance of electrolyte concentrations for cardiac function is well established. Electrolyte variations can lead to arrhythmias onset, due to their important role in the action potential (AP) genesis and in maintaining cell homeostasis. However, most of the human AP computer models available in literature were d...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00314

    authors: Bartolucci C,Passini E,Hyttinen J,Paci M,Severi S

    更新日期:2020-04-15 00:00:00

  • High-Intensity Interval Training Is Associated With Alterations in Blood Biomarkers Related to Brain Injury.

    abstract::Purpose: Blood biomarkers are a useful tool to study concussion. However, their interpretation is complicated by a number of potential biological confounds, including exercise. This is particularly relevant in military and athletic settings where injury commonly occurs during physical exertion. The impact of high-inte...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.01367

    authors: Di Battista AP,Moes KA,Shiu MY,Hutchison MG,Churchill N,Thomas SG,Rhind SG

    更新日期:2018-09-28 00:00:00

  • Glutamate Transport and Preterm Brain Injury.

    abstract::Preterm birth complications are the leading cause of child death worldwide and a top global health priority. Among the survivors, the risk of life-long disabilities is high, including cerebral palsy and impairment of movement, cognition, and behavior. Understanding the molecular mechanisms of preterm brain injuries is...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2019.00417

    authors: Pregnolato S,Chakkarapani E,Isles AR,Luyt K

    更新日期:2019-04-24 00:00:00

  • Computerized Morphometric Analysis of Eryptosis.

    abstract::Eryptosis is the suicidal destruction-process of erythrocytes, much like apoptosis of nucleated cells, in the course of which the stressed red cell undergoes cell-shrinkage, vesiculation and externalization of membrane phosphatidylserine. Currently, there exist numerous methods to detect eryptosis, both morphometrical...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.01230

    authors: Jacob SS,Prasad K,Rao P,Kamath A,Hegde RB,Baby PM,Rao RK

    更新日期:2019-09-24 00:00:00

  • Abnormal Spontaneous Brain Activity in Early Parkinson's Disease With Mild Cognitive Impairment: A Resting-State fMRI Study.

    abstract::Mild cognitive impairment (MCI) is a common symptom at the baseline of early Parkinson's disease (PD) diagnosis, but the neural mechanism is unclear. To address the issue, the present study employed resting-state functional magnetic resonance imaging data of 19 drug-naïve PD patients with normal cognition (PD-NC), 10 ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.01093

    authors: Wang Z,Jia X,Chen H,Feng T,Wang H

    更新日期:2018-08-14 00:00:00

  • Neuregulin 4 Is a Novel Marker of Beige Adipocyte Precursor Cells in Human Adipose Tissue.

    abstract::Background: Nrg4 expression has been linked to brown adipose tissue activity and browning of white adipocytes in mice. Here, we aimed to investigate whether these observations could be translated to humans by investigating NRG4 mRNA and markers of brown/beige adipocytes in human visceral (VAT) and subcutaneous adipose...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.00039

    authors: Comas F,Martínez C,Sabater M,Ortega F,Latorre J,Díaz-Sáez F,Aragonés J,Camps M,Gumà A,Ricart W,Fernández-Real JM,Moreno-Navarrete JM

    更新日期:2019-01-31 00:00:00