Abstract:
:The two most common aging-related diseases, Alzheimer's disease and type 2 diabetes mellitus, are associated with accumulation of amyloid proteins (β-amyloid and amylin, respectively). This amylin aggregation is reportedly cytotoxic to neurons. We found that aggregation of human amylin (hAmylin) induced neuronal apoptosis without obvious microglial infiltration in vivo. High concentrations of hAmylin irreversibly aggregated on the surface of the neuronal plasma membrane. Long-term incubation with hAmylin induced morphological changes in neurons. Moreover, hAmylin permeabilized the neuronal membrane within 1 min in a manner similar to Triton X-100, allowing impermeable fluorescent antibodies to enter the neurons and stain intracellular antigens. hAmylin also permeabilized the cell membrane of astrocytes, though more slowly. Under scanning electron microscopy, we observed that hAmylin destroyed the integrity of the cell membranes of both neurons and astrocytes. Additionally, it increased intracellular reactive oxygen species generation and reduced the mitochondrial membrane potential. Thus, by aggregating on the surface of neurons, hAmylin impaired the cell membrane integrity, induced reactive oxygen species production, reduced the mitochondrial membrane potential, and ultimately induced neuronal apoptosis.
journal_name
Aging (Albany NY)journal_title
Agingauthors
Zhang N,Xing Y,Yu Y,Liu C,Jin B,Huo L,Kong D,Yang Z,Zhang X,Zheng R,Jia Z,Kang L,Zhang Wdoi
10.18632/aging.103105subject
Has Abstractpub_date
2020-05-28 00:00:00pages
8923-8938issue
10issn
1945-4589pii
103105journal_volume
12pub_type
杂志文章相关文献
Aging-US文献大全abstract::Hyperglycemia impairs insulin secretion as well as insulin action, being recognized as the glucotoxicity that accelerates diabetes. However, the mechanism underlying the glucotoxicity in pancreatic β-cells is not thoroughly understood. Hyperglycemia alters glucose metabolism within β-cells and interstitial conditions ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100647
更新日期:2014-03-01 00:00:00
abstract::Macromitophagy controls mitochondrial quality and quantity. It involves the sequestration of dysfunctional or excessive mitochondria within double-membrane autophagosomes, which then fuse with the vacuole/lysosome to deliver these mitochondria for degradation. To investigate a physiological role of macromitophagy in y...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100547
更新日期:2013-04-01 00:00:00
abstract::The tumor suppressor LKB1 has emerged as a critical regulator of cell polarity and energy‐metabolism. Studies in diverse model organisms continue to unravel the pathways downstream of LKB1; the emerging picture is that the outcomes of LKB1 signaling are mediated by a plethora of tissue‐specific and context‐dependent e...
journal_title:Aging
pub_type: 杂志文章,评审
doi:10.18632/aging.100319
更新日期:2011-04-01 00:00:00
abstract::Age can significantly affect human physiology and disease risk. Recent studies have shown that age may affect the composition and function of the gut microbiota, but the underlying mechanisms remain largely unknown. Non-human primates are an ideal model for uncovering how age shapes the gut microbiota, as their microb...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102541
更新日期:2019-12-14 00:00:00
abstract::In this study, we used murine chondrocytes as an in vitro model and mice exhibiting destabilization of the medial meniscus (DMM) as an in vivo model to investigate the mechanisms through which S-allyl cysteine (SAC) alleviates osteoarthritis (OA). SAC significantly reduced apoptosis and senescence and maintained homeo...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103757
更新日期:2020-10-07 00:00:00
abstract::The full spectrum of activities of the tumor suppressor p53 (TP53) has not been completely elucidated yet. Recently, it was demonstrated that TP53 communicates with the metabolic regulator mechanistic target of rapamycin (MTOR) to determine whether stressed cells undergo cell death, reversible quiescence or irreversib...
journal_title:Aging
pub_type: 社论
doi:10.18632/aging.100202
更新日期:2010-09-01 00:00:00
abstract::Current tumor-node-metastasis (TNM) stage is unable to accurately predict the overall survival (OS) in breast cancer (BC) patients. This study aimed to construct a microRNA (miRNA)-based model to improve survival prediction of BC. We confirmed 99 differentially expressed miRNAs (DEMs) in 1044 BC samples compared to 10...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101767
更新日期:2019-01-30 00:00:00
abstract:BACKGROUND:Chromosome 1p/19q codeletion is increasingly being recognized as the crucial genetic marker for glioma patients and have been included in WHO classification of glioma in 2016. Fluorescent in situ hybridization, a widely used method in detecting 1p/19q status, has some methodological limitations which might i...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101795
更新日期:2019-02-02 00:00:00
abstract::Long alpha-synuclein gene (SNCA) promoter (Rep1) allele-carriers are linked to higher risk for Parkinson's disease (PD) and faster motor progression. Non-motor symptoms including autonomic, neuropsychiatric, and sleep disorders are common in PD. However, the relationship between SNCA Rep1 microsatellite lengths and no...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104111
更新日期:2020-10-20 00:00:00
abstract::Type 2 Diabetes (T2D) is a chronic disease associated with a number of micro- and macrovascular complications that increase the morbidity and mortality of patients. The risk of diabetic complications has a strong genetic component. To this end, we sought to evaluate the association of 40 single nucleotide polymorphism...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101375
更新日期:2018-02-04 00:00:00
abstract::Interleukin 18 (IL-18) promotes inflammation and apoptosis in chondrocytes, thereby contributing to the development and progression of osteoarthritis (OA). Here, we investigated the effects of IL-18 treatment and inhibition in rat chondrocytes in vitro and in vivo. We used RT-PCR and Western blotting to measure the mR...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102937
更新日期:2020-03-17 00:00:00
abstract::Senescent cells (SCs) have been considered a source of age-related chronic sterile systemic inflammation and a target for anti-aging therapies. To understand mechanisms controlling the amount of SCs, we analyzed the phenomenon of rapid clearance of human senescent fibroblasts implanted into SCID mice, which can be ove...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100991
更新日期:2016-07-01 00:00:00
abstract::Our objectives were to evaluate: 1) the associations of cognitive frailty with various health outcomes including disability, hospitalization, and death; 2) whether the associations differed by multimorbidity. We included data of 5113 Chinese older adults (aged 60+ years) who had baseline cognition and physical frailty...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104078
更新日期:2020-11-18 00:00:00
abstract::Genome stability of human embryonic stem cells (hESC) is an important issue because even minor genetic alterations can negatively impact cell functionality and safety. The incorrect repair of DNA double-stranded breaks (DSBs) is the ultimate cause of the formation of chromosomal aberrations. Using G2 radiosensitivity ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100336
更新日期:2011-06-01 00:00:00
abstract::Atg7, a critical component of autophagy machinery, is essential for counteracting hematopoietic aging. However, the non-autophagic role of Atg7 on hematopoietic cells remains fundamentally unclear. In this study, we found that loss of Atg7, but not Atg5, another autophagy-essential gene, in the hematopoietic system re...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104176
更新日期:2020-11-24 00:00:00
abstract::Epigenetic regulation of various genomic functions, including gene expression, provide mechanisms whereby an organism can dynamically respond to changes in its environment and modify gene expression accordingly. One epigenetic mechanism implicated in human aging and age-related disorders is DNA methylation. Isolated p...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101187
更新日期:2017-02-28 00:00:00
abstract::Of all the major organ systems in the body, the ovaries of females are the first to exhibit impaired function with advancing age. Until recently, traditional thinking was that female mammals are provided with a non-renewable pool of oocyte-containing follicles at birth that are depleted during postnatal life to exhaus...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100105
更新日期:2009-12-12 00:00:00
abstract::Dietary supplements are widely used for health purposes. However, little is known about the metabolic and cardiovascular effects of combinations of popular over-the-counter supplements, each of which has been shown to have anti-oxidant, anti-inflammatory and pro-longevity properties in cell culture or animal studies. ...
journal_title:Aging
pub_type: 杂志文章,随机对照试验
doi:10.18632/aging.100597
更新日期:2014-02-01 00:00:00
abstract::The mechanism underlying the association between age and depletion of the human ovarian follicle reserves remains uncertain. Many identified that impaired DNA polymerase β (Pol β)-mediated DNA base-excision repair (BER) drives to mouse oocyte aging. With aging, DNA lesions accumulate in primordial follicles. However, ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.104123
更新日期:2020-11-20 00:00:00
abstract::Abnormal expression of long noncoding RNA (lncRNA) is involved in human cancers, including colorectal cancer (CRC). However, their functional mechanism is largely unknown. In this study, we explored the roles of lncRNA SOCS2-AS1 in modulating CRC progression. We showed that SOCS2-AS1 was lowly expressed in CRC tissues...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103276
更新日期:2020-05-21 00:00:00
abstract::The first few episodes of bipolar disorder (BD) are highly likely to be depressive. This phenomenon causes many BD patients to be misdiagnosed as having major depression. Therefore, it is very important to correctly diagnose BD patients during depressive episode. Here, we conducted this study to identify potential bio...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101805
更新日期:2019-02-05 00:00:00
abstract::Osteoporosis is a metabolic disease characterized by reduced osteoblast differentiation and proliferation. Oxidative stress plays a role in the pathogenesis of osteoporosis. Aucubin (AU), an iridoid glycoside, was previously shown to promote osteoblast differentiation. We investigated the effects of AU on MG63 human o...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102742
更新日期:2020-02-05 00:00:00
abstract::Recent experimental studies highlighted the role of hydrogen sulfide (H2S) in aging and longevity. The cystathionine ß-synthase (CBS) and cystathionine γ-lyase (CSE) are the key enzymes responsible for H2S production. Here we investigated the geroprotective effects of CSE and CBS overexpression in Drosophila. Overexpr...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.101630
更新日期:2018-11-08 00:00:00
abstract::N6-methyladenosine (m6A) RNA methylation, involved in cancer initiation and progression, is dynamically regulated by the m6A RNA methylation regulators. However, the expression of m6A RNA methylation regulators in ovarian cancer and their correlation with prognosis remain elusive. Here, we demonstrated that the 18 cen...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103811
更新日期:2020-09-20 00:00:00
abstract::Sarcopenia is a hallmark of aging. Inflammation due to increased generation of cytokines such as TNFα, IL-1β and IL-6 has been implicated in the pathogenesis of sarcopenia. In skeletal muscle of C57BL/6 mice from 12 until 28 months of age, we observed a progressive reduction of myofiber cross sectional area, loss of t...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202200
更新日期:2020-11-26 00:00:00
abstract::SAG is an essential RING component of the Cullin-RING ligase (CRL) E3 ubiquitin ligase complex, which regulates diverse signaling pathways and biological processes, including cell apoptosis, embryonic development, angiogenesis, and tumorigenesis. In the present study, we revealed that SAG gene expression is upregulate...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102663
更新日期:2020-01-11 00:00:00
abstract::Increasing attentions have been paid to the role of circRNAs in the etiology of triple-negative breast cancer (TNBC), and we strived to figure out the association of circRNA AKT3/miRNA axis with TNBC chemo-resistance. Altogether 207 BC patients were divided into TNBC group (n=83) and non-TNBC group (n=124), and MCF-10...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.202415
更新日期:2021-01-20 00:00:00
abstract::Non-small cell lung cancer (NSCLC) is a highly malignant tumor. Many circular RNAs (circRNAs) are reportedly in regulating the progression of NSCLC. To identify potential therapeutic targets for NSCLC, we conducted a bioinformatics analysis of circRNAs differentially expressed between NSCLC tissues and adjacent normal...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.103472
更新日期:2020-07-18 00:00:00
abstract::A major trend in recent Parkinson's disease (PD) research is the investigation of biological markers that could help in identifying at-risk individuals or to track disease progression and response to therapies. Central to this is the knowledge that inflammation is a known hallmark of PD and of many other degenerative ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.100695
更新日期:2014-10-01 00:00:00
abstract::The lack of a univocal definition of frailty, a condition frequently found in the elderly population which is correlated with an increased risk of mortality, has prompted the search for clinical and laboratory parameters associated with this condition. Whereas OPG is a protein involved in different pathophysiological ...
journal_title:Aging
pub_type: 杂志文章
doi:10.18632/aging.102083
更新日期:2019-07-17 00:00:00