Abstract:
:Rationale: Chromodomain Y-like 2 (CDYL2) is a member of the CDY gene family involved in spermatogenesis, but its role in human cancer has not been reported. Analyses of publicly available databases demonstrate that CDYL2 is abundantly expressed in breast tumors. However, whether CDYL2 is involved in breast cancer progression remains unknown. Methods: Quantitative real-time PCR and immunoblotting assays were used to determine the expression levels of CDYL2 transcript variants in breast cancer cell lines and primary breast tumors. The effect of CDYL2 transcript variants on the malignant phenotypes of breast cancer cells was examined through in vitro and in vivo assays. Immunofluorescent staining, RNA-seq, ATAC-seq, and ChIP-qPCR were used to investigate the underlying mechanisms behind the aforementioned observations. Results: Here we show that CDYL2 generated four transcript variants, named CDYL2a-CDYL2d. CDYL2a and CDYL2b were the predominant variants expressed in breast cancer cell lines and breast tumors and exerted strikingly discrete functions in breast cancer growth and metastasis. CDYL2a was upregulated in the majority of the breast cancer cell lines and tumors, and promoted breast cancer cell proliferation, colony formation in vitro, and tumorigenesis in xenografts. In contrast, CDYL2b was mainly expressed in luminal- and HER2-positive types of breast cancer cell lines and tumors, and suppressed the migratory, invasive, and metastatic potential of breast cancer cells in vitro and in vivo. Mechanistically, CDYL2a partially localized to SC35-positive nuclear speckles and promoted alternative splicing of a subset of target genes, including FIP1L1, NKTR, and ADD3 by exon skipping. Elimination of full-length FIP1L1, NKTR, and ADD3 rescued the impaired cell proliferation through CDYL2a depletion. In contrast, CDYL2b localized to heterochromatin and transcriptionally repressed several metastasis-promoting genes, including HPSE, HLA-F, and SELL. Restoration of HPSE, HLA-F, or SELL expression in CDYL2b-overexpressing cells attenuated the ability of CDYL2b to suppress breast cancer cell migration and invasion. Conclusions: Collectively, these findings establish an isoform-specific function of CDYL2 in breast cancer development and progression and highlight that pharmacological inhibition of the CDYL2a, but not the CDYL2b, isoform may be an effective strategy for breast cancer therapy.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Yang LF,Yang F,Zhang FL,Xie YF,Hu ZX,Huang SL,Shao ZM,Li DQdoi
10.7150/thno.43744subject
Has Abstractpub_date
2020-04-06 00:00:00pages
5242-5258issue
12issn
1838-7640pii
thnov10p5242journal_volume
10pub_type
杂志文章相关文献
Theranostics文献大全abstract::Rationale: Busulfan is currently an indispensable anti-cancer drug, particularly for children, but the side effects on male reproduction are so serious that critical drug management is needed to minimize any negative impact. Meanwhile, alginate oligosaccharides (AOS) are natural products with many consequent advantage...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.43189
更新日期:2020-02-10 00:00:00
abstract::The α-chemokine CXCL12 (stromal derived factor-1; SDF-1) and its corresponding G(αI) protein-coupled CXCR4 receptor axis play an important role in retention of hematopoietic stem progenitor cells (HSPCs) in bone marrow (BM) stem cell niches. CXCL12 has also been identified as a strong chemoattractant for HSPCs and imp...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.4621
更新日期:2013-01-01 00:00:00
abstract::Liquid biopsy of tumor through isolation of circulating tumor cells (CTCs) allows non-invasive, repetitive, and systemic sampling of disease. Although detecting and enumerating CTCs is of prognostic significance in metastatic cancer, it is conceivable that performing molecular and functional characterization on CTCs w...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.15359
更新日期:2016-06-15 00:00:00
abstract::Extracellular vesicles (EVs), including exosomes and microvesicles, derived from bone marrow stromal cells (BMSCs) have been demonstrated as key factors in the progression and drug resistance of multiple myeloma (MM). EV uptake involves a variety of mechanisms which largely depend on the vesicle origin and recipient c...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.47996
更新日期:2021-01-01 00:00:00
abstract::Zinc-finger proteins (ZFPs) are the largest transcription factor family in mammals, involved in the regulation of multiple physiologic processes including cell differentiation, proliferation, apoptosis and neoplastic transformation. Approximately one-third of ZFPs are Krüppel-associated box domain (KRAB)-ZFPs. Methods...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.35861
更新日期:2020-01-12 00:00:00
abstract::Cell-derived microparticles (MPs) are nano-sized vesicles released by activated cells in the extracellular milieu. They act as vectors of biological activity by carrying membrane-anchored and cytoplasmic constituents of the parental cells. Although detection and characterization of cell-derived MPs may be of high diag...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.13757
更新日期:2016-03-02 00:00:00
abstract:OBJECTIVE:Ultrasound molecular imaging (UMI) of glycoprotein (GP) IIb/IIIa receptor on activated platelets offers a unique means of identifying high-risk atherosclerosis. We hypothesized that contrast-enhanced ultrasound with microbubbles (MBs) targeted to GP IIb/IIIa could be used to detect and quantify activated plat...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.10020
更新日期:2015-02-05 00:00:00
abstract::The combination of chemotherapy and RNA interference is a promising approach for efficient cancer therapy. However, the success of such a strategy is hampered by the lack of suitable vectors to coordinate small interfering RNA (siRNA) and chemotherapeutic drug into one single platform. We herein report a novel triple-...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.13515
更新日期:2016-01-01 00:00:00
abstract::Rationale: Among the biothiols-related diseases, sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection and can result in severe oxidative stress and damage to multiple organs. In this study, we aimed to develop a fluorescence chemosensor that can both detect GSH an...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.22252
更新日期:2018-02-03 00:00:00
abstract::Acute myocardial infarction (AMI) is one of the leading causes of mortality around the world, and the inflammatory response plays a pivotal role in the progress of myocardial necrosis and ventricular remodeling, dysfunction and heart failure after AMI. Therapies aimed at modulating immune response after AMI on a molec...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.53326
更新日期:2021-01-01 00:00:00
abstract::The diseases caused by viruses posed a great challenge to human health, the development of which was driven by the imbalanced host immune response. Host innate immunity is an evolutionary old defense system that is critical for the elimination of the virus. The overactive innate immune response also leads to inflammat...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.48520
更新日期:2020-07-23 00:00:00
abstract::Rationale: Corneal transplantation is an effective treatment to corneal blindness. However, the immune rejection imperils corneal allograft survival. An interventional modality is urgently needed to inhibit immune rejection and promote allograft survival. In our previous study, subconjunctival injections of bone marro...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.31711
更新日期:2020-07-09 00:00:00
abstract::Hair regenerative medicine, a promising strategy for the treatment of hair loss, will likely involve the transplantation of autologous hair follicular stem cells (HFSCs) and dermal papilla cells (DPCs) into regions of hair loss. Cyclic hair regeneration results from the periodic partial activation of HFSCs. However, p...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.48723
更新日期:2020-09-22 00:00:00
abstract::The ability to selectively destroy cancer cells while sparing normal tissue is highly desirable during the cancer therapy. Here, magnetic targeted photothermal therapy was demonstrated by the integration of MoS2 (MS) flakes and Fe3O4 (IO) nanoparticles (NPs), where MoS2 converted near-infrared (NIR) light into heat an...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.11802
更新日期:2015-05-20 00:00:00
abstract::INTRODUCTION: According to the "glymphatic system" hypothesis, brain waste clearance is mediated by a continuous replacement of the interstitial milieu by a bulk flow of cerebrospinal fluid (CSF). Previous reports suggested that this cerebral CSF circulation is only active during general anesthesia or sleep, an effect...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19154
更新日期:2018-01-01 00:00:00
abstract::Rationale: Basal-like breast cancer (BLBC) is associated with high grade, distant metastasis, and poor prognosis; however, the mechanism underlying aggressiveness of BLBC is still unclear. Emerging evidence has suggested that phospholipid scramblase 1 (PLSCR1) is involved in tumor progression. Here, we aimed to study ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.43150
更新日期:2020-03-25 00:00:00
abstract::Intrinsic and acquired resistance to targeted therapies is a significant clinical problem in cancer. We previously showed that resistance to regorafenib, a multi-kinase inhibitor for treating colorectal cancer (CRC) patients, can be caused by mutations in the tumor suppressor FBW7, which block degradation of the pro-s...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45363
更新日期:2020-07-09 00:00:00
abstract::In biology, as every science student is made to learn, ontology recapitulates phylogeny. In medicine, however, oncology recapitulates polemology, the science of warfare: The medical establishment is transitioning from highly toxic poisons that kill rapidly dividing normal and malignant cells with little specificity to...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.5658
更新日期:2013-05-25 00:00:00
abstract::Inadequate diagnostic methods for prostate cancer lead to over- and undertreatment, and the inability to intraoperatively visualize positive margins may limit the success of surgical resection. Prostate cancer visualization could be improved by combining the complementary modalities of immuno-positron emission tomogra...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.27679
更新日期:2018-11-12 00:00:00
abstract::Identification of novel targets for triple-negative breast cancer (TNBC) is an urgent task as targeted therapies have increased the lifespans of Oestrogen Receptor +/ Progesterone Receptor + and HER2+ cancer patients. Methods: genes involved in protein processing in the endoplasmic reticulum, which have been reported ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.35383
更新日期:2019-08-21 00:00:00
abstract::Background: Lysophosphatidic acid (LPA) is a small glycerophospholipid that acts as a potent extracellular signal in various biological processes and diseases. Our previous work demonstrated that the expression of the LPA receptors LPA1 and LPA3 is elevated in the early postnatal heart. However, the role of this stage...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.47913
更新日期:2020-08-29 00:00:00
abstract::SIRT6, NAD+-dependent deacetylase sirtuin 6, has recently shown to suppress tumor growth in several types of cancer. Colon cancer is a challenging carcinoma associated with high morbidity and death. However, whether SIRT6 play a direct role in colon tumorigenesis and the underlying mechanism are not understood. Method...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.29724
更新日期:2019-04-13 00:00:00
abstract::In pancreatic cancer (PDAC) intratumor infiltration of polymorphonuclear neutrophils (PMN) is associated with histologically apparent alterations of the tumor growth pattern. The aim of this study was to examine possible associations between PMN infiltration, tumor microarchitecture, and water diffusivity in diffusion...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.21089
更新日期:2018-01-01 00:00:00
abstract::Cerebrospinal fluid (CSF) microRNAs (miRNAs) have emerged as potential biomarkers for minimally invasive diagnosis of central nervous system malignancies. However, despite significant advances in recent years, this field still suffers from poor data reproducibility. This is especially true in cases of infants, conside...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.31502
更新日期:2019-06-19 00:00:00
abstract::The development of nanomedicine is expected to provide an innovative direction for addressing challenges associated with multidrug-resistant (MDR) bacteria. In the past decades, although nanotechnology-based phototherapy has been developed for antimicrobial treatment since it rarely causes bacterial resistance, the cl...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.52729
更新日期:2020-10-30 00:00:00
abstract::Long non-coding RNAs (lncRNAs) have emerged as integral components of E2F1-regulated gene regulatory networks (GRNs), but their implication in advanced or treatment-refractory malignancy is unknown. Methods: We combined high-throughput transcriptomic approaches with bioinformatics and structure modeling to search for ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44176
更新日期:2020-07-29 00:00:00
abstract::Image guided technique is playing an increasingly important role in the investigation of the biodistribution and pharmacokinetics of drugs or drug delivery systems. The application of these new materials and techniques with combined properties of diagnosis and therapy can benefit the development of targeted drug deliv...
journal_title:Theranostics
pub_type: 社论
doi:10.7150/thno.5321
更新日期:2012-01-01 00:00:00
abstract::Aims: Pathological cardiac fibrosis and hypertrophy are common features of left ventricular remodeling that often progress to heart failure (HF). Endothelial cells (ECs) are the most abundant non-myocyte cells in adult mouse heart. Simvastatin, a strong inducer of Krüppel-like Factor 2 (Klf2) in ECs, ameliorates press...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.48153
更新日期:2021-01-01 00:00:00
abstract::Stem cell tracking is a highly important subject. Current techniques based on nanoparticle-labeling, such as magnetic resonance imaging, fluorescence microscopy, and micro-computed tomography, are plagued by limitations including relatively low sensitivity or penetration depth, involvement of ionizing irradiation, and...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.5369
更新日期:2013-07-19 00:00:00
abstract::One of the major challenges in the hepatocellular carcinoma (HCC) treatment is its insensitivity to chemotherapeutic drugs. Here, we report the development of novel lipid-coated cisplatin nanoparticles co-loaded with microRNA-375 (NPC/miR-375) as a potential treatment for chemotherapy insensitive HCC. The NPC/miR-375 ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.13130
更新日期:2016-01-01 00:00:00