Abstract：
:BACKGROUND The current research work aimed to explore the protective role of myricetin against cataractogenesis in humans, in terms of its anti-apoptotic potential. MATERIAL AND METHODS Human eye lens epithelial cells were exposed to oxidative stress by treating with hydrogen peroxide (H₂O₂). The levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) were determined using standard detection kits. DAPI (4',6-diamidino-2-phenylindole), AO/EB (acridine orange/ethidium bromide) and Annexin V/propidium iodide (PI) staining assays were used for the assessment of cell apoptosis. Western blotting was used to examine the protein concentrations. RESULTS The exposure of human epithelial eye lens cells to H₂O₂ led to significant accumulation of reactive oxygen species molecules. Treatment of the H₂O₂-stressed epithelial cells with myricetin caused significant (P<0.05) increased levels of SOD, CAT, and GSH. Western blot analysis also showed a significant (P<0.05) increase in the expression of SOD, CAT, and GSH levels in human epithelial eye lens cells. Additionally, myricetin administration to H₂O₂-treated epithelial eye lens cells caused a significant decline in cell apoptosis ratio. The induction of apoptosis was associated with upregulation of Bax and downregulation of Bcl-2. CONCLUSIONS The results of this study showed the potential of myricetin in protecting the apoptosis driven cataract formation in humans.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Yang Y,Xu X,Liu Q,Huang H,Huang X,Lv Hdoi
10.12659/MSM.922519subject
Has Abstractpub_date
2020-04-26 00:00:00pages
e922519eissn
1234-1010issn
1643-3750pii
922519journal_volume
26pub_type
杂志文章abstract：:BACKGROUND To explore the effect of hypoxic preconditioning on the JAK/STAT3 signaling pathway and its effect on trophoblast cell viability and angiogenesis in preeclampsia (PE). MATERIAL AND METHODS Placental tissues from normal pregnant women and PE patients were collected to detect the expression levels of JAK and ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.905418
更新日期：2017-10-14 00:00:00
abstract：:BACKGROUND The objective of this paper was to assess the complications following sentinel lymph node biopsy (SLNB) in breast cancer patients using the SentiMag® method. MATERIAL AND METHODS The study material consisted of 368 patients who had received the SLNB procedure in combination with wide local excision (WLE), s...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.912758
更新日期：2019-04-29 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.904271
更新日期：2017-04-29 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.895689
更新日期：2016-06-20 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,随机对照试验
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更新日期：2018-12-04 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.906781
更新日期：2017-10-17 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.897542
更新日期：2017-08-17 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.892515
更新日期：2015-03-03 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
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更新日期：2017-02-18 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,随机对照试验
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.914222
更新日期：2018-12-17 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.889605
更新日期：2013-10-21 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.910184
更新日期：2018-05-16 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.915845
更新日期：2019-09-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.904220
更新日期：2017-09-14 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.902137
更新日期：2017-08-16 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.909146
更新日期：2018-10-30 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.894238
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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doi：
更新日期：2002-03-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.924202
更新日期：2020-08-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.904318
更新日期：2017-10-19 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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doi：10.12659/msm.904374
更新日期：2017-04-23 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.892990
更新日期：2015-05-13 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.909736
更新日期：2018-11-08 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.909188
更新日期：2018-08-17 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2001-09-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
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更新日期：2018-09-25 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
