Abstract:
:Meniscus deficiency, the most common and refractory disease in human knee joints, often progresses to osteoarthritis (OA) due to abnormal biomechanical distribution and articular cartilage abrasion. However, due to its anisotropic spatial architecture, complex biomechanical microenvironment, and limited vascularity, meniscus repair remains a challenge for clinicians and researchers worldwide. In this study, we developed a 3D printing-based biomimetic and composite tissue-engineered meniscus scaffold consisting of polycaprolactone (PCL)/silk fibroin (SF) with extraordinary biomechanical properties and biocompatibility. We hypothesized that the meticulously tailored composite scaffold could enhance meniscus regeneration and cartilage protection. Methods: The physical property of the scaffold was characterized by scanning electron microscopy (SEM) observation, degradation test, frictional force of interface assessment, biomechanical testing, and fourier transform infrared (FTIR) spectroscopy analysis. To verify the biocompatibility of the scaffold, the viability, morphology, proliferation, differentiation, and extracellular matrix (ECM) production of synovium-derived mesenchymal stem cell (SMSC) on the scaffolds were assessed by LIVE/DEAD staining, alamarBlue assay, ELISA analysis, and qRT-PCR. The recruitment ability of SMSC was tested by dual labeling with CD29 and CD90 by confocal microscope at 1 week after implantation. The functionalized hybrid scaffold was then implanted into the meniscus defects on rabbit knee joint for meniscus regeneration, comparing with the Blank group (no scaffold) and PS group. The regenerated meniscus tissue was evaluated by histological and immunohistochemistry staining, and biomechanical test. Macroscopic and histological scoring was performed to assess the outcome of meniscus regeneration and cartilage protection in vivo. Results: The combination of SF and PCL could greatly balance the biomechanical properties and degradation rate to match the native meniscus. SF sponge, characterized by fine elasticity and low interfacial shear force, enhanced energy absorption capacity of the meniscus and improved chondroprotection. The SMSC-specific affinity peptide (LTHPRWP; L7) was conjugated to the scaffold to further increase the recruitment and retention of endogenous SMSCs. This meticulously tailored scaffold displayed superior biomechanics, structure, and function, creating a favorable microenvironment for SMSC proliferation, differentiation, and extracellular matrix (ECM) production. After 24 weeks of implantation, the histological assessment, biochemical contents, and biomechanical properties demonstrated that the polycaprolactone/silk fibroin-L7 (PS-L7) group was close to the native meniscus group, showing significantly better cartilage protection than the PS group. Conclusion: This tissue engineering scaffold could greatly strengthen meniscus regeneration and chondroprotection. Compared with traditional cell-based therapies, the meniscus tissue engineering approach with advantages of one-step operation and reduced cost has a promising potential for future clinical and translational studies.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Li Z,Wu N,Cheng J,Sun M,Yang P,Zhao F,Zhang J,Duan X,Fu X,Zhang J,Hu X,Chen H,Ao Ydoi
10.7150/thno.44270subject
Has Abstractpub_date
2020-04-06 00:00:00pages
5090-5106issue
11issn
1838-7640pii
thnov10p5090journal_volume
10pub_type
杂志文章相关文献
Theranostics文献大全abstract::The diseases caused by viruses posed a great challenge to human health, the development of which was driven by the imbalanced host immune response. Host innate immunity is an evolutionary old defense system that is critical for the elimination of the virus. The overactive innate immune response also leads to inflammat...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.48520
更新日期:2020-07-23 00:00:00
abstract::Rationale: Saliva as a sample matrix is rapidly gaining interest for disease diagnosis and point-of-care assays because it is easy to collect (non-invasive) and contains many health-related biomarkers. However, saliva poses particular problems relative to more common urine and blood matrices, which includes low analyt...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.24941
更新日期:2018-06-13 00:00:00
abstract::Rationale: Tumor angiogenesis promotes tumor development, progression, growth, and metastasis. Metronomic chemotherapy involves the frequent administration of low-dose chemotherapeutic agents to block angiogenic activity and reduce side effects. Methods: MDA-MB-231 cells were treated with various concentrations of art...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.33353
更新日期:2019-09-17 00:00:00
abstract::Heart diseases are one of the leading causes of death for humans in the world. Increasing evidence has shown that myocardial injury induced innate and adaptive immune responses upon early cellular damage but also during chronic phases post-injury. The immune cells can not only aggravate the injury but also play an ess...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.33112
更新日期:2019-05-31 00:00:00
abstract::Background: Programmed cell death protein 1 (PD1) inhibitors have revolutionized cancer therapy, yet many patients fail to respond. Thus, the identification of accurate predictive biomarkers of therapy response will improve the clinical benefit of anti-PD1 therapy. Method: We assessed the baseline serological autoanti...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45816
更新日期:2020-05-16 00:00:00
abstract::Cancer theranostics is one of the most important approaches for detecting and treating patients at an early stage. To develop such a technique, accurate detection, specific targeting, and controlled delivery are the key components. Various kinds of nanoparticles have been proposed and demonstrated as potential nanoveh...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.27828
更新日期:2018-11-29 00:00:00
abstract::Protein therapeutics is playing an increasingly critical role in treatment of human diseases. However, current vectors are captured by the digestive endo-lysosomal system, which results in an extremely low fraction (<2%) of protein being released in the cytoplasm. This paper reports a drug-delivering-drug platform (HA...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.23804
更新日期:2018-06-06 00:00:00
abstract::Rationale: Loss of DNA damage repair (DDR) in the tumor is an established hallmark of sensitivity to DNA damaging agents such as chemotherapy. However, there has been scant investigation into gain-of-function alterations of DDR genes in cancer. This study aims to investigate to what extent copy number amplification of...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.39341
更新日期:2020-03-04 00:00:00
abstract::Successful anticancer chemotherapy requires targeting tumors efficiently and further potential to eliminate cancer stem cell (CSC) subpopulations. Since CD44 is present on many types of CSCs, and it binds specially to hyaluronic acid (HA), we tested whether coating solid lipid nanoparticles with hyaluronan (HA-SLNs)wo...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.10804
更新日期:2015-04-05 00:00:00
abstract::The delivery of therapeutics into tumors remains a challenge in nanoparticle-mediated drug delivery. However, effective therapies such as photothermal therapy (PTT) are limited by quick systemic clearance and non-specific biodistribution. Anti-tumor strategies tailored to accommodate both tumor accumulation/retention ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.33280
更新日期:2019-04-13 00:00:00
abstract::Body fluids are a rich source of extracellular vesicles (EVs), which carry cargo derived from the secreting cells. So far, biomarkers for pathological conditions have been mainly searched from their protein, (mi)RNA, DNA and lipid cargo. Here, we explored the small molecule metabolites from urinary and platelet EVs re...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19890
更新日期:2017-08-23 00:00:00
abstract::Mitochondria-mediated oxidative stress and apoptosis contribute greatly to early brain injury (EBI) following subarachnoid hemorrhage (SAH). This study hypothesized that activation of melanocortin 1 receptor (MC1R), using BMS-470539, attenuates EBI by controlling mitochondrial metabolism after SAH. Methods: We utilize...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.49426
更新日期:2021-01-01 00:00:00
abstract::Because of the unparalleled efficiency and universal utility in treating a variety of disease types, siRNA agents have evolved as the future drug-of-choice. Yet, the inability of the polyanionic siRNA macromolecules to cross the cell membrane remains as the bottleneck of possible clinical applications. With the cell p...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19863
更新日期:2017-06-25 00:00:00
abstract::Hair regenerative medicine, a promising strategy for the treatment of hair loss, will likely involve the transplantation of autologous hair follicular stem cells (HFSCs) and dermal papilla cells (DPCs) into regions of hair loss. Cyclic hair regeneration results from the periodic partial activation of HFSCs. However, p...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.48723
更新日期:2020-09-22 00:00:00
abstract::Long term pulmonary exposure to crystalline silica leads to silicosis that manifests progressive interstitial fibrosis, eventually leading to respiratory failure and death. Despite efforts to eliminate silicosis, clinical cases continue to occur in both developing and developed countries. The exact mechanisms of cryst...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.16180
更新日期:2016-09-09 00:00:00
abstract::Overexpression of CD146 has been correlated with aggressiveness, recurrence rate, and poor overall survival in hepatocellular carcinoma (HCC) patients. In this study, we set out to develop a CD146-targeting probe for high-contrast noninvasive in vivo positron emission tomography (PET) and near-infrared fluorescence (N...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.15568
更新日期:2016-08-08 00:00:00
abstract::Rationale: Mxi1 is regarded as a potential tumor suppressor protein that antagonizes the transcriptional activity of proto-oncogene Myc. However, the clinical significances and underlying mechanisms by which Mxi1 is regulated in lung cancer remain poorly understood. Methods: Mass spectrometry analysis and immunoprecip...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.22552
更新日期:2018-02-02 00:00:00
abstract::In ultrasound molecular imaging (USMI), ligand-functionalized microbubbles (MBs) are used to visualize vascular endothelial targets. Netrin-1 is upregulated in 60% of metastatic breast cancers and promotes tumor progression. A novel netrin-1 interference therapy requires the assessment of netrin-1 expression prior to ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.27221
更新日期:2018-10-06 00:00:00
abstract::Cell-based immunotherapy for the treatment of hematologic malignancies, such as leukemia and lymphoma, has seen much success and played an increasingly important role in clinical studies. Nevertheless, the efficacy of immunotherapy in solid tumors still needs improvements due to the immunosuppressive properties of tum...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.35900
更新日期:2019-10-14 00:00:00
abstract::Long non-coding RNAs (lncRNAs) have emerged as integral components of E2F1-regulated gene regulatory networks (GRNs), but their implication in advanced or treatment-refractory malignancy is unknown. Methods: We combined high-throughput transcriptomic approaches with bioinformatics and structure modeling to search for ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44176
更新日期:2020-07-29 00:00:00
abstract::Proper selection and effective delivery of combination drugs targeting multiple pathophysiological pathways key to spinal cord injury (SCI) hold promise to address the thus far scarce clinical therapeutics for improving recovery after SCI. In this study, we aim to develop a clinically feasible way for targeted deliver...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.26717
更新日期:2018-08-07 00:00:00
abstract::Producing keratinocyte cells (KCs) in large scale is difficult due to their slow proliferation, disabling their use as seed cells for skin regeneration and wound healing. Cell reprogramming is a promising inducer-based approach to KC production but only reaches very low cellular conversion. Here we reported a unique c...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.31526
更新日期:2019-07-09 00:00:00
abstract::Luminal androgen receptor (LAR) breast cancer accounts for 10% of all triple-negative breast cancers (TNBC). Anti-androgen therapy for this subtype is in development, but yields only partial clinical benefits. In this study, we aimed to characterize the genomic alterations of LAR TNBC, to analyze activation of the PI3...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.36182
更新日期:2020-01-01 00:00:00
abstract::Arachidonic acid (AA) is a polyunsaturated fatty acid present at high concentrations in the ovarian cancer (OC) microenvironment and associated with a poor clinical outcome. In the present study, we have unraveled a potential link between AA and macrophage functions. Methods: AA-triggered signal transduction was studi...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.52442
更新日期:2021-01-01 00:00:00
abstract::Background: Metastasis is the major reason for high recurrence rates and poor survival among patients with colorectal cancer (CRC). However, the underlying molecular mechanism of CRC metastasis is unclear. This study aimed to investigate the role of forkhead box K2 (FOXK2), one of the most markedly increased FOX genes...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.31716
更新日期:2019-05-31 00:00:00
abstract::Müller glia are specialized retinal cells with stem cell properties in fish and frogs but not in mammals. Current efforts to develop gene therapies to activate mammalian Müller glia for retinal repair will require safe and effective delivery strategies for recombinant adeno-associated viruses (AAVs), vectors of choice...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.42611
更新日期:2020-02-10 00:00:00
abstract::Model observers play an important role in the optimization and assessment of imaging devices. In this review paper, we first discuss the basic concepts of model observers, which include the mathematical foundations and psychophysical considerations in designing both optimal observers for optimizing imaging systems and...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.5138
更新日期:2013-10-04 00:00:00
abstract::Systemic infusion of bone marrow-derived mesenchymal stem cells (BMSCs) has become a promising strategy for disease treatment and tissue regeneration. Strategies to enhance the efficiency of BMSC cell therapy are crucial to promote its clinical application. Here, we aimed to improve BMSC cell therapy by inhibiting the...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.16911
更新日期:2017-01-01 00:00:00
abstract::Mitochondria in cancer cells maintain a more negative membrane potential than normal cells. Mitochondria are the primary source of cellular reactive oxygen species (ROS), which are necessary for photodynamic therapy. Thus, the strategy of targeting mitochondria can maximize the photodynamic therapeutic efficiency for ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.15433
更新日期:2016-10-01 00:00:00
abstract:OBJECTIVE:Ultrasound molecular imaging (UMI) of glycoprotein (GP) IIb/IIIa receptor on activated platelets offers a unique means of identifying high-risk atherosclerosis. We hypothesized that contrast-enhanced ultrasound with microbubbles (MBs) targeted to GP IIb/IIIa could be used to detect and quantify activated plat...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.10020
更新日期:2015-02-05 00:00:00