Can Keratin Scaffolds be used for Creating Three-dimensional Cell Cultures?

Abstract:

:Three-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

journal_name

Open Med (Wars)

authors

Bochynska-Czyz M,Redkiewicz P,Kozlowska H,Matalinska J,Konop M,Kosson P

doi

10.1515/med-2020-0031

subject

Has Abstract

pub_date

2020-04-03 00:00:00

pages

249-253

issn

2391-5463

pii

med-2020-0031

journal_volume

15

pub_type

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