Abstract:
:Accumulating evidence indicates that competing endogenous RNA networks play a critical role in cirrhosis progression. However, their biological role and regulatory mechanisms in liver sinusoidal endothelial cells (LSECs) have not been explored. Here, we exposed LSECs to starvation and lipopolysaccharide (LPS) treatment and assessed changes in TUG1 and miR-142-3p expression, autophagy, and endothelial-mesenchymal transition (EndMT). We confirmed the effects of targeted binding between miR-142-3p and TUG1 or ATG5 by luciferase activity and radio-immunoprecipitation assay. Using an in vivo rat model of cirrhosis, we evaluated autophagy and EndMT in LSECs by immunofluorescence co-localization and immunohistochemical staining. The diagnostic efficiency of miR-142-3p and LPS were determined by receiver-operating characteristic curve analysis. We found that LSECs survived starvation by activating autophagy. LPS treatment enhanced autophagy and promoted EndMT of LSECs by upregulating TUG1. Our rat model of cirrhosis confirmed that serum LPS level, autophagy, and EndMT were increased in LSECs. TUG1 was highly expressed in LSECs, and TUG1 knockdown suppressed ATG5-mediated autophagy and EndMT of LSECs. TUG1 regulated ATG5 via shared miR-142-3p response elements. miR-142-3p was expressed at low levels in LSECs and negatively regulated autophagy and EndMT by reducing ATG5 expression. Our results suggest that TUG1 promotes LPS-induced autophagy and EndMT of LSECs by functioning as an endogenous sponge for miR-142-3p and promoting the expression of ATG5. LPS and miR-142-3p are potential diagnostic and therapeutic targets in cirrhosis.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Zhang R,Huang XQ,Jiang YY,Li N,Wang J,Chen SYsubject
Has Abstractpub_date
2020-03-15 00:00:00pages
758-772issue
3issn
1943-8141journal_volume
12pub_type
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-12-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-04-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-09-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2016-02-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2018-02-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2019-09-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2017-08-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
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更新日期:2020-12-15 00:00:00
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journal_title:American journal of translational research
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journal_title:American journal of translational research
pub_type:
doi:
更新日期:2017-04-15 00:00:00
abstract:BACKGROUND:Infection can induce and aggravate chronic kidney disease (CKD), and the chemotaxis of Th22 cells may aggravate CKD. However, the mechanism underlying group A Streptococcus (GAS) infections in CKD through the chemotaxis of Th22 cells remains unknown. METHODS:The experiment was divided into a normal control ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-12-15 00:00:00
abstract::Severe acute pancreatitis (SAP) contributes to multiple organ dysfunction and intestine is one of the most susceptible targets. This study aims to explore the role of C3a/C3aR axis in SAP-induced intestinal barrier injury. Adult male Sprague Dawley rats were randomly divided into control, SAP, C3aRA (0.06 mg/kg) and C...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-10-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2019-04-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2019-09-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-03-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-06-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-06-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-07-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-10-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-07-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-08-15 00:00:00
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journal_title:American journal of translational research
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更新日期:2017-02-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-02-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-10-15 00:00:00
abstract::Focal liver lesions (FLLs) include a variety of benign and malignant tumors, and are usually differential diagnosed by ultrasonography. However, traditional two-dimensional contrast-enhanced ultrasound (2D-CEUS) can evaluate a single slice of a lesion. It cannot fully display perfusion of a lesion and the relationship...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-05-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-09-15 00:00:00
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journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-01-15 00:00:00
abstract::[This corrects the article on p. 1295 in vol. 10, PMID: 29887946.]. ...
journal_title:American journal of translational research
pub_type: 杂志文章,已发布勘误
doi:
更新日期:2018-07-15 00:00:00
abstract:PURPOSE:This study aimed to determine whether chronic intraocular pressure (IOP) elevation induces retinal oxidative stress and alters mitochondrial morphology and function of retinal ganglion cells (RGC) and to explore the effects of AAV-SOD2 gene therapy on the RGC survival and mitochondrial dysfunction. METHODS:Chr...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-02-15 00:00:00