Abstract:
:Rationale: Mesenchymal cell-derived osteosarcoma is a rare malignant bone tumor affecting children and adolescents. PTEN down-regulation or function-loss mutation is associated with the aggressive of osteosarcoma. Explicating the regulatory mechanism of PTEN might highlight new targets for improving the survival rate of osteosarcoma patients. Methods: The clinical relevance of FGD1 was examined by the TCGA data set, Western blotting and immunohistochemistry of osteosarcoma microarray slides. Functional assays, such as the MTS assay, colony formation assay and xenografts, were used to determine the biological role of FGD1 in osteosarcoma. The protein-protein interaction between FGD1 and PTEN was detected via co-immunoprecipitation. The relationship between FGD1 and PD-L1 was examined by Western blot analysis, RT-qPCR and immunohistochemistry. Results: In this study, analysis of the TCGA data set of sarcomas revealed that FGD1 was over-expressed with the highest P values. Then, we demonstrated that FGD1 was also abnormally up-regulated in osteosarcoma with unfavorable prognosis. Aberrant expressed FGD1 promoted the osteosarcoma tumor cell proliferation and invasion. Moreover, we found that FGD1 was participated in activating PI3K/AKT signaling pathway by interacting with PTEN. Finally, we showed that FGD1 was capable of regulating the tumor immune response via the PTEN/PD-L1 axis in osteosarcoma. Conclusions: Our data suggested that abnormally over-expressed FGD1 functions as an oncogenic protein to promote osteosarcoma progression through inhibiting PTEN activity and activating PI3K/AKT signaling. Notably, FGD1 increased PD-L1 expression in a PTEN dependent manner and modulated the sensitivity of immune checkpoint-based immunotherapy in osteosarcoma. Thus, FGD1 might be a potential target for improving the survival rate of osteosarcomas.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Wu W,Jing D,Meng Z,Hu B,Zhong B,Deng X,Jin X,Shao Zdoi
10.7150/thno.41279subject
Has Abstractpub_date
2020-02-03 00:00:00pages
2859-2871issue
6issn
1838-7640pii
thnov10p2859journal_volume
10pub_type
杂志文章相关文献
Theranostics文献大全abstract::Rationale: P21-activated kinase 6 (PAK6) is a member of the class II PAKs family, which is a conserved family of serine/threonine kinases. Although the effects of PAK6 on many malignancies, especially in prostate cancer, have been studied for a long time, the role of PAK6 in mitochondria remains unknown. Methods: The ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.42874
更新日期:2020-02-03 00:00:00
abstract::Iron oxide nanoparticles are a useful diagnostic contrast agent and have great potential for therapeutic applications. Multiple emerging diagnostic and therapeutic applications and the numerous versatile parameters of the nanoparticle platform require a robust biological model for characterization and assessment. Here...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3568
更新日期:2012-01-01 00:00:00
abstract::Radiotherapy is an extensively used treatment modality in the clinic and can kill malignant cells by generating cytotoxic reactive oxygen species (ROS). Unfortunately, excessive dosages of radiation are typically required because only a small proportion of the radiative energy is adsorbed by the soft tissues of a tumo...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.28033
更新日期:2019-01-01 00:00:00
abstract::In this study, we investigated the induction of apoptosis by ultrasound in the presence of the novel porphyrin derivative DCPH-P-Na(I). HL-60 cells were exposed to ultrasound for up to 3 min in the presence and absence of DCPH-P-Na(I), and the induction of apoptosis was examined by analyzing cell morphology, DNA fragm...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3899
更新日期:2012-01-01 00:00:00
abstract::Molecular subtyping of breast cancer is of considerable interest owing to its potential for personalized therapy and prognosis. However, current methodologies cannot be used for precise subtyping, thereby posing a challenge in clinical practice. The aim of the present study is to develop a cell-specific single-strande...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.28949
更新日期:2018-11-10 00:00:00
abstract::Aim: Immune responsive 12/15 lipoxygenase (12/15LOX)-orchestrate biosynthesis of essential inflammation-resolution mediators during acute inflammatory response in post-myocardial infarction (MI). Lack of 12/15LOX dampens proinflammatory mediator 12-(S)-hydroxyeicosatetraenoic acid (12-(S)-HETE), improves post-MI survi...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.51183
更新日期:2021-01-01 00:00:00
abstract::Biological cancer therapies, such as oncolytic, or replication-selective viruses have advantages over traditional therapeutics as they can employ multiple different mechanisms to target and destroy cancers (including direct cell lysis, immune activation and vascular collapse). This has led to their rapid recent clinic...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3724
更新日期:2012-01-01 00:00:00
abstract::Cancer vaccines have encountered their ideal personalized partner along with evidence for great breakthroughs in the identification and synthesis of neoantigens. Individual cancer neoantigen vaccines are capable of eliciting robust T-cell responses and have been demonstrated to achieve striking clinical efficacy due t...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.24387
更新日期:2018-07-30 00:00:00
abstract::RNA molecules (e.g., siRNA, microRNA, and mRNA) have shown tremendous potential for immunomodulation and cancer immunotherapy. They can activate both innate and adaptive immune system responses by silencing or upregulating immune-relevant genes. In addition, mRNA-based vaccines have recently been actively pursued and ...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.35568
更新日期:2020-01-01 00:00:00
abstract::To enhance effective drug accumulation in drug-resistant tumors, a site-specific drug-releasing polypeptide system (PEG-Phis/Pasp-DOX/CA4) was exploited in response to tumor extracellular and intracellular pH. This system could firstly release the embedded tumor vascular inhibitor (CA4) to transiently 'normalize' vasc...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.11821
更新日期:2015-04-28 00:00:00
abstract::The limited clinical efficacy of monotherapies in the clinic has urged the development of novel combination platforms. Taking advantage of light-triggered photodynamic treatment combined together with the controlled release of nanomedicine, it has been possible to treat cancer without eliciting any adverse effects. Ho...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.27721
更新日期:2018-11-03 00:00:00
abstract::Metastasis is the primary cause of death in patients with advanced cancer. Recently, a high-fat diet was shown to specifically promote the metastatic potential of specific cancer cells in a CD36-dependent manner. However, the molecular basis of the fatty acid (FA)-induced upregulation of CD36 has remained unclear. Met...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.34024
更新日期:2019-07-09 00:00:00
abstract::Stem cell therapy holds promise for treatment of intractable diseases and injured organs. For clinical translation, it is pivotal to understand the homing, engraftment, and differentiation processes of stem cells in a living body. Here we report near-infrared (NIR) fluorescent semiconductor polymer dots (Pdots) for br...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.18614
更新日期:2017-04-10 00:00:00
abstract::Zinc-finger proteins (ZFPs) are the largest transcription factor family in mammals, involved in the regulation of multiple physiologic processes including cell differentiation, proliferation, apoptosis and neoplastic transformation. Approximately one-third of ZFPs are Krüppel-associated box domain (KRAB)-ZFPs. Methods...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.35861
更新日期:2020-01-12 00:00:00
abstract::Rationale: Glioma stem-like cells (GSCs) contribute to temozolomide (TMZ) resistance in gliomas, although the mechanisms have not been delineated. Methods:In vitro functional experiments (colony formation assay, flow cytometric analysis, TUNEL assay) were used to assess the ability of extracellular vesicles (EVs) from...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.47057
更新日期:2021-01-01 00:00:00
abstract::Great progress has been made in the field of tumor immunotherapy in the past decade. However, the therapeutic effects of immune checkpoint blockade (ICB) against ovarian cancer are still limited. Recently, an inhibitor of cyclin-dependent kinases 4 and 6 (CDK4/6i) has been reported to enhance antitumor immunity in pre...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44871
更新日期:2020-08-25 00:00:00
abstract::Fast clearance, metabolism and systemic toxicity are major limits for the clinical use of anti-cancer drugs. Histone deacetylase inhibitors (HDACi) present these defects despite displaying promising anti-tumor properties on tumor cells in vitro and in in vivo model of cancers. Specific delivery of anti-cancer drugs in...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.13725
更新日期:2016-03-25 00:00:00
abstract::[This corrects the article DOI: 10.7150/thno.21516.]. ...
journal_title:Theranostics
pub_type: 已发布勘误
doi:10.7150/thno.42847
更新日期:2020-01-18 00:00:00
abstract::Müller glia are specialized retinal cells with stem cell properties in fish and frogs but not in mammals. Current efforts to develop gene therapies to activate mammalian Müller glia for retinal repair will require safe and effective delivery strategies for recombinant adeno-associated viruses (AAVs), vectors of choice...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.42611
更新日期:2020-02-10 00:00:00
abstract::Pulsed focused ultrasound (pFUS) technology is being developed for clinical neuro/immune modulation and regenerative medicine. Biological signal transduction of pFUS forces can require mechanosensitive or voltage-gated plasma membrane ion channels. Previous studies suggested pFUS is capable of activating either channe...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.33876
更新日期:2019-07-28 00:00:00
abstract::Detection of biomarkers is extremely important for the early diagnosis of diseases. Here, we developed an easy and highly sensitive fluorescence detection system for the determination of biomarkers by combining the rapid separation of magnetic beads and silver nanoparticles labeled antibodies. An ultrasensitive silver...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.17575
更新日期:2017-02-08 00:00:00
abstract::Proper selection and effective delivery of combination drugs targeting multiple pathophysiological pathways key to spinal cord injury (SCI) hold promise to address the thus far scarce clinical therapeutics for improving recovery after SCI. In this study, we aim to develop a clinically feasible way for targeted deliver...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.26717
更新日期:2018-08-07 00:00:00
abstract::In ultrasound molecular imaging (USMI), ligand-functionalized microbubbles (MBs) are used to visualize vascular endothelial targets. Netrin-1 is upregulated in 60% of metastatic breast cancers and promotes tumor progression. A novel netrin-1 interference therapy requires the assessment of netrin-1 expression prior to ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.27221
更新日期:2018-10-06 00:00:00
abstract::Body fluids are a rich source of extracellular vesicles (EVs), which carry cargo derived from the secreting cells. So far, biomarkers for pathological conditions have been mainly searched from their protein, (mi)RNA, DNA and lipid cargo. Here, we explored the small molecule metabolites from urinary and platelet EVs re...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.19890
更新日期:2017-08-23 00:00:00
abstract::The diseases caused by viruses posed a great challenge to human health, the development of which was driven by the imbalanced host immune response. Host innate immunity is an evolutionary old defense system that is critical for the elimination of the virus. The overactive innate immune response also leads to inflammat...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.48520
更新日期:2020-07-23 00:00:00
abstract::Constructing nanosystems that synergistically combine therapeutic and diagnostic features is of great interest to the nanomedicine community but also remains a tremendous challenge. Methods: In this work, we report novel catalytic nanoparticles composed of the enzyme catalase, encapsulated in a polymer shell and surfa...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.37054
更新日期:2019-10-17 00:00:00
abstract::Folate receptor (FR) has proven to be a valuable target for chemotherapy using folic acid (FA) conjugates. However, FA-conjugated chemotherapeutics still have low therapeutic efficacy accompanied with side effects, resulting from complications such as short circulation half-life, limited tumor delivery, as well as hig...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.24382
更新日期:2018-02-16 00:00:00
abstract::Mesenchymal stem cells (MSC) derived from adult tissues effectively promote wound healing. However, MSC quality varies, and the quantity of MSC is limited, as MSC are acquired through donations. Moreover, the survival and functioning of dissociated MSC delivered to an inflammatory lesion are subject to challenges. Met...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.32982
更新日期:2019-08-14 00:00:00
abstract::[This corrects the article DOI: 10.7150/thno.27882.]. ...
journal_title:Theranostics
pub_type: 杂志文章,已发布勘误
doi:10.7150/thno.49958
更新日期:2020-07-10 00:00:00
abstract::Hypoxic zones are common features of metastatic tumors. Due to inactivation of the von Hippel-Lindau gene (VHL), renal cell carcinomas (RCC) show constitutive stabilization of the alpha subunit of the hypoxia-inducible factor (HIF). Thus, RCC represents a model of chronic hypoxia. Development of the lymphatic network ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.27794
更新日期:2019-01-21 00:00:00