Single-cell RNA sequencing of immune cells in gastric cancer patients.


:Cancer immunotherapy has achieved positive clinical responses in the treatment of various cancers, including gastric cancer (GC). In this study, we characterized the heterogeneity of T cells isolated from GC patients at the single-cell level using single-cell RNA sequencing. We identified different immune cell subtypes and their heterogeneous transcription factors and depicted their developmental trajectories. In particular, we focused on exhausted CD8+ cells and Tregs and discovered that, as compared to control, the IRF8 transcription factor was downregulated in CD8+ tumour-infiltrating lymphocytes (TILs) from GC tissues, and that GC patients with lower IRF8 levels in blood CD8+ T cells tended to be a at a more advanced disease stage. These findings provide a theoretical basis for targeted immune therapy in GC.


Aging (Albany NY)




Fu K,Hui B,Wang Q,Lu C,Shi W,Zhang Z,Rong D,Zhang B,Tian Z,Tang W,Cao H,Wang X,Chen Z




Has Abstract


2020-02-10 00:00:00














  • Macromitophagy is a longevity assurance process that in chronologically aging yeast limited in calorie supply sustains functional mitochondria and maintains cellular lipid homeostasis.

    abstract::Macromitophagy controls mitochondrial quality and quantity. It involves the sequestration of dysfunctional or excessive mitochondria within double-membrane autophagosomes, which then fuse with the vacuole/lysosome to deliver these mitochondria for degradation. To investigate a physiological role of macromitophagy in y...


    pub_type: 杂志文章


    authors: Richard VR,Leonov A,Beach A,Burstein MT,Koupaki O,Gomez-Perez A,Levy S,Pluska L,Mattie S,Rafesh R,Iouk T,Sheibani S,Greenwood M,Vali H,Titorenko VI

    更新日期:2013-04-01 00:00:00

  • Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma.

    abstract::Head and neck squamous cell carcinoma (HNSCC), a major histologic subtype of head and neck cancer, presents great mortality and morbidity worldwide. The aim of this study is to discover new potential biomarkers closely correlated with HNSCC progression. In this study, weighted gene co-expression network analysis was a...


    pub_type: 杂志文章


    authors: Xu W,Zou H,Wei Z,Song C,Tang C,Yin X,Wang Y,Han S,Cai Y,Han W

    更新日期:2019-06-06 00:00:00

  • Validation of the 8th edition of the American Joint Committee on Cancer Pathological Prognostic Staging for young breast cancer patients.

    abstract:PURPOSE:This study aimed to validate the newly proposed American Joint Committee on Cancer (AJCC) pathological prognostic staging system for young breast cancer patients (aged ≤40 years). RESULTS:We included 12811 women in this study. Overall, 52.8% of patients in the 7th AJCC stages were restaged to the 8th AJCC path...


    pub_type: 杂志文章


    authors: Zhou J,Lei J,Wang J,Lian CL,Hua L,Yang LC,Wu SG

    更新日期:2020-04-22 00:00:00

  • Age-related deficits in skeletal muscle recovery following disuse are associated with neuromuscular junction instability and ER stress, not impaired protein synthesis.

    abstract::Age-related loss of muscle mass and strength can be accelerated by impaired recovery of muscle mass following a transient atrophic stimulus. The aim of this study was to identify the mechanisms underlying the attenuated recovery of muscle mass and strength in old rats following disuse-induced atrophy. Adult (9 month) ...


    pub_type: 杂志文章


    authors: Baehr LM,West DW,Marcotte G,Marshall AG,De Sousa LG,Baar K,Bodine SC

    更新日期:2016-01-01 00:00:00

  • The IL1β-HER2-CLDN18/CLDN4 axis mediates lung barrier damage in ARDS.

    abstract:OBJECTIVE:The high mortality rate associated with acute respiratory distress syndrome (ARDS) is a major challenge for intensive care units. In the present study, we applied bioinformatics and animal models to identify core genes and potential corresponding pathways in ARDS. RESULTS:Using bioinformatics analysis, IL-1β...


    pub_type: 杂志文章


    authors: Ma X,Yu X,Zhou Q

    更新日期:2020-02-15 00:00:00

  • Silencing KIF14 reverses acquired resistance to sorafenib in hepatocellular carcinoma.

    abstract::For nearly a decade, sorafenib has served as a first-line chemotherapeutic drug for the treatment of hepatocellular carcinoma (HCC), but it displays only limited efficacy against advanced drug-resistant HCC. Regorafenib, the first second-line drug approved for treatment after sorafenib failure, can reverse resistance ...


    pub_type: 杂志文章


    authors: Zhu Q,Ren H,Li X,Qian B,Fan S,Hu F,Xu L,Zhai B

    更新日期:2020-11-16 00:00:00

  • Identification of a hippocampal lncRNA-regulating network in cognitive dysfunction caused by chronic cerebral hypoperfusion.

    abstract::Cognitive dysfunction caused by chronic cerebral hypoperfusion is a common underlying cause of many cognition-related neurodegenerative diseases. The mechanisms of cognitive dysfunction caused by CCH are not clear. Long non-coding RNA is involved in synaptic plasticity and cognitive function, but whether lncRNA is inv...


    pub_type: 杂志文章


    authors: Yao ZH,Wang J,Shen BZ,Li YT,Yao XL,Zhang SF,Zhang Y,Hu JC,Xie YC

    更新日期:2020-10-11 00:00:00

  • Retinoic acid treated human dendritic cells induce T regulatory cells via the expression of CD141 and GARP which is impaired with age.

    abstract::Aged subjects display increased susceptibility to mucosal diseases. Retinoic Acid (RA) plays a major role in inducing tolerance in the mucosa. RA acts on Dendritic cells (DCs) to induce mucosal tolerance. Here we compared the response of DCs from aged and young individuals to RA with a view to understand the role of D...


    pub_type: 杂志文章


    authors: Agrawal S,Ganguly S,Tran A,Sundaram P,Agrawal A

    更新日期:2016-06-01 00:00:00

  • MiR-191 inhibit angiogenesis after acute ischemic stroke targeting VEZF1.

    abstract::Acute ischemic stroke (AIS) is a major public health problem in China. Impaired angiogenesis plays crucial roles in the development of ischemic cerebral injury. Recent studies have identified that microRNAs (miRNAs) are important regulators of angiogenesis, but little is known the exact effects of angiogenesis-associa...


    pub_type: 杂志文章


    authors: Du K,Zhao C,Wang L,Wang Y,Zhang KZ,Shen XY,Sun HX,Gao W,Lu X

    更新日期:2019-05-07 00:00:00

  • Impact papers on aging in 2009.

    abstract::The Editorial Board of Aging reviews research papers published in 2009, which they believe have or will have significant impact on aging research. Among many others, the topics include genes that accelerate aging or in contrast promote longevity in model organisms, DNA damage responses and telomeres, molecular mechani...


    pub_type: 杂志文章,评审


    authors: Blagosklonny MV,Campisi J,Sinclair DA,Bartke A,Blasco MA,Bonner WM,Bohr VA,Brosh RM Jr,Brunet A,Depinho RA,Donehower LA,Finch CE,Finkel T,Gorospe M,Gudkov AV,Hall MN,Hekimi S,Helfand SL,Karlseder J,Kenyon C,Kroeme

    更新日期:2010-03-01 00:00:00

  • Resistin enhances angiogenesis in osteosarcoma via the MAPK signaling pathway.

    abstract::Over the last two decades, there have been no significant changes in patient outcomes in relation to the treatment of osteosarcoma, an aggressive malignant neoplasm. It is known that vascular endothelial growth factor-A (VEGF-A) plays a crucial role in angiogenesis and in osteosarcoma. Moreover, VEGF-A expression corr...


    pub_type: 杂志文章


    authors: Tsai HC,Cheng SP,Han CK,Huang YL,Wang SW,Lee JJ,Lai CT,Fong YC,Tang CH

    更新日期:2019-11-13 00:00:00

  • Annotation of susceptibility SNPs associated with atrial fibrillation.

    abstract:OBJECTIVE:Genome-wide association studies (GWAS) and the candidate gene based association studies have identified a panel of variants associated with atrial fibrillation (AF), however, most of the identified single nucleotide polymorphisms (SNPs) were found located within intergenic or intronic genomic regions, and whe...


    pub_type: 杂志文章


    authors: Xu C,Zhang R,Xia Y,Xiong L,Yang W,Wang P

    更新日期:2020-09-09 00:00:00

  • Hypothalamic ΔFosB prevents age-related metabolic decline and functions via SNS.

    abstract::The ventral hypothalamus (VHT) integrates several physiological cues to maintain glucose homeostasis and energy balance. Aging is associated with increased glucose intolerance but the underlying mechanisms responsible for age-related metabolic decline, including neuronal signaling in the VHT, remain elusive. We have s...


    pub_type: 杂志文章


    authors: Sato K,Idelevich A,Nagano K,Rowe GC,Gori F,Baron R

    更新日期:2017-01-20 00:00:00

  • Histological characteristics of bone in-growth of proximal humeral implants with different spatial structures.

    abstract::This study compares the longitudinal histological characteristics of proximal humeral implants with different spatial structures in rabbits. Thirty skeletally-mature male rabbits were divided into a trabecular structure group and regular hexahedron structure group according to the different spatial structures of a bio...


    pub_type: 杂志文章


    authors: Xue Z,Pei Z,Zhang H,Tang C,Jia J,Zhang K,Zhang K,Wang L,Guan Z

    更新日期:2021-01-20 00:00:00

  • CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age.

    abstract::Natural killer cells lacking expression of CD56 (CD56neg NK cells) have been described in chronic HIV and hepatitis C virus infection. Features and functions of CD56neg NK cells in the context of latent infection with CMV and / or EBV with age are not known. In a cohort of healthy donors >60 years of age, we found tha...


    pub_type: 杂志文章


    authors: Müller-Durovic B,Grählert J,Devine OP,Akbar AN,Hess C

    更新日期:2019-01-27 00:00:00

  • Osteoprotegerin as a biomarker of geriatric frailty syndrome.

    abstract::The lack of a univocal definition of frailty, a condition frequently found in the elderly population which is correlated with an increased risk of mortality, has prompted the search for clinical and laboratory parameters associated with this condition. Whereas OPG is a protein involved in different pathophysiological ...


    pub_type: 杂志文章


    authors: Valentini A,Cianfarani MA,Tarantino U,Di Daniele N,Bertoli A

    更新日期:2019-07-17 00:00:00

  • Periodontitis in elderly patients with type 2 diabetes mellitus: impact on gut microbiota and systemic inflammation.

    abstract::Elderly patients with type 2 diabetes mellitus (T2DM) exhibit considerable periodontitis frequency, which causes tooth loss and poor quality of life. To investigate the impact of periodontitis on gut microbiota, we used 16S rRNA amplicon sequencing to characterize the composition and structure of gut microbiota among ...


    pub_type: 杂志文章


    authors: Li J,Lu H,Wu H,Huang S,Chen L,Gui Q,Zhou W,Yang Y,Wu Y,Zhang H,Zhang Q,Yang Y

    更新日期:2020-11-25 00:00:00

  • Prognostic value of epithelial-mesenchymal transition markers in clear cell renal cell carcinoma.

    abstract::Epithelial-to-mesenchymal transition (EMT) is important in tumor invasiveness and metastasis. We aimed to determine prognostic value of six key EMT markers (CDH1, CDH2, SNAI1, SNAI2, VIM, TWIST1) in clear cell renal cell carcinoma (ccRCC). A total of 533 ccRCC patients with RNASeq data from The Cancer Genome Atlas (TC...


    pub_type: 杂志文章


    authors: Xu H,Xu WH,Ren F,Wang J,Wang HK,Cao DL,Shi GH,Qu YY,Zhang HL,Ye DW

    更新日期:2020-01-08 00:00:00

  • Risk factors and prediction of second primary cancer in primary female non-metastatic breast cancer survivors.

    abstract::This study aimed to investigate the risk factors of second primary cancer among female breast cancer (BC) survivors, with emphasis on the prediction of the individual risk conditioned on the patient's characteristics. We identified 208,474 BC patients diagnosed between 2004 and 2010 from the Surveillance, Epidemiology...


    pub_type: 杂志文章


    authors: Qian X,Jia H,Zhang Y,Ma B,Qin G,Wu Z

    更新日期:2020-10-13 00:00:00

  • A single-nucleotide polymorphism (rs1805087) in the methionine synthase (METH) gene increases the risk of prostate cancer.

    abstract::Methionine synthase (METH, i.e., MTR) is a key enzyme in the folate pathway, which plays a critical role in the synthesis, repair, and methylation of DNA. The association between METH gene polymorphisms and prostate cancer susceptibility remains ambiguous. Thus, we performed an updated meta-analysis of METH single-nuc...


    pub_type: 杂志文章,meta分析,评审


    authors: Zhang X,Tang J,Shen N,Ren K

    更新日期:2018-10-18 00:00:00

  • G-protein coupled estrogen receptor activation protects the viability of hyperoxia-treated primary murine retinal microglia by reducing ER stress.

    abstract::In this study, we investigated the effects of G-protein coupled estrogen receptor (GPER) activation in the early phase of retinopathy of prematurity (ROP) and its association with endoplasmic reticulum (ER) stress using primary murine retinal microglia as an experimental model. Fluorescence microscopy results show tha...


    pub_type: 杂志文章


    authors: Li R,Wang Y,Chen P,Meng J,Zhang H

    更新日期:2020-09-13 00:00:00

  • Cohort profile: the Diet and Healthy Aging (DaHA) study in Singapore.

    abstract::How diet is related with cognition and health has not been systematically examined in Asians whose eating habits are very different from their counterparts in the West and the biological mechanisms underlying such links are not well known yet. The diet and healthy aging (DaHA) study is a community-based longitudinal s...


    pub_type: 杂志文章


    authors: Yu R,Sun Y,Ye KX,Feng Q,Lim SL,Mahendran R,Cheah IK,Foo RSY,Chua RY,Gwee X,Loh M,Sarmugam R,Khine WWT,Chao YX,Larbi A,Lee YK,Kumar AP,Kennedy BK,Kua EH,Feng L

    更新日期:2020-11-18 00:00:00

  • One-carbon metabolism: an aging-cancer crossroad for the gerosuppressant metformin.

    abstract::The gerosuppressant metformin operates as an efficient inhibitor of the mTOR/S6K1 gerogenic pathway due to its ability to ultimately activate the energy-sensor AMPK. If an aging-related decline in the AMPK sensitivity to cellular stress is a crucial event for mTOR-driven aging and aging-related diseases, including can...


    pub_type: 杂志文章


    authors: Menendez JA,Joven J

    更新日期:2012-12-01 00:00:00

  • Gender- and region-specific changes in estrogen signaling in aging rat brain mitochondria.

    abstract::Recently epidemiological studies suggest females lose neuroprotection from neurodegenerative diseases as they go through menopause. It has been hypothesized that this neuroprotection is hormone-dependent. The current study characterized cell signaling molecules downstream of estrogen receptor beta that are known to pl...


    pub_type: 杂志文章


    authors: Evola CM,Hudson TL,Huang L,Corbett AM,Mayes DA

    更新日期:2018-08-31 00:00:00

  • Association between serum levels of Klotho and inflammatory cytokines in cardiovascular disease: a case-control study.

    abstract::Decrease in soluble anti-aging Klotho protein levels is associated to cardiovascular disease (CVD). Diverse studies have shown a bidirectional relationship between Klotho and inflammation, a risk factor for the development of CVD. In this work we aimed to evaluate the association between Klotho and inflammatory cytoki...


    pub_type: 杂志文章


    authors: Martín-Núñez E,Donate-Correa J,Ferri C,López-Castillo Á,Delgado-Molinos A,Hernández-Carballo C,Pérez-Delgado N,Rodríguez-Ramos S,Cerro-López P,Tagua VG,Mora-Fernández C,Navarro-González JF

    更新日期:2020-01-27 00:00:00

  • Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer.

    abstract::Despite improvements in surgical procedures and comprehensive therapies, pancreatic cancer remains one of the most aggressive and deadly human malignancies. It is therefore necessary to determine which cellular mediators associate with prognosis in pancreatic cancer so as to improve the treatment of this disease. In t...


    pub_type: 杂志文章


    authors: Li Y,Kong R,Chen H,Zhao Z,Li L,Li J,Hu J,Zhang G,Pan S,Wang Y,Wang G,Chen H,Sun B

    更新日期:2019-07-21 00:00:00

  • Identification of survival-associated alternative splicing events and signatures in adrenocortical carcinoma based on TCGA SpliceSeq data.

    abstract:OBJECTIVE:To explore the correlations among alternative splicing (AS), splicing factors (SF) and survival outcome in adrenocortical carcinoma (ACC) patients. RESULTS:A total of 92 ACC patients were included. Univariate analysis identified 3919 AS events significantly associated with overall survival. Lasso method foll...


    pub_type: 杂志文章


    authors: Xu N,Ke ZB,Lin XD,Lin F,Chen SH,Wu YP,Chen YH,Wei Y,Zheng QS

    更新日期:2020-03-26 00:00:00

  • Immune senescence and immune activation in elderly colorectal cancer patients.

    abstract::In our previous study, we found that low thymic output and short telomere length were associated with a higher risk of tumor in elderly cancer patients. Here, we aimed to examine in depth the impact of immunological and biological senescence and immune activation on disease outcome in elderly patients with colorectal ...


    pub_type: 杂志文章


    authors: Giunco S,Petrara MR,Bergamo F,Del Bianco P,Zanchetta M,Carmona F,Zagonel V,De Rossi A,Lonardi S

    更新日期:2019-06-13 00:00:00

  • TIP-B1 promotes kidney clear cell carcinoma growth and metastasis via EGFR/AKT signaling.

    abstract::Kidney clear cell carcinoma (KIRC) is the most prevalent kidney malignancy. Accumulating evidence shows that high expression of TIP-B1 correlates with development of tumor progression. However, the detailed functions of TIP-B1 in the KIRC remain to be further elucidated. Here, we firstly found TIP-B1 expression was si...


    pub_type: 杂志文章


    authors: Yin L,Gao S,Shi H,Wang K,Yang H,Peng B

    更新日期:2019-09-27 00:00:00

  • Cross-platform genomic identification and clinical validation of breast cancer diagnostic biomarkers.

    abstract:INTRODUCTION:Circulating non-coding RNA is an ideal source to discover novel biomarkers for non-invasive screening. However, studies for the discovery of universal miRNAs in serum and exosomes for breast cancer early diagnosis are limited. METHODS:Based on bioinformatic analysis, in vitro and in vivo studies were perf...


    pub_type: 杂志文章


    authors: Liu D,Li B,Shi X,Zhang J,Chen AM,Xu J,Wang W,Huang K,Gao J,Zheng Z,Liu D,Wang H,Shi W,Chen L,Xu J

    更新日期:2021-01-20 00:00:00