Abstract:
:Intracortical electrodes for brain-machine interfaces rely on intimate contact with tissues for recording signals and stimulating neurons. However, the long-term viability of intracortical electrodes in vivo is poor, with a major contributing factor being the development of a glial scar. In vivo approaches for evaluating responses to intracortical devices are resource intensive and complex, making statistically significant, high throughput data difficult to obtain. In vitro models provide an alternative to in vivo studies; however, existing approaches have limitations which restrict the translation of the cellular reactions to the implant scenario. Notably, there is no current robust model that includes astrocytes, microglia, oligodendrocytes and neurons, the four principle cell types, critical to the health, function and wound responses of the central nervous system (CNS). In previous research a co-culture of primary mouse mature mixed glial cells and immature neural precursor cells were shown to mimic several key properties of the CNS response to implanted electrode materials. However, the method was not robust and took up to 63 days, significantly affecting reproducibility and widespread use for assessing brain-material interactions. In the current research a new co-culture approach has been developed and evaluated using immunocytochemistry and quantitative polymerase chain reaction (qPCR). The resulting method reduced the time in culture significantly and the culture model was shown to have a genetic signature similar to that of healthy adult mouse brain. This new robust CNS culture model has the potential to significantly improve the capacity to translate in vitro data to the in vivo responses.
journal_name
Front Neuroscijournal_title
Frontiers in neuroscienceauthors
Gilmour A,Poole-Warren L,Green RAdoi
10.3389/fnins.2019.01349subject
Has Abstractpub_date
2019-12-17 00:00:00pages
1349eissn
1662-4548issn
1662-453Xjournal_volume
13pub_type
杂志文章abstract::Recently, Parkinson's disease-associated α-synuclein (αS) has emerged as an important regulator for SNARE-dependent vesicle fusion. However, it is controversial if excessive accumulation of αS, even in the absence of aggregation, impairs neurotransmission. Here we use a single vesicle fusion assay with ms time resolut...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2019.00216
更新日期:2019-03-21 00:00:00
abstract::The aim of our study was to evaluate the effects of chronic administration of nandrolone-decanoate (ND) or testosterone-enanthate (TE) in supraphysiological doses and a prolonged swimming protocol, alone and in combination with ND or TE, on anxiety-like behavior in rats. We investigated the immunohistochemical alterat...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2019.00172
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abstract::Environmental sounds form part of our daily life. With the advancement of deep learning models and the abundance of training data, the performance of automatic sound classification (ASC) systems has improved significantly in recent years. However, the high computational cost, hence high power consumption, remains a ma...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
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journal_title:Frontiers in neuroscience
pub_type: 杂志文章,评审
doi:10.3389/fnins.2020.00589
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abstract::Sensory loss involves irreversible behavioral and neural changes. Paradigms of short-term limb immobilization mimic deprivation of proprioceptive inputs and motor commands, which occur after the loss of limb use. While several studies have shown that short-term immobilization induced motor control impairments, the ori...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.00696
更新日期:2020-07-01 00:00:00
abstract::Several works have demonstrated that visual experience plays a critical role in the development of allocentric spatial coding. Indeed, while children with a typical development start to code space by relying on allocentric landmarks from the first year of life, blind children remain anchored to an egocentric perspecti...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.00565
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abstract::We describe the steps involved in analysis of multi-modal, multi-subject human neuroimaging data using the SPM12 free and open source software (https://www.fil.ion.ucl.ac.uk/spm/) and a publically-available dataset organized according to the Brain Imaging Data Structure (BIDS) format (https://openneuro.org/datasets/ds...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2019.00300
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abstract::Peripheral nerves are composed of complex layered anatomical structures, including epineurium, perineurium, and endoneurium. Perineurium and endoneurium contain many physical barriers, including the blood-nerve barrier at endoneurial vessels and the perineurial barrier. These physical barriers help to eliminate flux p...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章,评审
doi:10.3389/fnins.2018.00597
更新日期:2018-08-23 00:00:00
abstract::The mean diffusivity (MD) value has been used to describe microstructural properties in Diffusion Tensor Imaging (DTI) in cortical gray matter (GM). Recently, researchers have applied a cortical surface generated from the T1-weighted volume. When the DTI data are analyzed using the cortical surface, it is important to...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
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journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2010.00300
更新日期:2010-09-15 00:00:00
abstract::Frontotemporal Dementia (FTD) is a focal neurodegenerative disease, with a strong genetic background, that causes early onset dementia. The present knowledge about the risk loci and causative mutations of FTD mainly derives from genetic linkage analysis, studies of candidate genes, Genome-Wide Association Studies (GWA...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章,评审
doi:10.3389/fnins.2019.00506
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abstract::Although flight is regarded as a key behavior of birds this review argues that the perceptual demands for its control are met within constraints set by the perceptual demands of two other key tasks: the control of bill (or feet) position, and the detection of food items/predators. Control of bill position, or of the f...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章,评审
doi:10.3389/fnins.2017.00619
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abstract::This work presents sPyNNaker 4.0.0, the latest version of the software package for simulating PyNN-defined spiking neural networks (SNNs) on the SpiNNaker neuromorphic platform. Operations underpinning realtime SNN execution are presented, including an event-based operating system facilitating efficient time-driven ne...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2018.00816
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abstract::D-norpseudoephedrine (NPE), also known as cathine, is found naturally in the shrub Catha edulis "Khat." NPE has been widely used as an appetite suppressant for the treatment of obesity. Although it is known that NPE acts on α1-adrenergic receptors, there is little information about the role of dopamine receptors on NP...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.572328
更新日期:2020-10-16 00:00:00
abstract::Environmental stressors including protein malnutrition (PMN) during pre-, neo- and post-natal age have been documented to affect cognitive development and cause increased susceptibility to neuropsychiatric disorders. Most studies have addressed either of the three windows and that does not emulate the clinical conditi...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2015.00446
更新日期:2015-12-08 00:00:00
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journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2016.00271
更新日期:2016-06-14 00:00:00
abstract::EEG-based Brain-Computer Interfaces (BCIs) are becoming a new tool for neurorehabilitation. BCIs are used to help stroke patients to improve the functional capability of the impaired limbs, and to communicate and assess the level of consciousness in Disorder of Consciousness (DoC) patients. BCIs based on a motor image...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2018.00308
更新日期:2018-05-11 00:00:00
abstract::Traditionally, the visual motion area (MT) is considered as a brain region specialized for visual motion perception. However, accumulating evidence showed that MT is also related to various functions, suggesting that it is a complex functional area and different functional subregions might exist in this area. To delin...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.00427
更新日期:2020-05-27 00:00:00
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journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2016.00216
更新日期:2016-05-12 00:00:00
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journal_title:Frontiers in neuroscience
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doi:10.3389/fnins.2016.00109
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journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.00172
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journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2014.00333
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journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.00568
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journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.00477
更新日期:2020-05-28 00:00:00
abstract:Objective:To evaluate the effects of long-term High-definition transcranial direct current stimulation (HD-tDCS) over precuneus on the level of consciousness (LOC) and the relationship between Mismatch negativity (MMN) and the LOC over the therapy period in patients with Disorders of consciousness (DOCs). Methods:We e...
journal_title:Frontiers in neuroscience
pub_type: 杂志文章
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更新日期:2020-04-28 00:00:00
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journal_title:Frontiers in neuroscience
pub_type: 杂志文章
doi:10.3389/fnins.2020.563768
更新日期:2020-11-03 00:00:00