Abstract:
:Rationale: Spatial-temporal control of cell fate in vivo is of great importance for regenerative medicine. Currently, there remain no practical strategies to tune cell-fate spatial-temporally. Optogenetics is a biological technique that widely used to control cell activity in genetically defined neurons in a spatiotemporal-specific manner by light. In this study, optogenetics was repurposed for precise bone tissue regeneration. Methods: Lhx8 and BMP2 genes, which are considered as the master genes for mesenchymal stem cell proliferation and differentiation respectively, were recombined into a customized optogenetic control system. In the system, Lhx8 was constitutively expressed, while BMP2 together with shLhx8 expression was driven by blue light. Results: As expected, blue light induced BMP2 expression and inactivated Lhx8 expression in cells infected with the optogenetic control system. Optogenetic control of BMP2 and Lhx8 expression inversely regulates MSC fate in vitro. By animal study, we found that blue light could fine-tune the regeneration in vivo. Blue light illumination significantly promotes bone regeneration when the scaffold was loaded with MSCs infected with adeno-Lhx8, GI-Gal4DBD, LOV-VP16, and BMP2-shLhx8. Conclusions: Together, our study revealed that optogenetic control of the master genes for mesenchymal stem cell proliferation and differentiation would be such a candidate strategy for precise regenerative medicine.
journal_name
Theranosticsjournal_title
Theranosticsauthors
Wang W,Huang D,Ren J,Li R,Feng Z,Guan C,Bao B,Cai B,Ling J,Zhou Cdoi
10.7150/thno.36455subject
Has Abstractpub_date
2019-10-18 00:00:00pages
8196-8205issue
26issn
1838-7640pii
thnov09p8196journal_volume
9pub_type
杂志文章相关文献
Theranostics文献大全abstract::CUB-domain containing protein 1 (CDCP1) is a cancer associated cell surface protein that amplifies pro-tumorigenic signalling by other receptors including EGFR and HER2. Its potential as a cancer target is supported by studies showing that anti-CDCP1 antibodies inhibit cell migration and survival in vitro, and tumor g...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.30736
更新日期:2020-01-12 00:00:00
abstract::We report a new technique to detect enzyme activity inside cells. The method based on Fluorescence Lifetime Imaging (FLIM) technology allows one to follow sensor cleavage by proteolytic enzyme caspase-3. Specifically, we use the FLIM FRET of living cells via the confocal fluorescence microscopy. A specially designed l...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.3885
更新日期:2012-01-01 00:00:00
abstract::Autophagy is a dynamic and complicated catabolic process. Imaging autophagic flux can clearly advance knowledge of its pathophysiology significance. While the most common way autophagy is imaged relies on fluorescent protein-based probes, this method requires substantial genetic manipulation that severely restricts th...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.33867
更新日期:2019-08-01 00:00:00
abstract::Gastroenteropancreatic neuroendocrine neoplasms are heterogeneous in their clinical behavior and require therapies specially tailored according to staging, grading, origin and expression of peptide receptors. Despite extensive scientific efforts, the therapy options are still not satisfactory. The main reasons are due...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.7851
更新日期:2014-01-29 00:00:00
abstract::Background: Tumor cells require proficient autophagy to meet high metabolic demands and resist chemotherapy, which suggests that reducing autophagic flux might be an attractive route for cancer therapy. However, this theory in clinical cancer research remains controversial due to the limited number of drugs that speci...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.22012
更新日期:2018-01-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) have emerged as integral components of E2F1-regulated gene regulatory networks (GRNs), but their implication in advanced or treatment-refractory malignancy is unknown. Methods: We combined high-throughput transcriptomic approaches with bioinformatics and structure modeling to search for ...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44176
更新日期:2020-07-29 00:00:00
abstract::Lung cancer is the leading cause of cancer-related death worldwide. Most targeted drugs approved for lung cancer treatment are tyrosine kinase inhibitors (TKIs) directed against EGFR or ALK, and are used mainly for adenocarcinoma. At present, there is no effective or tailored targeting agent for large cell carcinoma (...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.17573
更新日期:2017-04-10 00:00:00
abstract::This article aims at alerting scientists working with nanoparticles or microparticles about the specific adverse reactions due to the intravascular pulmonary macrophages present in pigs and other cloven-hoof animals, but not in humans. The history of a 25-year old study of an ultrasound contrast agent is used to illus...
journal_title:Theranostics
pub_type: 评论,杂志文章
doi:10.7150/thno.22420
更新日期:2017-10-23 00:00:00
abstract::Gold nanoparticles (AuNPs) have been reported to promote osteogenic differentiation of mesenchymal stem cells and osteoblasts, but little is known about their effects on human periodontal ligament progenitor cells (PDLPs). In this study, we evaluated the effects of AuNPs with various diameters (5, 13 and 45 nm) on the...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.17252
更新日期:2017-03-06 00:00:00
abstract::Rationale: As the central hallmark of liver fibrosis, transdifferentiation of hepatic stellate cells (HSCs), the predominant contributor to fibrogenic hepatic myofibroblast responsible for extracellular matrix (ECM) deposition, is characterized with transcriptional and epigenetic remodeling. We aimed to characterize t...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.46360
更新日期:2021-01-01 00:00:00
abstract::Rationale: Increasing frequency of human exposure to PEG-related products means that healthy people are likely to have pre-existing anti-PEG antibodies (pre-αPEG Ab). However, the influence of pre-αPEG Abs on the pharmacokinetics (PK) and therapeutic efficacy of LipoDox is unknown. Methods: We generated two pre-αPEG A...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.22164
更新日期:2018-05-09 00:00:00
abstract::Natural polysaccharides have received a lot of attention in the biomedical field. Indeed, sources of polysaccharides, extracted or produced from plants, bacteria, fungi or algae, are diverse and renewable. Moreover, recent progresses in polysaccharide chemistry and nanotechnologies allow elaborating new dedicated nano...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.7688
更新日期:2014-03-11 00:00:00
abstract::Sepsis is a major cause of patient mortality and morbidity from bacterial infections. Although neutrophils are known to be important in the development of sepsis, how distinctive neutrophil subtypes regulate inflammatory processes involved in septicemia remains unclear. Preconditioning protects organisms against subse...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.37139
更新日期:2019-08-14 00:00:00
abstract::Successful anticancer chemotherapy requires targeting tumors efficiently and further potential to eliminate cancer stem cell (CSC) subpopulations. Since CD44 is present on many types of CSCs, and it binds specially to hyaluronic acid (HA), we tested whether coating solid lipid nanoparticles with hyaluronan (HA-SLNs)wo...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.10804
更新日期:2015-04-05 00:00:00
abstract::The intestinal exporter MRP2 plays an important role in disposition and elimination of a wide range of drugs. Here, we aimed to clarify the impact of circadian clock on intestinal MRP2, and to determine the molecular mechanisms for generation of diurnal MRP2 expression. Methods: The regulatory effects of Bmal1 on inte...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.33395
更新日期:2019-04-13 00:00:00
abstract::Objective: Vascular smooth muscle cells (VSMCs) undergo the phenotypic changes from contractile to synthetic state during vascular remodeling after ischemia. SIRT1 protects against stress-induced vascular remodeling via maintaining VSMC differentiated phenotype. However, the effect of smooth muscle SIRT1 on the functi...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.39320
更新日期:2020-01-01 00:00:00
abstract::The most frequent malignancy of the pancreas is the pancreatic ductal adenocarcinoma (PDAC). Despite many efforts PDAC has still a dismal prognosis. Biomarkers for early disease stage diagnosis as a prerequisite for a potentially curative treatment are still missing. Novel blood-based markers may help to overcome this...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.29247
更新日期:2019-02-12 00:00:00
abstract::Background: Programmed cell death protein 1 (PD1) inhibitors have revolutionized cancer therapy, yet many patients fail to respond. Thus, the identification of accurate predictive biomarkers of therapy response will improve the clinical benefit of anti-PD1 therapy. Method: We assessed the baseline serological autoanti...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45816
更新日期:2020-05-16 00:00:00
abstract::Cerebrospinal fluid (CSF) leakage can lead to brain and spine pathologies and there is an urgent need for a rapid diagnostic method for determining CSF leakage. Beta-2 transferrin (β2TF), asialotransferrin, is a specific CSF glycoprotein biomarker used to determine CSF leakage when distinguished from serum sialotransf...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.34411
更新日期:2019-05-31 00:00:00
abstract::Conventional immunosuppressants cause side effects and do not prevent the recurrence of autoimmune diseases. Moreover, they may not inhibit autoimmunity mediated by pathogenic memory T-cells. Dihydroartemisinin (DHA) has been shown to regulate autoimmunity. However, it remains unknown whether DHA impacts psoriasis and...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45211
更新日期:2020-08-21 00:00:00
abstract::The therapeutic efficacy of fluorescence image-guided tumor surgery and photodynamic therapy (PDT) is impaired by the penetration depth limitation, low signal-to-noise ratio of traditional first near-infrared window (NIR I) fluorescence and the hypoxic tumor microenvironment. Here, a "red blood cell-based multimodal p...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.29817
更新日期:2019-01-01 00:00:00
abstract::Photodynamic therapy (PDT) is an emerging theranostic modality for various cancers and diseases. The focus of this study was the development of tumor-targeting albumin nanoparticles containing photosensitizers for efficient PDT. To produce tumor-targeting albumin nanoparticles, the hydrophobic photosensitizer, chlorin...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno/v01p0230
更新日期:2011-04-06 00:00:00
abstract::Using non-covalent interactions between nucleic acids (DNA, siRNA, miRNA, and mRNA) with peptides or nucleopeptides is a promising strategy to construct supramolecular assemblies for gene delivery and therapy. Comparing to conventional strategies for gene delivery, the assemblies of peptides or nucleopeptides provide ...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.31854
更新日期:2019-05-18 00:00:00
abstract::Heart diseases are one of the leading causes of death for humans in the world. Increasing evidence has shown that myocardial injury induced innate and adaptive immune responses upon early cellular damage but also during chronic phases post-injury. The immune cells can not only aggravate the injury but also play an ess...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.33112
更新日期:2019-05-31 00:00:00
abstract::5-Fluorouracil (5-FU) combined with radiotherapy is a common treatment strategy to treat human cancers, but the underlying mechanisms of this combination treatment remain unclear. Here, we report that NAD+-dependent deacetylase sirtuin-7 (SIRT7) protein levels were decreased due to 5-FU exposure rendering colorectal c...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.18804
更新日期:2017-03-22 00:00:00
abstract::[This corrects the article DOI: 10.7150/thno.27882.]. ...
journal_title:Theranostics
pub_type: 杂志文章,已发布勘误
doi:10.7150/thno.49958
更新日期:2020-07-10 00:00:00
abstract::Rationale: Following an ever-increased focus on personalized medicine, there is a continuing need to develop preclinical molecular imaging modalities to guide the development and optimization of targeted therapies. Near-Infrared (NIR) Macroscopic Fluorescence Lifetime Förster Resonance Energy Transfer (MFLI-FRET) imag...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.45825
更新日期:2020-08-15 00:00:00
abstract::Small interfering RNA (siRNA) has proved to be a powerful tool for target-specific gene silencing via RNA interference (RNAi). Its ability to control targeted gene expression gives new hope to gene therapy as a treatment for cancers and genetic diseases. However, siRNA shows poor pharmacological properties, such as lo...
journal_title:Theranostics
pub_type: 杂志文章,评审
doi:10.7150/thno.8491
更新日期:2014-09-19 00:00:00
abstract::The ability of brain tissue to regenerate is limited; therefore, brain diseases (i.e., trauma, stroke, tumors) often lead to irreversible motor and cognitive impairments. Therapeutic interventions using various types of injectable biomaterials have been investigated to promote endogenous neural differentiation. Despit...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.35606
更新日期:2019-05-31 00:00:00
abstract::Great progress has been made in the field of tumor immunotherapy in the past decade. However, the therapeutic effects of immune checkpoint blockade (ICB) against ovarian cancer are still limited. Recently, an inhibitor of cyclin-dependent kinases 4 and 6 (CDK4/6i) has been reported to enhance antitumor immunity in pre...
journal_title:Theranostics
pub_type: 杂志文章
doi:10.7150/thno.44871
更新日期:2020-08-25 00:00:00