Single enzyme loaded nanoparticles for combinational ultrasound-guided focused ultrasound ablation and hypoxia-relieved chemotherapy.

Abstract:

:Constructing nanosystems that synergistically combine therapeutic and diagnostic features is of great interest to the nanomedicine community but also remains a tremendous challenge. Methods: In this work, we report novel catalytic nanoparticles composed of the enzyme catalase, encapsulated in a polymer shell and surface decorated with pH-sensitive poly(ethylene glycol) (PEGylated nCAT). These nanoparticles were used as a promoter for ultrasound (US)-guided focused ultrasound (FUS) ablation and hypoxia alleviation for application in Doxorubicin-based chemotherapy. Results: The PEGylated nCAT produced highly effectively O2 from endogenous H2O2 to ameliorate the hypoxic and therefore poor-acoustic tumor environment. The generated O2 was utilized as 1) a contrast agent for US imaging; 2) strengthening agent for FUS ablation and 3) normoxia inducer to enhance chemotherapeutic efficacy. The PEGylated nCAT exhibited favorable enzyme activity after long-term storage, and after exposure to proteolytic conditions and elevated temperatures. The pH-responsive PEGylation contributed on the one hand to an extended in vivo circulation time over 48 h and on the other hand enabled PEG cleavage in the vicinity of cancer cells to facilitate cellular uptake. Conclusion: The developed PEGylated nCAT can therefore effectively combine US-guided FUS and chemotherapy and can be regarded as a highly promising theranostic platform.

journal_name

Theranostics

journal_title

Theranostics

authors

Zhu J,Li Z,Zhang C,Lin L,Cao S,Che H,Shi X,Wang H,van Hest JCM

doi

10.7150/thno.37054

subject

Has Abstract

pub_date

2019-10-17 00:00:00

pages

8048-8060

issue

26

issn

1838-7640

pii

thnov09p8048

journal_volume

9

pub_type

杂志文章
  • Chronic ethanol consumption and HBV induce abnormal lipid metabolism through HBx/SWELL1/arachidonic acid signaling and activate Tregs in HBV-Tg mice.

    abstract::Rationale: Chronic ethanol consumption as a public health problem worldwide boosts the development of chronic liver diseases in hepatitis B virus (HBV)-infected patients. Arachidonic acid metabolite prostaglandin E2 (PGE2) activates regulatory T cells (Tregs) function. Here, we aim to investigate the underlying mechan...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.46005

    authors: Liu Z,Wang J,Liu L,Yuan H,Bu Y,Feng J,Liu Y,Yang G,Zhao M,Yuan Y,Zhang H,Yun H,Zhang X

    更新日期:2020-07-23 00:00:00

  • A new class of PentixaFor- and PentixaTher-based theranostic agents with enhanced CXCR4-targeting efficiency.

    abstract::Non-invasive PET imaging of CXCR4 expression in cancer and inflammation as well as CXCR4-targeted radioligand therapy (RLT) have recently found their way into clinical research by the development of the theranostic agents [68Ga]PentixaFor (cyclo(D-Tyr1-D-[NMe]Orn2(AMBS-[68Ga]DOTA)-Arg3-Nal4-Gly5) = [68Ga]DOTA-AMBS-CPC...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.45537

    authors: Osl T,Schmidt A,Schwaiger M,Schottelius M,Wester HJ

    更新日期:2020-07-09 00:00:00

  • Absence of Interferon Regulatory Factor 1 Protects Against Atherosclerosis in Apolipoprotein E-Deficient Mice.

    abstract::Deciphering the molecular and cellular processes involved in foam cell formation is critical to understanding the pathogenesis of atherosclerosis. Interferon regulatory factor 1 (IRF1) was first identified as a transcriptional regulator of type-I interferons (IFNs) and IFN inducible genes. Our study aims to explore th...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.36862

    authors: Du M,Wang X,Mao X,Yang L,Huang K,Zhang F,Wang Y,Luo X,Wang C,Peng J,Liang M,Huang D,Huang K

    更新日期:2019-07-09 00:00:00

  • Labeling human mesenchymal stem cells with gold nanocages for in vitro and in vivo tracking by two-photon microscopy and photoacoustic microscopy.

    abstract::Stem cell tracking is a highly important subject. Current techniques based on nanoparticle-labeling, such as magnetic resonance imaging, fluorescence microscopy, and micro-computed tomography, are plagued by limitations including relatively low sensitivity or penetration depth, involvement of ionizing irradiation, and...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.5369

    authors: Zhang YS,Wang Y,Wang L,Wang Y,Cai X,Zhang C,Wang LV,Xia Y

    更新日期:2013-07-19 00:00:00

  • Activation of EP4 receptor limits transition of acute to chronic heart failure in lipoxygenase deficient mice.

    abstract::Aim: Immune responsive 12/15 lipoxygenase (12/15LOX)-orchestrate biosynthesis of essential inflammation-resolution mediators during acute inflammatory response in post-myocardial infarction (MI). Lack of 12/15LOX dampens proinflammatory mediator 12-(S)-hydroxyeicosatetraenoic acid (12-(S)-HETE), improves post-MI survi...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.51183

    authors: Kain V,Ingle KA,Rajasekaran NS,Halade GV

    更新日期:2021-01-01 00:00:00

  • Smart Sorting of Tumor Phenotype with Versatile Fluorescent Ag Nanoclusters by Sensing Specific Reactive Oxygen Species.

    abstract::Reactive oxygen species (ROS) play a crucial role in cancer formation and development, especially cancer metastasis. However, lack of a precise tool, which could accurately distinguish specific types of ROS, restricts an in-depth study of ROS in cancer development and progression. Herein, we designed smart and versati...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.38422

    authors: Chen X,Wang T,Le W,Huang X,Gao M,Chen Q,Xu S,Yin D,Fu Q,Shao C,Chen B,Shi D

    更新日期:2020-02-10 00:00:00

  • Response to mTOR and PI3K inhibitors in enzalutamide-resistant luminal androgen receptor triple-negative breast cancer patient-derived xenografts.

    abstract::Luminal androgen receptor (LAR) breast cancer accounts for 10% of all triple-negative breast cancers (TNBC). Anti-androgen therapy for this subtype is in development, but yields only partial clinical benefits. In this study, we aimed to characterize the genomic alterations of LAR TNBC, to analyze activation of the PI3...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.36182

    authors: Coussy F,Lavigne M,de Koning L,Botty RE,Nemati F,Naguez A,Bataillon G,Ouine B,Dahmani A,Montaudon E,Painsec P,Chateau-Joubert S,Laetitia F,Larcher T,Vacher S,Chemlali W,Briaux A,Melaabi S,Salomon AV,Guinebretiere JM

    更新日期:2020-01-01 00:00:00

  • Tumour Vascular Shutdown and Cell Death Following Ultrasound-Microbubble Enhanced Radiation Therapy.

    abstract::High-dose radiotherapy effects are regulated by acute tumour endothelial cell death followed by rapid tumour cell death instead of canonical DNA break damage. Pre-treatment with ultrasound-stimulated microbubbles (USMB) has enabled higher-dose radiation effects with conventional radiation doses. This study aimed to co...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.19010

    authors: El Kaffas A,Gangeh MJ,Farhat G,Tran WT,Hashim A,Giles A,Czarnota GJ

    更新日期:2018-01-01 00:00:00

  • Phototherapy-based combination strategies for bacterial infection treatment.

    abstract::The development of nanomedicine is expected to provide an innovative direction for addressing challenges associated with multidrug-resistant (MDR) bacteria. In the past decades, although nanotechnology-based phototherapy has been developed for antimicrobial treatment since it rarely causes bacterial resistance, the cl...

    journal_title:Theranostics

    pub_type: 杂志文章,评审

    doi:10.7150/thno.52729

    authors: Wei G,Yang G,Wang Y,Jiang H,Fu Y,Yue G,Ju R

    更新日期:2020-10-30 00:00:00

  • Erratum: pH-Sensitive Nano-Complexes Overcome Drug Resistance and Inhibit Metastasis of Breast Cancer by Silencing Akt Expression: Erratum.

    abstract::[This corrects the article DOI: 10.7150/thno.21516.]. ...

    journal_title:Theranostics

    pub_type: 已发布勘误

    doi:10.7150/thno.42847

    authors: Yin J,Lang T,Cun D,Zheng Z,Huang Y,Yin Q,Yu H,Li Y

    更新日期:2020-01-18 00:00:00

  • Pretargeted molecular imaging and radioimmunotherapy.

    abstract::Pretargeting is a multi-step process that first has an unlabeled bispecific antibody (bsMAb) localize within a tumor by virtue of its anti-tumor binding site(s) before administering a small, fast-clearing radiolabeled compound that then attaches to the other portion of the bsMAb. The compound's rapid clearance signifi...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.3582

    authors: Goldenberg DM,Chang CH,Rossi EA,J W,McBride,Sharkey RM

    更新日期:2012-01-01 00:00:00

  • 19q13 KRAB zinc-finger protein ZNF471 activates MAPK10/JNK3 signaling but is frequently silenced by promoter CpG methylation in esophageal cancer.

    abstract::Zinc-finger proteins (ZFPs) are the largest transcription factor family in mammals, involved in the regulation of multiple physiologic processes including cell differentiation, proliferation, apoptosis and neoplastic transformation. Approximately one-third of ZFPs are Krüppel-associated box domain (KRAB)-ZFPs. Methods...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.35861

    authors: Sun R,Xiang T,Tang J,Peng W,Luo J,Li L,Qiu Z,Tan Y,Ye L,Zhang M,Ren G,Tao Q

    更新日期:2020-01-12 00:00:00

  • Emerging role of mTOR in tumor immune contexture: Impact on chemokine-related immune cells migration.

    abstract::During the last few decades, cell-based anti-tumor immunotherapy emerged and it has provided us with a large amount of knowledge. Upon chemokines recognition, immune cells undergo rapid trafficking and activation in disease milieu, with immune cells chemotaxis being accompanied by activation of diverse intercellular s...

    journal_title:Theranostics

    pub_type: 杂志文章,评审

    doi:10.7150/thno.45219

    authors: Jin J,Zhao Q

    更新日期:2020-05-15 00:00:00

  • Biomechanically, structurally and functionally meticulously tailored polycaprolactone/silk fibroin scaffold for meniscus regeneration.

    abstract::Meniscus deficiency, the most common and refractory disease in human knee joints, often progresses to osteoarthritis (OA) due to abnormal biomechanical distribution and articular cartilage abrasion. However, due to its anisotropic spatial architecture, complex biomechanical microenvironment, and limited vascularity, m...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.44270

    authors: Li Z,Wu N,Cheng J,Sun M,Yang P,Zhao F,Zhang J,Duan X,Fu X,Zhang J,Hu X,Chen H,Ao Y

    更新日期:2020-04-06 00:00:00

  • Suppressing Sart1 to modulate macrophage polarization by siRNA-loaded liposomes: a promising therapeutic strategy for pulmonary fibrosis.

    abstract::Idiopathic pulmonary fibrosis (IPF) is a chronic and diffuse form of interstitial lung disease of unknown etiology with a fatal outcome. Although various strategies for IPF have been developed over the last few decades, no significant positive impact on the prognosis of IPF has been observed. According to the current ...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.48152

    authors: Pan T,Zhou Q,Miao K,Zhang L,Wu G,Yu J,Xu Y,Xiong W,Li Y,Wang Y

    更新日期:2021-01-01 00:00:00

  • SIRT6, a novel direct transcriptional target of FoxO3a, mediates colon cancer therapy.

    abstract::SIRT6, NAD+-dependent deacetylase sirtuin 6, has recently shown to suppress tumor growth in several types of cancer. Colon cancer is a challenging carcinoma associated with high morbidity and death. However, whether SIRT6 play a direct role in colon tumorigenesis and the underlying mechanism are not understood. Method...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.29724

    authors: Zhang Y,Nie L,Xu K,Fu Y,Zhong J,Gu K,Zhang L

    更新日期:2019-04-13 00:00:00

  • Dysregulated Kras/YY1/ZNF322A/Shh transcriptional axis enhances neo-angiogenesis to promote lung cancer progression.

    abstract::Angiogenesis enhances cancer metastasis and progression, however, the roles of transcription regulation in angiogenesis are not fully defined. ZNF322A is an oncogenic zinc-finger transcription factor. Here, we demonstrate a new mechanism of Kras mutation-driven ZNF322A transcriptional activation and elucidate the inte...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.47491

    authors: Lin CC,Kuo IY,Wu LT,Kuan WH,Liao SY,Jen J,Yang YE,Tang CW,Chen YR,Wang YC

    更新日期:2020-08-08 00:00:00

  • General Anesthesia Inhibits the Activity of the "Glymphatic System".

    abstract::INTRODUCTION: According to the "glymphatic system" hypothesis, brain waste clearance is mediated by a continuous replacement of the interstitial milieu by a bulk flow of cerebrospinal fluid (CSF). Previous reports suggested that this cerebral CSF circulation is only active during general anesthesia or sleep, an effect...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.19154

    authors: Gakuba C,Gaberel T,Goursaud S,Bourges J,Di Palma C,Quenault A,Martinez de Lizarrondo S,Vivien D,Gauberti M

    更新日期:2018-01-01 00:00:00

  • Coating Solid Lipid Nanoparticles with Hyaluronic Acid Enhances Antitumor Activity against Melanoma Stem-like Cells.

    abstract::Successful anticancer chemotherapy requires targeting tumors efficiently and further potential to eliminate cancer stem cell (CSC) subpopulations. Since CD44 is present on many types of CSCs, and it binds specially to hyaluronic acid (HA), we tested whether coating solid lipid nanoparticles with hyaluronan (HA-SLNs)wo...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.10804

    authors: Shen H,Shi S,Zhang Z,Gong T,Sun X

    更新日期:2015-04-05 00:00:00

  • m6A regulator-based methylation modification patterns characterized by distinct tumor microenvironment immune profiles in colon cancer.

    abstract::Recent studies have highlighted the biological significance of RNA N6-methyladenosine (m6A) modification in tumorigenicity and progression. However, it remains unclear whether m6A modifications also have potential roles in immune regulation and tumor microenvironment (TME) formation. Methods: In this study, we curated...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.52717

    authors: Chong W,Shang L,Liu J,Fang Z,Du F,Wu H,Liu Y,Wang Z,Chen Y,Jia S,Chen L,Li L,Chen H

    更新日期:2021-01-01 00:00:00

  • Modulation of Respiration and Mitochondrial Dynamics by SMAC-Mimetics for Combination Therapy in Chemoresistant Cancer.

    abstract::Inhibitor of apoptosis proteins (IAP) are cell death regulators that bind caspases and interfere with apoptotic signalling via death receptors or intrinsic cell death pathways. BIRC4/XIAP is the most potent anti-apoptotic IAP-member and it physically interacts with caspases via its BIR2 and its BIR3 domain. These doma...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.33758

    authors: Hagenbuchner J,Oberacher H,Arnhard K,Kiechl-Kohlendorfer U,Ausserlechner MJ

    更新日期:2019-07-09 00:00:00

  • RUNX2 overexpression and PTEN haploinsufficiency cooperate to promote CXCR7 expression and cellular trafficking, AKT hyperactivation and prostate tumorigenesis.

    abstract::Rationale: The overall success rate of prostate cancer (PCa) diagnosis and therapy has been improved over the years. However, genomic and phenotypic heterogeneity remains a major challenge for effective detection and treatment of PCa. Efforts to better classify PCa into functional subtypes and elucidate the molecular ...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.33292

    authors: Bai Y,Yang Y,Yan Y,Zhong J,Blee AM,Pan Y,Ma T,Karnes RJ,Jimenez R,Xu W,Huang H

    更新日期:2019-05-24 00:00:00

  • RNA Nanotechnology-Mediated Cancer Immunotherapy.

    abstract::RNA molecules (e.g., siRNA, microRNA, and mRNA) have shown tremendous potential for immunomodulation and cancer immunotherapy. They can activate both innate and adaptive immune system responses by silencing or upregulating immune-relevant genes. In addition, mRNA-based vaccines have recently been actively pursued and ...

    journal_title:Theranostics

    pub_type: 杂志文章,评审

    doi:10.7150/thno.35568

    authors: Lin YX,Wang Y,Blake S,Yu M,Mei L,Wang H,Shi J

    更新日期:2020-01-01 00:00:00

  • Near-Infrared Light Triggered ROS-activated Theranostic Platform based on Ce6-CPT-UCNPs for Simultaneous Fluorescence Imaging and Chemo-Photodynamic Combined Therapy.

    abstract::Many drug controlled release methods have been integrated in multifunctional nanoparticles, such as pH-, redox-, temperature-, enzyme-, and light-responsive release. However, few report is associated with the ROS responsive drug controlled release. Herein, a thioketal linker-based ROS responsive drug (camptothecin con...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.14101

    authors: Yue C,Zhang C,Alfranca G,Yang Y,Jiang X,Yang Y,Pan F,de la Fuente JM,Cui D

    更新日期:2016-02-05 00:00:00

  • Signal amplification and quantification on lateral flow assays by laser excitation of plasmonic nanomaterials.

    abstract::Lateral flow assay (LFA) has become one of the most widely used point-of-care diagnostic methods due to its simplicity and low cost. While easy to use, LFA suffers from its low sensitivity and poor quantification, which largely limits its applications for early disease diagnosis and requires further testing to elimina...

    journal_title:Theranostics

    pub_type: 杂志文章,评审

    doi:10.7150/thno.44298

    authors: Ye H,Liu Y,Zhan L,Liu Y,Qin Z

    更新日期:2020-03-15 00:00:00

  • Endocytosis-mediated mitochondrial transplantation: Transferring normal human astrocytic mitochondria into glioma cells rescues aerobic respiration and enhances radiosensitivity.

    abstract::Emerging evidence indicates that reprogramming of energy metabolism involving disturbances in energy production from a defect in cellular respiration with a shift to glycolysis is a core hallmark of cancer. Alterations in cancer cell energy metabolism are linked to abnormalities in mitochondrial function. Mitochondria...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.33100

    authors: Sun C,Liu X,Wang B,Wang Z,Liu Y,Di C,Si J,Li H,Wu Q,Xu D,Li J,Li G,Wang Y,Wang F,Zhang H

    更新日期:2019-05-26 00:00:00

  • Involvement of reactive oxygen species in sonodynamically induced apoptosis using a novel porphyrin derivative.

    abstract::In this study, we investigated the induction of apoptosis by ultrasound in the presence of the novel porphyrin derivative DCPH-P-Na(I). HL-60 cells were exposed to ultrasound for up to 3 min in the presence and absence of DCPH-P-Na(I), and the induction of apoptosis was examined by analyzing cell morphology, DNA fragm...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.3899

    authors: Yumita N,Iwase Y,Nishi K,Komatsu H,Takeda K,Onodera K,Fukai T,Ikeda T,Umemura S,Okudaira K,Momose Y

    更新日期:2012-01-01 00:00:00

  • Supramolecular Assemblies of Peptides or Nucleopeptides for Gene Delivery.

    abstract::Using non-covalent interactions between nucleic acids (DNA, siRNA, miRNA, and mRNA) with peptides or nucleopeptides is a promising strategy to construct supramolecular assemblies for gene delivery and therapy. Comparing to conventional strategies for gene delivery, the assemblies of peptides or nucleopeptides provide ...

    journal_title:Theranostics

    pub_type: 杂志文章,评审

    doi:10.7150/thno.31854

    authors: Wang H,Feng Z,Xu B

    更新日期:2019-05-18 00:00:00

  • Near-infrared fluorescence imaging in the largely unexplored window of 900-1,000 nm.

    abstract::Near-infrared (NIR) fluorescence imaging has relied on fluorophores that emit in the 700-900 nm NIR-Ia or 1,000-1,700 nm NIR-II window for generating deep-tissue images. Up until now, there have been few fluorophores developed for the 900-1,000 nm NIR-Ib window. This is largely because NIR-Ib light is thought to be st...

    journal_title:Theranostics

    pub_type: 杂志文章

    doi:10.7150/thno.26539

    authors: Deng G,Li S,Sun Z,Li W,Zhou L,Zhang J,Gong P,Cai L

    更新日期:2018-07-16 00:00:00

  • Adaptive changes induced by noble-metal nanostructures in vitro and in vivo.

    abstract::The unique features of noble-metal nanostructures (NMNs) are leading to unprecedented expansion of research and exploration of their application in therapeutics, diagnostics and bioimaging fields. With the ever-growing applications of NMNs, both therapeutic and environmental NMNs are likely to be exposed to tissues an...

    journal_title:Theranostics

    pub_type: 杂志文章,评审

    doi:10.7150/thno.42569

    authors: Huang Q,Zhang J,Zhang Y,Timashev P,Ma X,Liang XJ

    更新日期:2020-04-27 00:00:00