Integrated Analysis of mRNA and miRNA Expression Profiles in the Ovary of Oryctolagus cuniculus in Response to Gonadotrophic Stimulation.


:Molecular mechanisms responsible for gonadotrophic control of ovarian follicle development and ovulation have not been fully delineated. In this study, prepubertal female rabbits were subjected to a combined PMSG/hCG treatment for the induction of follicle maturation and ovulation. Ovaries of 6 does at different time points during gonadotrophic stimulation were collected for histomorphological examination and genome-wide analysis of miRNA and mRNA transcriptomes, and the plasma were separated for detecting melatonin (MT), prostaglandin E2 (PGE2), estradiol (E2), and progesterone (P4) levels. The results suggested that PMSG promoted the development of the reproductive tract by decreasing plasma levels of E2 and slightly increasing those of MT and PGE2 and that hCG induced ovulation and corpus luteum formation by significantly increasing MT, PGE2, and P4 levels. At the transcriptomic level, a total of 1,122 differentially expressed genes (DEGs) and 12 DE miRNAs were identified using three-group comparisons. Meanwhile, pairwise comparisons revealed that 279 and 103 genes as well as 36 and 20 miRNAs were up- and down-regulated during PMSG-stimulated follicle development while 11 and 5 genes as well as 33 and 16 miRNAs were up- and down-regulated during hCG-induced luteinization. KEGG enrichment analysis of the DEGs derived from both three-group- and two-group comparisons as well as the predicted target genes of DE miRNAs highlighted the crucial roles of pathways involving tissue remodeling, energy metabolism, and regulation of cellular functions in mediating gonadotrophin-induced follicle maturation. Specifically, 3 genes including the matrix metallopeptidase 13 (MMP13), protein phosphatase 1 regulatory subunit 3C (PPP1R3C), and solute carrier family 2 member 12 (SLC2A12), together with 2 miRNAs including the miR-205-1 and miR-34c, were predicted to be the promising downstream targets of both PMSG and hCG. Significantly, the miRNA-mRNA interaction pairs containing top 10 up- and down-regulated mRNAs/miRNAs upon PMSG/hCG stimulation were established, and so were those involved in the PI3K-Akt, ECM-receptor interaction, and focal adhesion pathways during PMSG-induced follicle maturation. Finally, qRT-PCR analysis confirmed the results from RNA-Seq and Small RNA-Seq. Our work may contribute to a better understanding of the regulatory mechanisms of gonadotrophins on ovarian follicle development and ovulation.


Hu S,Liang X,Ren X,Shi Y,Su H,Li Y,Du K,Wang J,Jia X,Chen S,Lai S




Has Abstract


2019-10-29 00:00:00








  • Three Therapeutic Strategies: Cinacalcet, Paricalcitol or Both in Secondary Hyperparathyroidism Treatment in Hemodialysed Patients During 1-Year Observational Study-A Comparison.

    abstract::Introduction: Secondary hyperparathyroidism (sHPT) is a common hormonal complication of chronic kidney disease. There are several therapeutic options for sHPT management aiming at calcium-phosphorus balance normalization and decrease of parathormone secretion. Objectives: The aim of this retrospective, observational s...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Zawierucha J,Malyszko J,Malyszko JS,Prystacki T,Marcinkowski WP,Dryl-Rydzynska T

    更新日期:2019-02-05 00:00:00

  • Hippocampal synthesis of sex steroids and corticosteroids: essential for modulation of synaptic plasticity.

    abstract::Sex steroids play essential roles in the modulation of synaptic plasticity and neuroprotection in the hippocampus. Accumulating evidence shows that hippocampal neurons synthesize both estrogen and androgen. Recently, we also revealed the hippocampal synthesis of corticosteroids. The accurate concentrations of these hi...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Hojo Y,Higo S,Kawato S,Hatanaka Y,Ooishi Y,Murakami G,Ishii H,Komatsuzaki Y,Ogiue-Ikeda M,Mukai H,Kimoto T

    更新日期:2011-10-10 00:00:00

  • Enhanced Mortality to Metastatic Bladder Cancer Cell Line MB49 in Vasoactive Intestinal Peptide Gene Knockout Mice.

    abstract::To identify if the absence of the vasoactive intestinal peptide (VIP) gene enhances susceptibility to death from metastatic bladder cancer, two strains of mice were injected with MB49 murine bladder cancer cells. The growth and spread of the cancer was measured over a period of 4 weeks in C57BL/6 mice and 5 weeks in V...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Mirsaidi N,Burns MP,McClain SA,Forsyth E,Li J,Dukes B,Lin D,Nahvi R,Giraldo J,Patton M,Wang P,Lin K,Miller E,Ratliff T,Hamidi S,Crist S,Takemaru KI,Szema A

    更新日期:2017-08-07 00:00:00

  • TSH Receptor Homodimerization in Regulation of cAMP Production in Human Thyrocytes in vitro.

    abstract::Thyrotropin hormone (TSH) was reported to exhibit biphasic regulation of cAMP production in human thyroid slices; specifically, upregulation at low TSH doses transitioning to inhibition at high doses. We observed this phenomenon in HEK293 cells overexpressing TSH receptors (TSHRs) but in only 25% of human thyrocytes (...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Boutin A,Krieger CC,Marcus-Samuels B,Klubo-Gwiezdzinska J,Neumann S,Gershengorn MC

    更新日期:2020-04-30 00:00:00

  • Coexistence of Myelolipoma and Primary Bilateral Macronodular Adrenal Hyperplasia With GIP-Dependent Cushing's Syndrome.

    abstract::Introduction: Adrenal myelolipomas are usually isolated benign adrenal lesions, but can be adjacent to steroid-secreting adrenocortical tumors. We studied the aberrant regulation of cortisol secretion in a 61 year-old woman with combined bilateral myelolipomas and primary bilateral macronodular adrenal hyperplasia (BM...

    journal_title:Frontiers in endocrinology



    authors: Larose S,Bondaz L,Mermejo LM,Latour M,Prosmanne O,Bourdeau I,Lacroix A

    更新日期:2019-09-11 00:00:00

  • Radiotherapy in Fibrodysplasia Ossificans Progressiva: A Case Report and Systematic Review of the Literature.

    abstract::Fibrodysplasia ossificans progressiva (FOP) is an autosomal dominant disease, characterized by the formation of heterotopic ossification (HO) in muscles, ligaments, and tendons. Flare-ups, an inflammatory process that often precedes the formation of HO, can occur spontaneously, but trauma is also a common trigger. It ...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Botman E,Netelenbos JC,Rustemeyer T,Schoonmade LJ,Nieuwenhuijzen JA,Teunissen BP,Visser M,Raijmakers P,Lammertsma AA,Dahele M,Eekhoff M

    更新日期:2020-02-12 00:00:00

  • The Role of Short-Chain Fatty Acids From Gut Microbiota in Gut-Brain Communication.

    abstract::A substantial body of evidence supports that the gut microbiota plays a pivotal role in the regulation of metabolic, endocrine and immune functions. In recent years, there has been growing recognition of the involvement of the gut microbiota in the modulation of multiple neurochemical pathways through the highly inter...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: Silva YP,Bernardi A,Frozza RL

    更新日期:2020-01-31 00:00:00

  • Relational Stability in the Expression of Normality, Variation, and Control of Thyroid Function.

    abstract::Thyroid hormone concentrations only become sufficient to maintain a euthyroid state through appropriate stimulation by pituitary thyroid-stimulating hormone (TSH). In such a dynamic system under constant high pressure, guarding against overstimulation becomes vital. Therefore, several defensive mechanisms protect agai...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: Hoermann R,Midgley JE,Larisch R,Dietrich JW

    更新日期:2016-11-07 00:00:00

  • Progress in the Diagnosis and Classification of Pituitary Adenomas.

    abstract::Pituitary adenomas are common neoplasms. Their classification is based upon size, invasion of adjacent structures, sporadic or familial cases, biochemical activity, clinical manifestations, morphological characteristics, response to treatment and recurrence. Although they are considered benign tumors, some of them are...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: Syro LV,Rotondo F,Ramirez A,Di Ieva A,Sav MA,Restrepo LM,Serna CA,Kovacs K

    更新日期:2015-06-12 00:00:00

  • Kisspeptin and Polycystic Ovary Syndrome.

    abstract::Although the pathogenesis of Polycystic Ovary Syndrome (PCOS) is still unclear, the disturbance of hypothalamic-pituitary-gonadal (HPG) axis is suspected to be the main culprit in the development of PCOS. Kisspeptin, a hypothalamic peptide encoded by the KISS1 gene, is widely reported as a key factor in the regulation...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: Tang R,Ding X,Zhu J

    更新日期:2019-05-10 00:00:00

  • Integrated Multichip Analysis Identifies Potential Key Genes in the Pathogenesis of Nonalcoholic Steatohepatitis.

    abstract:Background:Nonalcoholic steatohepatitis (NASH) is rapidly becoming a major chronic liver disease worldwide. However, little is known concerning the pathogenesis and progression mechanism of NASH. Our aim here is to identify key genes and elucidate their biological function in the progression from hepatic steatosis to N...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Ye J,Lin Y,Wang Q,Li Y,Zhao Y,Chen L,Wu Q,Xu C,Zhou C,Sun Y,Ye W,Bai F,Zhou T

    更新日期:2020-11-26 00:00:00

  • Personality Traits and Physical Complaints in Patients With Acromegaly: A Cross Sectional Multi-Center Study With Analysis of Influencing Factors.

    abstract::Objective: Acromegalic patients display a distinct neuropsychological profile and suffer from chronic physical complaints. We aimed to investigate in more detail these aspects in acromegalic patients, dependent on influencing factors like disease activity, age, sex, chronic medication, surgery, pituitary radiation, pi...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Zimmermann A,Zwerenz R,Droste M,Schöfl C,Strasburger CJ,Plöckinger U,Ziagaki A,Honegger J,Dixius A,Millaku B,Toenges G,Beutel ME,Weber MM

    更新日期:2018-07-17 00:00:00

  • Early Diagnosis of Low-Risk Papillary Thyroid Cancer Results Rather in Overtreatment Than a Better Survival.

    abstract::We are witnessing a rapid worldwide increase in the incidence of papillary thyroid carcinoma (PTC) in the last thirty years. Extensive implementation of cancer screening and wide availability of neck ultrasound or other imaging studies is the main reason responsible for this phenomenon. It resulted in a detection of a...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: Krajewska J,Kukulska A,Oczko-Wojciechowska M,Kotecka-Blicharz A,Drosik-Rutowicz K,Haras-Gil M,Jarzab B,Handkiewicz-Junak D

    更新日期:2020-10-06 00:00:00

  • Environmental and genetic contributors to salivary testosterone levels in infants.

    abstract::Transient activation of the hypothalamic-pituitary-gonadal axis in early infancy plays an important role in male genital development and sexual differentiation of the brain, but factors contributing to individual variation in testosterone levels during this period are poorly understood. We measured salivary testostero...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Xia K,Yu Y,Ahn M,Zhu H,Zou F,Gilmore JH,Knickmeyer RC

    更新日期:2014-10-30 00:00:00

  • Altered Gray Matter Volume in Patients With Type 1 Diabetes Mellitus.

    abstract::Background and Purpose: Many imaging studies have reported structure alterations in patients with type 1 diabetes mellitus (T1DM) by using voxel-based morphometry (VBM). Nevertheless, the results reported were inconsistent and had not been reviewed quantitatively. Accordingly, the quantitative meta-analysis which incl...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Liu J,Fan W,Jia Y,Su X,Wu W,Long X,Sun X,Liu J,Sun W,Zhang T,Gong Q,Shi H,Zhu Q,Wang J

    更新日期:2020-02-13 00:00:00

  • Family History of Diabetes Is Associated With Delayed Fetal Postprandial Brain Activity.

    abstract::Introduction: We have previously shown that fetuses of mothers with gestational diabetes mellitus (GDM) and insulin resistance exhibit a prolongation of fetal auditory event-related brain responses (fAER) compared to fetuses of normal glucose tolerant women during an oral glucose tolerance test (oGTT). This implies th...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Schleger F,Linder K,Walter L,Heni M,Brändle J,Brucker S,Pauluschke-Fröhlich J,Weiss M,Häring HU,Preissl H,Fritsche A

    更新日期:2018-11-20 00:00:00

  • Quantitative analysis of neurotransmitter pathways under steady state conditions - a perspective.

    abstract::In a contribution to this Research Topic Erkki Somersalo and Daniela Calvetti carried out a mathematical analysis of neurotransmitter pathways in brain, modeling compartmental nitrogen flux among several major participants - ammonia, glutamine, glutamate, GABA, and selected amino acids. This analysis is important beca...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: Cooper AJ

    更新日期:2013-11-18 00:00:00

  • Knowledge Gaps and Emerging Research Areas in Intrauterine Growth Restriction-Associated Brain Injury.

    abstract::Intrauterine growth restriction (IUGR) is a complex global healthcare issue. Concerted research and clinical efforts have improved our knowledge of the neurodevelopmental sequelae of IUGR which has raised the profile of this complex problem. Nevertheless, there is still a lack of therapies to prevent the substantial r...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: Fleiss B,Wong F,Brownfoot F,Shearer IK,Baud O,Walker DW,Gressens P,Tolcos M

    更新日期:2019-03-29 00:00:00

  • Clinical Relevance of Genetic Analysis in Patients With Pituitary Adenomas: A Systematic Review.

    abstract::Pituitary adenomas (PA) are amongst the most prevalent intracranial tumors, causing complications by hormonal overproduction or deficiency and tumor mass effects, with 95% of cases occurring sporadically. Associated germline mutations (AIP, MEN1, CDKN1B, PRKAR1A, SDHx) and Xq26.3 microduplications are increasingly ide...

    journal_title:Frontiers in endocrinology



    authors: van den Broek MFM,van Nesselrooij BPM,Verrijn Stuart AA,van Leeuwaarde RS,Valk GD

    更新日期:2019-12-10 00:00:00

  • The Importance of an Early and Accurate MEN1 Diagnosis.

    abstract::Multiple Endocrine Neoplasia type 1 (MEN1) is a rare autosomal dominant inherited condition, causing significant morbidity, and a reduction of life expectancy. A timely and accurate diagnosis of MEN1 is paramount to improve disease outcomes. This enables early identification of tumor manifestations allowing timely tre...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: de Laat JM,van Leeuwaarde RS,Valk GD

    更新日期:2018-09-11 00:00:00

  • Opposite T3 Response of ACTG1-FOS Subnetwork Differentiate Tailfin Fate in Xenopus Tadpole and Post-hatching Axolotl.

    abstract::Amphibian post-embryonic development and Thyroid Hormones (TH) signaling are deeply and intimately connected. In anuran amphibians, TH induce the spectacular and complex process known as metamorphosis. In paedomorphic salamanders, at similar development time, raising levels of TH fail to induce proper metamorphosis, a...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Kerdivel G,Blugeon C,Fund C,Rigolet M,Sachs LM,Buisine N

    更新日期:2019-04-02 00:00:00

  • The IGF/Insulin-IGFBP Axis in Corneal Development, Wound Healing, and Disease.

    abstract::The insulin-like growth factor (IGF) family plays key roles in growth and development. In the cornea, IGF family members have been implicated in proliferation, differentiation, and migration, critical events that maintain a smooth refracting surface that is essential for vision. The IGF family is composed of multiple ...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: Stuard WL,Titone R,Robertson DM

    更新日期:2020-03-03 00:00:00

  • TTF-1 Positive Primary Small Cell Carcinoma of the Breast: A Case Report and Review of the Literature.

    abstract::Primary small cell carcinoma of the breast (SCCB) is a rare tumor subtype comprising <0.1% of all breast carcinomas. Here we present a case of thyroid transcription factor-1 (TTF-1) positive SCCB that recurred within 3 years of diagnosis in the lung and lymph nodes. Given the small number of cases, no clear guidelines...

    journal_title:Frontiers in endocrinology



    authors: Boutrid H,Kassem M,Tozbikian G,Morgan E,White J,Shah M,Vandeusen J,Sardesai S,Williams N,Stover DG,Lustberg M,Wesolowski R,Pudavalli V,Williams TM,Konda B,Fortier S,Carbone D,Ramaswamy B,Cherian MA

    更新日期:2020-04-29 00:00:00

  • Finite Element Analysis-Based Vertebral Bone Strength Prediction Using MDCT Data: How Low Can We Go?

    abstract::Objective: To study the impact of dose reduction in MDCT images through tube current reduction or sparse sampling on the vertebral bone strength prediction using finite element (FE) analysis for fracture risk assessment. Methods: Routine MDCT data covering lumbar vertebrae of 12 subjects (six male; six female; 74.70 ±...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Rayudu NM,Subburaj K,Mei K,Dieckmeyer M,Kirschke JS,Noël PB,Baum T

    更新日期:2020-07-28 00:00:00

  • Cholecystokinin-From Local Gut Hormone to Ubiquitous Messenger.

    abstract::Cholecystokinin (CCK) was discovered in 1928 in jejunal extracts as a gallbladder contraction factor. It was later shown to be member of a peptide family, which are all ligands for the CCK1 and CCK2 receptors. CCK peptides are known to be synthetized in small intestinal endocrine I-cells and cerebral neurons. But in a...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: Rehfeld JF

    更新日期:2017-04-13 00:00:00

  • Increased Requirement of Replacement Doses of Levothyroxine Caused by Liver Cirrhosis.

    abstract:Background:Since hypothyroidism is a fairly common dysfunction, levothyroxine (L-T4) is one of the most prescribed medications. Approximately 70% of the administered L-T4 dose is absorbed. The absorption process takes place in the small intestine. Some disorders of the digestive system and some medicines, supplements, ...

    journal_title:Frontiers in endocrinology



    authors: Benvenga S,Capodicasa G,Perelli S,Ferrari SM,Fallahi P,Antonelli A

    更新日期:2018-04-18 00:00:00

  • Developmental Exposure to 2,2',4,4'-Tetrabromodiphenyl Ether Permanently Alters Blood-Liver Balance of Lipids in Male Mice.

    abstract::Polybrominated diphenyl ethers (PBDEs) were used as flame-retardant additives starting 1965 and were recently withdrawn from commerce in North America and Europe. Approximately 1/5 of the total U.S. population were born when environmental concentrations of PBDE plateaued at their maximum. Accumulating evidence suggest...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Khalil A,Cevik SE,Hung S,Kolla S,Roy MA,Suvorov A

    更新日期:2018-09-20 00:00:00

  • TMT Based Proteomic Analysis of Human Follicular Fluid From Overweight/Obese and Normal-Weight Patients With Polycystic Ovary Syndrome.

    abstract::Background: Polycystic ovary syndrome (PCOS) is a major endocrine and metabolic disorder with heterogeneous manifestations and complex etiology. As a leading cause of anovulatory infertility, the molecular diversity of the follicular microenvironment has not been fully elucidated. The aim of the present study was to i...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Zhang X,Xu X,Li P,Zhou F,Kong L,Qiu J,Yuan Z,Tan J

    更新日期:2019-11-26 00:00:00

  • Metabolomic Assessment Reveals Alteration in Polyols and Branched Chain Amino Acids Associated With Present and Future Renal Impairment in a Discovery Cohort of 637 Persons With Type 1 Diabetes.

    abstract::Background: Improved understanding of the pathophysiology causing diabetic kidney disease (DKD) is imperative. The aim of this study was to uncover associations between serum metabolites and renal outcomes. Methods: Non-targeted serum metabolomics analyses were performed in samples from 637 persons with type 1 diabete...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章


    authors: Tofte N,Suvitaival T,Trost K,Mattila IM,Theilade S,Winther SA,Ahluwalia TS,Frimodt-Møller M,Legido-Quigley C,Rossing P

    更新日期:2019-11-22 00:00:00

  • Experimental Evidence and Clinical Implications of Pituitary Adenoma Stem Cells.

    abstract::Pituitary adenomas, accounting for 15% of diagnosed intracranial neoplasms, are usually benign and pharmacologically and surgically treatable; however, the critical location, mass effects and hormone hypersecretion sustain their significant morbidity. Approximately 35% of pituitary tumors show a less benign course sin...

    journal_title:Frontiers in endocrinology

    pub_type: 杂志文章,评审


    authors: Würth R,Thellung S,Corsaro A,Barbieri F,Florio T

    更新日期:2020-02-20 00:00:00