Conditioned Serum Enhances the Chondrogenic and Immunomodulatory Behavior of Mesenchymal Stem Cells.


:Osteoarthritis is one of the most common chronic health conditions associated with pain and disability. Advanced therapies based on mesenchymal stem cells have become valuable options for the treatment of these pathologies. Conditioned serum (CS, "Orthokine") has been used intra-articularly for osteoarthritic patients. In this work, we hypothesized that the rich content on anti-inflammatory proteins and growth factors of CS may exert a beneficial effect on the biological activity of human adipose-derived mesenchymal stem cells (hAdMSCs). In vitro studies were designed using hAdMSCs cocultured with CS at different concentrations (2.5, 5, and 10%). Chondrogenic differentiation assays and immunomodulatory experiments using in vitro-stimulated lymphocytes were performed. Our results demonstrated that CS significantly enhanced the differentiation of hAdMSCs toward chondrocytes. Moreover, hAdMSCs pre-sensitized with CS reduced the lymphocyte proliferation as well as their differentiation toward activated lymphocytes. These results suggest that in vivo coadministration of CS and hAdMSCs may have a beneficial effect on the therapeutic potential of hAdMSCs. Moreover, these results indicate that intra-articular administration of CS might influence the biological behavior of resident stem cells increasing their chondrogenic differentiation and inherent immunomodulatory activity. To our knowledge, this is the first in vitro study reporting this combination.


Front Pharmacol


Blázquez R,Sánchez-Margallo FM,Reinecke J,Álvarez V,López E,Marinaro F,Casado JG




Has Abstract


2019-06-28 00:00:00








  • Anti-hyperuricemic and Anti-inflammatory Effects of Marantodes pumilum as Potential Treatment for Gout.

    abstract::Marantodes pumilum (Primulaceae) has been used in Malaysian folk medicine to help women regain strength after delivery and for "sickness in the bones." It was previously revealed that its extracts inhibited xanthine oxidase (XO) activity in vitro. The leaves and roots of M. pumilum var. alata (MPA), var. pumila (MPP),...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Rahmi EP,Kumolosasi E,Jalil J,Husain K,Buang F,Abd Razak AF,Jamal JA

    更新日期:2020-03-17 00:00:00

  • The LRRC8A Mediated "Swell Activated" Chloride Conductance Is Dispensable for Vacuolar Homeostasis in Neutrophils.

    abstract::The dialysis of human and mouse neutrophils in patch clamp experiments in the conventional whole-cell mode induces the emergence of a chloride (Cl-) current that appeared to be primarily regulated by cytoplasmic ionic strength. The characteristics of this current resembled that of the classical, and ubiquitous volume-...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Behe P,Foote JR,Levine AP,Platt CD,Chou J,Benavides F,Geha RS,Segal AW

    更新日期:2017-05-12 00:00:00

  • Applying a Global Sensitivity Analysis Workflow to Improve the Computational Efficiencies in Physiologically-Based Pharmacokinetic Modeling.

    abstract::Traditionally, the solution to reduce parameter dimensionality in a physiologically-based pharmacokinetic (PBPK) model is through expert judgment. However, this approach may lead to bias in parameter estimates and model predictions if important parameters are fixed at uncertain or inappropriate values. The purpose of ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Hsieh NH,Reisfeld B,Bois FY,Chiu WA

    更新日期:2018-06-08 00:00:00

  • Chromatin Regulation by HP1γ Contributes to Survival of 5-Azacytidine-Resistant Cells.

    abstract::Recent investigations of the treatment for hematologic neoplasms have focused on targeting epigenetic regulators. The DNA methyltransferase inhibitor 5-azacytidine (AZA) has produced good results in the treatment of patients with myelodysplastic syndromes. The mechanism underlying its pharmacological activity involves...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Imanishi S,Umezu T,Kobayashi C,Ohta T,Ohyashiki K,Ohyashiki JH

    更新日期:2018-10-16 00:00:00

  • Blockade of Serotonin 5-HT2A Receptors Suppresses Behavioral Sensitization and Naloxone-Precipitated Withdrawal Symptoms in Morphine-Treated Mice.

    abstract::The increasing prescription of opioids is fueling an epidemic of addiction and overdose deaths. Morphine is a highly addictive drug characterized by a high relapse rate - even after a long period of abstinence. Serotonin (5-HT) neurotransmission participates in the development of morphine dependence, as well as the ex...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Pang G,Wu X,Tao X,Mao R,Liu X,Zhang YM,Li G,Stackman RW Jr,Dong L,Zhang G

    更新日期:2016-12-26 00:00:00

  • Gleditsia Saponin C Induces A549 Cell Apoptosis via Caspase-Dependent Cascade and Suppresses Tumor Growth on Xenografts Tumor Animal Model.

    abstract::Saponins are natural compounds and possess the most promising anti-cancer function. Here, a saponin gleditsia saponin C (GSC), extracted from gleditsiae fructus abnormalis, could induce apoptosis of lung tumor cell line A549 via caspase dependent cascade and this effect could be prevented by the caspase inhibitors. In...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Cheng Y,He W,He Y

    更新日期:2018-01-15 00:00:00

  • Combined Oral Administration of GABA and DPP-4 Inhibitor Prevents Beta Cell Damage and Promotes Beta Cell Regeneration in Mice.

    abstract::γ-aminobutyric acid (GABA) or glucagon-like peptide-1 based drugs, such as sitagliptin (a dipeptidyl peptidase-4 inhibitor), were shown to induce beta cell regenerative effects in various diabetic mouse models. We propose that their combined administration can bring forth an additive therapeutic effect. We tested this...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Liu W,Son DO,Lau HK,Zhou Y,Prud'homme GJ,Jin T,Wang Q

    更新日期:2017-06-20 00:00:00

  • Type I PRMT Inhibition Protects Against C9ORF72 Arginine-Rich Dipeptide Repeat Toxicity.

    abstract::Repeat expansion mutations in the C9ORF72 gene are the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Repeat-associated non-AUG translation of this expansion produces dipeptide repeat proteins (DRPs). The arginine containing DRPs, polyGR and polyPR, are consistently...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Premasiri AS,Gill AL,Vieira FG

    更新日期:2020-09-08 00:00:00

  • Pathways Connecting Late-Life Depression and Dementia.

    abstract::Late-life depression is associated with significant cognitive impairment. Meta-analyses showed that depression is associated with an increased risk for Alzheimer's disease (AD) and it might be an etiological factor for AD. Since late-life depression is often connected with cognitive impairment and dementia is usually ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Linnemann C,Lang UE

    更新日期:2020-03-13 00:00:00

  • Folliculin Interacts with Rab35 to Regulate EGF-Induced EGFR Degradation.

    abstract::Aims and Hypothesis: This study aims to investigate the mechanism involved in intracellular regulation of EGFR degradation induced by EGF. Methods: Phosphorylation of proteins related to EGFR signaling was examined by western blot analysis. Activation, connection between Rab35 and folliculin (FLCN) were assessed by pu...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Zheng J,Duan B,Sun S,Cui J,Du J,Zhang Y

    更新日期:2017-09-26 00:00:00

  • Quinoxaline 1,4-di-N-Oxides: Biological Activities and Mechanisms of Actions.

    abstract::Quinoxaline 1,4-di-N-oxides (QdNOs) have manifold biological properties, including antimicrobial, antitumoral, antitrypanosomal and antiinflammatory/antioxidant activities. These diverse activities endow them broad applications and prospects in human and veterinary medicines. As QdNOs arouse widespread interest, the e...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Cheng G,Sa W,Cao C,Guo L,Hao H,Liu Z,Wang X,Yuan Z

    更新日期:2016-03-21 00:00:00

  • Protein Kinase D1 (PKD1) Is a New Functional Non-Genomic Target of Bisphenol A in Breast Cancer Cells.

    abstract::Exposure to bisphenol A (BPA), one of the most widespread endocrine disruptors present in our environment, has been associated with the recent increased prevalence and severity of several diseases such as diabetes, obesity, autism, reproductive and neurological defects, oral diseases, and cancers such as breast tumors...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Merzoug-Larabi M,Youssef I,Bui AT,Legay C,Loiodice S,Lognon S,Babajko S,Ricort JM

    更新日期:2020-01-31 00:00:00

  • Supramammillary serotonin reduction alters place learning and concomitant hippocampal, septal, and supramammillar theta activity in a Morris water maze.

    abstract::Hippocampal theta activity is related to spatial information processing, and high-frequency theta activity, in particular, has been linked to efficient spatial memory performance. Theta activity is regulated by the synchronizing ascending system (SAS), which includes mesencephalic and diencephalic relays. The supramam...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Hernández-Pérez JJ,Gutiérrez-Guzmán BE,López-Vázquez MÁ,Olvera-Cortés ME

    更新日期:2015-10-29 00:00:00

  • Terphenyllin Suppresses Orthotopic Pancreatic Tumor Growth and Prevents Metastasis in Mice.

    abstract::Pancreatic cancer (PC) is an aggressive and fatal disease with high incidences of metastasis and recurrence. However, there are no effective treatment options for the majority of PC patients, especially for those with locally advanced tumors and metastatic diseases. Therefore, it is urgently needed to develop safe and...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Zhang J,Wang W,Zhou Y,Yang J,Xu J,Xu Z,Xu B,Yan L,Cheng XD,Li M,Qin JJ

    更新日期:2020-04-08 00:00:00

  • Potential Targeting of Renal Fibrosis in Diabetic Kidney Disease Using MicroRNAs.

    abstract::Diabetic kidney disease (DKD) is a major health problem and one of the leading causes of end-stage renal disease worldwide. Despite recent advances, there exists an urgent need for the development of new treatments for DKD. DKD is characterized by the excessive synthesis and deposition of extracellular matrix proteins...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Sakuma H,Hagiwara S,Kantharidis P,Gohda T,Suzuki Y

    更新日期:2020-11-13 00:00:00

  • P2X4 Receptor in Silico and Electrophysiological Approaches Reveal Insights of Ivermectin and Zinc Allosteric Modulation.

    abstract::Protein allosteric modulation is a pillar of metabolic regulatory mechanisms; this concept has been extended to include ion channel regulation. P2XRs are ligand-gated channels activated by extracellular ATP, sensitive to trace metals and other chemicals. By combining in silico calculations with electrophysiological re...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Latapiat V,Rodríguez FE,Godoy F,Montenegro FA,Barrera NP,Huidobro-Toro JP

    更新日期:2017-12-15 00:00:00

  • Ontological and Non-Ontological Resources for Associating Medical Dictionary for Regulatory Activities Terms to SNOMED Clinical Terms With Semantic Properties.

    abstract::Background: Formal definitions allow selecting terms (e.g., identifying all terms related to "Infectious disease" using the query "has causative agent organism") and terminological reasoning (e.g., "hepatitis B" is a "hepatitis" and is an "infectious disease"). However, the standard international terminology Medical D...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Bousquet C,Souvignet J,Sadou É,Jaulent MC,Declerck G

    更新日期:2019-09-10 00:00:00

  • Rho Kinase Inhibition with Fasudil in the SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis-Symptomatic Treatment Potential after Disease Onset.

    abstract::Despite an improved understanding of the genetic background and the pathomechanisms of amyotrophic lateral sclerosis (ALS) no novel disease-modifying therapies have been successfully implemented in clinical routine. Riluzole still remains the only clinically approved substance in human ALS treatment with limited effic...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Günther R,Balck A,Koch JC,Nientiedt T,Sereda M,Bähr M,Lingor P,Tönges L

    更新日期:2017-01-31 00:00:00

  • Phytoestrogen Bakuchiol Exhibits In Vitro and In Vivo Anti-breast Cancer Effects by Inducing S Phase Arrest and Apoptosis.

    abstract::Phytoestrogen has been proposed as an alternative to hormone replacement therapy, which has been demonstrated to promote a high risk of breast cancer. However, the effect of phytoestrogen on breast cancer development has not been fully understood. Bakuchiol is an active ingredient of a traditional Chinese herbal medic...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Li L,Chen X,Liu CC,Lee LS,Man C,Cheng SH

    更新日期:2016-05-24 00:00:00

  • Toxic Effects of Trichloroethylene on Rat Neuroprogenitor Cells.

    abstract::Trichloroethylene (TCE) is a common volatile organic solvent which is considered as an ubiquitous environmental pollutant. It is claimed to be a developmental neurotoxicant. Our group evaluated previously its impact on three-dimensional neurospheres in vitro. The current work aims to investigate the neurotoxic effects...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Salama MM,El-Naggar DA,Abdel-Rahman RH,Elhak SAG

    更新日期:2018-07-10 00:00:00

  • Small Molecules, Inhibitors of DNA-PK, Targeting DNA Repair, and Beyond.

    abstract::Many current chemotherapies function by damaging genomic DNA in rapidly dividing cells ultimately leading to cell death. This therapeutic approach differentially targets cancer cells that generally display rapid cell division compared to normal tissue cells. However, although these treatments are initially effective i...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Davidson D,Amrein L,Panasci L,Aloyz R

    更新日期:2013-01-31 00:00:00

  • Leaf and Root Extracts from Campomanesia adamantium (Myrtaceae) Promote Apoptotic Death of Leukemic Cells via Activation of Intracellular Calcium and Caspase-3.

    abstract::Phytochemical studies are seeking new alternatives to prevent or treat cancer, including different types of leukemias. Campomanesia adamantium, commonly known as guavira or guabiroba, exhibits pharmacological properties including antioxidant, antimicrobial, and antiproliferative activities. Considering the anticancer ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Campos JF,Espindola PPT,Torquato HFV,Vital WD,Justo GZ,Silva DB,Carollo CA,de Picoli Souza K,Paredes-Gamero EJ,Dos Santos EL

    更新日期:2017-08-14 00:00:00

  • The Pharmacological Potential of Non-ribosomal Peptides from Marine Sponge and Tunicates.

    abstract::Marine biodiversity is recognized by a wide and unique array of fascinating structures. The complex associations of marine microorganisms, especially with sponges, bryozoans, and tunicates, make it extremely difficult to define the biosynthetic source of marine natural products or to deduce their ecological significan...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Agrawal S,Adholeya A,Deshmukh SK

    更新日期:2016-10-25 00:00:00

  • Role of Platelet-Derived Microvesicles As Crosstalk Mediators in Atherothrombosis and Future Pharmacology Targets: A Link between Inflammation, Atherosclerosis, and Thrombosis.

    abstract::Reports in the last decade have suggested that the role of platelets in atherosclerosis and its thrombotic complications may be mediated, in part, by local secretion of platelet-derived microvesicles (pMVs), small cell blebs released during the platelet activation process. MVs are the most abundant cell-derived microv...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Badimon L,Suades R,Fuentes E,Palomo I,Padró T

    更新日期:2016-08-31 00:00:00

  • A Novel Network Pharmacology Strategy to Decode Mechanism of Lang Chuang Wan in Treating Systemic Lupus Erythematosus.

    abstract::Complex disease is a cascade process which is associated with functional abnormalities in multiple proteins and protein-protein interaction (PPI) networks. One drug one target has not been able to perfectly intervene complex diseases. Increasing evidences show that Chinese herb formula usually treats complex diseases ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Gao Y,Wang KX,Wang P,Li X,Chen JJ,Zhou BY,Tian JS,Guan DG,Qin XM,Lu AP

    更新日期:2020-10-02 00:00:00

  • Enzymatic Activities of CYP3A4 Allelic Variants on Quinine 3-Hydroxylation In Vitro.

    abstract::Cytochrome P450 3A4 (CYP3A4) enzyme activity is known to show considerable ethnic heterogeneity and inter-individual differences, affecting the outcome of drug treatment. CYP3A4 genetic polymorphisms are believed to be one of the important causes, leading to inter-individual variability in drug metabolism. Quinine is ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Zhou XY,Hu XX,Wang CC,Lu XR,Chen Z,Liu Q,Hu GX,Cai JP

    更新日期:2019-05-31 00:00:00

  • Targeted Delivery of Curcumin to Polyethylene-Induced Osteolysis by Magnetically Guided Zoledronate-Anchored Poly Lactic-Co-Glycolic Acid Nanoparticles via Repressing NF-κB Signaling.

    abstract::Aseptic loosening induced by periprosthetic osteolysis (PPO) is the leading complication of total joint arthroplasty (TJA) and results in patients having to receive revision surgery. However, there is still no efficient drug to prevent or even slow the pathological process. Herein, we report novel dual-targeted, curcu...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Li J,Niu C,Jiang Z,Zhang Z,Pan Y,Xing Q,Guo Q,An S,Hu Y,Wang L

    更新日期:2020-12-04 00:00:00

  • Pre-clinical Pharmacokinetic and Metabolomic Analyses of Isorhapontigenin, a Dietary Resveratrol Derivative.

    abstract::Background: Isorhapontigenin (trans-3,5,4'-trihydroxy-3'-methoxystilbene, ISO), a dietary resveratrol (trans-3,5,4'-trihydroxystilbene) derivative, possesses various health-promoting activities. To further evaluate its medicinal potentials, the pharmacokinetic and metabolomic profiles of ISO were examined in Sprague-D...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Dai Y,Yeo SCM,Barnes PJ,Donnelly LE,Loo LC,Lin HS

    更新日期:2018-07-11 00:00:00

  • Antithrombotic Regimens in Patients With Percutaneous Coronary Intervention Whom an Anticoagulant Is Indicated: A Systematic Review and Network Meta-Analysis.

    abstract::Background: Patients undergoing percutaneous coronary intervention (PCI) who require anticoagulant therapy are at increased risk of bleeding. The optimal regimen for these patients is uncertain. This study aimed to compare safety and efficacy of antithrombotic regimens used in patients undergoing PCI with concomitant ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Bunmark W,Jinatongthai P,Vathesatogkit P,Thakkinstian A,Reid CM,Wongcharoen W,Chaiyakunapruk N,Nathisuwan S

    更新日期:2018-11-19 00:00:00

  • Mifepristone for Treatment of Metabolic Syndrome: Beyond Cushing's Syndrome.

    abstract::A growing body of research indicates that cortisol, the glucocorticoid product of the activation of the hypothalamic-pituitary-adrenal axis, plays a role in the pathophysiology of metabolic syndrome. In this regard, chronic exposure to cortisol is associated with risk factors related to metabolic syndrome like weight ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Díaz-Castro F,Monsalves-Álvarez M,Rojo LE,Del Campo A,Troncoso R

    更新日期:2020-04-24 00:00:00