Abstract:
:Angiotensin II type I receptor agonistic autoantibodies (AT1-AA) in the plasma of preeclampsia patients can induce apoptosis of cardiomyocytes, and microRNA-21 (miR-21) can exert a protective effect on cardiomyocytes. But whether the pro-apoptotic effect of AT1-AA is associated with miR-21 is unclear. The objective of the present study was to explore whether AT1-AA induced cardiomyocyte apoptosis was related to its inhibitory of miR-21 expression. In vivo studies, the pregnant rats were divided into two groups: Sham group, Model group. The pathology, cell apoptosis, and relative protein expressions were evaluated by hematoxylin and eosin staining, and Western blot assay. The expression of microRNA was detected by gene microarray. In the cell experiment, the neonatal rat cardiomyocytes were divided into four groups: NC group, AT1-AA group, and miR-21 group and AT1-AA+miR-21 group. The cell apoptosis and relative proteins' expressions were measured by flow cytometry and Western blot assay. Results: Compared with the Sham group, miR-21 in the cardiac tissue of the model group was downregulated significantly; the expression of p-JNK, Bax and caspases-3 was increased, the expression of Bcl-2 was decreased, and the Bcl-2/Bax ratio became smaller. The expression of miR-21 in AT1-AA treated cardiomyocytes was only 52% of the control group, with an apoptosis rate of 32.6%. In addition, the expression of pPTEN, pAKT and pFOXO3a in the model group was significantly higher than that in the NC group. The cardiomyocyte apoptosis rate in miR-21 overexpression group was only 23.7%, which was higher than that in the NC group, but significantly lower than that in AT1-AA group. PTEN, AKT and FOXO3a phosporylation in miR-21 overexpression group was also lower than that in AT1-AA group. AT1-AA induced cardiomyocyte apoptosis by downregulating miR-21, and the PTEN/AKT/FOXO3a signal transduction pathway participated in this process. The result of the present study suggests that miR-21 may prove to be a new target for the diagnosis and treatment of preeclampsia and other cardiovascular diseases.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Wang J,Yue J,Xia Q,Jiao X,Zhi Jsubject
Has Abstractpub_date
2019-04-15 00:00:00pages
2339-2349issue
4issn
1943-8141journal_volume
11pub_type
杂志文章abstract::Obesity is a global epidemic that has shown a steady increase in morbimortality indicators; it is considered a social problem and entails serious health risks. One of the alternatives in the treatment of obesity is the traditional use of medicinal plants, which supports the research and development of obesity phytothe...
journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2017-05-15 00:00:00
abstract::Liver cancer is ranked as the 5th major type of cancer and is responsible for significant number of human deaths across the world. Recent investigations have shown microRNAs (miRs) to be involved in diverse cellular processes. Additionally, they have been shown to exhibit therapeutic implications in treatment of diffe...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-04-15 00:00:00
abstract::As a dual-acting neurotransmitter, glycine plays critical roles in cerebral ischemia by activating both glycine receptors (GlyRs) and N-methyl-D-aspartate acid receptors (NMDARs). However, the involvement of glycine receptor alpha 2 (GlyRa2) in cerebral ischemia has not been explored. The objective of this study was t...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-10-15 00:00:00
abstract::The miR-200 family suppresses epithelial-mesenchymal transition by inhibiting ZEB1 and ZEB2 mRNA translation in several types of cancers. Kindlin-2 is a target gene of miR-200b and its expression level correlates positively to ZEB2 in oral squamous cell carcinoma (OSCC). Whether Kindlin-2 and ZEB2 share a competitive ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-08-15 00:00:00
abstract::MicroRNA-302a-5p (miR-302a-5p) has been implicated in several cancers; however, its role in human non-small cell lung carcinoma (NSCLC) remains unknown. In this study, we showed that miR-302a-5p is downregulated in NSCLC tissues and cell lines. Cell Counting Kit-8 and 5-ethynyl-2'-deoxyuridine assays showed that overe...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-07-15 00:00:00
abstract::Bronchopulmonary dysplasia (BPD) is one of the most common causes of mortality and morbidity in neonatal intensive care units. Persistent inflammation, with an abnormal influx of polymorphonuclear leukocytes (PMNs) followed by monocytes (MONOs), occurs early in the pathogenesis of BPD. Anti-inflammatory therapy with b...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Alisertib (MLN8237, ALS), an Aurora kinase A (AURKA) inhibitor, exerts potent anti-tumor effects in the treatment of solid tumor and hematologic malignancies in preclinical and clinical studies. However, the fully spectrum of molecular targets of ALS and its anticancer effect in the treatment of chronic myeloid leukem...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-11-15 00:00:00
abstract::Ulcerative colitis (UC) is a chronic and nonspecific intestinal inflammatory disease, which may increase the risk of colon cancer. Andrographolide is a main active component of Andrographis paniculata. The anti-inflammatory ability of andrographolide suggested its potential therapeutic effect against UC. In the presen...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-02-15 00:00:00
abstract::Understanding the relationships between glomerular endothelial cells (GECs) and glomerular mesangial cells (GMCs) is important to identify the molecular mechanisms underlying diabetic nephropathy (DN). Exosomes carried with mRNA, microRNA, and protein play important roles in cell-to-cell communication. In this study, ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-08-15 00:00:00
abstract::Ankylosing spondylitis (AS) is a type of rheumatic inflammatory disease. miRNAs participate in the process of regulating inflammatory response and bone differentiation. Herein, we aimed to test the effect of miR-495 on AS. The serum and tissues were obtained from traumatic fracture (health) and AS patients. The human ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-05-15 00:00:00
abstract::Growing evidence has revealed that the initiation of various malignancies is closely associated with alternative splicing (AS) events in certain key oncogenes. However, in diffuse large B-cell lymphoma (DLBCL), there is still a great deal to learn about AS variants. In this study, 33,724 AS variant profiles were obtai...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-08-15 00:00:00
abstract::Long non-coding RNAs (lncRNAs) have been recently reported to be dysregulated and play a critical role in the progression of thyroid cancer. Here, we found that the lncRNA n340790 was highly expressed in human thyroid cancer tissues and was strongly correlated with the clinical characteristics of patients. There was a...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-05-15 00:00:00
abstract::In this study, we investigated the biological function of pyruvate kinase M2 (PKM2) and its regulation by deregulated microRNAs (miRNAs) in thyroid cancer (TC). The mRNA and protein expression of PKM2 was examined by quantitative reverse transcription PCR and western blot. The biological role of PKM2 was demonstrated ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-06-15 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Salvianolic acid A (SAA) is a main component derived from Salvia miltiorrhiza and has been revealed to protect against cerebral ischemia/reperfusion injury (CIRI). The present study was designed to evaluate the neuroprotective role of SAA in CIRI and explore its underlying mechanism in vivo an...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-07-15 00:00:00
abstract:BACKGROUND:Gastric cancer (GC) is one of the most common digestive tract tumors, and a serious threat to human health. Long non-coding RNA (lncRNA) are involved in many cancers. However, the biological functions of most lncRNAs are unclear. In this study, we investigated the mechanisms by which FLVCR1-AS1 regulated GC ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-02-15 00:00:00
abstract::Activated protein C (APC) is a vitamin-K dependent natural anticoagulant protein. With its function in blood clotting reaction, APC can reduce the risk of venous thrombosis to prevent ischemic disease. A number of in vivo and in vitro studies over the past few decades have revealed that APC also exerted cytoprotective...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2009-07-15 00:00:00
abstract::Circular RNAs (circRNAs) belong to non-coding RNAs and are known as key regulators in gene regulation. CircRNAs involve in the various biological processes of cancer. However, the functions of circRNAs in renal cell carcinoma (RCC) are still not clear. In this study, the circRNA expression profile was performed in the...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-08-15 00:00:00
abstract::RAB18, a member of the Ras family, has been suggested to play a critical role in multiple biological process. However, its functions in the development of hepatocellular carcinoma (HCC) remain unknown. In the present study, the expression and biological role of RAB18 in HCC were investigated. Results showed that the e...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-02-15 00:00:00
abstract::Glioma-associated oncogene homolog 1 (GLI1), an oncogenic molecule in non-small cell lung cancer (NSCLC), promotes the growth of NSCLC by enhancing lung cancer stem cells (LCSCs). However, the mechanism responsible remains unknown. FOXP3 is known to maintain LCSCs. The aim of this study was to explore whether GLI1 enh...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-05-15 00:00:00
abstract::HNF1A-antisense 1 (HNF1A-AS1), a long non-coding RNA (lncRNA), is associated with metastasis and is an independent prognostic factor for lung cancer. Recent studies demonstrated that HNF1A-AS1 play important roles in cacinogenesis. However, the exact effects and molecular mechanisms of HNF1A-AS1 in osteosarcoma (OS) p...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-08-15 00:00:00
abstract::Formula-3 is a Chinese herbal medicine formula that was shown to inhibit food allergy in rats by stabilizing mast cells. But whether Formula-3 ameliorates food allergy through modulating the composition of intestinal microbiota remains to be explored. Here, we aimed to determine whether gut microbiota mediate the anti...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-09-15 00:00:00
abstract::Progression of abdominal aortic aneurysm (AAA) is typified by chronic inflammation and extracellular matrix (ECM) degradation of the aortic wall. Vascular inflammation involves complex interactions among inflammatory cells, endothelial cells (ECs), vascular smooth muscle cells (vSMCs), and ECM. Although vascular endot...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-12-15 00:00:00
abstract::Nonalcoholic steatohepatitis (NASH) has similar clinical pathological changes to alcoholic hepatitis. It shows increased incidence and young trend year by year. Polyene phosphatidyl choline (PPC) is widely used in clinic for liver disease treatment. The effect and mechanism of PPC on NASH have not been fully elucidate...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-05-15 00:00:00
abstract::The quantity and function of osteoblasts require continuous osteogenic differentiation of bone marrow mesenchymal stem cells. Recent evidence suggests that microRNAs (miRNAs) act as important post-transcriptional regulators in a wide range of biological processes, including osteoblastic differentiation. Quercetin has ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-05-15 00:00:00
abstract::Angiotesin II (Ang II) plays an important role in cardiac remodeling. Fibroblast growth factor inducible-14 (Fn14) is the smallest member of the tumor necrosis factor superfamily of receptors. Currently, little is known about the functional role of Fn14 in the heart. Chiefly, we observe the up-regulation of extracellu...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-12-15 00:00:00
abstract:BACKGROUND:Recurrent hepatocellular carcinoma (rHCC) patients with microvascular invasive (MVI) positive at first resection usually had poorly differentiated tumors and worse survivals. The optimal treatment for this population remains to be elucidated. METHODS:We retrospectively analyzed 319 rHCC patients with MVI-po...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-03-15 00:00:00
abstract::The aim of the present research was to investigate the association between the BRAF mutation and papillary thyroid carcinoma (PTC), and further explore the relationship between the systemic inflammation response index (SIRI) and BRAF mutation in patients with PTC. The clinicopathological data were extracted from the p...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-08-15 00:00:00
abstract::Myocardial ischemia/reperfusion (I/R) injury is a common cardiovascular disease with high morbidity and mortality globally, which derives from acute myocardial infarction and coronary artery disease. Elabela has been proved to bind to apelin receptors in the heart. The present study aimed to investigate the protective...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-08-15 00:00:00
abstract::Pyrroloquinoline quinone (PQQ), considered as an ROS scavenger,could protect mitochondrial activity from damage of oxidative stress. To determine the role of PQQ supplement in rescuing long bone osteoporosis in Bmi-1-/- mice. We fed Bmi-1 knockout mice a diet supplemented with PQQ (BKO+PQQ), BKO mice with normal diet ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-10-15 00:00:00
abstract:OBJECTIVE:To create the rabbit animal model of intervertebral disc (IVD) degeneration by the annulus needle puncture technique through a novel transabdominal approach. METHODS:Thirteen New Zealand White rabbits underwent annular puncture at the L3/4, L4/5, and L5/6 discs through a transabdominal approach. For a longit...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-03-15 00:00:00