Abstract:
:BACKGROUND Sesquiterpene lactones have gained tremendous attention owing to their potent anticancer properties. The main focus of this study was to evaluate the anticancer effects of a naturally occurring sesquiterpene lactone, santonin, against human breast cancer SK-BR-3 cells. MATERIAL AND METHODS Cell counting kit 8 assay was used for the determination of cell viability. Apoptosis was detected by DAPI (4',6-diamidino-2-phenylindole) and annexin V/propidium iodide (IP) staining. Flow cytometry was used for cell cycle analysis and western blotting was used for the estimation of protein expression. RESULTS Results showed that santonin exerts significant anti-proliferative effects on the SK-BR-3 breast cancer cells in a concentration dependent manner. Santonin showed an IC₅₀ of 16 µM against SK-BR-3 cells. DAPI staining showed that santonin caused DNA fragmentation in the SK-BR-3 cells, which is indicative of apoptosis. Annexin V/PI staining showed that apoptotic cell percentage increased up to 34.32% at 32 µM concentration of santonin. Santonin also caused an increase in the expression of Bax, caspase-3, and caspase-9, and a decrease in the expression of Bcl-2. Santonin also caused the arrest of the SK-BR-3 cells at the G2/M phase of the cell cycle and suppressed the expression of cyclin A and B1. Finally, santonin could also block the Raf/MEK/ERK pathway in breast cancer cells. CONCLUSIONS The findings of this study suggest the potential for the naturally occurring sesquiterpene lactone santonin in breast cancer treatment and also suggest that it could be developed as a promising anticancer agent.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Wu Z,Wang C,Huang M,Tao Z,Yan W,Du Ydoi
10.12659/MSM.915246subject
Has Abstractpub_date
2019-05-18 00:00:00pages
3676-3682eissn
1234-1010issn
1643-3750pii
915246journal_volume
25pub_type
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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