Abstract：
:BACKGROUND The aim of this study was to investigate the role of deubiquitinase [ovarian tumor domain-containing protein 5 (OTUD5)] in regulating Akt ubiquitination and its effect on the radiosensitivity of cervical cancer. MATERIAL AND METHODS Cervical cancer C33A cells were cultured, and then 2 groups of cells (overexpressed cells and silenced cells) were established by overexpressing and silencing OTUD5 gene. Next, quantitative polymerase chain reaction (qPCR) was employed to detect the expression level of OTUD5 in cells in each group. Co-immunoprecipitation and Western blot (WB) analysis were applied to measure the expression level of phosphorylated protein kinase B (Akt) and the level of ubiquitination. The sensitivity of cells to radiotherapy in each group was detected via clone-forming efficiency assay. After that, Statistical Product and Service Solutions (SPSS) 17.0 software was employed for analyses. The t test, one-way analysis of variance (ANOVA), and p test were used. P<0.05 suggested that a difference was statistically significant. RESULTS The levels of phosphorylated Akt and ubiquitination in OTUD5-overexpressed C33A cells were lower than those in the OTUD5-silenced group and control group. The sensitivity of OTUD5-overexpressed C33A cells to radiotherapy was higher than that of the OTUD5-silenced group and control group. CONCLUSIONS OTUD5 affects the radiosensitivity of cervical cancer through the regulation of Akt deubiquitination.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Yin F,He H,Zhang B,Zheng J,Wang M,Zhang M,Cui Hdoi
10.12659/MSM.912904subject
Has Abstractpub_date
2019-05-10 00:00:00pages
3469-3475eissn
1234-1010issn
1643-3750pii
912904journal_volume
25pub_type
杂志文章abstract：:BACKGROUND Post-traumatic syringomyelia (PTS) is a common disease after spinal cord injury (SCI). The present study was performed to evaluate the advantages of diffusion tensor imaging (DTI) in estimating SCI and prognosing PTS in SCI rats. MATERIAL AND METHODS Forty rats were divided into 3 groups based on the extent...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.907955
更新日期：2018-01-09 00:00:00
abstract：BACKGROUND:Aortic cross-clamping during abdominal aortic aneurysm (AAA) open repair leads to development of ischemia-reperfusion injury. Electron paramagnetic resonance spectroscopy (EPR) spin-trapping is a valuable method of direct measurement of free radicals. The objective of the study was to evaluate the results of...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.890774
更新日期：2014-11-27 00:00:00
abstract：BACKGROUND:Hormonal studies in patients with multiple sclerosis are rare and they often produce results which are difficult to interpret. These investigations, however, are becoming more and more important as they may cast some light on possible interrelationships between hormonal and immune systems. The aim of the pre...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：BACKGROUND:The purpose of this study was to investigate the values of contrast-enhanced ultrasound (CEUS) in the diagnosis and differential diagnosis of hyperechoic liver lesions. MATERIAL AND METHODS:The CEUS findings of 102 patients with hyperechoic liver lesions identified by 2-dimensional ultrasound in the Affilia...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.894115
更新日期：2015-09-22 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.890124
更新日期：2014-08-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2008-04-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.910932
更新日期：2018-12-06 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.917097
更新日期：2019-09-22 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.914564
更新日期：2019-03-13 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,收录出版
doi：10.12659/MSM.907816
更新日期：2018-07-26 00:00:00
abstract：BACKGROUND:We investigated the clinical value of serum levels of neuron-specific enolase (NSE) and human soluble protein-100β (S-100β) in acute cerebral infarction (ACI) patients. MATERIAL AND METHODS:A literature search of electronic databases identified relevant case-control studies that examined the correlations be...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,meta分析
doi：10.12659/msm.893615
更新日期：2015-06-30 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.909162
更新日期：2018-07-19 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.895689
更新日期：2016-06-20 00:00:00
abstract：BACKGROUND:To evaluate the per-operative process of shoulderjoint replacement, time-action analysis can be used. MATERIAL/METHODS:Forty procedures performed by 7 surgeons with different experience using 5 different prostheses and 3 different surgical approaches were analyzed. RESULTS:The surgical procedures showed a ...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:BACKGROUND Doxorubicin (DOX) is a potent chemotherapeutic agent used to treat colon cancer. Despite impressive initial clinical responses, drug resistance has dramatically compromised the effectiveness of DOX. However, the underlying mechanisms of chemotherapeutic resistance in colon cancer remain poorly understood. M...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.913325
更新日期：2019-03-24 00:00:00
abstract：BACKGROUND:In the last decade there has been increasing interest in cytoskeletal research in relation to important process of cell biology. However, after decades of debate many issues remain unsettled. The aim of our study was to estimate the distribution of tubulin in etoposide-induced HL-60 cells and address the que...
journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.889443
更新日期：2013-09-09 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2003-02-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章,评审
doi：
更新日期：2003-10-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.893307
更新日期：2015-04-28 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2007-08-01 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.893514
更新日期：2015-07-31 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.920793
更新日期：2020-03-23 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.907146
更新日期：2018-07-09 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.924783
更新日期：2020-08-15 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/MSM.893467
更新日期：2015-06-24 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.905127
更新日期：2018-02-28 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：10.12659/msm.905393
更新日期：2018-01-23 00:00:00
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journal_title：Medical science monitor : international medical journal of experimental and clinical research
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
