Determination of the Maximum Velocity of Filaments in the in vitro Motility Assay.

Abstract:

:The in vitro motility assay (IVMA) is a powerful tool commonly used in basic muscle research and for drug screenings with the aim to find treatment options for neuromuscular disorders. In brief, the sliding movement of fluorescence-labeled actin filaments on myosin motor proteins is recorded, and the sliding velocity is analyzed via image analysis methods. Due to low signal-to-noise ratios and large variability in the velocity signal, accurate determination of the maximum sliding velocity is challenging. We introduce a new method and software program named Actin Phase Velocity (ActiPHV). The method extracts the maximum velocity from filament tracking data. Based on simulated and real reference data we show that our method yields a higher accuracy compared to previous methods. As a result, our method enables enhancing the sensitivity of the IVMA to better exploit its full potential.

journal_name

Front Physiol

journal_title

Frontiers in physiology

authors

Bopp N,Scheid LM,Fink RHA,Rohr K

doi

10.3389/fphys.2019.00289

subject

Has Abstract

pub_date

2019-03-27 00:00:00

pages

289

issn

1664-042X

journal_volume

10

pub_type

杂志文章
  • Dental Findings in Patients With Non-surgical Hypoparathyroidism and Pseudohypoparathyroidism: A Systematic Review.

    abstract::Background: Dental aberrations have been mentioned in relation to non-surgical hypoparathyroidism (Ns-HypoPT) and pseudohypoparathyroidism (PHP). However, a systematic review of dental characteristics have not been performed. The present systematic review describes the dental findings in patients with Ns-HypoPT and PH...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.00701

    authors: Hejlesen J,Underbjerg L,Gjørup H,Bloch-Zupan A,Sikjaer T,Rejnmark L,Haubek D

    更新日期:2018-06-19 00:00:00

  • High-Intensity Interval Training in Normobaric Hypoxia Leads to Greater Body Fat Loss in Overweight/Obese Women than High-Intensity Interval Training in Normoxia.

    abstract::A moderate hypoxic stimulus is considered a promising therapeutic modality for several pathological states including obesity. There is scientific evidence suggesting that when hypoxia and physical activity are combined, they could provide benefits for the obese population. The aim of the present study was to investiga...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.00060

    authors: Camacho-Cardenosa A,Camacho-Cardenosa M,Burtscher M,Martínez-Guardado I,Timon R,Brazo-Sayavera J,Olcina G

    更新日期:2018-02-07 00:00:00

  • Comprehensive Uncertainty Quantification and Sensitivity Analysis for Cardiac Action Potential Models.

    abstract::Recent efforts to ensure the reliability of computational model-based predictions in healthcare, such as the ASME V&V40 Standard, emphasize the importance of uncertainty quantification (UQ) and sensitivity analysis (SA) when evaluating computational models. UQ involves empirically determining the uncertainty in model ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.00721

    authors: Pathmanathan P,Cordeiro JM,Gray RA

    更新日期:2019-06-26 00:00:00

  • The Origin of a Trans-Generational Organization in the Phenomenon of Biogenesis.

    abstract::One of the central issues of the whole process of biogenesis is how to understand the progressive constitution of a large (in spatial and temporal terms) system that transcends the individual sphere of proto-metabolic organizations and includes collective networks, both synchronous (i.e., proto-ecosystem webs) and asy...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2019.01222

    authors: Moreno A

    更新日期:2019-09-24 00:00:00

  • Effect of Low Versus High Tidal-Volume Total Liquid Ventilation on Pulmonary Inflammation.

    abstract::Animal experiments suggest that total liquid ventilation (TLV) induces less ventilator-induced lung injury (VILI) than conventional mechanical gas ventilation. However, TLV parameters that optimally minimize VILI in newborns remain unknown. Our objective was to compare lung inflammation between low (L-VT) and high (H-...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00603

    authors: Sage M,See W,Nault S,Morin C,Michalski C,Chabot B,Marouan S,Lavoie PM,Micheau P,Praud JP,Fortin-Pellerin É

    更新日期:2020-06-18 00:00:00

  • Solenoidal Micromagnetic Stimulation Enables Activation of Axons With Specific Orientation.

    abstract::Electrical stimulation of the central and peripheral nervous systems - such as deep brain stimulation, spinal cord stimulation, and epidural cortical stimulation are common therapeutic options increasingly used to treat a large variety of neurological and psychiatric conditions. Despite their remarkable success, there...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.00724

    authors: Golestanirad L,Gale JT,Manzoor NF,Park HJ,Glait L,Haer F,Kaltenbach JA,Bonmassar G

    更新日期:2018-07-27 00:00:00

  • Angiotensin II Type 1 Receptors and Systemic Hemodynamic and Renal Responses to Stress and Altered Blood Volume in Conscious Rabbits.

    abstract::We examined how systemic blockade of type 1 angiotensin (AT(1)-) receptors affects reflex control of the circulation and the kidney. In conscious rabbits, the effects of candesartan on responses of systemic and renal hemodynamics and renal excretory function to acute hypoxia, mild hemorrhage, and plasma volume expansi...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2011.00040

    authors: Xu TB,Eppel GA,Head GA,Evans RG

    更新日期:2011-07-19 00:00:00

  • Pathophysiological Mechanisms of Sino-Atrial Dysfunction and Ventricular Conduction Disease Associated with SCN5A Deficiency: Insights from Mouse Models.

    abstract::Genetically modified mice provide a number of models for studying cardiac channelopathies related to cardiac Na(+) channel (SCN5A) abnormalities. We review key pathophysiological features in these murine models that may underlie clinical features observed in sinus node dysfunction and progressive cardiac conduction di...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2012.00234

    authors: Huang CL,Lei L,Matthews GD,Zhang Y,Lei M

    更新日期:2012-07-06 00:00:00

  • Glial Cells Are Involved in ANG-II-Induced Vasopressin Release and Sodium Intake in Awake Rats.

    abstract::It is known that circulating angiotensin II (ANG-II) acts on the circumventricular organs (CVOs), which partially lack a normal blood-brain barrier, to stimulate pressor responses, vasopressin (AVP), and oxytocin (OT) secretion, as well as sodium and water intake. Although ANG-II type 1 receptors (AT1R) are expressed ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.00430

    authors: Flôr AFL,de Brito Alves JL,França-Silva MS,Balarini CM,Elias LLK,Ruginsk SG,Antunes-Rodrigues J,Braga VA,Cruz JC

    更新日期:2018-05-01 00:00:00

  • Hypercaloric Diet Establishes Erectile Dysfunction in Rat: Mechanisms Underlying the Endothelial Damage.

    abstract::Obesity is characterized by an excessive increase in body mass, leading to endothelial damage that may favor the development of erectile dysfunction (ED). ED is defined as the inability to achieve or maintain a penile erection long enough to have a sexual intercourse. In this context, different ED models were develope...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2017.00760

    authors: de Souza ILL,Barros BC,de Oliveira GA,Queiroga FR,Toscano LT,Silva AS,Silva PM,Interaminense LFL,Cavalcante FA,da Silva BA

    更新日期:2017-10-04 00:00:00

  • Muscle cell derived angiopoietin-1 contributes to both myogenesis and angiogenesis in the ischemic environment.

    abstract::Recent strategies to treat peripheral arterial disease (PAD) have focused on stem cell based therapies, which are believed to result in local secretion of vascular growth factors. Little is known, however, about the role of ischemic endogenous cells in this context. We hypothesized that ischemic muscle cells (MC) are ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2015.00161

    authors: McClung JM,Reinardy JL,Mueller SB,McCord TJ,Kontos CD,Brown DA,Hussain SN,Schmidt CA,Ryan TE,Green TD

    更新日期:2015-05-19 00:00:00

  • SR-BI Mediated Transcytosis of HDL in Brain Microvascular Endothelial Cells Is Independent of Caveolin, Clathrin, and PDZK1.

    abstract::The vascular endothelium supplying the brain exhibits very low paracellular and transcellular permeability and is a major constituent of the blood-brain barrier. High-density lipoprotein (HDL) crosses the blood-brain barrier by transcytosis, but technical limitations have made it difficult to elucidate its regulation....

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2017.00841

    authors: Fung KY,Wang C,Nyegaard S,Heit B,Fairn GD,Lee WL

    更新日期:2017-10-30 00:00:00

  • Maximal Oxygen Uptake Is Achieved in Hypoxia but Not Normoxia during an Exhaustive Severe Intensity Run.

    abstract::Highly aerobically trained individuals are unable to achieve maximal oxygen uptake ([Formula: see text]) during exhaustive running lasting ~2 min, instead [Formula: see text] plateaus below [Formula: see text] after ~1 min. Hypoxia offers the opportunity to study the ([Formula: see text]) response to an exhaustive run...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2017.00096

    authors: Black MI,Potter CR,Corbett J,Clark CC,Draper SB

    更新日期:2017-02-21 00:00:00

  • PM2.5, Fine Particulate Matter: A Novel Player in the Epithelial-Mesenchymal Transition?

    abstract::Epithelial-mesenchymal transition (EMT) refers to the conversion of epithelial cells to mesenchymal phenotype, which endows the epithelial cells with enhanced migration, invasion, and extracellular matrix production abilities. These characteristics link EMT with the pathogenesis of organ fibrosis and cancer progressio...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2019.01404

    authors: Xu Z,Ding W,Deng X

    更新日期:2019-11-29 00:00:00

  • The in vivo dsRNA Cleavage Has Sequence Preference in Insects.

    abstract::Exogenous dsRNA enters the insect body and can induce the RNAi effect only when it is cleaved into siRNA. However, what kinds of base composition are easier to cut and what kinds of siRNA will be produced in vivo is largely unknown. In this study, we found that dsRNA processing into siRNA has sequence preference and r...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.01768

    authors: Guan R,Hu S,Li H,Shi Z,Miao X

    更新日期:2018-12-10 00:00:00

  • Individually Rate Corrected QTc Intervals in Children and Adolescents.

    abstract::Accurate evaluation of the appearance of QTc sex differences during childhood and adolescence is intricate. Inter-subject differences of individual QT/RR patterns make generic heart rate corrections inaccurate because of fast resting heart rates in children. The study investigated 527 healthy children and adolescents ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2019.00994

    authors: Andršová I,Hnatkova K,Helánová K,Šišáková M,Novotný T,Kala P,Malik M

    更新日期:2019-08-02 00:00:00

  • On the Role of the Head Ganglia in Posture and Walking in Insects.

    abstract::In insects, locomotion is the result of rhythm generating thoracic circuits and their modulation by sensory reflexes and by inputs from the two head ganglia, the cerebral and the gnathal ganglia (GNG), which act as higher order neuronal centers playing different functions in the initiation, goal-direction, and mainten...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2020.00135

    authors: Emanuel S,Kaiser M,Pflueger HJ,Libersat F

    更新日期:2020-02-21 00:00:00

  • Insects With Survival Kits for Desiccation Tolerance Under Extreme Water Deficits.

    abstract::The year 2002 marked the tercentenary of Antonie van Leeuwenhoek's discovery of desiccation tolerance in animals. This remarkable phenomenon to sustain 'life' in the absence of water can be revived upon return of hydrating conditions. Today, coping with climate change-related factors, especially temperature-humidity i...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2018.01843

    authors: Thorat L,Nath BB

    更新日期:2018-12-21 00:00:00

  • Vulnerability of the developing brain to hypoxic-ischemic damage: contribution of the cerebral vasculature to injury and repair?

    abstract::As clinicians attempt to understand the underlying reasons for the vulnerability of different regions of the developing brain to injury, it is apparent that little is known as to how hypoxia-ischemia may affect the cerebrovasculature in the developing infant. Most of the research investigating the pathogenesis of peri...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2012.00424

    authors: Baburamani AA,Ek CJ,Walker DW,Castillo-Melendez M

    更新日期:2012-11-09 00:00:00

  • Effects of Whole-Body Electromyostimulation on Strength-, Sprint-, and Jump Performance in Moderately Trained Young Adults: A Mini-Meta-Analysis of Five Homogenous RCTs of Our Work Group.

    abstract::Background: Whole-body electromyostimulation (WB-EMS) gained increasing interest in sports within recent years. However, few intervention studies have examined the effects of WB-EMS on trained subjects in comparison to conventional strength training. Objective: The aim of the present mini-meta-analysis of 5 recently c...

    journal_title:Frontiers in physiology

    pub_type:

    doi:10.3389/fphys.2019.01336

    authors: Wirtz N,Dörmann U,Micke F,Filipovic A,Kleinöder H,Donath L

    更新日期:2019-11-08 00:00:00

  • N-cadherin and integrin blockade inhibit arteriolar myogenic reactivity but not pressure-induced increases in intracellular Ca.

    abstract::The vascular myogenic response is characterized by arterial constriction in response to an increase in intraluminal pressure and dilatation to a decrease in pressure. This mechanism is important for the regulation of blood flow, capillary pressure, and arterial pressure. The identity of the mechanosensory mechanism(s)...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2010.00165

    authors: Jackson TY,Sun Z,Martinez-Lemus LA,Hill MA,Meininger GA

    更新日期:2010-12-29 00:00:00

  • Atrial Rotor Dynamics Under Complex Fractional Order Diffusion.

    abstract::The mechanisms of atrial fibrillation (AF) are a challenging research topic. The rotor hypothesis states that the AF is sustained by a reentrant wave that propagates around an unexcited core. Cardiac tissue heterogeneities, both structural and cellular, play an important role during fibrillatory dynamics, so that the ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.00975

    authors: Ugarte JP,Tobón C,Lopes AM,Machado JAT

    更新日期:2018-07-24 00:00:00

  • Quantifying Glioblastoma Drug Response Dynamics Incorporating Treatment Sensitivity and Blood Brain Barrier Penetrance From Experimental Data.

    abstract::Many drugs investigated for the treatment of glioblastoma (GBM) have had disappointing clinical trial results. Efficacy of these agents is dependent on adequate delivery to sensitive tumor cell populations, which is limited by the blood-brain barrier (BBB). Additionally, tumor heterogeneity can lead to subpopulations ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00830

    authors: Massey SC,Urcuyo JC,Marin BM,Sarkaria JN,Swanson KR

    更新日期:2020-08-21 00:00:00

  • Effects of Hypoxia on Erythrocyte Membrane Properties-Implications for Intravascular Hemolysis and Purinergic Control of Blood Flow.

    abstract::Intravascular hemolysis occurs in hereditary, acquired, and iatrogenic hemolytic conditions but it could be also a normal physiological process contributing to intercellular signaling. New evidence suggests that intravascular hemolysis and the associated release of adenosine triphosphate (ATP) may be an important mech...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2017.01110

    authors: Grygorczyk R,Orlov SN

    更新日期:2017-12-22 00:00:00

  • Adipokinetic Hormone Receptor Mediates Trehalose Homeostasis to Promote Vitellogenin Uptake by Oocytes in Nilaparvata lugens.

    abstract::Adipokinetic hormones (AKHs) are well known to mobilize lipids and carbohydrates for energy-consuming activities in insects. These neuropeptides exert their functions by interacting with AKH receptors (AKHRs) located on the plasma membrane of fat body cells, which regulates energy mobilization by stimulating lipolysis...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.01904

    authors: Lu K,Wang Y,Chen X,Zhang X,Li W,Cheng Y,Li Y,Zhou J,You K,Song Y,Zhou Q,Zeng R

    更新日期:2019-01-08 00:00:00

  • Extracellular hemoglobin: the case of a friend turned foe.

    abstract::Hemoglobin (Hb) is a highly conserved molecule present in all life forms and functionally tied to the complexity of aerobic organisms on earth in utilizing oxygen from the atmosphere and delivering to cells and tissues. This primary function sustains the energy requirements of cells and maintains cellular homeostasis....

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审

    doi:10.3389/fphys.2015.00096

    authors: Quaye IK

    更新日期:2015-04-20 00:00:00

  • Enhanced Myogenic Constriction in the SHR Preglomerular Vessels Is Mediated by Thromboxane A2 Synthesis.

    abstract:Background:Spontaneously Hypertensive Rats (SHR) have chronically elevated blood pressures at 30 weeks of age (systolic: 191.0 ± 1.0, diastolic: 128.8 ± 0.9). However, despite this chronic malignant hypertension, SHR kidneys remain relatively free of pathology due to having an augmented myogenic constriction (MC). We h...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00853

    authors: Nademi S,Lu C,Dickhout JG

    更新日期:2020-07-22 00:00:00

  • Metabolic Reprogramming of Mouse Bone Marrow Derived Macrophages Following Erythrophagocytosis.

    abstract::Reticuloendothelial macrophages engulf ∼0.2 trillion senescent erythrocytes daily in a process called erythrophagocytosis (EP). This critical mechanism preserves systemic heme-iron homeostasis by regulating red blood cell (RBC) catabolism and iron recycling. Although extensive work has demonstrated the various effects...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2020.00396

    authors: Catala A,Youssef LA,Reisz JA,Dzieciatkowska M,Powers NE,Marchetti C,Karafin M,Zimring JC,Hudson KE,Hansen KC,Spitalnik SL,D'Alessandro A

    更新日期:2020-04-30 00:00:00

  • The Use of Berlin Heart EXCOR VAD in Children Less than 10 kg: A Single Center Experience.

    abstract::Objective: Despite the improvement in ventricular assist device (VAD) therapy in adults and in adolescents, in infant population only Berlin Heart EXCOR (BHE) is licensed as long term VAD to bridge children to Heart Transplantation (HTx). Particularly demanding in terms of morbidity and mortality are smallest patients...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2016.00614

    authors: Di Molfetta A,Gandolfo F,Filippelli S,Perri G,Di Chiara L,Iacobelli R,Adorisio R,Favia I,Rizza A,Testa G,Di Nardo M,Amodeo A

    更新日期:2016-12-06 00:00:00

  • High Altitude Affects Nocturnal Non-linear Heart Rate Variability: PATCH-HA Study.

    abstract::Background: High altitude (HA) exposure can lead to changes in resting heart rate variability (HRV), which may be linked to acute mountain sickness (AMS) development. Compared with traditional HRV measures, non-linear HRV appears to offer incremental and prognostic data, yet its utility and relationship to AMS have be...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章

    doi:10.3389/fphys.2018.00390

    authors: Boos CJ,Bye K,Sevier L,Bakker-Dyos J,Woods DR,Sullivan M,Quinlan T,Mellor A

    更新日期:2018-04-16 00:00:00