Abstract:
:The in vitro motility assay (IVMA) is a powerful tool commonly used in basic muscle research and for drug screenings with the aim to find treatment options for neuromuscular disorders. In brief, the sliding movement of fluorescence-labeled actin filaments on myosin motor proteins is recorded, and the sliding velocity is analyzed via image analysis methods. Due to low signal-to-noise ratios and large variability in the velocity signal, accurate determination of the maximum sliding velocity is challenging. We introduce a new method and software program named Actin Phase Velocity (ActiPHV). The method extracts the maximum velocity from filament tracking data. Based on simulated and real reference data we show that our method yields a higher accuracy compared to previous methods. As a result, our method enables enhancing the sensitivity of the IVMA to better exploit its full potential.
journal_name
Front Physioljournal_title
Frontiers in physiologyauthors
Bopp N,Scheid LM,Fink RHA,Rohr Kdoi
10.3389/fphys.2019.00289subject
Has Abstractpub_date
2019-03-27 00:00:00pages
289issn
1664-042Xjournal_volume
10pub_type
杂志文章abstract::Background: Dental aberrations have been mentioned in relation to non-surgical hypoparathyroidism (Ns-HypoPT) and pseudohypoparathyroidism (PHP). However, a systematic review of dental characteristics have not been performed. The present systematic review describes the dental findings in patients with Ns-HypoPT and PH...
journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2018.00701
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abstract::A moderate hypoxic stimulus is considered a promising therapeutic modality for several pathological states including obesity. There is scientific evidence suggesting that when hypoxia and physical activity are combined, they could provide benefits for the obese population. The aim of the present study was to investiga...
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abstract::Recent efforts to ensure the reliability of computational model-based predictions in healthcare, such as the ASME V&V40 Standard, emphasize the importance of uncertainty quantification (UQ) and sensitivity analysis (SA) when evaluating computational models. UQ involves empirically determining the uncertainty in model ...
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pub_type: 杂志文章,评审
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abstract::Animal experiments suggest that total liquid ventilation (TLV) induces less ventilator-induced lung injury (VILI) than conventional mechanical gas ventilation. However, TLV parameters that optimally minimize VILI in newborns remain unknown. Our objective was to compare lung inflammation between low (L-VT) and high (H-...
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abstract::Electrical stimulation of the central and peripheral nervous systems - such as deep brain stimulation, spinal cord stimulation, and epidural cortical stimulation are common therapeutic options increasingly used to treat a large variety of neurological and psychiatric conditions. Despite their remarkable success, there...
journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2018.00724
更新日期:2018-07-27 00:00:00
abstract::We examined how systemic blockade of type 1 angiotensin (AT(1)-) receptors affects reflex control of the circulation and the kidney. In conscious rabbits, the effects of candesartan on responses of systemic and renal hemodynamics and renal excretory function to acute hypoxia, mild hemorrhage, and plasma volume expansi...
journal_title:Frontiers in physiology
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doi:10.3389/fphys.2011.00040
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abstract::Genetically modified mice provide a number of models for studying cardiac channelopathies related to cardiac Na(+) channel (SCN5A) abnormalities. We review key pathophysiological features in these murine models that may underlie clinical features observed in sinus node dysfunction and progressive cardiac conduction di...
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doi:10.3389/fphys.2012.00234
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abstract::It is known that circulating angiotensin II (ANG-II) acts on the circumventricular organs (CVOs), which partially lack a normal blood-brain barrier, to stimulate pressor responses, vasopressin (AVP), and oxytocin (OT) secretion, as well as sodium and water intake. Although ANG-II type 1 receptors (AT1R) are expressed ...
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pub_type: 杂志文章
doi:10.3389/fphys.2018.00430
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abstract::Obesity is characterized by an excessive increase in body mass, leading to endothelial damage that may favor the development of erectile dysfunction (ED). ED is defined as the inability to achieve or maintain a penile erection long enough to have a sexual intercourse. In this context, different ED models were develope...
journal_title:Frontiers in physiology
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doi:10.3389/fphys.2017.00760
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journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2015.00161
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abstract::Highly aerobically trained individuals are unable to achieve maximal oxygen uptake ([Formula: see text]) during exhaustive running lasting ~2 min, instead [Formula: see text] plateaus below [Formula: see text] after ~1 min. Hypoxia offers the opportunity to study the ([Formula: see text]) response to an exhaustive run...
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abstract::Exogenous dsRNA enters the insect body and can induce the RNAi effect only when it is cleaved into siRNA. However, what kinds of base composition are easier to cut and what kinds of siRNA will be produced in vivo is largely unknown. In this study, we found that dsRNA processing into siRNA has sequence preference and r...
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doi:10.3389/fphys.2018.01768
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abstract::Accurate evaluation of the appearance of QTc sex differences during childhood and adolescence is intricate. Inter-subject differences of individual QT/RR patterns make generic heart rate corrections inaccurate because of fast resting heart rates in children. The study investigated 527 healthy children and adolescents ...
journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2019.00994
更新日期:2019-08-02 00:00:00
abstract::In insects, locomotion is the result of rhythm generating thoracic circuits and their modulation by sensory reflexes and by inputs from the two head ganglia, the cerebral and the gnathal ganglia (GNG), which act as higher order neuronal centers playing different functions in the initiation, goal-direction, and mainten...
journal_title:Frontiers in physiology
pub_type: 杂志文章,评审
doi:10.3389/fphys.2020.00135
更新日期:2020-02-21 00:00:00
abstract::The year 2002 marked the tercentenary of Antonie van Leeuwenhoek's discovery of desiccation tolerance in animals. This remarkable phenomenon to sustain 'life' in the absence of water can be revived upon return of hydrating conditions. Today, coping with climate change-related factors, especially temperature-humidity i...
journal_title:Frontiers in physiology
pub_type: 杂志文章,评审
doi:10.3389/fphys.2018.01843
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abstract::As clinicians attempt to understand the underlying reasons for the vulnerability of different regions of the developing brain to injury, it is apparent that little is known as to how hypoxia-ischemia may affect the cerebrovasculature in the developing infant. Most of the research investigating the pathogenesis of peri...
journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2012.00424
更新日期:2012-11-09 00:00:00
abstract::Background: Whole-body electromyostimulation (WB-EMS) gained increasing interest in sports within recent years. However, few intervention studies have examined the effects of WB-EMS on trained subjects in comparison to conventional strength training. Objective: The aim of the present mini-meta-analysis of 5 recently c...
journal_title:Frontiers in physiology
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doi:10.3389/fphys.2019.01336
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abstract::The vascular myogenic response is characterized by arterial constriction in response to an increase in intraluminal pressure and dilatation to a decrease in pressure. This mechanism is important for the regulation of blood flow, capillary pressure, and arterial pressure. The identity of the mechanosensory mechanism(s)...
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journal_title:Frontiers in physiology
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doi:10.3389/fphys.2018.00975
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journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2020.00830
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journal_title:Frontiers in physiology
pub_type: 杂志文章,评审
doi:10.3389/fphys.2017.01110
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journal_title:Frontiers in physiology
pub_type: 杂志文章
doi:10.3389/fphys.2018.01904
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pub_type: 杂志文章
doi:10.3389/fphys.2020.00853
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journal_title:Frontiers in physiology
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doi:10.3389/fphys.2020.00396
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doi:10.3389/fphys.2016.00614
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pub_type: 杂志文章
doi:10.3389/fphys.2018.00390
更新日期:2018-04-16 00:00:00