MiR-409-3p Inhibits Cell Proliferation and Invasion of Osteosarcoma by Targeting Zinc-Finger E-Box-Binding Homeobox-1.


:Osteosarcoma (OS) is the most common bone cancer worldwide. There is evidence that microRNA-409 (miR-409-3p) is involved in tumorigenesis and cancer progression, however, its possible role in OS requires clarification. In the present study, we evaluated the expression level, clinical significance, and mode of action of miR-409-3p in OS. The miR-409-3p levels were diminished in the OS cells and tissues compared with associated adjacent non-tumor tissues and a non-cancer osteoplastic cell line. Low miR-409-3p expression levels were associated with clinical stage and distant metastasis in patients with OS. Resumption of miR-409-3p expression attenuated OS cell proliferation and invasion. Additionally, based on informatics analyses, we predicted that zinc-finger E-box-binding homeobox-1 (ZEB1) is a possible target of miR-409-3p. This hypothesis was confirmed using luciferase reporter assays, reverse transcription-quantitative real-time polymerase chain reaction, and Western blot analyses. The findings of the current study indicated that ZEB1 was up-regulated in the OS tissues and cell lines, and that this up-regulation was inversely proportional to miR-409-3p expression levels. Furthermore, down-regulation of ZEB1 decreased OS cell invasion and proliferation, illustrating that the tumor suppressive role of miR-409-3p in OS cells may be exerted via negative regulation of ZEB1. Taken together, our observations highlight the potential role of miR-409-3p as a tumor suppressor in OS partially through down-regulation of ZEB1 and suggest that miR-409-3p has potential applications in OS treatment.


Front Pharmacol


Wu L,Zhang Y,Huang Z,Gu H,Zhou K,Yin X,Xu J




Has Abstract


2019-02-21 00:00:00








  • Erratum on: Epoxyeicosatrienoic acid analog attenuates angiotensin II hypertension and kidney injury.

    abstract::[This corrects the article on p. 216 in vol. 5, PMID: 25295006.]. ...

    journal_title:Frontiers in pharmacology

    pub_type: 已发布勘误


    authors: Frontiers Production Office.

    更新日期:2014-11-19 00:00:00

  • Inhibition of Soluble Epoxide Hydrolase Limits Mitochondrial Damage and Preserves Function Following Ischemic Injury.

    abstract:AIMS:Myocardial ischemia can result in marked mitochondrial damage leading to cardiac dysfunction, as such identifying novel mechanisms to limit mitochondrial injury is important. This study investigated the hypothesis that inhibiting soluble epoxide hydrolase (sEH), responsible for converting epoxyeicosatrienoic acids...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Akhnokh MK,Yang FH,Samokhvalov V,Jamieson KL,Cho WJ,Wagg C,Takawale A,Wang X,Lopaschuk GD,Hammock BD,Kassiri Z,Seubert JM

    更新日期:2016-06-07 00:00:00

  • Clonal evolution and therapeutic resistance in solid tumors.

    abstract::Tumors frequently arise as a result of an acquired genomic instability and the subsequent evolution of neoplastic populations with variable genomes. A barrier to the study of the somatic genetics of human solid tumors in vivo is the presence of admixtures of non-neoplastic cells with normal genomes in patient samples....

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Barrett MT,Lenkiewicz E,Evers L,Holley T,Ruiz C,Bubendorf L,Sekulic A,Ramanathan RK,Von Hoff DD

    更新日期:2013-01-28 00:00:00

  • Long-Chain Omega-3 Fatty Acids Supplementation Accelerates Nerve Regeneration and Prevents Neuropathic Pain Behavior in Mice.

    abstract::Fish oil (FO) is the main source of long chain omega-3 polyunsaturated fatty acids (ω-3 PUFAs), which display relevant analgesic and anti-inflammatory properties. Peripheral nerve injury is driven by degeneration, neuroinflammation, and neuronal plasticity which results in neuropathic pain (NP) symptoms such as allody...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Silva RV,Oliveira JT,Santos BLR,Dias FC,Martinez AMB,Lima CKF,Miranda ALP

    更新日期:2017-10-17 00:00:00

  • Hydrogen Sulfide Inhibits Cigarette Smoke-Induced Endoplasmic Reticulum Stress and Apoptosis in Bronchial Epithelial Cells.

    abstract::Background: Apoptosis of lung structural cells contributes to the process of lung damage and remodeling in chronic obstructive pulmonary disease (COPD). Our previous studies demonstrated that exogenous hydrogen sulfide (H2S) can reduce the lung tissue pathology score, anti-inflammation and anti-oxidation effects in CO...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Lin F,Liao C,Sun Y,Zhang J,Lu W,Bai Y,Liao Y,Li M,Ni X,Hou Y,Qi Y,Chen Y

    更新日期:2017-09-28 00:00:00

  • Pathways Connecting Late-Life Depression and Dementia.

    abstract::Late-life depression is associated with significant cognitive impairment. Meta-analyses showed that depression is associated with an increased risk for Alzheimer's disease (AD) and it might be an etiological factor for AD. Since late-life depression is often connected with cognitive impairment and dementia is usually ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Linnemann C,Lang UE

    更新日期:2020-03-13 00:00:00

  • Salvinorin A Inhibits Airway Hyperreactivity Induced by Ovalbumin Sensitization.

    abstract::Salvinorin A, a neoclerodane diterpene isolated from Salvia divinorum, exerts a number of pharmacological actions which are not solely limited to the central nervous system. Recently it has been demonstrated that Salvinorin A inhibits acute inflammatory response affecting leukotriene (LT) production. Since LTs are pot...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Rossi A,Caiazzo E,Bilancia R,Riemma MA,Pagano E,Cicala C,Ialenti A,Zjawiony JK,Izzo AA,Capasso R,Roviezzo F

    更新日期:2017-01-13 00:00:00

  • Managing the complexity of communication: regulation of gap junctions by post-translational modification.

    abstract::Gap junctions are comprised of connexins that form cell-to-cell channels which couple neighboring cells to accommodate the exchange of information. The need for communication does, however, change over time and therefore must be tightly controlled. Although the regulation of connexin protein expression by transcriptio...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Axelsen LN,Calloe K,Holstein-Rathlou NH,Nielsen MS

    更新日期:2013-10-22 00:00:00

  • Advances and Challenges of Liposome Assisted Drug Delivery.

    abstract::The application of liposomes to assist drug delivery has already had a major impact on many biomedical areas. They have been shown to be beneficial for stabilizing therapeutic compounds, overcoming obstacles to cellular and tissue uptake, and improving biodistribution of compounds to target sites in vivo. This enables...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Sercombe L,Veerati T,Moheimani F,Wu SY,Sood AK,Hua S

    更新日期:2015-12-01 00:00:00

  • Innovative Strategies to Develop Chemical Categories Using a Combination of Structural and Toxicological Properties.

    abstract::Interest is increasing in the development of non-animal methods for toxicological evaluations. These methods are however, particularly challenging for complex toxicological endpoints such as repeated dose toxicity. European Legislation, e.g., the European Union's Cosmetic Directive and REACH, demands the use of altern...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Batke M,Gütlein M,Partosch F,Gundert-Remy U,Helma C,Kramer S,Maunz A,Seeland M,Bitsch A

    更新日期:2016-09-21 00:00:00

  • Anti-Methicillin-Resistant Staphylococcus aureus Nanoantibiotics.

    abstract::Nanoparticle-based antibiotic constructs have become a popular area of investigation in the biomedical sciences. Much of this work has pertained to human diseases, largely in the cancer therapy arena. However, considerable research has also been devoted to the nanochemistry for controlling infectious diseases. Among t...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Labruère R,Sona AJ,Turos E

    更新日期:2019-10-04 00:00:00

  • Serum Concentrations of Cholinesterase Inhibitors in Patients With Alzheimer's Dementia Are Frequently Below the Recommended Levels.

    abstract:Background:Acetylcholinesterase inhibitors (AChE-I) are recommended for the treatment of cognitive symptoms but also of behavioral and psychological symptoms in dementia. They are widely used not only in Alzheimer's disease, but also in other forms of dementia. Efficacy of treatment might depend on serum concentration ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Ortner M,Stange M,Schneider H,Schroeder C,Buerger K,Müller C,Dorn B,Goldhardt O,Diehl-Schmid J,Förstl H,Steimer W,Grimmer T

    更新日期:2020-05-21 00:00:00

  • Exosomes As Potential Biomarkers and Targeted Therapy in Colorectal Cancer: A Mini-Review.

    abstract::The number of colorectal cancer (CRC) cases have increased gradually year by year. In fact, CRC is one of the most widely diagnosed cancer in men and women today. This disease is usually diagnosed at a later stage of the development, and by then, the chance of survival has declined significantly. Even though substanti...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Hon KW,Abu N,Ab Mutalib NS,Jamal R

    更新日期:2017-08-28 00:00:00

  • Aspergillus terreus (Trichocomaceae): A Natural, Eco-Friendly Mycoinsecticide for Control of Malaria, Filariasis, Dengue Vectors and Its Toxicity Assessment Against an Aquatic Model Organism Artemia nauplii.

    abstract::Vector-borne diseases like malaria, filariasis, and dengue are transmitted by mosquitoes and they cause global mortality and morbidity due to an increased resistance against commercial insecticides. The present study was aimed to evaluate the neurobehavioral toxicity, knock-down effect, histopathology, ovicidal, adult...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Ragavendran C,Srinivasan R,Kim M,Natarajan D

    更新日期:2018-11-26 00:00:00

  • Aflatoxin B1 Degradation and Detoxification by Escherichia coli CG1061 Isolated From Chicken Cecum.

    abstract::Aflatoxin B1 (AFB1) is one of the most hazardous mycotoxins contamination in food and feed products, which leads to hepatocellular carcinoma in humans and animals. In the present study, we isolated and characterized an AFB1 degrading bacteria CG1061 from chicken cecum, exhibited an 93.7% AFB1 degradation rate by HPLC....

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Wang L,Wu J,Liu Z,Shi Y,Liu J,Xu X,Hao S,Mu P,Deng F,Deng Y

    更新日期:2019-01-17 00:00:00

  • Validation and Psychometric Properties of the Arabic Version of the Duke Anticoagulation Satisfaction Scale (DASS).

    abstract::Background: To assess the health-related quality of life (HRQoL) of oral anticoagulant therapy users, different types of instruments are available, either general or specific tools like Duke Anticoagulation Satisfaction Scale (DASS). These tools allow the clinician to adjust the treatment regimen to focus on increasin...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: AlAmmari M,Sultana K,AlHarbi SN,Marenga AS,AlTuraiki A,Althemery AU,Alfaifi AA,AlShehri A,Aqeel BF

    更新日期:2020-12-17 00:00:00

  • Transcriptome Profiling and Cytological Assessments for Identifying Regulatory Pathways Associated With Diorcinol N-Induced Autophagy in A3 Cells.

    abstract::Fungal secondary metabolites serve as a rich resource for exploring lead compounds with medicinal importance. Diorcinol N (DN), a fungal secondary metabolite isolated from an endophytic fungus, Arthrinium arundinis, exhibits robust anticancer activity. However, the anticancer mechanism of DN remains unclear. In this s...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Yuan XL,Li XQ,Xu K,Hou XD,Zhang ZF,Xue L,Liu XM,Zhang P

    更新日期:2020-10-15 00:00:00

  • Impact of Biologic Treatment of Crohn's Disease on the Rate of Surgeries and Other Healthcare Resources: An Analysis of a Nationwide Database From Poland.

    abstract::Background: There is conflicting evidence on the impact of biologic treatment on the rate of complications and surgeries in Crohn's disease (CD). We aimed to assess real-world consequences of biologic treatment of CD. Methods: All adult patients with CD treated with infliximab and adalimumab in the years 2012-2014 wer...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Holko P,Kawalec P,Pilc A

    更新日期:2018-06-11 00:00:00

  • Metabolomics Reveals Effect of Zishen Jiangtang Pill, a Chinese Herbal Product on High-Fat Diet-Induced Type 2 Diabetes Mellitus in Mice.

    abstract::A Chinese herbal decoction, Zishen Jiangtang Pill (ZJP), has been clinically prescribed to diabetic patients to prevent excessive blood sugar levels for decades. However, the potential mechanisms of this action have not been well investigated. The purpose of this study was to explore the metabolic variations in respon...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Chen J,Zheng L,Hu Z,Wang F,Huang S,Li Z,Zheng P,Zhang S,Yi T,Li H

    更新日期:2019-03-19 00:00:00

  • A Significant Association Between Rhein and Diabetic Nephropathy in Animals: A Systematic Review and Meta-Analysis.

    abstract::Background: Rhein is considered to have beneficial influence on diabetic nephropathy. Animal experiments suggested that the mechanisms of rhein against diabetic nephropathy may involve many processes, but the credibility of the evidence is unclear. Therefore, we conducted systematic review and meta-analysis of pre-cli...

    journal_title:Frontiers in pharmacology



    authors: Hu HC,Zheng LT,Yin HY,Tao Y,Luo XQ,Wei KS,Yin LP

    更新日期:2019-12-13 00:00:00

  • Identification of Pharmacokinetic Markers for Guanxin Danshen Drop Pills in Rats by Combination of Pharmacokinetics, Systems Pharmacology, and Pharmacodynamic Assays.

    abstract::This paper reported a feasibility study strategy of identifying pharmacokinetic (PK) markers for a cardiovascular herbal medicine, Guanxin Danshen drop pill (GDDP). First, quantification analysis revealed the constituent composition in the preparation by high-performance liquid chromatography (HPLC). Subsequently, phy...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Yao H,Huang X,Xie Y,Huang X,Ruan Y,Lin X,Huang L,Shi P

    更新日期:2018-12-21 00:00:00

  • Recent Initiatives in the Republic of Srpska to Enhance Appropriate Use of Antibiotics in Ambulatory Care; Their Influence and Implications.

    abstract::Introduction: There are increasing concerns world-wide with growing rates of antibiotic resistance necessitating urgent action. There have been a number of initiatives in the Republic of Srpska in recent years to address this and improve rational antibiotic prescribing and dispensing despite limited resources to fund ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Bojanić L,Marković-Peković V,Škrbić R,Stojaković N,Ðermanović M,Bojanić J,Fürst J,Kurdi AB,Godman B

    更新日期:2018-05-29 00:00:00

  • CaMKII Requirement for in Vivo Insular Cortex LTP Maintenance and CTA Memory Persistence.

    abstract::Calcium-calmodulin/dependent protein kinase II (CaMKII) plays an essential role in LTP induction, but since it has the capacity to remain persistently activated even after the decay of external stimuli it has been proposed that it can also be necessary for LTP maintenance and therefore for memory persistence. It has b...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Juárez-Muñoz Y,Ramos-Languren LE,Escobar ML

    更新日期:2017-11-14 00:00:00

  • Environmental Stressors on Skin Aging. Mechanistic Insights.

    abstract::The skin is the main barrier that protects us against environmental stressors (physical, chemical, and biological). These stressors, combined with internal factors, are responsible for cutaneous aging. Furthermore, they negatively affect the skin and increase the risk of cutaneous diseases, particularly skin cancer. T...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Parrado C,Mercado-Saenz S,Perez-Davo A,Gilaberte Y,Gonzalez S,Juarranz A

    更新日期:2019-07-09 00:00:00

  • Rejuveinix Shows a Favorable Clinical Safety Profile in Human Subjects and Exhibits Potent Preclinical Protective Activity in the Lipopolysaccharide-Galactosamine Mouse Model of Acute Respiratory Distress Syndrome and Multi-Organ Failure.

    abstract::Background: New treatment platforms that can prevent acute respiratory distress syndrome (ARDS) or reduce its mortality rate in high-risk coronavirus disease 2019 (COVID-19) patients, such as those with an underlying cancer, are urgently needed. Rejuveinix (RJX) is an intravenous formulation of anti-oxidants and anti-...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Uckun FM,Carlson J,Orhan C,Powell J,Pizzimenti NM,van Wyk H,Ozercan IH,Volk M,Sahin K

    更新日期:2020-11-10 00:00:00

  • The Selective SIK2 Inhibitor ARN-3236 Produces Strong Antidepressant-Like Efficacy in Mice via the Hippocampal CRTC1-CREB-BDNF Pathway.

    abstract::Depression is a widespread chronic medical illness affecting thoughts, mood, and physical health. However, the limited and delayed therapeutic efficacy of monoaminergic drugs has led to intensive research efforts to develop novel antidepressants. ARN-3236 is the first potent and selective inhibitor of salt-inducible k...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Liu Y,Tang W,Ji C,Gu J,Chen Y,Huang J,Zhao X,Sun Y,Wang C,Guan W,Liu J,Jiang B

    更新日期:2021-01-14 00:00:00

  • Icariin, but Not Genistein, Exerts Osteogenic and Anti-apoptotic Effects in Osteoblastic Cells by Selective Activation of Non-genomic ERα Signaling.

    abstract::Genistein and icariin are flavonoid compounds that exhibit estrogen-like properties in inducing bone formation and reducing bone loss associated with estrogen deficiency in both preclinical and clinical studies. However, the mechanisms that are involved in mediating their estrogenic actions in bone cells are far from ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Ho MX,Poon CC,Wong KC,Qiu ZC,Wong MS

    更新日期:2018-05-11 00:00:00

  • Long-Distance Phasing of a Tentative "Enhancer" Single-Nucleotide Polymorphism With CYP2D6 Star Allele Definitions.

    abstract:Background:The CYP2D6 gene locus has been extensively studied over decades, yet a portion of variability in CYP2D6 activity cannot be explained by known sequence variations within the gene, copy number variation, or structural rearrangements. It was proposed that rs5758550, located 116 kb downstream of the CYP2D6 gene ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Boone EC,Wang WY,Gaedigk R,Cherner M,Bérard A,Leeder JS,Miller NA,Gaedigk A

    更新日期:2020-05-08 00:00:00

  • Identification of a Novel EF-Loop in the N-terminus of TRPM2 Channel Involved in Calcium Sensitivity.

    abstract::As an oxidative stress sensor, transient receptor potential melastatin 2 (TRPM2) channel is involved in many physiological and pathological processes including warmth sensing, ischemia injury, inflammatory diseases and diabetes. Intracellular calcium is critical for TRPM2 channel activation and the IQ-like motif in th...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章


    authors: Luo Y,Yu X,Ma C,Luo J,Yang W

    更新日期:2018-06-04 00:00:00

  • Chymase Inhibitor as a Novel Therapeutic Agent for Non-alcoholic Steatohepatitis.

    abstract::Non-alcoholic steatohepatitis (NASH) is characterized by inflammation and fibrosis, in addition to steatosis, of the liver, but no therapeutic agents have yet been established. The mast cell protease chymase can generate angiotensin II, matrix metalloproteinase-9 and transforming growth factor-β, all of which are asso...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审


    authors: Takai S,Jin D

    更新日期:2018-02-21 00:00:00