Evaluation of a novel West Nile virus transmission control strategy that targets Culex tarsalis with endectocide-containing blood meals.


:Control of arbovirus transmission remains focused on vector control through application of insecticides directly to the environment. However, these insecticide applications are often reactive interventions that can be poorly-targeted, inadequate for localized control during outbreaks, and opposed due to environmental and toxicity concerns. In this study, we developed endectocide-treated feed as a systemic endectocide for birds to target blood feeding Culex tarsalis, the primary West Nile virus (WNV) bridge vector in the western United States, and conducted preliminary tests on the effects of deploying this feed in the field. In lab tests, ivermectin (IVM) was the most effective endectocide tested against Cx. tarsalis and WNV-infection did not influence mosquito mortality from IVM. Chickens and wild Eurasian collared doves exhibited no signs of toxicity when fed solely on bird feed treated with concentrations up to 200 mg IVM/kg of diet, and significantly more Cx. tarsalis that blood fed on these birds died (greater than 80% mortality) compared to controls (less than 25% mortality). Mosquito mortality following blood feeding correlated with IVM serum concentrations at the time of blood feeding, which dropped rapidly after the withdrawal of treated feed. Preliminary field testing over one WNV season in Fort Collins, Colorado demonstrated that nearly all birds captured around treated bird feeders had detectable levels of IVM in their blood. However, entomological data showed that WNV transmission was non-significantly reduced around treated bird feeders. With further development, deployment of ivermectin-treated bird feed might be an effective, localized WNV transmission control tool.


PLoS Negl Trop Dis


Nguyen C,Gray M,Burton TA,Foy SL,Foster JR,Gendernalik AL,Rückert C,Alout H,Young MC,Boze B,Ebel GD,Clapsaddle B,Foy BD




Has Abstract


2019-03-07 00:00:00














  • Diagnosis of Persistent Fever in the Tropics: Set of Standard Operating Procedures Used in the NIDIAG Febrile Syndrome Study.

    abstract::In resource-limited settings, the scarcity of skilled personnel and adequate laboratory facilities makes the differential diagnosis of fevers complex [1-5]. Febrile illnesses are diagnosed clinically in most rural centers, and both Rapid Diagnostic Tests (RDTs) and clinical algorithms can be valuable aids to health wo...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Alirol E,Horie NS,Barbé B,Lejon V,Verdonck K,Gillet P,Jacobs J,Büscher P,Kanal B,Bhattarai NR,El Safi S,Phe T,Lim K,Leng L,Lutumba P,Mukendi D,Bottieau E,Boelaert M,Rijal S,Chappuis F

    更新日期:2016-11-03 00:00:00

  • Sero-Molecular Epidemiology of Japanese Encephalitis in Zhejiang, an Eastern Province of China.

    abstract:BACKGROUND:Sporadic Japanese encephalitis (JE) cases still have been reported in Zhejiang Province in recent years, and concerns about vaccine cross-protection and population-level immunity have been raised off and on within the public health sphere. Genotype I (GI) has replaced GIII as the dominant genotype in Asian c...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Pan JR,Yan JY,Zhou JY,Tang XW,He HQ,Xie RH,Mao HY,Zhang YJ,Xie SY

    更新日期:2016-08-25 00:00:00

  • A third generation vaccine for human visceral leishmaniasis and post kala azar dermal leishmaniasis: First-in-human trial of ChAd63-KH.

    abstract:BACKGROUND:Visceral leishmaniasis (VL or kala azar) is the most serious form of human leishmaniasis, responsible for over 20,000 deaths annually, and post kala azar dermal leishmaniasis (PKDL) is a stigmatizing skin condition that often occurs in patients after successful treatment for VL. Lack of effective or appropri...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Osman M,Mistry A,Keding A,Gabe R,Cook E,Forrester S,Wiggins R,Di Marco S,Colloca S,Siani L,Cortese R,Smith DF,Aebischer T,Kaye PM,Lacey CJ

    更新日期:2017-05-12 00:00:00

  • Antibodies against immature virions are not a discriminating factor for dengue disease severity.

    abstract::Humoral immunity plays an important role in controlling dengue virus (DENV) infection. Antibodies (Abs) developed during primary infection protect against subsequent infection with the same dengue serotype, but can enhance disease following secondary infection with a heterologous serotype. A DENV virion has two surfac...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Rodenhuis-Zybert IA,da Silva Voorham JM,Torres S,van de Pol D,Smit JM

    更新日期:2015-03-11 00:00:00

  • A Comparison between Transcriptome Sequencing and 16S Metagenomics for Detection of Bacterial Pathogens in Wildlife.

    abstract:BACKGROUND:Rodents are major reservoirs of pathogens responsible for numerous zoonotic diseases in humans and livestock. Assessing their microbial diversity at both the individual and population level is crucial for monitoring endemic infections and revealing microbial association patterns within reservoirs. Recently, ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Razzauti M,Galan M,Bernard M,Maman S,Klopp C,Charbonnel N,Vayssier-Taussat M,Eloit M,Cosson JF

    更新日期:2015-08-18 00:00:00

  • Safety and efficacy of single dose versus multiple doses of AmBisome for treatment of visceral leishmaniasis in eastern Africa: a randomised trial.

    abstract:BACKGROUND:Anti-leishmanial drug regimens that include a single dose AmBisome could be suitable for eastern African patients with symptomatic visceral leishmaniasis (VL) but the appropriate single dose is unknown. METHODOLOGY:A multi-centre, open-label, non-inferiority, randomized controlled trial with an adaptive des...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Khalil EA,Weldegebreal T,Younis BM,Omollo R,Musa AM,Hailu W,Abuzaid AA,Dorlo TP,Hurissa Z,Yifru S,Haleke W,Smith PG,Ellis S,Balasegaram M,EL-Hassan AM,Schoone GJ,Wasunna M,Kimutai R,Edwards T,Hailu A

    更新日期:2014-01-16 00:00:00

  • Plasmodium vivax but Not Plasmodium falciparum Blood-Stage Infection in Humans Is Associated with the Expansion of a CD8+ T Cell Population with Cytotoxic Potential.

    abstract::P. vivax and P. falciparum parasites display different tropism for host cells and induce very different clinical symptoms and pathology, suggesting that the immune responses required for protection may differ between these two species. However, no study has qualitatively compared the immune responses to P. falciparum ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 临床试验,杂志文章


    authors: Burel JG,Apte SH,McCarthy JS,Doolan DL

    更新日期:2016-12-08 00:00:00

  • Insights into the sand fly saliva: Blood-feeding and immune interactions between sand flies, hosts, and Leishmania.

    abstract:BACKGROUND:Leishmaniases are parasitic diseases present worldwide that are transmitted to the vertebrate host by the bite of an infected sand fly during a blood feeding. Phlebotomine sand flies inoculate into the mammalian host Leishmania parasites embedded in promastigote secretory gel (PSG) with saliva, which is comp...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Lestinova T,Rohousova I,Sima M,de Oliveira CI,Volf P

    更新日期:2017-07-13 00:00:00

  • Adding MASP1 to the lectin pathway-Leprosy association puzzle: Hints from gene polymorphisms and protein levels.

    abstract:BACKGROUND:Deposition of complement factors on Mycobacterium leprae may enhance phagocytosis. Such deposition may occur through the lectin pathway of complement. Three proteins of the lectin pathway are produced from the gene MASP1: Mannan-binding lectin-associated serine protease 1 (MASP-1) and MASP-3 and mannan-bindi...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Weinschutz Mendes H,Boldt ABW,von Rosen Seeling Stahlke E,Jensenius JC,Thiel S,Messias-Reason IJT

    更新日期:2020-04-02 00:00:00

  • Leptospira Interrogans induces fibrosis in the mouse kidney through Inos-dependent, TLR- and NLR-independent signaling pathways.

    abstract:BACKGROUND:Leptospira (L.) interrogans are bacteria responsible for a worldwide reemerging zoonosis. Rodents carry L. interrogans asymptomatically in their kidneys and excrete bacteria in the urine, contaminating the environment. Humans get infected through skin contact and develop a mild or severe leptospirosis that m...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Fanton d'Andon M,Quellard N,Fernandez B,Ratet G,Lacroix-Lamandé S,Vandewalle A,Boneca IG,Goujon JM,Werts C

    更新日期:2014-01-30 00:00:00

  • The incubation period of Buruli ulcer (Mycobacterium ulcerans infection).

    abstract:INTRODUCTION:Buruli Ulcer (BU) is caused by the environmental microbe Mycobacterium ulcerans. Despite unclear transmission, contact with a BU endemic region is the key known risk factor. In Victoria, Australia, where endemic areas have been carefully mapped, we aimed to estimate the Incubation Period (IP) of BU by inte...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Trubiano JA,Lavender CJ,Fyfe JA,Bittmann S,Johnson PD

    更新日期:2013-10-03 00:00:00

  • Correction: The spectrum of neurological disease associated with Zika and chikungunya viruses in adults in Rio de Janeiro, Brazil: A case series.

    abstract::[This corrects the article DOI: 10.1371/journal.pntd.0006212.]. ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,已发布勘误


    authors: PLOS Neglected Tropical Diseases Staff.

    更新日期:2019-01-31 00:00:00

  • Family relationship, water contact and occurrence of Buruli ulcer in Benin.

    abstract:BACKGROUND:Mycobacterium ulcerans disease (Buruli ulcer) is the most widespread mycobacterial disease in the world after leprosy and tuberculosis. How M. ulcerans is introduced into the skin of humans remains unclear, but it appears that individuals living in the same environment may have different susceptibilities. O...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Sopoh GE,Barogui YT,Johnson RC,Dossou AD,Makoutodé M,Anagonou SY,Kestens L,Portaels F

    更新日期:2010-07-13 00:00:00

  • Schistosoma mansoni Infections in young children: when are schistosome antigens in urine, eggs in stool and antibodies to eggs first detectable?

    abstract:BACKGROUND:in uganda, control of intestinal schistosomiasis with preventive chemotherapy is typically focused towards treatment of school-aged children; the needs of younger children are presently being investigated as in lakeshore communities very young children can be infected. In the context of future epidemiologica...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Stothard JR,Sousa-Figueiredo JC,Betson M,Adriko M,Arinaitwe M,Rowell C,Besiyge F,Kabatereine NB

    更新日期:2011-01-04 00:00:00

  • Bacterial community composition in the salivary glands of triatomines (Hemiptera: Reduviidae).

    abstract:BACKGROUND:Chagas disease is caused by the parasite Trypanosoma cruzi and is transmitted through triatomines (Hemiptera: Reduviidae). In the last year, many studies of triatomine gut microbiota have outlined its potential role in modulating vector competence. However, little is known about the microbiota present in the...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Lima MS,Laport MS,Lorosa ES,Jurberg J,Dos Santos KRN,da Silva Neto MAC,Rachid CTCDC,Atella GC

    更新日期:2018-09-13 00:00:00

  • Animal reservoir, natural and socioeconomic variations and the transmission of hemorrhagic fever with renal syndrome in Chenzhou, China, 2006-2010.

    abstract:BACKGROUND:China has the highest incidence of hemorrhagic fever with renal syndrome (HFRS) worldwide. Reported cases account for 90% of the total number of global cases. By 2010, approximately 1.4 million HFRS cases had been reported in China. This study aimed to explore the effect of the rodent reservoir, and natural ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Xiao H,Tian HY,Gao LD,Liu HN,Duan LS,Basta N,Cazelles B,Li XJ,Lin XL,Wu HW,Chen BY,Yang HS,Xu B,Grenfell B

    更新日期:2014-01-09 00:00:00

  • Epidemiological characterization of incident cases of Rickettsia infection in rural areas of Urabá region, Colombia.

    abstract:INTRODUCTION:Most of the studies related to rickettsial infection in Colombia are cross-sectional because of the challenge in conducting prospective studies on infectious disease that may have a difficult diagnosis. Although cross-sectional studies are essential to detect people exposed to rickettsiae, they are not sui...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Quintero Vélez JC,Aguirre-Acevedo DC,Rodas JD,Arboleda M,Troyo A,Vega Aguilar F,Osorio Quintero L,Rojas Arbeláez C

    更新日期:2018-10-31 00:00:00

  • Performance and safety of praziquantel for treatment of intestinal schistosomiasis in infants and preschool children.

    abstract:BACKGROUND:In 2012 the WHO formally recognised that infants and preschool children are at significant risk of schistosomiasis and qualify for treatment with praziquantel (PZQ). Targeted surveys determining both the performance and safety of this drug are now needed in endemic areas. We have formally assessed parasitolo...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,随机对照试验


    authors: Sousa-Figueiredo JC,Betson M,Atuhaire A,Arinaitwe M,Navaratnam AM,Kabatereine NB,Bickle Q,Stothard JR

    更新日期:2012-01-01 00:00:00

  • Effect of immunosuppressants on the parasite load developed in, and immune response to, visceral leishmaniasis: A comparative study in a mouse model.

    abstract::The increasing use of immunosuppressants in areas where visceral leishmaniasis (VL) is endemic has increased the number of people susceptible to developing more severe forms of the disease. Few studies have examined the quality of the immune response in immunosuppressed patients or experimental animals with VL. The pr...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Bernardo L,Solana JC,Romero-Kauss A,Sánchez C,Carrillo E,Moreno J

    更新日期:2021-02-01 00:00:00

  • Evaluation of Chikungunya diagnostic assays: differences in sensitivity of serology assays in two independent outbreaks.

    abstract:BACKGROUND:The sensitivity and specificity of two in-house MAC-ELISA assays were tested and compared with the performance of commercially-available CTK lateral flow rapid test and EUROIMMUN IFA assays for the detection of anti-Chikungunya virus (CHIKV) IgM. Each MAC-ELISA assay used a whole virus-based antigen derived ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Yap G,Pok KY,Lai YL,Hapuarachchi HC,Chow A,Leo YS,Tan LK,Ng LC

    更新日期:2010-07-20 00:00:00

  • Environmental methods for dengue vector control - A systematic review and meta-analysis.

    abstract:BACKGROUND:Vector control remains the primary method to prevent dengue infections. Environmental interventions represent sustainable and safe methods as there are limited risks of environmental contamination and toxicity. The objective of this study is to perform a systematic review and meta-analysis of the effectivene...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,meta分析


    authors: Buhler C,Winkler V,Runge-Ranzinger S,Boyce R,Horstick O

    更新日期:2019-07-11 00:00:00

  • Increased risk for Entamoeba histolytica infection and invasive amebiasis in HIV seropositive men who have sex with men in Taiwan.

    abstract:BACKGROUND:Incidence of Entamoeba histolytica infection and clinical manifestations and treatment response of invasive amebiasis (IA) in HIV-infected patients have rarely been investigated before. METHODOLOGY/PRINCIPAL FINDINGS:At the National Taiwan University Hospital, medical records of HIV-infected patients who re...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Hung CC,Ji DD,Sun HY,Lee YT,Hsu SY,Chang SY,Wu CH,Chan YH,Hsiao CF,Liu WC,Colebunders R

    更新日期:2008-02-27 00:00:00

  • Biodegradable nanocarriers coated with polymyxin B: Evaluation of leishmanicidal and antibacterial potential.

    abstract::Most treatments of leishmaniasis require hospitalization and present side effects or parasite resistance; innovations in drug formulation/reposition can overcome these barriers and must be pursued to increase therapeutic alternatives. Therefore, we tested polymyxin B (polB) potential to kill Leishmania amazonensis, ad...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Souza Ribeiro Costa J,Medeiros M,Yamashiro-Kanashiro EH,Rocha MC,Cotrim PC,Stephano MA,Lancellotti M,Tavares GD,Oliveira-Nascimento L

    更新日期:2019-05-01 00:00:00

  • Projecting the long-term impact of school- or community-based mass-treatment interventions for control of Schistosoma infection.

    abstract:BACKGROUND:Schistosomiasis remains a significant health burden in many areas of the world. Morbidity control, focused on limiting infection intensity through periodic delivery of anti-schistosomal medicines, is the thrust of current World Health Organization guidelines (2006) for reduction of Schistosoma-related diseas...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Wang X,Gurarie D,Mungai PL,Muchiri EM,Kitron U,King CH

    更新日期:2012-01-01 00:00:00

  • A household serosurvey to estimate the magnitude of a dengue outbreak in Mombasa, Kenya, 2013.

    abstract::Dengue appears to be endemic in Africa with a number of reported outbreaks. In February 2013, several individuals with dengue-like illnesses and negative malaria blood smears were identified in Mombasa, Kenya. Dengue was laboratory confirmed and an investigation was conducted to estimate the magnitude of local transmi...

    journal_title:PLoS neglected tropical diseases

    pub_type: 历史文章,杂志文章


    authors: Ellis EM,Neatherlin JC,Delorey M,Ochieng M,Mohamed AH,Mogeni DO,Hunsperger E,Patta S,Gikunju S,Waiboic L,Fields B,Ofula V,Konongoi SL,Torres-Velasquez B,Marano N,Sang R,Margolis HS,Montgomery JM,Tomashek KM

    更新日期:2015-04-29 00:00:00

  • Protein Translation Enzyme lysyl-tRNA Synthetase Presents a New Target for Drug Development against Causative Agents of Loiasis and Schistosomiasis.

    abstract::Helminth parasites are an assemblage of two major phyla of nematodes (also known as roundworms) and platyhelminths (also called flatworms). These parasites are a major human health burden, and infections caused by helminths are considered under neglected tropical diseases (NTDs). These infections are typified by limit...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Sharma A,Sharma M,Yogavel M,Sharma A

    更新日期:2016-11-02 00:00:00

  • Ticks as potential vectors of Mycobacterium leprae: Use of tick cell lines to culture the bacilli and generate transgenic strains.

    abstract::Leprosy is an infectious disease caused by Mycobacterium leprae and frequently resulting in irreversible deformities and disabilities. Ticks play an important role in infectious disease transmission due to their low host specificity, worldwide distribution, and the biological ability to support transovarial transmissi...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Ferreira JDS,Souza Oliveira DA,Santos JP,Ribeiro CCDU,Baêta BA,Teixeira RC,Neumann ADS,Rosa PS,Pessolani MCV,Moraes MO,Bechara GH,de Oliveira PL,Sorgine MHF,Suffys PN,Fontes ANB,Bell-Sakyi L,Fonseca AH,Lara FA

    更新日期:2018-12-19 00:00:00

  • Loss of cytoplasmic incompatibility in Wolbachia-infected Aedes aegypti under field conditions.

    abstract::Wolbachia bacteria are now being introduced into Aedes aegypti mosquito populations for dengue control. When Wolbachia infections are at a high frequency, they influence the local transmission of dengue by direct virus blocking as well as deleterious effects on vector mosquito populations. However, the effectiveness o...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Ross PA,Ritchie SA,Axford JK,Hoffmann AA

    更新日期:2019-04-19 00:00:00

  • A new approach to dengue fatal cases diagnosis: NS1 antigen capture in tissues.

    abstract:UNLABELLED:/ BACKGROUND:Dengue is the most important arthropod borne viral disease worldwide in terms of morbidity and mortality and is caused by any of the four serotypes of dengue virus (DENV-1 to 4). Brazil is responsible for approximately 80% of dengue cases in the Americas, and since the introduction of dengue in...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Lima Mda R,Nogueira RM,Schatzmayr HG,de Filippis AM,Limonta D,dos Santos FB

    更新日期:2011-05-03 00:00:00

  • Prevalence of signs of trachoma, ocular Chlamydia trachomatis infection and antibodies to Pgp3 in residents of Kiritimati Island, Kiribati.

    abstract:OBJECTIVE:In some Pacific Island countries, such as Solomon Islands and Fiji, active trachoma is common, but ocular Chlamydia trachomatis (Ct) infection and trachomatous trichiasis (TT) are rare. On Tarawa, the most populous Kiribati island, both the active trachoma sign "trachomatous inflammation-follicular" (TF) and ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Cama A,Müller A,Taoaba R,Butcher RMR,Itibita I,Migchelsen SJ,Kiauea T,Pickering H,Willis R,Roberts CH,Bakhtiari A,Le Mesurier RT,Alexander NDE,Martin DL,Tekeraoi R,Solomon AW,Global Trachoma Mapping Project.

    更新日期:2017-09-12 00:00:00