OVA66 promotes tumour angiogenesis and progression through enhancing autocrine VEGF-VEGFR2 signalling.


BACKGROUND:Activation of autocrine VEGF-VEGFR2 signalling in tumour cells activates cell proliferation, survival, and angiogenesis, all of which are crucial for tumour progression. Ovarian cancer-associated antigen 66 (OVA66) is now known to be overexpressed in multiple tumours and plays a role in tumour development, but the underlying mechanisms has not been fully investigated. METHODS:We employed ovarian and cervical cancer cells and mouse models to detect the role of OVA66 in angiogenesis, growth, and metastasis of cancer cells. Immunofluorescence and western blot were used to determine the function of OVA66 in regulating autocrine VEGF-VEGFR2 signalling. Immunohistochemistry and bioinformatics analysis were used to detect the correlation of OVA66 and VEGF expression. FINDINGS:OVA66 overexpression in the cancer cell lines promoted VEGF secretion, tumour growth and angiogenesis in vitro and in vivo. Conversely, shRNA-mediated OVA66 knockdown had the opposite effects. Mechanistically, OVA66 overexpression was found to boost an autocrine VEGF-VEGFR2 positive-feedback signalling loop in the tumour cells, leading to amplified effect of VEGF on tumour angiogenesis and proliferation and increased migration in vitro and in vivo, respectively. Finally, we identified a significant positive correlation between the expression levels of OVA66 and VEGF in ovarian and cervical cancer specimens, and found that OVA66 was significantly associated with advanced ovarian cancer. INTERPRETATION:We identify a novel function for OVA66 in regulating an autocrine VEGF-VEGFR2 feed-forward signalling loop that promotes tumour progression and angiogenesis. FUND: This work was supported by the National Natural Science Foundation of China (81602262); and Excellent Youth Scholar Program of Tongji University (2015KJ062).






Song F,Chen Q,Rao W,Zhang R,Wang Y,Ge H,Wei Q




Has Abstract


2019-03-01 00:00:00










  • Tissue Microbiome Profiling Identifies an Enrichment of Specific Enteric Bacteria in Opisthorchis viverrini Associated Cholangiocarcinoma.

    abstract::Cholangiocarcinoma (CCA) is the primary cancer of the bile duct system. The role of bile duct tissue microbiomes in CCA tumorigenesis is unestablished. To address this, sixty primary CCA tumors and matched normals, from both liver fluke (Opisthorchis viverrini) associated (OVa, n=28) and non-O. viverrini associated (n...


    pub_type: 杂志文章


    authors: Chng KR,Chan SH,Ng AHQ,Li C,Jusakul A,Bertrand D,Wilm A,Choo SP,Tan DMY,Lim KH,Soetinko R,Ong CK,Duda DG,Dima S,Popescu I,Wongkham C,Feng Z,Yeoh KG,Teh BT,Yongvanit P,Wongkham S,Bhudhisawasdi V,Khuntikeo N,T

    更新日期:2016-06-01 00:00:00

  • The neuropeptide calcitonin gene-related peptide alpha is essential for bone healing.

    abstract:BACKGROUND:Impaired fracture healing represents an ongoing clinical challenge, as treatment options remain limited. Calcitonin gene-related peptide (CGRP), a neuropeptide targeted by emerging anti-migraine drugs, is also expressed in sensory nerve fibres innervating bone tissue. METHOD:Bone healing following a femoral...


    pub_type: 杂志文章


    authors: Appelt J,Baranowsky A,Jahn D,Yorgan T,Köhli P,Otto E,Farahani SK,Graef F,Fuchs M,Herrera A,Amling M,Schinke T,Frosch KH,Duda GN,Tsitsilonis S,Keller J

    更新日期:2020-09-01 00:00:00

  • Impact of the host environment on the antitubercular action of pyrazinamide.

    abstract::Pyrazinamide remains the only drug in the tuberculosis pharmacopeia to drastically shorten first-line therapy from nine to six months. Due to its unparalleled ability to sterilize non-replicating bacilli and reduce relapse rates, PZA is expected to be irreplaceable in future therapies against tuberculosis. While the m...


    pub_type: 杂志文章,评审


    authors: Lamont EA,Baughn AD

    更新日期:2019-11-01 00:00:00

  • Contemporary update of overall prognosis and nomogram to predict individualized survival for Chinese patients with eyelid sebaceous carcinoma.

    abstract:BACKGROUND:The prognosis of Chinese patients with eyelid sebaceous carcinoma (SC) has not been updated for >3 decades. The prognostic predictors are multifactorial, and there is no validated prognostic model for eyelid SC. METHODS:This study included 238 consecutive patients with eyelid SC. All eligible patients were ...


    pub_type: 杂志文章


    authors: Zhou C,Shi Y,Chai P,Wu F,Xia W,He X,Shi Y,Huang H,Jia R,Fan X

    更新日期:2018-10-01 00:00:00

  • Sphingomyelin synthase 1 enhances BCR signaling to promote lupus-like autoimmune response.

    abstract:BACKGROUND:Sphingomyelin synthase 1 (SMS1) has been reported to participate in hepatitis and atherosclerosis. However, its role in autoimmune response is not clear. This study investigates the possible involvement of SMS1 in B-cell activation and lupus-like autoimmunity. METHODS:SMS1 knockout lupus-like animal model a...


    pub_type: 杂志文章


    authors: Wang C,Ming B,Wu X,Wu T,Cai S,Hu P,Tang J,Tan Z,Liu C,Zhong J,Zheng F,Dong L

    更新日期:2019-07-01 00:00:00

  • Heterologous Two-Dose Vaccination with Simian Adenovirus and Poxvirus Vectors Elicits Long-Lasting Cellular Immunity to Influenza Virus A in Healthy Adults.

    abstract:BACKGROUND:T-cell responses against highly conserved influenza antigens have been previously associated with protection. However, these immune responses are poorly maintained following recovery from influenza infection and are not boosted by inactivated influenza vaccines. We have previously demonstrated the safety and...


    pub_type: 杂志文章


    authors: Coughlan L,Sridhar S,Payne R,Edmans M,Milicic A,Venkatraman N,Lugonja B,Clifton L,Qi C,Folegatti PM,Lawrie AM,Roberts R,de Graaf H,Sukhtankar P,Faust SN,Lewis DJM,Lambe T,Hill A,Gilbert SC

    更新日期:2018-03-01 00:00:00

  • MicroRNA-196a links human body fat distribution to adipose tissue extracellular matrix composition.

    abstract:BACKGROUND:Abdominal fat mass is associated with metabolic risk whilst gluteal femoral fat is paradoxically protective. MicroRNAs are known to be necessary for adipose tissue formation and function but their role in regulating human fat distribution remains largely unexplored. METHODS:An initial microarray screen of a...


    pub_type: 杂志文章


    authors: Hilton C,Neville MJ,Wittemans LBL,Todorcevic M,Pinnick KE,Pulit SL,Luan J,Kulyté A,Dahlman I,Wareham NJ,Lotta LA,Arner P,Lindgren CM,Langenberg C,Karpe F

    更新日期:2019-06-01 00:00:00

  • HIV Transmission Networks in the San Diego-Tijuana Border Region.

    abstract:BACKGROUND:HIV sequence data can be used to reconstruct local transmission networks. Along international borders, like the San Diego-Tijuana region, understanding the dynamics of HIV transmission across reported risks, racial/ethnic groups, and geography can help direct effective prevention efforts on both sides of the...


    pub_type: 杂志文章


    authors: Mehta SR,Wertheim JO,Brouwer KC,Wagner KD,Chaillon A,Strathdee S,Patterson TL,Rangel MG,Vargas M,Murrell B,Garfein R,Little SJ,Smith DM

    更新日期:2015-07-18 00:00:00

  • Large Isoform of Mammalian Relative of DnaJ is a Major Determinant of Human Susceptibility to HIV-1 Infection.

    abstract::Individual differences in susceptibility to human immunodeficiency virus type 1 (HIV-1) infection have been of interest for decades. We aimed to determine the contribution of large isoform of Mammalian DnaJ (MRJ-L), a HIV-1 Vpr-interacting cellular protein, to this natural variation. Expression of MRJ-L in monocyte-de...


    pub_type: 杂志文章


    authors: Chiang YP,Sheng WH,Shao PL,Chi YH,Chen YM,Huang SW,Shih HM,Chang LY,Lu CY,Chang SC,Hung CC,Huang LM

    更新日期:2014-10-07 00:00:00

  • Next Generation DNA Sequencing of Tissues from Infected Diabetic Foot Ulcers.

    abstract::We used next generation DNA sequencing to profile the microbiome of infected Diabetic Foot Ulcers (DFUs). The microbiota was correlated to clinical parameters and treatment outcomes to determine if directed antimicrobial therapy based on conventional microbiological cultures are relevant based on genomic analysis. Pat...


    pub_type: 杂志文章


    authors: Malone M,Johani K,Jensen SO,Gosbell IB,Dickson HG,Hu H,Vickery K

    更新日期:2017-07-01 00:00:00

  • Flecainide in Amyotrophic Lateral Sclerosis as a Neuroprotective Strategy (FANS): A Randomized Placebo-Controlled Trial.

    abstract:BACKGROUND:Abnormalities in membrane excitability and Na(+) channel function are characteristic of amyotrophic lateral sclerosis (ALS). We aimed to examine the neuroprotective potential, safety and tolerability of the Na(+) channel blocker and membrane stabiliser flecainide in ALS. METHODS:A double-blind, placebo-cont...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Park SB,Vucic S,Cheah BC,Lin CS,Kirby A,Mann KP,Zoing MC,Winhammar J,Kiernan MC

    更新日期:2015-12-01 00:00:00

  • Integrated epigenomic profiling reveals endogenous retrovirus reactivation in renal cell carcinoma.

    abstract:BACKGROUND:Transcriptional dysregulation drives cancer formation but the underlying mechanisms are still poorly understood. Renal cell carcinoma (RCC) is the most common malignant kidney tumor which canonically activates the hypoxia-inducible transcription factor (HIF) pathway. Despite intensive study, novel therapeuti...


    pub_type: 杂志文章


    authors: Siebenthall KT,Miller CP,Vierstra JD,Mathieu J,Tretiakova M,Reynolds A,Sandstrom R,Rynes E,Haugen E,Johnson A,Nelson J,Bates D,Diegel M,Dunn D,Frerker M,Buckley M,Kaul R,Zheng Y,Himmelfarb J,Ruohola-Baker H,Akiles

    更新日期:2019-03-01 00:00:00

  • Transcriptional and clonal characterization of B cell plasmablast diversity following primary and secondary natural DENV infection.

    abstract::Antibody-mediated humoral immunity is thought to play a central role in mediating the immunopathogenesis of acute DENV infection, but limited data are available on the diversity, specificity, and functionality of the antibody response at the molecular level elicited by primary or secondary DENV infection. In order to ...


    pub_type: 杂志文章


    authors: Waickman AT,Gromowski GD,Rutvisuttinunt W,Li T,Siegfried H,Victor K,Kuklis C,Gomootsukavadee M,McCracken MK,Gabriel B,Mathew A,Grinyo I Escuer A,Fouch ME,Liang J,Fernandez S,Davidson E,Doranz BJ,Srikiatkhachorn A,Endy

    更新日期:2020-04-01 00:00:00

  • Memory B Cells are Major Targets for Effective Immunotherapy in Relapsing Multiple Sclerosis.

    abstract::Although multiple sclerosis (MS) is considered to be a CD4, Th17-mediated autoimmune disease, supportive evidence is perhaps circumstantial, often based on animal studies, and is questioned by the perceived failure of CD4-depleting antibodies to control relapsing MS. Therefore, it was interestingly to find that curren...


    pub_type: 杂志文章,评审


    authors: Baker D,Marta M,Pryce G,Giovannoni G,Schmierer K

    更新日期:2017-02-01 00:00:00

  • The Effect of Mutations on Drug Sensitivity and Kinase Activity of Fibroblast Growth Factor Receptors: A Combined Experimental and Theoretical Study.

    abstract::Fibroblast growth factor receptors (FGFRs) are recognized therapeutic targets in cancer. We here describe insights underpinning the impact of mutations on FGFR1 and FGFR3 kinase activity and drug efficacy, using a combination of computational calculations and experimental approaches including cellular studies, X-ray c...


    pub_type: 杂志文章


    authors: Bunney TD,Wan S,Thiyagarajan N,Sutto L,Williams SV,Ashford P,Koss H,Knowles MA,Gervasio FL,Coveney PV,Katan M

    更新日期:2015-03-01 00:00:00

  • Reporting of Cardiovascular Medical Device Adverse Events to Pharmaceuticals and Medical Devices Agency, Japan.

    abstract:BACKGROUND:Marketing authorization holders (MAHs) are obligated to report adverse events (AEs) within 15 days (some cases 30 days) to the Pharmaceuticals and Medical Devices Agency (PMDA) of Japan. METHODS:To analyze the timeliness of AE reporting to the PMDA, 6610 reports for five categories of cardiovascular devices...


    pub_type: 杂志文章


    authors: Handa N,Ishii K,Matsui Y,Ando Y

    更新日期:2015-07-10 00:00:00

  • The association of α4β7 expression with HIV acquisition and disease progression in people who inject drugs and men who have sex with men: Case control studies.

    abstract:BACKGROUND:α4β7 is a gut-homing integrin heterodimer that can act as a non-essential binding molecule for HIV. A previous study in heterosexual African women found that individuals with higher proportions of α4β7 expressing CD4+ T cells were more likely to become infected with HIV, as well as present with faster diseas...


    pub_type: 杂志文章


    authors: Martin AR,Patel EU,Kirby C,Astemborski J,Kirk GD,Mehta SH,Marshall K,Janes H,Clayton A,Corey L,Hammer SM,Sobieszczyk ME,Arthos J,Cicala C,Redd AD,Quinn TC

    更新日期:2020-12-01 00:00:00

  • Downregulation of circRNA DMNT3B contributes to diabetic retinal vascular dysfunction through targeting miR-20b-5p and BAMBI.

    abstract:BACKGROUND:Diabetic retinopathy, a vascular complication of diabetes mellitus, is the leading cause of visual impairment and blindness. circRNAs act as competing endogenous RNA, sponging target miRNA and thus influencing mRNA expression in vascular diseases. We investigated whether and how circDNMT3B is involved in ret...


    pub_type: 杂志文章


    authors: Zhu K,Hu X,Chen H,Li F,Yin N,Liu AL,Shan K,Qin YW,Huang X,Chang Q,Xu GZ,Wang Z

    更新日期:2019-11-01 00:00:00

  • Quantification, epitope mapping and genotype cross-reactivity of hepatitis B preS-specific antibodies in subjects vaccinated with different dosage regimens of BM32.

    abstract:BACKGROUND:Chronic hepatitis B virus (HBV) infections are a global health problem. There is a need for therapeutic strategies blocking continuous infection of liver cells. The grass pollen allergy vaccine BM32 containing the preS domain of the large HBV surface protein (LHBs) as immunogenic carrier induced IgG antibodi...


    pub_type: 杂志文章


    authors: Tulaeva I,Cornelius C,Zieglmayer P,Zieglmayer R,Schmutz R,Lemell P,Weber M,Focke-Tejkl M,Karaulov A,Henning R,Valenta R

    更新日期:2020-09-01 00:00:00

  • Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7.

    abstract::Rabbits (Oryctolagus cuniculus) have been the very frequently used as animal models in the study of human lipid metabolism and atherosclerosis, because they have similar lipoprotein metabolism to humans. Most of hyperlipidemia and atherosclerosis rabbit models are produced by feeding rabbits a high-cholesterol diet. G...


    pub_type: 杂志文章


    authors: Lu R,Yuan T,Wang Y,Zhang T,Yuan Y,Wu D,Zhou M,He Z,Lu Y,Chen Y,Fan J,Liang J,Cheng Y

    更新日期:2018-10-01 00:00:00

  • Surgery, neuroinflammation and cognitive impairment.

    abstract::Trauma experienced during surgery can contribute to the development of a systemic inflammatory response that can cause multi-organ dysfunction or even failure. Post-surgical neuroinflammation is a documented phenomenon that results in synaptic impairment, neuronal dysfunction and death, and impaired neurogenesis. Vari...


    pub_type: 杂志文章,评审


    authors: Alam A,Hana Z,Jin Z,Suen KC,Ma D

    更新日期:2018-11-01 00:00:00

  • Anthropogenic Carbon Nanotubes Found in the Airways of Parisian Children.

    abstract::Compelling evidence shows that fine particulate matters (PMs) from air pollution penetrate lower airways and are associated with adverse health effects even within concentrations below those recommended by the WHO. A paper reported a dose-dependent link between carbon content in alveolar macrophages (assessed only by ...


    pub_type: 杂志文章


    authors: Kolosnjaj-Tabi J,Just J,Hartman KB,Laoudi Y,Boudjemaa S,Alloyeau D,Szwarc H,Wilson LJ,Moussa F

    更新日期:2015-10-09 00:00:00

  • Peptides of tetraspanin oncoprotein CD151 trigger active immunity against primary tumour and experimental lung metastasis.

    abstract:BACKGROUND:Active immunotherapy is an effective, long-lasting, cheap, and safe approach to suppress cancer progression; however, the key issue is to develop appropriate tumour vaccines. Oncoproteins are up-regulated under various stress conditions and promote cell survival. Oncoproteins and their immunogenic domains co...


    pub_type: 杂志文章


    authors: Lin W,Liu J,Chen J,Li J,Qiu S,Ma J,Lin X,Zhang L,Wu J

    更新日期:2019-11-01 00:00:00

  • Chlamydia trachomatis Prevents Apoptosis Via Activation of PDPK1-MYC and Enhanced Mitochondrial Binding of Hexokinase II.

    abstract::The intracellular human bacterial pathogen Chlamydia trachomatis pursues effective strategies to protect infected cells against death-inducing stimuli. Here, we show that Chlamydia trachomatis infection evokes 3-phosphoinositide-dependent protein kinase-1 (PDPK1) signaling to ensure the completion of its developmental...


    pub_type: 杂志文章


    authors: Al-Zeer MA,Xavier A,Abu Lubad M,Sigulla J,Kessler M,Hurwitz R,Meyer TF

    更新日期:2017-09-01 00:00:00

  • Liver Dysfunction during Pregnancy and Its Association of With Preterm Birth in China: A Prospective Cohort Study.

    abstract:BACKGROUND:Liver dysfunction is common in pregnancy but its association with adverse pregnancy outcomes such as preterm birth (PTB) remains unclear. METHODS:A prospective cohort of HBV-infected or uninfected pregnant women attending antenatal care was recruited at Nantong Maternal and Child Health Hospital between Jan...


    pub_type: 杂志文章


    authors: Zhuang X,Cui AM,Wang Q,Cheng XY,Shen Y,Cai WH,Li HB,Zhang S,Qin G

    更新日期:2017-12-01 00:00:00

  • Anti-Depressant Fluoxetine Reveals its Therapeutic Effect Via Astrocytes.

    abstract::Although psychotropic drugs act on neurons and glial cells, how glia respond, and whether glial responses are involved in therapeutic effects are poorly understood. Here, we show that fluoxetine (FLX), an anti-depressant, mediates its anti-depressive effect by increasing the gliotransmission of ATP. FLX increased ATP ...


    pub_type: 杂志文章


    authors: Kinoshita M,Hirayama Y,Fujishita K,Shibata K,Shinozaki Y,Shigetomi E,Takeda A,Le HPN,Hayashi H,Hiasa M,Moriyama Y,Ikenaka K,Tanaka KF,Koizumi S

    更新日期:2018-06-01 00:00:00

  • Image-guided surgery of head and neck carcinoma in rabbit models by intra-operatively defining tumour-infiltrated margins and metastatic lymph nodes.

    abstract:BACKGROUND:The infiltrative nature and lymphatic metastasis of head and neck squamous cell carcinoma (HNSCC) are the main reasons leading to its poor prognosis. METHODS:A multimodal surface-enhanced resonance Raman spectroscopy (SERRS) and magnetic resonance (MR) nanoprobe, in which paramagnetic chelators and heptamet...


    pub_type: 杂志文章


    authors: Sun P,Zhang Y,Li K,Wang C,Zeng F,Zhu J,Wu Y,Tao X

    更新日期:2019-12-01 00:00:00

  • A panel of five plasma proteins for the early diagnosis of hepatitis B virus-related hepatocellular carcinoma in individuals at risk.

    abstract:BACKGROUND:To improve the early diagnosis of hepatocellular carcinoma (HCC), more effective diagnostic biomarkers are needed. A combination of biomarkers is reported to distinguish individuals with early-stage HCC from at-risk individuals. METHODS:Participants in this study were recruited from six hospitals in China. ...


    pub_type: 杂志文章,多中心研究


    authors: Cheng K,Shi J,Liu Z,Jia Y,Qin Q,Zhang H,Wan S,Niu Z,Lu L,Sun J,Xue J,Lu C,Wei X,Guo L,Zhang F,Zhou D,Tang Y,Hu Y,Huang Y,Chen Y,Lau WY,Cheng S,Liu S

    更新日期:2020-02-01 00:00:00

  • Diagnostic Performance of Plasma DNA Methylation Profiles in Lung Cancer, Pulmonary Fibrosis and COPD.

    abstract::Disease-specific alterations of the cell-free DNA methylation status are frequently found in serum samples and are currently considered to be suitable biomarkers. Candidate markers were identified by bisulfite conversion-based genome-wide methylation screening of lung tissue from lung cancer, fibrotic ILD, and COPD. c...


    pub_type: 杂志文章,随机对照试验


    authors: Wielscher M,Vierlinger K,Kegler U,Ziesche R,Gsur A,Weinhäusel A

    更新日期:2015-07-02 00:00:00

  • A clade of SARS-CoV-2 viruses associated with lower viral loads in patient upper airways.

    abstract:BACKGROUND:The rapid spread of SARS-CoV-2, the causative agent of Coronavirus disease 2019 (COVID-19), has been accompanied by the emergence of distinct viral clades, though their clinical significance remains unclear. Here, we aimed to investigate the phylogenetic characteristics of SARS-CoV-2 infections in Chicago, I...


    pub_type: 临床试验,杂志文章


    authors: Lorenzo-Redondo R,Nam HH,Roberts SC,Simons LM,Jennings LJ,Qi C,Achenbach CJ,Hauser AR,Ison MG,Hultquist JF,Ozer EA

    更新日期:2020-12-01 00:00:00