DR region specific antibody ameliorated but ouabain worsened renal injury in nephrectomized rats through regulating Na,K-ATPase mediated signaling pathways.


:Reduced Na+-K+-ATPase function is reported in various renal diseases. This implies that increase of Na+-K+-ATPase function may be a new target in treatment of renal injury. We previously reported that Na+-K+-ATPase was stabilized by DRm217, a specific antibody against DR region of Na+-K+-ATPase. In this study, we compared the protective effect of DRm217 and ouabain on kidney in a chronic kidney disease rat model and investigated the mechanism under it. We found that DRm217 improved renal function, alleviated glomerulus atrophy, inhibited renal tubular cells apoptosis, tubulointerstitial injury and renal fibrosis in 5/6 nephrectomized rats. Contrary to DRm217, ouabain worsened renal damage. Activated Na+-K+-ATPase /Src signaling pathway, increased oxidant stress and activated inflammasome were responsible for nephrectomized or ouabain-induced renal injury. DRm217 inhibited Na+-K+-ATPase /Src signaling pathway, retarded oxidant stress, suppressed inflammasome activation, and improved renal function, suggesting a novel approach to prevent renal damage.


Aging (Albany NY)




Wang J,Ullah SH,Li M,Zhang M,Zhang F,Zheng J,Yan X




Has Abstract


2019-02-26 00:00:00














  • Loss of macroH2A1 decreases mitochondrial metabolism and reduces the aggressiveness of uveal melanoma cells.

    abstract::Uveal melanoma (UM) is the most common primary intraocular tumour in adults. The most accurate prognostic factor of UM is classification by gene expression profiling. Currently, the role of epigenetics is much less defined compared to genetic mechanisms. We recently showed a strong prognostic role of the expression le...


    pub_type: 杂志文章


    authors: Giallongo S,Di Rosa M,Caltabiano R,Longhitano L,Reibaldi M,Distefano A,Lo Re O,Amorini AM,Puzzo L,Salvatorelli L,Palmucci S,Tibullo D,Russo A,Longo A,Lazzarino G,Li Volti G,Vinciguerra M

    更新日期:2020-05-12 00:00:00

  • Hsa_circ_0000199 facilitates chemo-tolerance of triple-negative breast cancer by interfering with miR-206/613-led PI3K/Akt/mTOR signaling.

    abstract::Increasing attentions have been paid to the role of circRNAs in the etiology of triple-negative breast cancer (TNBC), and we strived to figure out the association of circRNA AKT3/miRNA axis with TNBC chemo-resistance. Altogether 207 BC patients were divided into TNBC group (n=83) and non-TNBC group (n=124), and MCF-10...


    pub_type: 杂志文章


    authors: Li H,Xu W,Xia Z,Liu W,Pan G,Ding J,Li J,Wang J,Xie X,Jiang D

    更新日期:2021-01-20 00:00:00

  • Genes associated with Type 2 Diabetes and vascular complications.

    abstract::Type 2 Diabetes (T2D) is a chronic disease associated with a number of micro- and macrovascular complications that increase the morbidity and mortality of patients. The risk of diabetic complications has a strong genetic component. To this end, we sought to evaluate the association of 40 single nucleotide polymorphism...


    pub_type: 杂志文章


    authors: Montesanto A,Bonfigli AR,Crocco P,Garagnani P,De Luca M,Boemi M,Marasco E,Pirazzini C,Giuliani C,Franceschi C,Passarino G,Testa R,Olivieri F,Rose G

    更新日期:2018-02-04 00:00:00

  • RNA interactions in right ventricular dysfunction induced type II cardiorenal syndrome.

    abstract::Right ventricular (RV) dysfunction induced type II cardiorenal syndrome (CRS) has a high mortality rate, but little attention has been paid to this disease, and its unique molecular characteristics remain unclear. This study aims to investigate the transcriptomic expression profile in this disease and identify key RNA...


    pub_type: 杂志文章


    authors: Chen K,Huang X,Xie D,Shen M,Lin H,Zhu Y,Ma S,Zheng C,Chen L,Liu Y,Liao W,Bin J,Liao Y

    更新日期:2021-01-20 00:00:00

  • TAp73 regulates ATP7A: possible implications for ageing-related diseases.

    abstract::The p53 family member p73 controls a wide range of cellular function. Deletion of p73 in mice results in increased tumorigenesis, infertility, neurological defects and altered immune system. Despite the extensive effort directed to define the molecular underlying mechanism of p73 function a clear definition of its tra...


    pub_type: 杂志文章


    authors: Lopriore P,Capitanio N,Panatta E,Di Daniele N,Gambacurta A,Melino G,Amelio I

    更新日期:2018-12-08 00:00:00

  • The role of commensal microbes in the lifespan of Drosophila melanogaster.

    abstract::Commensal microbes have mutualistic relationships with their host and mainly live in the host intestine. There are many studies on the relationships between commensal microbes and host physiology. However, there are inconsistent results on the effects of commensal microbes on host lifespan. To clarify this controversy...


    pub_type: 杂志文章


    authors: Lee HY,Lee SH,Lee JH,Lee WJ,Min KJ

    更新日期:2019-07-12 00:00:00

  • Birth mass is the key to understanding the negative correlation between lifespan and body size in dogs.

    abstract::Larger dog breeds live shorter than the smaller ones, opposite of the mass-lifespan relationship observed across mammalian species. Here we use data from 90 dog breeds and a theoretical model based on the first principles of energy conservation and life history tradeoffs to explain the negative correlation between lon...


    pub_type: 杂志文章


    authors: Fan R,Olbricht G,Baker X,Hou C

    更新日期:2016-12-08 00:00:00

  • Annotation of susceptibility SNPs associated with atrial fibrillation.

    abstract:OBJECTIVE:Genome-wide association studies (GWAS) and the candidate gene based association studies have identified a panel of variants associated with atrial fibrillation (AF), however, most of the identified single nucleotide polymorphisms (SNPs) were found located within intergenic or intronic genomic regions, and whe...


    pub_type: 杂志文章


    authors: Xu C,Zhang R,Xia Y,Xiong L,Yang W,Wang P

    更新日期:2020-09-09 00:00:00

  • Extracellular vesicles derived from microRNA-150-5p-overexpressing mesenchymal stem cells protect rat hearts against ischemia/reperfusion.

    abstract::An intriguing area of research has demonstrated the ability of extracellular vesicles (EVs) as biological vehicles for microRNAs (miRNAs) transfer. Mesenchymal stem cells (MSCs) produce large amounts of EVs. Rat models of ischemia/reperfusion (I/R) were established to explore the expression profile of thioredoxin-inte...


    pub_type: 杂志文章


    authors: Ou H,Teng H,Qin Y,Luo X,Yang P,Zhang W,Chen W,Lv D,Tang H

    更新日期:2020-07-13 00:00:00

  • White light emitting diode induces autophagy in hippocampal neuron cells through GSK-3-mediated GR and RORα pathways.

    abstract::Autophagy plays an important role in cell survival under diverse stress conditions. Here, we show that white LED light exposure for 24 h significantly activated autophagy-related genes and increased autophagosome formation in hippocampal neural cells (HT-22). Concurrently, the rhythmic pattern of clock-related gene ex...


    pub_type: 杂志文章


    authors: Yang Y,Jia Y,Sun Q,Dong H,Zhao R

    更新日期:2019-03-28 00:00:00

  • IL-21 mediates microRNA-423-5p /claudin-5 signal pathway and intestinal barrier function in inflammatory bowel disease.

    abstract::Inflammatory bowel disease (IBD) is a group of chronic and recurrent nonspecific inflammatory disorders, including Crohn's disease (CD) and ulcerative colitis (UC). Due to the persistent inflammation of intestinal mucosa caused by immune disorders, barrier dysfunction may be an essential cause of the pathogenesis of I...


    pub_type: 杂志文章


    authors: Wang M,Guo J,Zhao YQ,Wang JP

    更新日期:2020-08-28 00:00:00

  • DNA methylation perturbations may link altered development and aging in the lung.

    abstract::Fetal perturbations in DNA methylation during lung development may reveal insights into the enduring impacts on adult lung health and disease during aging that have not been explored altogether before. We studied the association between genome-wide DNA-methylation and post-conception age in fetal-lung (n=78, 42 expose...


    pub_type: 杂志文章


    authors: Kachroo P,Morrow JD,Vyhlidal CA,Gaedigk R,Silverman EK,Weiss ST,Tantisira KG,DeMeo DL

    更新日期:2021-01-19 00:00:00

  • Circ_0006332 promotes growth and progression of bladder cancer by modulating MYBL2 expression via miR-143.

    abstract::In this study, we analyzed the role of circular RNAs in the growth and progression of bladder cancer. Direct Sanger sequencing and quantitative RT-PCR analysis showed that circ_0006332 was significantly upregulated in bladder cancer tissues. Sequencing analysis showed that circ_0006332 is generated from splicing of ex...


    pub_type: 杂志文章


    authors: Li M,Liu Y,Liu J,Li W,Li N,Xue D,Zhang X,Wang P

    更新日期:2019-11-22 00:00:00

  • Role of apoptosis in disease.

    abstract::Since the initial description of apoptosis, a number of different forms of cell death have been described. In this review we will focus on classic caspase-dependent apoptosis and its variations that contribute to diseases. Over fifty years of research have clarified molecular mechanisms involved in apoptotic signaling...


    pub_type: 杂志文章,评审


    authors: Favaloro B,Allocati N,Graziano V,Di Ilio C,De Laurenzi V

    更新日期:2012-05-01 00:00:00

  • Characterization of variations in IL23A and IL23R genes: possible roles in multiple sclerosis and other neuroinflammatory demyelinating diseases.

    abstract::Multiple sclerosis is among the most serious inflammatory demyelinating diseases (IDD). Interleukin-23A (IL23A) regulates and coordinates the activities of immune cells by interacting with its receptor IL23R and plays key roles in the pathogenesis of immune inflammatory diseases. IDD, deemed to be a kind of autoimmune...


    pub_type: 杂志文章


    authors: Li FF,Zhu XD,Yan P,Jin MH,Yue H,Zhang Q,Fu J,Liu SL

    更新日期:2016-11-26 00:00:00

  • Salvia miltiorrhiza bunge increases estrogen level without side effects on reproductive tissues in immature/ovariectomized mice.

    abstract::Salvia miltiorrhiza bunge(SM) is a popular herb for alleviating menopausal symptoms, although the scientific evidence of applying SM to estrogen replacement therapy is limited. In this study, we characterized the estrogenic activity of SM using in vivo models of immature and ovariectomized (OVX) mice and performed in ...


    pub_type: 杂志文章


    authors: Xu Y,Chen T,Li X,Qu YK,An JN,Zheng HX,Zhang ZJ,Lin N

    更新日期:2016-12-20 00:00:00

  • The senescence-associated secretory phenotype (SASP) from mesenchymal stromal cells impairs growth of immortalized prostate cells but has no effect on metastatic prostatic cancer cells.

    abstract::Senescent cells secrete inflammatory cytokines, proteases, and other factors, which are indicated as senescence-associated secretory phenotype (SASP). There are contrasting studies on the role of the SASP in cancer. Studies suggested that cancer cells may misuse the senescent secretome for their growth. Other investig...


    pub_type: 杂志文章


    authors: Alessio N,Aprile D,Squillaro T,Di Bernardo G,Finicelli M,Melone MA,Peluso G,Galderisi U

    更新日期:2019-08-14 00:00:00

  • Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice.

    abstract::Sarcopenia is the degenerative loss of muscle mass and strength with aging. Although a role of mitochondrial metabolism in muscle function and in the development of many diseases has been described, the role of mitochondrial topology and dynamics in the process of muscle aging is not fully understood. This work shows ...


    pub_type: 杂志文章


    authors: Del Campo A,Contreras-Hernández I,Castro-Sepúlveda M,Campos CA,Figueroa R,Tevy MF,Eisner V,Casas M,Jaimovich E

    更新日期:2018-01-04 00:00:00

  • Establishing a density-based method to separate proliferating and senescent cells from bone marrow stromal cells.

    abstract::To assist in the study of cellular aging, we established a new method of enriching physiologically aged bone marrow stromal cells (BMSCs) in animals of any age using a Percoll density gradient centrifugation technique. BMSCs from mice 2 months of age were isolated, and their cellular age determined (over 80% Scal-1+ C...


    pub_type: 杂志文章


    authors: Xu F,Zhang Q,Wang H

    更新日期:2020-07-25 00:00:00

  • Effects of enriched branched-chain amino acid supplementation on sarcopenia.

    abstract::To evaluate the effects of short-term administration of enriched branched-chain amino acids (BCAAs) on subjects with pre-sarcopenia or sarcopenia, our quasi-experimental study enrolled 33 subjects (12 pre-sarcopenia/21 sarcopenia; 6 men/27 women; mean age 66.6 ± 10.3 years) to take one sachet (3.6 g) of enriched BCAA ...


    pub_type: 杂志文章


    authors: Ko CH,Wu SJ,Wang ST,Chang YF,Chang CS,Kuan TS,Chuang HY,Chang CM,Chou W,Wu CH

    更新日期:2020-07-26 00:00:00

  • Age-associated epigenetic modifications in human DNA increase its immunogenicity.

    abstract::Chronic inflammation, increased reactivity to self-antigens and incidences of cancer are hallmarks of aging. However, the underlying mechanisms are not well understood. Age-associated alterations in the DNA either due to oxidative damage, defects in DNA repair or epigenetic modifications such as methylation that lead ...


    pub_type: 杂志文章


    authors: Agrawal A,Tay J,Yang GE,Agrawal S,Gupta S

    更新日期:2010-03-20 00:00:00

  • The clinicopathological characteristics and genetic alterations between younger and older gastric cancer patients with curative surgery.

    abstract::Few reports have investigated different genetic alterations according to age in various cancers. In total, 1749 GC patients receiving curative surgery were enrolled. The clinicopathological features, and prognoses were compared between younger (<65 years) and older (≥65 years) patients. Genetic mutations were analyzed...


    pub_type: 杂志文章


    authors: Wu CW,Chen MH,Huang KH,Chang SC,Fang WL,Lin CH,Chao Y,Lo SS,Li AF,Shyr YM

    更新日期:2020-08-18 00:00:00

  • RWR-algorithm-based dissection of microRNA-506-3p and microRNA-140-5p as radiosensitive biomarkers in colorectal cancer.

    abstract::Radiotherapy resistance is one of the main causes for treatment failure in colorectal cancer (CRC), and it is associated with the deregulation of certain microRNAs. In this study, we constructed the microRNA-mRNA network consisting of 2275 microRNAs and 7045 target genes, collected the known microRNAs related to CRC-r...


    pub_type: 杂志文章


    authors: Liao F,Chen X,Peng P,Dong W

    更新日期:2020-10-08 00:00:00

  • Skin aging: are adipocytes the next target?

    abstract::Dermal white adipose tissue (dWAT) is increasingly appreciated as a special fat depot. The adipocytes in this depot exert a variety of unique effects on their surrounding cells and can undergo massive phenotypic changes. Significant modulation of dWAT content can be observed both in intrinsically and extrinsically age...


    pub_type: 杂志文章,评审


    authors: Kruglikov IL,Scherer PE

    更新日期:2016-07-01 00:00:00

  • Leukocyte telomere length is inversely associated with arterial wave reflection in 566 normotensive and never-treated hypertensive subjects.

    abstract::Telomeres are short segments in chromosome ends, the length of which is reduced during cell lifecycles. We examined the association of mean leukocyte telomere length (LTL) and short telomere proportion (STP) with hemodynamic variables in normotensive and never-treated hypertensive volunteers (n=566, 19-72 years). STP ...


    pub_type: 杂志文章


    authors: Honkonen M,Vääräniemi K,Saijonmaa O,Nyman A,Tikkakoski AJ,Koskela J,Lehtimäki T,Kähönen M,Mustonen J,Fyhrquist F,Pörsti I

    更新日期:2020-06-23 00:00:00

  • Neuroprotective effects and mechanisms of action of nicotinamide mononucleotide (NMN) in a photoreceptor degenerative model of retinal detachment.

    abstract::Currently, no pharmacotherapy has been proven effective in treating photoreceptor degeneration in patients. Discovering readily available and safe neuroprotectants is therefore highly sought after. Here, we investigated nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD+), in a re...


    pub_type: 杂志文章


    authors: Chen X,Amorim JA,Moustafa GA,Lee JJ,Yu Z,Ishihara K,Iesato Y,Barbisan P,Ueta T,Togka KA,Lu L,Sinclair DA,Vavvas DG

    更新日期:2020-12-29 00:00:00

  • Clinical burden of autosomal dominant polycystic kidney disease.

    abstract::There are no specific therapies for autosomal dominant polycystic kidney disease (ADPKD), and clinical data evaluating the effects of non-specific therapies on ADPKD patients are scarce. We therefore evaluated those effects using data from a longitudinal health insurance database collected from 2000-2010. We individua...


    pub_type: 杂志文章


    authors: Hung PH,Lin CH,Hung KY,Muo CH,Chung MC,Chang CH,Chung CJ

    更新日期:2020-02-24 00:00:00

  • The mechanism of submandibular gland dysfunction after menopause may be associated with the ferroptosis.

    abstract::Salivary gland dysfunction is a common symptom that occurs after menopause. This study was performed to investigate the mechanism of salivary gland dysfunction to confirm the relationship between ferroptosis and salivary gland dysfunction by ovariectomy. Forty-eight female rats were randomly divided into four groups (...


    pub_type: 杂志文章


    authors: Kwon HK,Kim JM,Shin SC,Sung ES,Kim HS,Park GC,Cheon YI,Lee JC,Lee BJ

    更新日期:2020-11-05 00:00:00

  • Quantitative proteomics of rat livers shows that unrestricted feeding is stressful for proteostasis with implications on life span.

    abstract::Studies in young mammals on the molecular effects of food restriction leading to prolong adult life are scares. Here, we used high-throughput quantitative proteomic analysis of whole rat livers to address the molecular basis for growth arrest and the apparent life-prolonging phenotype of the food restriction regimen. ...


    pub_type: 杂志文章


    authors: Gat-Yablonski G,Finka A,Pinto G,Quadroni M,Shtaif B,Goloubinoff P

    更新日期:2016-08-01 00:00:00

  • Hypoxia induces pulmonary artery smooth muscle dysfunction through mitochondrial fragmentation-mediated endoplasmic reticulum stress.

    abstract::Pulmonary arterial hypertension (PAH) is characterized by pulmonary artery smooth muscle cell (PASMC) dysfunction. However, the underlying mechanisms of PASMC dysfunction remain largely unknown. Here, we show that mitochondrial fragmentation contributes to PASMC dysfunction through enhancement of endoplasmic reticulum...


    pub_type: 杂志文章


    authors: Zhuan B,Wang X,Wang MD,Li ZC,Yuan Q,Xie J,Yang Z

    更新日期:2020-11-18 00:00:00