Effect of grape seed extract (GSE) on functional activity and mineralization of OD-21 and MDPC-23 cell lines.

Abstract:

:Recent studies on functional tissue regeneration have focused on substances that favor cell proliferation and differentiation, including the bioactive phenolic compounds present in grape seed extract (GSE). The aim of this investigation was to evaluate the stimulatory potential of GSE in the functional activity of undifferentiated pulp cells and odontoblast-like cells. OD-21 and MDPC-23 cell lines were cultivated in odontogenic medium until subconfluence, seeded in 24-well culture plates in a concentration of 2x104/well and divided into: 1) OD-21 without GSE; 2) OD-21+10 µg/mL of GSE; 3) MDPC-23 without GSE; 4) MDPC-23+10 µg/mL of GSE. Cell proliferation, in situ detection of alkaline phosphatase (ALP) and total protein content were assessed after 3, 7 and 10 days, and mineralization was evaluated after 14 days. The data were analyzed by ANOVA statistical tests set at a 5% level of significance. Results revealed that cell proliferation increased after 10 days, and protein content, after 7 days of culture in MDPC-23 cells. In situ ALP staining intensity was higher in undifferentiated pulp cells and odontoblast-like cells after 7 and 10 days, respectively. A discrete increase in MDPC-23 mineralization after GSE treatment was observed despite OD-21 cells presenting a decrease in mineralized nodule deposits. Data suggest that GSE favors functional activity of differentiated cells more broadly than undifferentiated cells (OD-21). More studies with different concentrations of GSE must be conducted to confirm its benefits to cells regarding dentin regeneration.

journal_name

Braz Oral Res

journal_title

Brazilian oral research

authors

Coelho MC,Sanchez PKV,Fernandes RR,Souza FPP,Siéssere S,Bombonato-Prado KF

doi

10.1590/1807-3107bor-2019.vol33.0013

subject

Has Abstract

pub_date

2019-02-11 00:00:00

pages

e013

eissn

1806-8324

issn

1807-3107

pii

S1806-83242019000100207

journal_volume

33

pub_type

杂志文章
  • Low prevalence of gingival overgrowth associated to new imunossupressive protocols with cyclosporin.

    abstract::Gingival overgrowth (GO) is a frequent finding in patients treated with cyclosporine (CsA). This study investigated the prevalence and severity of GO in patients who received kidney transplant and CsA therapy, as well as associations with pharmacological and clinical factors. This cross-sectional study included 63 kid...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242011005000016

    authors: Wentz LA,Oliveira SC,Moreira CH,Rösing CK

    更新日期:2012-01-01 00:00:00

  • Cytologic alterations in the oral mucosa after chronic exposure to ethanol.

    abstract::The effects of ethanol alone on the oral mucosa are still poorly understood, especially because there are few non-smoking chronic consumers of alcoholic beverages. The aim of this study was to evaluate the frequency of micronucleus, abnormal nucleus/cytoplasm ratio, pyknosis, karyorrhexis and karyolysis in exfoliated ...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242006000200002

    authors: Reis SR,do Espírito Santo AR,Andrade MG,Sadigursky M

    更新日期:2006-04-01 00:00:00

  • Electric toothbrush for biofilm control in individuals with Down syndrome: a crossover randomized clinical trial.

    abstract::Poor oral hygiene seems to be the norm in children and teenagers with Down Syndrome (DS). Advances in design and types of toothbrushes may improve biofilm control. This randomized, single-blind, crossover clinical trial evaluated the effectiveness of electric toothbrushes regarding mechanical control of biofilm in chi...

    journal_title:Brazilian oral research

    pub_type: 杂志文章,随机对照试验

    doi:10.1590/1807-3107bor-2020.vol34.0057

    authors: Silva AM,Miranda LFB,AraÚjo ASM,Prado JÚnior RR,Mendes RF

    更新日期:2020-06-19 00:00:00

  • New formula to objectively evaluate skeletal maturation using lateral cephalometric radiographs.

    abstract::The aim of this study was to establish two new formulas for objectively evaluating skeletal maturation of cervical vertebrae in female and male Brazilian subjects using lateral cephalometric radiographs. The sample included 128 girls and 110 boys, aged 7.0 to 15.9 years, from the files of the Oral Radiology Clinic, Pi...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242007000400009

    authors: Caldas Mde P,Ambrosano GM,Haiter Neto F

    更新日期:2007-10-01 00:00:00

  • Effect of occlusal splint thickness on electrical masticatory muscle activity during rest and clenching.

    abstract::The extent of separation between the maxillary and mandibular teeth in the fabrication of interocclusal splints designed to achieve efficiency and muscle relaxation is controversial and undefined in the literature. Based on this premise, the aim of this study was to evaluate the effect of interocclusal splint thicknes...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242011000600006

    authors: Pita MS,Ribeiro AB,Garcia AR,Pedrazzi V,Zuim PR

    更新日期:2011-11-01 00:00:00

  • Histological analysis of biocompatibility of ionomer cements with an acid-base reaction.

    abstract::The purpose of this study was to evaluate the inflammatory and cure events of acid-based reactions using glass ionomer cement used for cementation of crowns, bridges, onlays and orthodontic bands implanted in subcutaneous tissue, at different time intervals. A total of 48 male Wistar rats were used, distributed into 4...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/S1806-83242014.50000003

    authors: Santos RL,Moura Mde F,Carvalho FG,Guênes GM,Alves PM,Pithon MM

    更新日期:2014-01-01 00:00:00

  • Malocclusion and deleterious oral habits among adolescents in a developing area in northeastern Brazil.

    abstract::Although malocclusions represent a serious public health issue, there is insufficient information about this problem in adolescents in Brazil, especially in poorer areas. The purpose of this cross-sectional study was to estimate the prevalence of facial alterations, dental malocclusions, and deleterious oral habits (D...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242012005000027

    authors: Thomaz EB,Cangussu MC,Assis AM

    更新日期:2013-01-01 00:00:00

  • Effects of temporal muscle detachment and coronoidotomy on facial growth in young rats.

    abstract::This study analyzed the effects of unilateral detachment of the temporal muscle and coronoidotomy on facial growth in young rats. Thirty one-month-old Wistar rats were distributed into three groups: detachment, coronoidotomy and sham-operated. Under general anesthesia, unilateral detachment of the temporal muscle was ...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242012000400011

    authors: Gomes FE,Moraes RB,Luz JG

    更新日期:2012-07-01 00:00:00

  • Anti-dentine Salivary SIgA in young adults with a history of dental trauma in deciduous teeth.

    abstract::Anti-dentin autoantibodies are associated with inflammatory root resorption in permanent teeth and are modulated by dental trauma and orthodontic force. However, it is not known whether deciduous tooth trauma can stimulate the development of a humoral immune response against dentin. The aim of this study was to evalua...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2015.vol29.0102

    authors: Seixas GF,Garbelini CC,Aida KL,Cheirubim AP,Venâncio EJ,Ramos Sde P

    更新日期:2015-01-01 00:00:00

  • Analysis of mesiodistal angulations of preadjusted brackets.

    abstract::Manufacturers offer various prescriptions of preadjusted brackets for use in the "straight-wire" orthodontic technique. However, the need to incorporate bends in the rectangular wires during orthodontic finishing has led to concerns regarding the type of prescription chosen and the credibility of information provided ...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2014.vol28.0038

    authors: Mendonça MR,Verri AC,Fabre AF,Cuoghi OA

    更新日期:2014-01-01 00:00:00

  • Social disparity and oral health.

    abstract::There is a clear reported association between social disparity and oral health, for example, between dental caries and malnutrition in children. This fact is detected in several studies, and also found amongst the Brazilian population. However, several efforts have been made to improve the quality of life of the popul...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242012000700004

    authors: Navarro MF,Modena KC,Bresciani E

    更新日期:2012-01-01 00:00:00

  • Microtensile bond strength of CAD/CAM materials to dentin under different adhesive strategies.

    abstract::The present study aimed to evaluate the microtensile bond strength (μTBS) of novel computer-aided design/computer-aided manufacturing (CAD/CAM) restorative materials to dentin using different adhesive strategies. Thirty-two crowns were milled using CAD/CAM materials (Vita Mark II as control, Vita Suprinity, Vita Enami...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2017.vol31.0109

    authors: Bellan MC,Cunha PFJSD,Tavares JG,Spohr AM,Mota EG

    更新日期:2017-12-18 00:00:00

  • Use of chewing gum containing 15% of xylitol and reduction in mutans streptococci salivary levels.

    abstract::Frequent use of Xylitol may decrease the S. mutans levels. However, very little is known about whether this effect on the levels of cariogenic bacteria is maintained after the interruption of short-term usage of xylitol. This study aimed at evaluating changes in mutans streptococci (MS) salivary levels after using a c...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242010000200003

    authors: Fraga CP,Mayer MP,Rodrigues CR

    更新日期:2010-04-01 00:00:00

  • Effect of filling technique on the bond strength of methacrylate and silorane-based composite restorations.

    abstract::The bond strength of methacrylate (Z350, 3M ESPE) and silorane (P90, 3M ESPE) restorations, using different cavity filling techniques, was investigated. Cavities (6 × 3 × 3) in bovine teeth were filled using bulk, oblique, or horizontal increments. A push-out test was carried out after 24 h. Data were statistically an...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2016.vol30.0044

    authors: Machado FW,Borges FB,Cenci MS,Moraes RR,Boscato N

    更新日期:2016-01-01 00:00:00

  • Evaluation of surface characteristics of Ti-6Al-4V and Tilite alloys used for implant abutments.

    abstract::The aim of this study was to evaluate surface free energy (SFE), surface roughness (SR) and surface hardness (SH) of two commercially available materials for fabricating dental implant abutments. In addition, the specimens were investigated by scanning electron microscopy (SEM) to determine the surface morphology. Twe...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242006000400005

    authors: Lima EM,Silva WJ,Moura JS,Faot F,Del Bel Cury AA

    更新日期:2006-10-01 00:00:00

  • Evaluation of the roughness and mass loss of the flowable composites after simulated toothbrushing abrasion.

    abstract::The purpose of this study was to measure mass loss and surface roughness changes of different brands of flowable resin composites after a simulated toothbrushing test. The null hypotheses were that there would be no differences in mass loss and no significant changes in surface roughness after this test and that there...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242004000200012

    authors: Garcia FC,Wang L,D'Alpino PH,Souza JB,Araújo PA,Mondelli RF

    更新日期:2004-04-01 00:00:00

  • Evaluation of the Image-Pro Plus 4.5 software for automatic counting of labeled nuclei by PCNA immunohistochemistry.

    abstract::The objective of this study was to create and evaluate a routine (macro) using Image-Pro Plus 4.5 software (Media Cybernetics, Silver Spring, USA) for automatic counting of labeled nuclei by proliferating cell nuclear antigen (PCNA) immunohistochemistry. A total of 154 digital color images were obtained from eleven se...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242004000200002

    authors: Francisco JS,Moraes HP,Dias EP

    更新日期:2004-04-01 00:00:00

  • Administrative and clinical denials by a large dental insurance provider.

    abstract::The objective of this study was to assess the prevalence and the type of claim denials (administrative, clinical or both) made by a large dental insurance plan. This was a cross-sectional, observational study, which retrospectively collected data from the claims and denial reports of a dental insurance company. The sa...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2015.vol29.0041

    authors: Miranda GE,Siqueira MC,Ferreira RL,Delwing F,Daruge Júnior E

    更新日期:2015-01-01 00:00:00

  • Risk factors associated with the failure of miniscrews - A ten-year cross sectional study.

    abstract::The aims of this study were to identify (1) patient-related factors (sex, age, craniofacial pattern and smoking habit), (2) miniscrews implants (MSI)-related factors (length and diameter) and (3) location-related factors [bone (maxilla or mandible) and area (buccal, lingual and alveolar ridge)] that may be associated ...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2016.vol30.0124

    authors: Melo AC,Andrighetto AR,Hirt SD,Bongiolo AL,Silva SU,Silva MA

    更新日期:2016-10-24 00:00:00

  • Surface agents' influence on the flexural strength of bilaminated ceramics.

    abstract::The objective of this study was to evaluate the influence of different surface agents on the flexural strength of a ceramic system. Eighty bar-shaped specimens of zirconia were divided into four groups according to the agent to be used: group Control--to be cleaned with alcohol; group VM9--application of a fluid layer...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242013000400004

    authors: Lima JM,Anami LC,Rippe MP,de Melo RM,Bottino MA,Valera MC,Araújo MA

    更新日期:2013-07-01 00:00:00

  • Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas.

    abstract::The aim of this study was to determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of lower lip squamous cell carcinomas (LLSCCs) and to correlate the findings with clinicopathological parameters (tumor size/extent, regional lymph node metastasis, clinical stage, and histopathological grade ...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2016.vol30.0130

    authors: da Cunha FA Filho,Aguiar MC,Souza LB,Pinto LP,Godoy GP,Alves PM,Nonaka CF

    更新日期:2016-12-22 00:00:00

  • Clinical and biochemical evaluation of the saliva of patients with xerostomia induced by radiotherapy.

    abstract::Clinical aspects and biochemical properties in the saliva of 21 patients prior to and following radiotherapy for head and neck cancer were evaluated (experimental group) and compared with the same properties in a control group of 21 subjects free of cancer. Salivary flow was evaluated by measuring the time necessary, ...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242004000100013

    authors: de Barros Pontes C,Polizello AC,Spadaro AC

    更新日期:2004-01-01 00:00:00

  • The influence of desensitizing dentifrices on pain induced by in-office bleaching.

    abstract::The purpose of this study was to evaluate whether the use of desensitizing dentifrices used 15 days prior to and after in-office tooth bleaching could eliminate or reduce tooth sensitivity. After institutional review board approval and informed consent, 45 subjects were selected and divided into 3 groups according to ...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/S1806-83242013000600012

    authors: Thiesen CH,Rodrigues Filho R,Prates LH,Sartori N

    更新日期:2013-11-01 00:00:00

  • Morphology of the dentin on primary molars after the application of phosphoric acid under different conditions.

    abstract::The aim of this study was to evaluate changes in the superficial dentin pattern of primary teeth after applying different phosphoric acid concentrations and conditioning times. Twenty-four dentin surfaces were divided in 4 groups with 10 teeth each: GI -no treatment; GII to GIV -phosphoric acid at 37%, 32%, and 10% re...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242010000300011

    authors: Pimenta RA,Penido CV,Cruz Rde A,Alves JB

    更新日期:2010-07-01 00:00:00

  • Characteristics of masticatory muscles in children with unilateral posterior crossbite.

    abstract::The aim of this study was to detect possible differences in the EMG (electromyography) activity, chewing rate (CR), cycle duration (CD) and preferred chewing side (PCS) between children with and without unilateral posterior crossbite. Thirty-seven children aged from 7 to 10 years were selected from the clinic of the D...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242010000200013

    authors: Andrade Ada S,Gavião MB,Gameiro GH,De Rossi M

    更新日期:2010-04-01 00:00:00

  • Inhibitory activity of root canal irrigants against Candida albicans, Enterococcus faecalis and Staphylococcus aureus.

    abstract::The present study evaluated the antimicrobial activity of three root canal irrigants against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus. These microorganisms were incubated in the presence of citric acid (6 and 10%), EDTA (17%), and NaOCl (0.5, 1.0, 2.5, and 5.25%). Agar diffusion tests were pe...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242010000400006

    authors: Fidalgo TK,Barcelos R,Portela MB,Soares RM,Gleiser R,Silva-Filho FC

    更新日期:2010-10-01 00:00:00

  • Salivary stress biomarkers and anxiety symptoms in children with and without temporomandibular disorders.

    abstract::The etiology of temporomandibular disorders (TMD), which are considered as a heterogeneous group of psychophysiological disturbances, remains a controversial issue in clinical dentistry. This study aimed to evaluate whether the salivary alpha-amylase (sAA), cortisol levels, and anxiety symptoms differ between children...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2017.vol31.0078

    authors: Kobayashi FY,Gavião MBD,Marquezin MCS,Fonseca FLA,Montes ABM,Barbosa TS,Castelo PM

    更新日期:2017-09-28 00:00:00

  • Etiologic role of root canal infection in apical periodontitis and its relationship with clinical symptomatology.

    abstract::Evidence shows the polymicrobial etiology of endodontic infections, in which bacteria and their products are the main agents for the development, progression, and dissemination of apical periodontitis. Microbial factors in necrotic root canals (e.g., endotoxin) may spread into apical tissue, evoking and supporting a c...

    journal_title:Brazilian oral research

    pub_type: 杂志文章,评审

    doi:10.1590/1807-3107bor-2018.vol32.0069

    authors: Gomes BPFA,Herrera DR

    更新日期:2018-10-18 00:00:00

  • Effects of different torque levels on the implant-abutment interface in a conical internal connection.

    abstract::The fit of the implant-abutment interface was assessed by the metallographic technique and by scanning electron microscopy (SEM), using solid abutment types at different torque levels. Forty Morse taper connections and forty solid abutments were used at different torque levels (repeated after 10 minutes) in the follow...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2016.vol30.0040

    authors: Gehrke SA,Shibli JA,Aramburú Junior JS,de Val JE,Calvo-Girardo JL,Dedavid BA

    更新日期:2016-01-01 00:00:00

  • Effects of alpha-tocopherol on fracture resistance after endodontic treatment, bleaching and restoration.

    abstract::This study evaluated the effects of 10% alphatocopherol on the fracture resistance of endodontically treated teeth subjected to tooth bleaching with hydrogen peroxide and immediately restored with composite resin. Fifty bovine incisors were selected, including 10 sound teeth that constituted the control group (G1 (C))...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2016.vol30.0069

    authors: Jordão-Basso KC,Kuga MC,Dantas AA,Tonetto MR,Lima SN,Bandéca MC

    更新日期:2016-05-20 00:00:00