Abstract:
:Recent studies on functional tissue regeneration have focused on substances that favor cell proliferation and differentiation, including the bioactive phenolic compounds present in grape seed extract (GSE). The aim of this investigation was to evaluate the stimulatory potential of GSE in the functional activity of undifferentiated pulp cells and odontoblast-like cells. OD-21 and MDPC-23 cell lines were cultivated in odontogenic medium until subconfluence, seeded in 24-well culture plates in a concentration of 2x104/well and divided into: 1) OD-21 without GSE; 2) OD-21+10 µg/mL of GSE; 3) MDPC-23 without GSE; 4) MDPC-23+10 µg/mL of GSE. Cell proliferation, in situ detection of alkaline phosphatase (ALP) and total protein content were assessed after 3, 7 and 10 days, and mineralization was evaluated after 14 days. The data were analyzed by ANOVA statistical tests set at a 5% level of significance. Results revealed that cell proliferation increased after 10 days, and protein content, after 7 days of culture in MDPC-23 cells. In situ ALP staining intensity was higher in undifferentiated pulp cells and odontoblast-like cells after 7 and 10 days, respectively. A discrete increase in MDPC-23 mineralization after GSE treatment was observed despite OD-21 cells presenting a decrease in mineralized nodule deposits. Data suggest that GSE favors functional activity of differentiated cells more broadly than undifferentiated cells (OD-21). More studies with different concentrations of GSE must be conducted to confirm its benefits to cells regarding dentin regeneration.
journal_name
Braz Oral Resjournal_title
Brazilian oral researchauthors
Coelho MC,Sanchez PKV,Fernandes RR,Souza FPP,Siéssere S,Bombonato-Prado KFdoi
10.1590/1807-3107bor-2019.vol33.0013subject
Has Abstractpub_date
2019-02-11 00:00:00pages
e013eissn
1806-8324issn
1807-3107pii
S1806-83242019000100207journal_volume
33pub_type
杂志文章abstract::Based on aroeira's (Myracrodruon urundeuva) antimicrobial activity and a future trend to compose intracanal medication, the aim of this study was to assess in vivo inflamatory tissue response to the extracts by edemogenic and histological analysis containing inactivated facultative and anaerobic microorganisms. For ed...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2018.vol32.0042
更新日期:2018-05-24 00:00:00
abstract::The aim of this study was to detect possible differences in the EMG (electromyography) activity, chewing rate (CR), cycle duration (CD) and preferred chewing side (PCS) between children with and without unilateral posterior crossbite. Thirty-seven children aged from 7 to 10 years were selected from the clinic of the D...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242010000200013
更新日期:2010-04-01 00:00:00
abstract::The purpose of the present study was to evaluate the relationship between psychological variables and the clinical diagnosis of temporomandbular disorders (TMD) in 12-year-old adolescents. TMD pain was assessed by RDC/TMD examination (Research Diagnostic Criteria for Temporomandibular Disorders) (Axis I and II). Five-...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242009000200011
更新日期:2009-04-01 00:00:00
abstract::This study evaluated the effect of cleanser solutions on the color of heat-polymerized acrylic resin (HPAR) and on the brightness of dental alloys with 180 immersion trials. Disk-shaped specimens were made with I) commercially pure titanium, II) nickel-chromium-molybdenum-titanium, III) nickel-chromium molybdenum, and...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2014.vol28.0017
更新日期:2014-01-01 00:00:00
abstract::This study evaluated the effect of implantoplasty on different bone insertion levels of exposed implants. A model of the Bone Level Tapered implant (Straumann Institute, Waldenburg, Switzerland) was created through the Rhinoceros software (version 5.0 SR8, McNeel North America, Seattle, WA, USA). The abutment was fixe...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2017.vol31.0096
更新日期:2017-12-07 00:00:00
abstract::Oral complications of RA may include temporomandibular joint disorders, mucosa alterations and symptoms of dry mouth. The aim of this study was to evaluate the salivary gland function of subjects with rheumatoid arthritis (RA) comparing it to healthy controls. Subjects with other systemic conditions known to affect sa...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2016.vol30.0106
更新日期:2016-10-10 00:00:00
abstract::The objective of the study was to analyze the surface area (SA) of the wear caused by simulated chewing on human enamel and opposing restorative material, namely: composite resin (CR), porcelain fused to metal (PFM), lithium disilicate (LD), or monolithic zirconia (MZr). Forty-eight premolars were selected as enamel s...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2019.vol33.0121
更新日期:2020-01-10 00:00:00
abstract::The aim of this study was to investigate the effects of low-intensity pulsed ultrasound (LIPUS) on the osteogenic differentiation of dental follicle cells (DFCs) in vitro and on the regenerative effects of DFC-OsteoBoneTM complexes in vivo. DFCs were isolated and characterized. In the in vitro study, DFCs were culture...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2019.vol33.0045
更新日期:2019-09-09 00:00:00
abstract::The aim of this study was to evaluate the demographic, clinical, and therapeutic characteristics and predictive factors of poor prognosis in patients with primary oral squamous cell carcinoma (OSCC) in Uruguay. Medical records of patients with the diagnosis of primary OSCC treated between 2000 and 2010 in Uruguayan pu...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2015.vol29.0075
更新日期:2015-01-01 00:00:00
abstract::The purpose of this study was to evaluate the inflammatory and cure events of acid-based reactions using glass ionomer cement used for cementation of crowns, bridges, onlays and orthodontic bands implanted in subcutaneous tissue, at different time intervals. A total of 48 male Wistar rats were used, distributed into 4...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/S1806-83242014.50000003
更新日期:2014-01-01 00:00:00
abstract::The sense of coherence (SOC) is a measure of global orientation regarding the ability of individuals to cope with stressful situations. The aim of the present study was to evaluate the association between SOC and quality of life (QoL) and clinical and sociodemographic characteristics among survivors of oral, oropharyn...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2020.vol34.0009
更新日期:2020-02-07 00:00:00
abstract::Sickle cell anemia (SCA) is the most prevalent genetic disease worldwide. Recurrent vaso-occlusive infarcts predispose SCA patients to infections, which are the primary causes of morbidly and mortality. This study aimed to evaluate the relationship between SCA and endodontic diseases. Personal information, medical dat...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2016.vol30.0060
更新日期:2016-05-20 00:00:00
abstract::The aim of this study was to evaluate surface free energy (SFE), surface roughness (SR) and surface hardness (SH) of two commercially available materials for fabricating dental implant abutments. In addition, the specimens were investigated by scanning electron microscopy (SEM) to determine the surface morphology. Twe...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242006000400005
更新日期:2006-10-01 00:00:00
abstract::The objective of this study was to describe the outcomes of an infant oral health programme 3 years after implementation, a programme focused on health education for parents and caries prevention methods for children in a baby clinic. A retrospective cohort study was carried out. The setting was the Infant Oral Health...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242004000100003
更新日期:2004-01-01 00:00:00
abstract::The aim of this study was to establish two new formulas for objectively evaluating skeletal maturation of cervical vertebrae in female and male Brazilian subjects using lateral cephalometric radiographs. The sample included 128 girls and 110 boys, aged 7.0 to 15.9 years, from the files of the Oral Radiology Clinic, Pi...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242007000400009
更新日期:2007-10-01 00:00:00
abstract::The aim of this study was to evaluate changes in the superficial dentin pattern of primary teeth after applying different phosphoric acid concentrations and conditioning times. Twenty-four dentin surfaces were divided in 4 groups with 10 teeth each: GI -no treatment; GII to GIV -phosphoric acid at 37%, 32%, and 10% re...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242010000300011
更新日期:2010-07-01 00:00:00
abstract::The aim of this study was to determine if multiple processing (heat-pressing) of a dental ceramic influences flexural strength, hardness, and microstructure. Ninety bar-shaped specimens (15 mm × 4 mm × 2 mm) of a pressed ceramic (Vita PM9) were fabricated and randomly divided into 6 groups (n = 15) according to the fa...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2018.vol32.0106
更新日期:2018-10-25 00:00:00
abstract::We analyzed the effect of the crosshead speed of an applied load on failure load and failure mode of restored human premolars. Fifty intact, noncarious human premolars were selected. Class II mesio-occlusodistal preparations were made with a water-cooled high-speed preparation machine, and the teeth were restored with...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2016.vol30.0003
更新日期:2016-01-01 00:00:00
abstract::The aim of this study was to analyze the efficiency of experimental light-curing resin cements (ERCs) with a ternary photo-initiator system containing diphenyliodonium hexafluorphosphate (DPI) and different amines on retention of glass-fiber posts to dentin (GFP). ERCs formulations: a 1:1 mass ratio of 2,2-bis[4-(2-hy...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2018.vol32.0116
更新日期:2018-11-14 00:00:00
abstract::Oral manifestations are common findings in human immunodeficiency virus (HIV) infected patients and frequently influence the overall health. Oral hairy leukoplakia (OHL) is strongly associated with HIV infection demonstrating its relationship with the individual's immune status and progression of immunosuppression. Th...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107BOR-2016.vol30.0118
更新日期:2016-11-28 00:00:00
abstract::The purpose of this study was to analyze luxation injuries in children between the ages of 0 and 5 years treated at an emergency service department. A total of 1,703 records, corresponding to a period of 10 years at the Emergency Center of the Baby Clinic at Londrina State University, Brazil, were analyzed. The age, g...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242011000200009
更新日期:2011-03-01 00:00:00
abstract::Evidence shows the polymicrobial etiology of endodontic infections, in which bacteria and their products are the main agents for the development, progression, and dissemination of apical periodontitis. Microbial factors in necrotic root canals (e.g., endotoxin) may spread into apical tissue, evoking and supporting a c...
journal_title:Brazilian oral research
pub_type: 杂志文章,评审
doi:10.1590/1807-3107bor-2018.vol32.0069
更新日期:2018-10-18 00:00:00
abstract::This prospective observational study sought to investigate the incidence of intraoperative pain (IOP) among emergency endodontic patients and to construct an IOP prediction model that includes preoperative pain level (PPL). All patients who underwent emergency endodontic treatment at Gazi University, Ankara, Turkey, d...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/1807-3107bor-2018.vol32.0038
更新日期:2018-08-06 00:00:00
abstract::The purpose of this study was to evaluate whether the use of desensitizing dentifrices used 15 days prior to and after in-office tooth bleaching could eliminate or reduce tooth sensitivity. After institutional review board approval and informed consent, 45 subjects were selected and divided into 3 groups according to ...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/S1806-83242013000600012
更新日期:2013-11-01 00:00:00
abstract::The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and ...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242012000500011
更新日期:2012-09-01 00:00:00
abstract::Frequent use of Xylitol may decrease the S. mutans levels. However, very little is known about whether this effect on the levels of cariogenic bacteria is maintained after the interruption of short-term usage of xylitol. This study aimed at evaluating changes in mutans streptococci (MS) salivary levels after using a c...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242010000200003
更新日期:2010-04-01 00:00:00
abstract::The objective of this study was to evaluate children's respiratory patterns in the mixed dentition, by means of acoustic rhinometry, and its relation to the upper arch width development. Fifty patients were examined, 25 females and 25 males with mean age of eight years and seven months. All of them were submitted to a...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242009000400012
更新日期:2009-10-01 00:00:00
abstract::The aim of the study was to test whether the filler composition of resin composites influences their flexural strength and modulus of elasticity. Flexural strength and modulus of elasticity were obtained through a three-point bending test. Twelve bar shaped specimens of 5 commercially available composites--Supreme (3M...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242007000100003
更新日期:2007-01-01 00:00:00
abstract::The use of acid etchants to produce surface demineralization and collagen network exposure, allowing adhesive monomers interdiffusion and consequently the formation of a hybrid layer, has been considered the most efficient mechanism of dentin bonding. The aim of this study was to compare the tensile bond strength to d...
journal_title:Brazilian oral research
pub_type: 杂志文章
doi:10.1590/s1806-83242007000100014
更新日期:2007-01-01 00:00:00
abstract::The purpose of this double-blind, randomized, crossover in situ study is to compare remineralization of preformed enamel lesions by casein phosphopeptide-stabilized amorphous calcium phosphate (CPP-ACP) and fluoride dentifrice products. During each of four 10-day experimental legs, 10 participants wore intraoral remov...
journal_title:Brazilian oral research
pub_type: 杂志文章,随机对照试验
doi:10.1590/1807-3107bor-2020.vol34.0061
更新日期:2020-06-24 00:00:00