Correlation Between C-MYC, BCL-2, and BCL-6 Protein Expression and Gene Translocation as Biomarkers in Diagnosis and Prognosis of Diffuse Large B-cell Lymphoma.

Abstract:

:This study investigates the protein expression of C-MYC, BCL-2, and BCL-6 in diffuse large B-cell lymphoma (DLBCL) and their relationship with genetic abnormalities. A retrospective study of 42 cases on paraffin-embedded tissue specimens diagnosed with DLBCL was performed using immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). The expression of C-MYC, BCL-2, BCL-6 protein, and gene abnormalities in these tissue samples was analyzed. The relationship in genetic abnormalities and Ki-67, Hans classification, gender, and age was also evaluated. It was found that the positive rate of C-MYC expression was 47.6% (20/42), the rate of C-MYC gene abnormality was 26.2% (11/42), in which gene translocation accounted for 23.8% (10/42) and gene amplification 2.4% (1/42); C-MYC protein expression was positively correlated with C-MYC gene translocation (χ2 = 11.813; P = 0.001); C-MYC gene translocation was mainly found in germinal center B cell type (χ2 = 4.029; P = 0.045). The positive rate of BCL-2 protein expression was 85.71% (36/42), the positive rate of translocation was 42.86% (18/42) and the amplification rate was 26.19% (11/42); the overexpression of BCL-2 protein was correlated with the BCL-2 translocation (χ2 = 3.407; P = 0.029). The positive rate of BCL-6 protein expression was 45.24% (19/42), the positive rate of BCL-6 translocation was 14.29% (6/42) and the positive rate of BCL-6 amplification was 7.14% (3/42); the overexpression of BCL-6 protein was significantly correlated with BCL-6 translocation (χ2 = 6.091; P = 0.014). The Ki-67 index was significantly higher in C-MYC translocation cases than in non-C-MYC translocation cases (χ2 = 4.492; P = 0.034). Taken together, our results suggest that the protein expression of C-MYC, BCL-2, and BCL-6 are positively correlated with their gene translocation. Overexpression of C-MYC, BCL-2, BCL-6 protein suggests the possibility of translocation. Therefore, immunohistochemical detection of C-MYC, BCL-2, and BCL-6 are useful in diagnosis and prognosis of DLBCL.

journal_name

Front Pharmacol

authors

Zhang Y,Wang H,Ren C,Yu H,Fang W,Zhang N,Gao S,Hou Q

doi

10.3389/fphar.2018.01497

subject

Has Abstract

pub_date

2019-01-07 00:00:00

pages

1497

issn

1663-9812

journal_volume

9

pub_type

杂志文章
  • Cellular Cholesterol Distribution Influences Proteolytic Release of the LRP-1 Ectodomain.

    abstract::Low-density lipoprotein receptor-related protein-1 (LRP-1) is a multifunctional matricellular receptor composed of a large ligand-binding subunit (515-kDa α-chain) associated with a short trans-membrane subunit (85-kDa β-chain). LRP-1, which exhibits both endocytosis and cell signaling properties, plays a key role in ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2016.00025

    authors: Dekky B,Wahart A,Sartelet H,Féré M,Angiboust JF,Dedieu S,Piot O,Devy J,Emonard H

    更新日期:2016-02-12 00:00:00

  • Tnfrsf12a-Mediated Atherosclerosis Signaling and Inflammatory Response as a Common Protection Mechanism of Shuxuening Injection Against Both Myocardial and Cerebral Ischemia-Reperfusion Injuries.

    abstract::Shuxuening injection (SXNI) is a widely prescribed herbal medicine of Ginkgo biloba extract (EGB) for cerebral and cardiovascular diseases in China. However, its curative effects on ischemic stroke and heart diseases and the underlying mechanisms remain unknown. Taking an integrated approach of RNA-seq and network pha...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2018.00312

    authors: Lyu M,Cui Y,Zhao T,Ning Z,Ren J,Jin X,Fan G,Zhu Y

    更新日期:2018-04-06 00:00:00

  • Semen cassiae Extract Inhibits Contraction of Airway Smooth Muscle.

    abstract::β2-adrenoceptor agonists are commonly used as bronchodilators to treat obstructive lung diseases such as asthma and chronic obstructive pulmonary disease (COPD), however, they induce severe side effects. Therefore, developing new bronchodilators is essential. Herbal plants were extracted and the extracts' effect on ai...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2018.01389

    authors: She YS,Ma LQ,Liu BB,Zhang WJ,Qiu JY,Chen YY,Li MY,Xue L,Luo X,Wang Q,Xu H,Zang DA,Zhao XX,Cao L,Shen J,Peng YB,Zhao P,Yu MF,Chen W,Nie X,Shen C,Chen S,Chen S,Qin G,Dai J,Chen J,Liu QH

    更新日期:2018-12-04 00:00:00

  • Fusogenic Liposomes Increase the Antimicrobial Activity of Vancomycin Against Staphylococcus aureus Biofilm.

    abstract::Objective: The aim of the present study was to encapsulate vancomycin in different liposomal formulations and compare the in vitro antimicrobial activity against Staphylococcus aureus biofilms. Methods: Large unilamellar vesicles of conventional (LUV VAN), fusogenic (LUVfuso VAN), and cationic (LUVcat VAN) liposomes e...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2019.01401

    authors: Scriboni AB,Couto VM,Ribeiro LNM,Freires IA,Groppo FC,de Paula E,Franz-Montan M,Cogo-Müller K

    更新日期:2019-11-29 00:00:00

  • To Inhibit or Enhance? Is There a Benefit to Positive Allosteric Modulation of P2X Receptors?

    abstract::The family of ligand-gated ion channels known as P2X receptors were discovered several decades ago. Since the cloning of the seven P2X receptors (P2X1-P2X7), a huge research effort has elucidated their roles in regulating a range of physiological and pathophysiological processes. Transgenic animals have been influenti...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2020.00627

    authors: Stokes L,Bidula S,Bibič L,Allum E

    更新日期:2020-05-12 00:00:00

  • α2- and β2-Adrenoreceptor-Mediated Efficacy of the Atypical Antidepressant Agomelatine Combined With Gabapentin to Suppress Allodynia in Neuropathic Rats With Ligated Infraorbital or Sciatic Nerve.

    abstract::Previous data showed that neuropathic pain induced by mechanical lesion of peripheral nerves has specific characteristics and responds differently to alleviating drugs at cephalic versus extracephalic level. This is especially true for tricyclic antidepressants currently used for alleviating neuropathic pain in humans...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2018.00587

    authors: M'Dahoma S,Poitevin M,Dabala E,Payan H,Gabriel C,Mocaër E,Bourgoin S,Hamon M

    更新日期:2018-06-07 00:00:00

  • Hypoxia-Inducible Factor (HIF) as a Target for Novel Therapies in Rheumatoid Arthritis.

    abstract::Hypoxia is an important micro-environmental characteristic of rheumatoid arthritis (RA). Hypoxia-inducible factors (HIF) are key transcriptional factors that are highly expressed in RA synovium to regulate the adaptive responses to this hypoxic milieu. Accumulating evidence supports hypoxia and HIFs in regulating a nu...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2016.00184

    authors: Hua S,Dias TH

    更新日期:2016-06-27 00:00:00

  • Computational Analysis of Therapeutic Neuroadaptation to Chronic Antidepressant in a Model of the Monoaminergic Neurotransmitter and Stress Hormone Systems.

    abstract::The clinical practice of selective serotonin reuptake inhibitor (SSRI) augmentation relies heavily on trial-and-error. Unfortunately, the drug combinations prescribed today fail to provide relief for many depressed patients. In order to identify potentially more effective treatments, we developed a computational model...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2019.01215

    authors: Camacho MB,Vijitbenjaronk WD,Anastasio TJ

    更新日期:2019-10-25 00:00:00

  • Pharmacogenomics: what is next?

    abstract::Pharmacogenomics is moving from a candidate gene strategy to large scale approaches. This is in line with the new paradigm of linking a trait to (a) pathway(s) rather than to single genes. In addition, breakthroughs in genomics offer a non-a priori assessment of implicated genes, expanding the possibilities in pharmac...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2011.00086

    authors: di Iulio J,Rotger M

    更新日期:2012-01-13 00:00:00

  • 4-(Hydroxymethyl)catechol Extracted From Fungi in Marine Sponges Attenuates Rheumatoid Arthritis by Inhibiting PI3K/Akt/NF-κB Signaling.

    abstract::Rheumatoid arthritis (RA) is a progressive autoimmune disease specific to synovial joints; it causes joint damage and other systemic abnormalities, thereby leading to physical disability and early mortality. Marine sponge-derived fungi, Pestalotiopsis sp., secrete immunosuppressive compounds in the culture broth. In t...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2018.00726

    authors: Lee JY,Kim GJ,Choi JK,Choi YA,Jeong NH,Park PH,Choi H,Kim SH

    更新日期:2018-07-20 00:00:00

  • Identification of Epigallocatechin-3- Gallate as an Inhibitor of Phosphoglycerate Mutase 1.

    abstract::Targeting metabolic enzymes is believed to provide new therapeutic opportunities for cancer therapy. Phosphoglycerate mutase 1 (PGAM1) is a glycolytic enzyme that importantly coordinates glycolysis, pentose phosphate pathway (PPP) flux and serine biosynthesis in cancer cells and hence gains increasing interest of inhi...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2017.00325

    authors: Li X,Tang S,Wang QQ,Leung EL,Jin H,Huang Y,Liu J,Geng M,Huang M,Yuan S,Yao XJ,Ding J

    更新日期:2017-05-30 00:00:00

  • Inhibition of p38 MAPK Mitigates Lung Ischemia Reperfusion Injury by Reducing Blood-Air Barrier Hyperpermeability.

    abstract::Background: Lung ischemia reperfusion injury (LIRI) is a complex pathophysiological process activated by lung transplantation and acute lung injury. The p38 mitogen-activated protein kinase (MAPK) is involved in breakdown of the endothelial barrier during LIRI, but the mechanism is still unclear. Therefore, we investi...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2020.569251

    authors: Wang T,Liu C,Pan LH,Liu Z,Li CL,Lin JY,He Y,Xiao JY,Wu S,Qin Y,Li Z,Lin F

    更新日期:2020-12-11 00:00:00

  • The Early Awareness and Alert System in Sweden: History and Current Status.

    abstract::Introduction: Over the past decades, early awareness and alert (EAA) activities and systems have gained importance and become a key early health technology assessment (HTA) tool. While a pioneer in HTA, Sweden had no national level EAA activities until 2010. We describe the evolution and current status of the Swedish ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2017.00674

    authors: Eriksson I,Wettermark B,Persson M,Edström M,Godman B,Lindhé A,Malmström RE,Ramström H,von Euler M,Bergkvist Christensen A

    更新日期:2017-10-05 00:00:00

  • Mitochondrial Defunctionalization Supresses Tim-3-Galectin-9 Secretory Pathway in Human Colorectal Cancer Cells and Thus Can Possibly Affect Tumor Immune Escape.

    abstract::The Tim-3-galectin-9 secretory pathway is known to protect various types of cancer cells against host immune surveillance. We found that pharmacologically induced mitochondrial dysfunction leads to a reduced galectin-9 expression/exocytosis in human colorectal cancer cells and re-distribution of this protein (the effe...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2019.00342

    authors: Sakhnevych SS,Yasinska IM,Fasler-Kan E,Sumbayev VV

    更新日期:2019-04-05 00:00:00

  • Gender differences in pharmacokinetics and pharmacodynamics of methadone substitution therapy.

    abstract:UNLABELLED:Gender-related differences in the pharmacological effects of drug are an emerging topic. This review examines gender differences in both pharmacokinetic and pharmacodynamic aspects of methadone, a long-acting opioid agonist that is prescribed as a treatment for opioid dependence and the management of chronic...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2015.00122

    authors: Graziani M,Nisticò R

    更新日期:2015-06-09 00:00:00

  • Aspergillus terreus (Trichocomaceae): A Natural, Eco-Friendly Mycoinsecticide for Control of Malaria, Filariasis, Dengue Vectors and Its Toxicity Assessment Against an Aquatic Model Organism Artemia nauplii.

    abstract::Vector-borne diseases like malaria, filariasis, and dengue are transmitted by mosquitoes and they cause global mortality and morbidity due to an increased resistance against commercial insecticides. The present study was aimed to evaluate the neurobehavioral toxicity, knock-down effect, histopathology, ovicidal, adult...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2018.01355

    authors: Ragavendran C,Srinivasan R,Kim M,Natarajan D

    更新日期:2018-11-26 00:00:00

  • Monocytes, Macrophages, and Metabolic Disease in Atherosclerosis.

    abstract::Atherosclerotic cardiovascular disease (CVD) is a lipid-driven chronic inflammatory disease, in which macrophages are responsible for taking up these lipids and driving disease progression. Over the years, we and others have uncovered key pathways that regulate macrophage number/function and identified how metabolic d...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2019.00666

    authors: Flynn MC,Pernes G,Lee MKS,Nagareddy PR,Murphy AJ

    更新日期:2019-06-13 00:00:00

  • A new classifier-based strategy for in-silico ion-channel cardiac drug safety assessment.

    abstract::There is currently a strong interest in using high-throughput in-vitro ion-channel screening data to make predictions regarding the cardiac toxicity potential of a new compound in both animal and human studies. A recent FDA think tank encourages the use of biophysical mathematical models of cardiac myocytes for this p...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2015.00059

    authors: Mistry HB,Davies MR,Di Veroli GY

    更新日期:2015-03-24 00:00:00

  • Nrf2 as a Potential Mediator of Cardiovascular Risk in Metabolic Diseases.

    abstract::Free radicals act as secondary messengers, modulating a number of important biological processes, including gene expression, ion mobilization in transport systems, protein interactions and enzymatic functions, cell growth, cell cycle, redox homeostasis, among others. In the cardiovascular system, the physiological gen...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2019.00382

    authors: da Costa RM,Rodrigues D,Pereira CA,Silva JF,Alves JV,Lobato NS,Tostes RC

    更新日期:2019-04-12 00:00:00

  • Pricing and Reimbursement of Biosimilars in Central and Eastern European Countries.

    abstract::Objectives: The aim of this study was to review the requirements for the reimbursement of biosimilars and to compare the reimbursement status, market share, and reimbursement costs of biosimilars in selected Central and Eastern European (CEE) countries. Methods: A questionnaire-based survey was conducted between Novem...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2017.00288

    authors: Kawalec P,Stawowczyk E,Tesar T,Skoupa J,Turcu-Stiolica A,Dimitrova M,Petrova GI,Rugaja Z,Männik A,Harsanyi A,Draganic P

    更新日期:2017-06-08 00:00:00

  • The Evaluation of Geroprotective Effects of Selected Flavonoids in Drosophila melanogaster and Caenorhabditis elegans.

    abstract::Flavonoids is an intensively studied group of natural compounds with antioxidant, antineoplastic, antihyperglycemic, cardioprotective, and neuroprotective properties. The present study intends to investigate the geroprotective action of three selected flavonoids (naringin, luteolin, chrysin) in two model organisms, Ca...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2017.00884

    authors: Lashmanova E,Zemskaya N,Proshkina E,Kudryavtseva A,Volosnikova M,Marusich E,Leonov S,Zhavoronkov A,Moskalev A

    更新日期:2017-12-07 00:00:00

  • Selective Estrogen Receptor Modulators: Cannabinoid Receptor Inverse Agonists with Differential CB1 and CB2 Selectivity.

    abstract::Selective estrogen receptor modulators (SERMs) are used to treat estrogen receptor (ER)-positive breast cancer and osteoporosis. Interestingly, tamoxifen and newer classes of SERMs also exhibit cytotoxic effects in cancers devoid of ERs, indicating a non-estrogenic mechanism of action. Indicative of a potential ER-ind...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2016.00503

    authors: Franks LN,Ford BM,Prather PL

    更新日期:2016-12-22 00:00:00

  • Dual Behavior of Long-Chain Fatty Acids and Their Cyclooxygenase/Lipoxygenase Metabolites on Human Intestinal Caco-2 Cell Growth.

    abstract::Etiology of colorectal cancer (CRC) is related, at least in part, with nutritional profile and epidemiological data indicating a key role of dietary fat on CRC pathogenesis. Moreover, inflammation and eicosanoids produced from arachidonic acid might have a pivotal role in CRC development. However, the effect of specif...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2020.529976

    authors: Storniolo CE,Cabral M,Busquets MA,Martín-Venegas R,Moreno JJ

    更新日期:2020-09-04 00:00:00

  • Prognostic and Predictive Factors in Patients with Advanced Penile Cancer Receiving Salvage (2nd or Later Line) Systemic Treatment: A Retrospective, Multi-Center Study.

    abstract::Introduction and objectives: Metastatic penile squamous cell carcinoma (PSCC) is associated with dismal outcomes with median overall survival (OS) of 6-12 months in the first-line and <6 months in the salvage setting. Given the rarity of this disease, randomized trials are difficult. Prognostic risk models may assist ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2016.00487

    authors: Buonerba C,Di Lorenzo G,Pond G,Cartenì G,Scagliarini S,Rozzi A,Quevedo FJ,Dorff T,Nappi L,Lanzetta G,Pagliaro L,Eigl BJ,Naik G,Ferro M,Galdiero M,De Placido S,Sonpavde G

    更新日期:2016-12-20 00:00:00

  • The Droplet Digital PCR: A New Valid Molecular Approach for the Assessment of B-RAF V600E Mutation in Hairy Cell Leukemia.

    abstract::Hairy cell leukemia (HCL) is a chronic lymphoproliferative B-cell disorder where the B-RAF V600E mutation has been recently detected, as reported for solid neoplasias but not for other B-cell lymphomas. The digital droplet PCR (dd-PCR) is a molecular technique that, without standard references, is able to accurately q...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2016.00363

    authors: Guerrini F,Paolicchi M,Ghio F,Ciabatti E,Grassi S,Salehzadeh S,Ercolano G,Metelli MR,Del Re M,Iovino L,Petrini I,Carulli G,Cecconi N,Rousseau M,Cervetti G,Galimberti S

    更新日期:2016-10-13 00:00:00

  • Immune Checkpoint Inhibitor-Associated Cardiotoxicity: Current Understanding on Its Mechanism, Diagnosis and Management.

    abstract::Immune checkpoint inhibitors (ICIs) that target cytotoxic T lymphocyte antigen 4, programmed cell death-1, and PD-ligand 1 have revolutionized cancer treatment, achieving unprecedented efficacy in multiple malignancies. ICIs are increasingly being used in early cancer settings and in combination with various other typ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2019.01350

    authors: Zhou YW,Zhu YJ,Wang MN,Xie Y,Chen CY,Zhang T,Xia F,Ding ZY,Liu JY

    更新日期:2019-11-29 00:00:00

  • Screening and Identification of Cardioprotective Compounds From Wenxin Keli by Activity Index Approach and in vivo Zebrafish Model.

    abstract::Wenxin Keli (WXKL) is a widely used Chinese botanical drug for the treatment of arrhythmia, which is consisted of four herbs and amber. In the present study, we analyzed the chemical composition of WXKL using liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) to tentatively identify 71 comp...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2018.01288

    authors: Liu H,Chen X,Zhao X,Zhao B,Qian K,Shi Y,Baruscotti M,Wang Y

    更新日期:2018-11-13 00:00:00

  • Cornel Iridoid Glycoside Inhibits Tau Hyperphosphorylation via Regulating Cross-Talk Between GSK-3β and PP2A Signaling.

    abstract::Neurofibrillary pathology contributes to neuronal dysfunction and correlates with the clinical progression of Alzheimer's disease (AD). Tau phosphorylation is mainly regulated by a balance of glycogen synthase kinase-3β (GSK-3β) and protein phosphatase 2A (PP2A) activities. Cornel iridoid glycoside (CIG) is a main com...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2018.00682

    authors: Yang C,Li X,Gao W,Wang Q,Zhang L,Li Y,Li L,Zhang L

    更新日期:2018-06-26 00:00:00

  • The Angiotensin-(1-7)/Mas Axis Counteracts Angiotensin II-Dependent and -Independent Pro-inflammatory Signaling in Human Vascular Smooth Muscle Cells.

    abstract::Background and Aims: Targeting inflammation is nowadays considered as a challenging pharmacological strategy to prevent or delay the development of vascular diseases. Angiotensin-(1-7) is a member of the renin-angiotensin system (RAS) that binds Mas receptors and has gained growing attention in the last years as a reg...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2016.00482

    authors: Villalobos LA,San Hipólito-Luengo Á,Ramos-González M,Cercas E,Vallejo S,Romero A,Romacho T,Carraro R,Sánchez-Ferrer CF,Peiró C

    更新日期:2016-12-15 00:00:00

  • Economic Evaluation of Oral Alendronate Therapy for Osteoporosis in Chinese Postmenopausal Women: The Impact of Medication Compliance and Persistence.

    abstract::Objective: Prevalence of osteoporosis in Chinese postmenopausal women has significantly increased over the past decade and oral bisphosphonates are the most potent antiresorptive drugs. The purpose of the present research was to evaluate the cost-effectiveness of oral alendronate for individuals with osteoporosis. We ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2020.575893

    authors: You R,Liu Z

    更新日期:2020-11-16 00:00:00