The in vivo dsRNA Cleavage Has Sequence Preference in Insects.


:Exogenous dsRNA enters the insect body and can induce the RNAi effect only when it is cleaved into siRNA. However, what kinds of base composition are easier to cut and what kinds of siRNA will be produced in vivo is largely unknown. In this study, we found that dsRNA processing into siRNA has sequence preference and regularity in insects. We injected 0.04 mg/g dsRNA into Asian corn borers or cotton bollworms according to their body weight, and then the siRNAs produced in vivo were analyzed by RNA-Seq. We discovered that a large number of siRNAs were produced with GGU nucleotide residues at the 5'- and 3'-ends and produced a siRNA peak on the sequence. Once the GGU site is mutated, the number of siRNAs will decrease significantly and the siRNA peak will also lost. However, in the red flour beetle, a member of Coleoptera, dsRNA was cut at more diverse sites, such as AAG, GUG, and GUU; more importantly, these enzyme restriction sites have a high conservation base of A/U. Our discovery regarding dsRNA in vivo cleavage preference and regularity will help us understand the RNAi mechanism and its application.


Front Physiol


Frontiers in physiology


Guan R,Hu S,Li H,Shi Z,Miao X




Has Abstract


2018-12-10 00:00:00








  • Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing.

    abstract::The rice-fish mode, a mode of ecological aquaculture, has become a popular research topic in recent years. The antioxidant capacity of fish can be affected by the type of diet. Three groups of adult common carp (initial weight 517.8 ± 50 g) were fed earthworm (group A), earthworm + duckweed (group M), and duckweed (gr...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Yang S,Luo J,Long Y,Du J,Xu G,Zhao L,Du Z,Luo W,Wang Y,He Z

    更新日期:2019-05-29 00:00:00

  • Dynamical Landscape of Heart Rhythm in Long-Term Heart Transplant Recipients: A Way to Discern Erratic Rhythms.

    abstract::It is commonly believed that higher values of heart rate variability (HRV) indices account for better organization of the network of feedback reflexes driving an organism's response to actual bodily needs. In order to evaluate this organization in heart transplant (HTX) recipients, 58 nocturnal Holter signals of 14 HT...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Wdowczyk J,Makowiec D,Gruchała M,Wejer D,Struzik ZR

    更新日期:2018-04-09 00:00:00

  • Low Nitric Oxide Bioavailability Increases Renin Production in the Collecting Duct.

    abstract::In the kidney, the stimulation of renin production by the collecting duct (CD-renin) contributes to the development of hypertension. The CD is a major nephron segment for the synthesis of nitric oxide (NO), and low NO bioavailability in the renal medulla is associated with hypertension. However, it is unknown whether ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Curnow AC,Gonsalez SR,Gogulamudi VR,Visniauskas B,Simon EE,Gonzalez AA,Majid DSA,Lara LS,Prieto MC

    更新日期:2020-11-17 00:00:00

  • Influence of Magnitude and Duration of Altered Gravity and Readaptation to 1 g on the Structure and Function of the Utricle in Toadfish, Opsanus tau.

    abstract::Gravity has remained constant during animal evolution and the neural sensory systems detecting acceleration forces have remained remarkably conserved among vertebrates. The utricular organ senses the sum of inertial force due to head translation and head tilt relative to gravitational vertical. Change in gravitational...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Boyle R,Popova Y,Varelas J

    更新日期:2018-10-22 00:00:00

  • A Comprehensive Engineering Analysis of Left Heart Dynamics After MitraClip in a Functional Mitral Regurgitation Patient.

    abstract::Percutaneous edge-to-edge mitral valve (MV) repair using MitraClip has been recently established as a treatment option for patients with heart failure and functional mitral regurgitation (MR), which significantly expands the number of patients that can be treated with this device. This study aimed to quantify the morp...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Caballero A,Mao W,McKay R,Hahn RT,Sun W

    更新日期:2020-05-07 00:00:00

  • Comprehensive Uncertainty Quantification and Sensitivity Analysis for Cardiac Action Potential Models.

    abstract::Recent efforts to ensure the reliability of computational model-based predictions in healthcare, such as the ASME V&V40 Standard, emphasize the importance of uncertainty quantification (UQ) and sensitivity analysis (SA) when evaluating computational models. UQ involves empirically determining the uncertainty in model ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Pathmanathan P,Cordeiro JM,Gray RA

    更新日期:2019-06-26 00:00:00

  • Cross-sectional and longitudinal assessment of arterial stiffening with age in European and chinese populations.

    abstract::As arteries become stiffer with aging, reflected waves move faster and augment late systolic pressure. Few studies have described the age-related changes in both peripheral and central systolic blood pressures in populations. We investigated the age dependency of peripheral (pSBP) and central (cSBP) systolic pressure ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Wojciechowska W,Li Y,Stolarz-Skrzypek K,Kawecka-Jaszcz K,Staessen JA,Wang JG,European Project on Genes in Hypertension and the JingNing Study Investigators.

    更新日期:2012-06-15 00:00:00

  • Modulation of Ion Channels in the Superior Cervical Ganglion Neurons by Myocardial Ischemia and Fluvastatin Treatment.

    abstract::Background: The superior cervical ganglion (SCG) of the autonomic nervous system plays an important role in different cardiovascular diseases. In this study, we investigated the effects of ischemia and fluvastatin treatment on the ion channel characteristics of SCG neurons in a rabbit myocardial ischemia (MI) model. M...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Cheng L,Wang X,Liu T,Tse G,Fu H,Li G

    更新日期:2018-09-10 00:00:00

  • P2Y2 receptor knock-out mice display normal NaCl absorption in medullary thick ascending limb.

    abstract::Local purinergic signals modulate renal tubular transport. Acute activation of renal epithelial P2 receptors causes inhibition of epithelial transport and thus, should favor increased water and salt excretion by the kidney. So far only a few studies have addressed the effects of extracellular nucleotides on ion transp...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Marques RD,Praetorius HA,Leipziger J

    更新日期:2013-10-09 00:00:00

  • Abnormal Haemodynamic Flow Patterns in Bicuspid Pulmonary Valve Disease.

    abstract::Abnormal flow patterns in the aortas of those with bicuspid aortic valves (BAVs) are increasingly recognized as important in the pathogenesis of aortic dilatation but pulmonary flow patterns in bicuspid pulmonary valves have not been studied. Bicuspid pulmonary valve disease is rare and a small numbers of case reports...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Bissell MM,Loudon M,Neubauer S,Myerson SG

    更新日期:2017-05-31 00:00:00

  • Positive Impact of Thermal Manipulation During Embryogenesis on Foie Gras Production in Mule Ducks.

    abstract::Animal studies have shown that very early life events may have programing effects on adult metabolism and health. In this study, we aim, for the first, time to elucidate the effects of embryonic thermal manipulation (TM) on the performance of overfed mule ducks, in particular for the production of foie gras (fatty liv...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Massimino W,Davail S,Bernadet MD,Pioche T,Tavernier A,Ricaud K,Gontier K,Bonnefont C,Manse H,Morisson M,Fauconneau B,Collin A,Panserat S,Houssier M

    更新日期:2019-12-12 00:00:00

  • Stars and stripes in pancreatic cancer: role of stellate cells and stroma in cancer progression.

    abstract::Pancreatic cancer is a devastating disease with an unacceptably high mortality to incidence ratio. Traditional therapeutic approaches such as surgery in combination with chemo- or radiotherapy have had limited efficacy in improving the outcome of this disease. Up until just under a decade ago, the prominent desmoplast...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审


    authors: Wilson JS,Pirola RC,Apte MV

    更新日期:2014-02-14 00:00:00

  • Behavioral, Morphological, and Gene Expression Changes Induced by 60Co-γ Ray Irradiation in Bactrocera tau (Walker).

    abstract::The sterile insect technique (SIT) may reduce pest populations by allowing sufficient amount of irradiation-induced sterile males to mate with wild females whilst maintaining mating ability comparable to wild males. Although the SIT methods are well understood, the optimal sterilizing dose and processing development s...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Cai J,Yang H,Shi S,Zhong G,Yi X

    更新日期:2018-02-20 00:00:00

  • Transport and Recovery of Gilthead Seabream (Sparus aurata L.) Sedated With Clove Oil and MS-222: Effects on Stress Axis Regulation and Intermediary Metabolism.

    abstract::Transport processes between aquaculture facilities activate the stress response in fish. To deal with these situations, the hypothalamic-pituitary-interrenal (HPI) axis releases cortisol, leading to an increase in circulating energy resources to restore homeostasis. However, if the allostatic load generated exceeds fi...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Jerez-Cepa I,Fernández-Castro M,Del Santo O'Neill TJ,Martos-Sitcha JA,Martínez-Rodríguez G,Mancera JM,Ruiz-Jarabo I

    更新日期:2019-05-31 00:00:00

  • Lifelong Effects of Thermal Challenges During Development in Birds and Mammals.

    abstract::Before they develop competent endothermy, mammals and birds are sensitive to fluctuating temperature. It follows that early life thermal environment can trigger changes to the ontogeny of thermoregulatory control. At the ecological level, we have incomplete knowledge of how such responses affect temperature tolerance ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审


    authors: Nord A,Giroud S

    更新日期:2020-05-25 00:00:00

  • An Argument for Amphetamine-Induced Hallucinations in an Invertebrate.

    abstract::Hallucinations - compelling perceptions of stimuli that aren't really there - occur in many psychiatric and neurological disorders, and are triggered by certain drugs of abuse. Despite their clinical importance, the neuronal mechanisms giving rise to hallucinations are poorly understood, in large part due to the absen...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Lee AH,Brandon CL,Wang J,Frost WN

    更新日期:2018-06-25 00:00:00

  • Integration of expression data in genome-scale metabolic network reconstructions.

    abstract::With the advent of high-throughput technologies, the field of systems biology has amassed an abundance of "omics" data, quantifying thousands of cellular components across a variety of scales, ranging from mRNA transcript levels to metabolite quantities. Methods are needed to not only integrate this omics data but to ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Blazier AS,Papin JA

    更新日期:2012-08-06 00:00:00

  • Adenosine: Direct and Indirect Actions on Gastric Acid Secretion.

    abstract::Composed by a molecule of adenine and a molecule of ribose, adenosine is a paradigm of recyclable nucleoside with a multiplicity of functions that occupies a privileged position in the metabolic and regulatory contexts. Adenosine is formed continuously in intracellular and extracellular locations of all tissues. Extra...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审


    authors: Arin RM,Gorostidi A,Navarro-Imaz H,Rueda Y,Fresnedo O,Ochoa B

    更新日期:2017-09-22 00:00:00

  • Non-linear Scaling of Passive Mechanical Properties in Fibers, Bundles, Fascicles and Whole Rabbit Muscles.

    abstract::Defining variations in skeletal muscle passive mechanical properties at different size scales ranging from single muscle fibers to whole muscles is required in order to understand passive muscle function. It is also of interest from a muscle structural point-of-view to identify the source(s) of passive tension that fu...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Ward SR,Winters TM,O'Connor SM,Lieber RL

    更新日期:2020-03-20 00:00:00

  • Partial Amino Acid Metabolism and Glutamine Synthesis as the Ammonia Defensive Strategies During Aerial Exposure in Chinese Loach Paramisgurnus dabryanus.

    abstract::The Paramisgurnus dabryanus was exposed to air to assess the changes in plasma, liver and muscle free amino acid (FAA) contents. The FAA concentrations in plasma, liver and muscle of P. dabryanus were significantly affected by aerial exposure (P < 0.05). After 12 h of aerial exposure, the plasma glutamate contents inc...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Zhang YL,Wang GY,Zhang ZH,Xie YY,Jin H,Dong ZR

    更新日期:2019-01-29 00:00:00

  • Renal erythropoietin-producing cells in health and disease.

    abstract::Erythropoietin (Epo) is an indispensable erythropoietic hormone primarily produced from renal Epo-producing cells (REPs). Epo production in REPs is tightly regulated in a hypoxia-inducible manner to maintain tissue oxygen homeostasis. Insufficient Epo production by REPs causes renal anemia and anemia associated with c...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审


    authors: Souma T,Suzuki N,Yamamoto M

    更新日期:2015-06-03 00:00:00

  • Physiological Predictors of Maximal Incremental Running Performance.

    abstract:Purpose:The aim of this study was to verify whether physiological components [vertical jumps (Squat Jump - SJ and Countermovement Jump - CMJ), eccentric utilization ratio (EUR) of vertical jumps, running economy (RE), metabolic cost (C MET ), first and second ventilatory th...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Lanferdini FJ,Silva ES,Machado E,Fischer G,Peyré-Tartaruga LA

    更新日期:2020-08-05 00:00:00

  • A Dynamic Model for Prediction of Psoriasis Management by Blue Light Irradiation.

    abstract::Clinical investigations prove that blue light irradiation reduces the severity of psoriasis vulgaris. Nevertheless, the mechanisms involved in the management of this condition remain poorly defined. Despite the encouraging results of the clinical studies, no clear guidelines are specified in the literature for the irr...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Félix Garza ZC,Liebmann J,Born M,Hilbers PA,van Riel NA

    更新日期:2017-01-26 00:00:00

  • Self-Organization of Blood Pressure Regulation: Experimental Evidence.

    abstract::Blood pressure regulation is a prime example of homeostatic regulation. However, some characteristics of the cardiovascular system better match a non-linear self-organized system than a homeostatic one. To determine whether blood pressure regulation is self-organized, we repeated the seminal demonstration of self-orga...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Fortrat JO,Levrard T,Courcinous S,Victor J

    更新日期:2016-03-31 00:00:00

  • Embryonic Exposure to Valproic Acid Affects Social Predispositions for Dynamic Cues of Animate Motion in Newly-Hatched Chicks.

    abstract::Early predispositions to preferentially orient toward cues associated with social partners have been documented in several vertebrate species including human neonates and domestic chicks. Human newborns at high familiar risk of Autism Spectrum Disorder (ASD) show differences in their attention toward these predisposed...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Lorenzi E,Pross A,Rosa-Salva O,Versace E,Sgadò P,Vallortigara G

    更新日期:2019-04-30 00:00:00

  • The Transcription Factor MafB Regulates the Susceptibility of Bactrocera dorsalis to Abamectin via GSTz2.

    abstract::Pesticide resistance is a serious problem that poses a major challenge to pest control. One of the most potent resistance mechanisms is the overexpression of genes coding for detoxification enzymes. The expression of detoxification genes is regulated by a series of transcription factors. Previous studies have revealed...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Tang GH,Xiong Y,Liu Y,Song ZH,Yang Y,Shen GM,Wang JJ,Jiang HB

    更新日期:2019-08-20 00:00:00

  • Application of Patient-Specific Computational Fluid Dynamics in Coronary and Intra-Cardiac Flow Simulations: Challenges and Opportunities.

    abstract::The emergence of new cardiac diagnostics and therapeutics of the heart has given rise to the challenging field of virtual design and testing of technologies in a patient-specific environment. Given the recent advances in medical imaging, computational power and mathematical algorithms, patient-specific cardiac models ...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章,评审


    authors: Zhong L,Zhang JM,Su B,Tan RS,Allen JC,Kassab GS

    更新日期:2018-06-26 00:00:00

  • Age and Sex Differences in Hearts of Soluble Epoxide Hydrolase Null Mice.

    abstract::Biological aging is an inevitable part of life that has intrigued individuals for millennia. The progressive decline in biological systems impacts cardiac function and increases vulnerability to stress contributing to morbidity and mortality in aged individuals. Yet, our understanding of the molecular, biochemical and...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Jamieson KL,Keshavarz-Bahaghighat H,Darwesh AM,Sosnowski DK,Seubert JM

    更新日期:2020-02-07 00:00:00

  • Exercise-Induced Hypertrophic and Oxidative Signaling Pathways and Myokine Expression in Fast Muscle of Adult Zebrafish.

    abstract::Skeletal muscle is a plastic tissue that undergoes cellular and metabolic adaptations under conditions of increased contractile activity such as exercise. Using adult zebrafish as an exercise model, we previously demonstrated that swimming training stimulates hypertrophy and vascularization of fast muscle fibers, cons...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Rovira M,Arrey G,Planas JV

    更新日期:2017-12-18 00:00:00

  • Myocardial Blood Flow and Metabolic Rate of Oxygen Measurement in the Right and Left Ventricles at Rest and During Exercise Using 15O-Labeled Compounds and PET.

    abstract::Aims: Simultaneous measurement of right (RV) and left ventricle (LV) myocardial blood flow (MBF), oxygen extraction fraction (OEF), and oxygen consumption (MVO2) non-invasively in humans would provide new possibilities to understand cardiac physiology and different patho-physiological states. Methods: We developed and...

    journal_title:Frontiers in physiology

    pub_type: 杂志文章


    authors: Kudomi N,Kalliokoski KK,Oikonen VJ,Han C,Kemppainen J,Sipilä HT,Knuuti J,Heinonen IHA

    更新日期:2019-06-19 00:00:00