DT-13 Inhibits Proliferation and Metastasis of Human Prostate Cancer Cells Through Blocking PI3K/Akt Pathway.

Abstract:

:DT-13, a saponin monomer 13 from the dwarf lilyturf tuber, was reported to exhibit anti-inflammatory, hepatoprotective, cardioprotective as well as antitumor activities in a number of tumor cells. Prostate cancer is the second leading cause of cancer death in males, discovery of novel antitumor drug for therapy of prostate cancer is expected. Aiming to evaluate whether DT-13 could become a candidate to treat prostate cancer, we recently investigated the antitumor effect of DT-13 on human prostate cancer cells and the underlying mechanism. DT-13 was found to effectively inhibit proliferation and metastasis of prostate cancer PC3 and DU145 cell lines in a dose-dependent manner. Treatment by DT-13 resulted in a mitochondria-mediated apoptosis, which was accompanied by the chromatin condensation and nuclear shrinkage in the prostate cancer cells. Moreover, DT-13 caused remarkable upregulation of Bax, Bad, Cytochrome C, cleaved -caspase 3, -caspase 9 and -PARP, in contrast to the downregulation of Bcl-2. Nevertheless, no obvious change in intracellular ROS level was observed after DT-13 treatment. We further demonstrated that DT-13 could inhibit PC3 cell metastasis in which suppression of Integrinβ1 and MMP2/9 might be involved. Western blot analysis indicated DT-13 significantly decreased the phosphorylation of PDK1, Akt, mTOR as well as p70S6K, suggesting the pro-apoptotic and anti-metastatic effects of DT-13 on prostate cancer cells might be attributed to the blockade of PI3K/Akt pathway. Collectively, our findings suggest DT-13 is worthy of further investigation as a drug candidate for the treatment of prostate cancer.

journal_name

Front Pharmacol

authors

Wang Z,Wang Y,Zhu S,Liu Y,Peng X,Zhang S,Zhang Z,Qiu Y,Jin M,Wang R,Zhong Y,Kong D

doi

10.3389/fphar.2018.01450

subject

Has Abstract

pub_date

2018-12-07 00:00:00

pages

1450

issn

1663-9812

journal_volume

9

pub_type

杂志文章
  • Circulating Tumor DNA-Based Detection of Microsatellite Instability and Response to Immunotherapy in Pancreatic Cancer.

    abstract::Pancreatic cancer is an aggressive malignancy with poor survival. Research has indicated the association of few genetic aberrations with pancreatic cancer. The data regarding the prevalence of microsatellite instability in pancreatic cancer is diverse and controversial. However, it could be an actionable target in pan...

    journal_title:Frontiers in pharmacology

    pub_type:

    doi:10.3389/fphar.2020.00023

    authors: Kamatham S,Shahjehan F,Kasi PM

    更新日期:2020-02-10 00:00:00

  • Cathepsin S Is Involved in Th17 Differentiation Through the Upregulation of IL-6 by Activating PAR-2 after Systemic Exposure to Lipopolysaccharide from Porphyromonas gingivalis.

    abstract::Positive links have been found between periodontitis and numerous diseases in humans via persistent inflammation throughout the body. However, the main factors responsible for maintaining this pro-inflammatory condition are poorly understood. The spleen, the largest secondary immune organ, is a central hub regulating ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2017.00470

    authors: Dekita M,Wu Z,Ni J,Zhang X,Liu Y,Yan X,Nakanishi H,Takahashi I

    更新日期:2017-07-17 00:00:00

  • Long-Chain Omega-3 Fatty Acids Supplementation Accelerates Nerve Regeneration and Prevents Neuropathic Pain Behavior in Mice.

    abstract::Fish oil (FO) is the main source of long chain omega-3 polyunsaturated fatty acids (ω-3 PUFAs), which display relevant analgesic and anti-inflammatory properties. Peripheral nerve injury is driven by degeneration, neuroinflammation, and neuronal plasticity which results in neuropathic pain (NP) symptoms such as allody...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2017.00723

    authors: Silva RV,Oliveira JT,Santos BLR,Dias FC,Martinez AMB,Lima CKF,Miranda ALP

    更新日期:2017-10-17 00:00:00

  • To Inhibit or Enhance? Is There a Benefit to Positive Allosteric Modulation of P2X Receptors?

    abstract::The family of ligand-gated ion channels known as P2X receptors were discovered several decades ago. Since the cloning of the seven P2X receptors (P2X1-P2X7), a huge research effort has elucidated their roles in regulating a range of physiological and pathophysiological processes. Transgenic animals have been influenti...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2020.00627

    authors: Stokes L,Bidula S,Bibič L,Allum E

    更新日期:2020-05-12 00:00:00

  • Molecular Mechanism of Action of Trimethylangelicin Derivatives as CFTR Modulators.

    abstract::The psoralen-related compound, 4,6,4'-trimethylangelicin (TMA) potentiates the cAMP/PKA-dependent activation of WT-CFTR and rescues F508del-CFTR-dependent chloride secretion in both primary and secondary airway cells homozygous for the F508del mutation. We recently demonstrated that TMA, like lumacaftor (VX-809), stab...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2018.00719

    authors: Laselva O,Marzaro G,Vaccarin C,Lampronti I,Tamanini A,Lippi G,Gambari R,Cabrini G,Bear CE,Chilin A,Dechecchi MC

    更新日期:2018-07-04 00:00:00

  • Blockade of Serotonin 5-HT2A Receptors Suppresses Behavioral Sensitization and Naloxone-Precipitated Withdrawal Symptoms in Morphine-Treated Mice.

    abstract::The increasing prescription of opioids is fueling an epidemic of addiction and overdose deaths. Morphine is a highly addictive drug characterized by a high relapse rate - even after a long period of abstinence. Serotonin (5-HT) neurotransmission participates in the development of morphine dependence, as well as the ex...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2016.00514

    authors: Pang G,Wu X,Tao X,Mao R,Liu X,Zhang YM,Li G,Stackman RW Jr,Dong L,Zhang G

    更新日期:2016-12-26 00:00:00

  • Long Non-Coding RNA in Drug Resistance of Non-Small Cell Lung Cancer: A Mini Review.

    abstract::Lung cancer is one of main causes of cancer mortality and 83% of lung cancer cases are classified as non-small cell lung cancer (NSCLC). Patients with NSCLC usually have a poor prognosis and one of the leading causes is drug resistance. With the progress of drug therapy, the emergence and development of drug resistanc...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2019.01457

    authors: Sun R,Wang R,Chang S,Li K,Sun R,Wang M,Li Z

    更新日期:2019-12-18 00:00:00

  • Validation and Psychometric Properties of the Arabic Version of the Duke Anticoagulation Satisfaction Scale (DASS).

    abstract::Background: To assess the health-related quality of life (HRQoL) of oral anticoagulant therapy users, different types of instruments are available, either general or specific tools like Duke Anticoagulation Satisfaction Scale (DASS). These tools allow the clinician to adjust the treatment regimen to focus on increasin...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2020.587489

    authors: AlAmmari M,Sultana K,AlHarbi SN,Marenga AS,AlTuraiki A,Althemery AU,Alfaifi AA,AlShehri A,Aqeel BF

    更新日期:2020-12-17 00:00:00

  • Corrigendum: Protein kinases as switches for the function of upstream stimulatory factors: implications for tissue injury and cancer.

    abstract::[This corrects the article on p. 3 in vol. 6, PMID: 25741280.]. ...

    journal_title:Frontiers in pharmacology

    pub_type: 已发布勘误

    doi:10.3389/fphar.2016.00092

    authors: Horbach T,Götz C,Kietzmann T,Dimova EY

    更新日期:2016-04-05 00:00:00

  • Prognostic and Predictive Factors in Patients with Advanced Penile Cancer Receiving Salvage (2nd or Later Line) Systemic Treatment: A Retrospective, Multi-Center Study.

    abstract::Introduction and objectives: Metastatic penile squamous cell carcinoma (PSCC) is associated with dismal outcomes with median overall survival (OS) of 6-12 months in the first-line and <6 months in the salvage setting. Given the rarity of this disease, randomized trials are difficult. Prognostic risk models may assist ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2016.00487

    authors: Buonerba C,Di Lorenzo G,Pond G,Cartenì G,Scagliarini S,Rozzi A,Quevedo FJ,Dorff T,Nappi L,Lanzetta G,Pagliaro L,Eigl BJ,Naik G,Ferro M,Galdiero M,De Placido S,Sonpavde G

    更新日期:2016-12-20 00:00:00

  • A Metabolomics Study on the Bone Protective Effects of a Lignan-Rich Fraction From Sambucus Williamsii Ramulus in Aged Rats.

    abstract::The lignan-rich fraction (SWR) of Sambucus Williamsii Ramulus, a folk herbal medicine in China for treatment of bone diseases, has previously reported to exert protective effects on bone without exerting uterotrophic effects in ovariectomized (OVX) mice. The aim of the present study was to identify the potential metab...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2018.00932

    authors: Xiao HH,Sham TT,Chan CO,Li MH,Chen X,Wu QC,Mok DK,Yao XS,Wong MS

    更新日期:2018-08-21 00:00:00

  • Administrative Data Linkage in Brazil: Potentials for Health Technology Assessment.

    abstract::Health technology assessment (HTA) is the systematic evaluation of the properties and impacts of health technologies and interventions. In this article, we presented a discussion of HTA and its evolution in Brazil, as well as a description of secondary data sources available in Brazil with potential applications to ge...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2019.00984

    authors: Ali MS,Ichihara MY,Lopes LC,Barbosa GCG,Pita R,Carreiro RP,Dos Santos DB,Ramos D,Bispo N,Raynal F,Canuto V,de Araujo Almeida B,Fiaccone RL,Barreto ME,Smeeth L,Barreto ML

    更新日期:2019-09-23 00:00:00

  • Therapeutic Anabolic and Anticatabolic Benefits of Natural Chinese Medicines for the Treatment of Osteoporosis.

    abstract::Osteoporosis is a bone disease characterized by increasing osseous fragility and fracture due to the reduced bone mass and microstructural degradation. Primary pharmacological strategies for the treatment of osteoporosis, hormone replacement treatment (HRT), and alendronate therapies may produce adverse side-effects a...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2019.01344

    authors: He J,Li X,Wang Z,Bennett S,Chen K,Xiao Z,Zhan J,Chen S,Hou Y,Chen J,Wang S,Xu J,Lin D

    更新日期:2019-11-25 00:00:00

  • Combination of Ruxolitinib and Eculizumab for Treatment of Severe SARS-CoV-2-Related Acute Respiratory Distress Syndrome: A Controlled Study.

    abstract::To date, there are no specific therapeutic strategies for treatment of COVID-19. Based on the hypothesis that complement and coagulation cascades are activated by viral infection, and might trigger an acute respiratory distress syndrome (ARDS), we report clinical outcomes of 17 consecutive cases of SARS-CoV-2-related ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2020.00857

    authors: Giudice V,Pagliano P,Vatrella A,Masullo A,Poto S,Polverino BM,Gammaldi R,Maglio A,Sellitto C,Vitale C,Serio B,Cuffa B,Borrelli A,Vecchione C,Filippelli A,Selleri C

    更新日期:2020-06-05 00:00:00

  • Altered Expression of Endoplasmic Reticulum Stress Associated Genes in Hippocampus of Learned Helpless Rats: Relevance to Depression Pathophysiology.

    abstract::The unfolded protein response (UPR) is an evolutionarily conserved defensive mechanism that is used by cells to correct misfolded proteins that accumulate in the endoplasmic reticulum. These proteins are misfolded as a result of physical stress on a cell and initiate a host of downstream effects that govern processes ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2015.00319

    authors: Timberlake MA 2nd,Dwivedi Y

    更新日期:2016-01-12 00:00:00

  • Safety, tolerability, and biomarkers of the treatment of mice with aerosolized Toll-like receptor ligands.

    abstract::We have previously discovered a synergistically therapeutic combination of two Toll-like receptor ligands, an oligodeoxynucleotide (ODN) and Pam2CSK4. Aerosolization of these ligands stimulates innate immunity within the lungs to prevent pneumonia from bacterial and viral pathogens. Here we examined the safety and tol...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2014.00008

    authors: Alfaro VY,Goldblatt DL,Valverde GR,Munsell MF,Quinton LJ,Walker AK,Dantzer R,Varadhachary A,Scott BL,Evans SE,Tuvim MJ,Dickey BF

    更新日期:2014-02-06 00:00:00

  • Fusogenic Liposomes Increase the Antimicrobial Activity of Vancomycin Against Staphylococcus aureus Biofilm.

    abstract::Objective: The aim of the present study was to encapsulate vancomycin in different liposomal formulations and compare the in vitro antimicrobial activity against Staphylococcus aureus biofilms. Methods: Large unilamellar vesicles of conventional (LUV VAN), fusogenic (LUVfuso VAN), and cationic (LUVcat VAN) liposomes e...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2019.01401

    authors: Scriboni AB,Couto VM,Ribeiro LNM,Freires IA,Groppo FC,de Paula E,Franz-Montan M,Cogo-Müller K

    更新日期:2019-11-29 00:00:00

  • MetMaxStruct: A Tversky-Similarity-Based Strategy for Analysing the (Sub)Structural Similarities of Drugs and Endogenous Metabolites.

    abstract:BACKGROUND:Previous studies compared the molecular similarity of marketed drugs and endogenous human metabolites (endogenites), using a series of fingerprint-type encodings, variously ranked and clustered using the Tanimoto (Jaccard) similarity coefficient (TS). Because this gives equal weight to all parts of the encod...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2016.00266

    authors: O'Hagan S,Kell DB

    更新日期:2016-08-22 00:00:00

  • A Mechanism of Action Study on Danggui Sini Decoction to Discover Its Therapeutic Effect on Gastric Cancer.

    abstract::Danggui Sini Decoction (DSD), a classic Chinese herb medicine (CHM) formula, has been used to treat various diseases in China for centuries. However, it remains challenging to reveal its mechanism of action through conventional pharmacological methods. Here, we first explored the mechanism of action of DSD with the as...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2020.592903

    authors: Pan B,Wang Y,Wu C,Jia J,Huang C,Fang S,Liu L

    更新日期:2021-01-11 00:00:00

  • Xuebijing Injection Alleviates Pam3CSK4-Induced Inflammatory Response and Protects Mice From Sepsis Caused by Methicillin-Resistant Staphylococcus aureus.

    abstract::A leading cause of death worldwide is sepsis that develops as a dysregulated immune response to infection. Serious infection caused by methicillin-resistant Staphylococcus aureus (MRSA) increases the difficulty of treatment in septic patients. Host-directed therapy (HDT) is an emerging approach to bacterial infections...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2020.00104

    authors: Li T,Qian Y,Miao Z,Zheng P,Shi T,Jiang X,Pan L,Qian F,Yang G,An H,Zheng Y

    更新日期:2020-02-21 00:00:00

  • A Comprehensive Strategy to Evaluate Compatible Stability of Chinese Medicine Injection and Infusion Solutions Based on Chemical Analysis and Bioactivity Assay.

    abstract::Stability of traditional Chinese medicine injection (TCMI) is an important issue related with its clinical application. TCMI is composed of multi-components, therefore, when evaluating TCMI stability, several marker compounds cannot represent global components or biological activities of TCMI. Till now, when evaluatin...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2017.00833

    authors: Li JP,Liu Y,Guo JM,Shang EX,Zhu ZH,Zhu KY,Tang YP,Zhao BC,Tang ZS,Duan JA

    更新日期:2017-11-15 00:00:00

  • The Role of Eukaryotic and Prokaryotic ABC Transporter Family in Failure of Chemotherapy.

    abstract::Over the years chemotherapy failure has been a vital research topic as researchers have been striving to discover reasons behind it. The extensive studies carried out on chemotherapeutic agents confirm that resistance to chemotherapy is a major reason for treatment failure. "Resistance to chemotherapy," however, is a ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2016.00535

    authors: El-Awady R,Saleh E,Hashim A,Soliman N,Dallah A,Elrasheed A,Elakraa G

    更新日期:2017-01-10 00:00:00

  • ErHuang Formula Improves Renal Fibrosis in Diabetic Nephropathy Rats by Inhibiting CXCL6/JAK/STAT3 Signaling Pathway.

    abstract::Diabetic nephropathy (DN) is one of the main causes of renal fibrosis and is associated with high morbidity and mortality. Traditional Chinese Medicine (TCM) therapy has a long history of usage in a clinical setting and its usage is increasing. ErHuang Formula (EHF), a Chinese herbal compound, has been clinically used...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2019.01596

    authors: Shen YL,Jiang YP,Li XQ,Wang SJ,Ma MH,Zhang CY,Zhu JY,Rahman K,Zhang LJ,Luan X,Zhang H

    更新日期:2020-01-24 00:00:00

  • Factors Associated With Response and Resistance to Cognitive Remediation in Schizophrenia: A Critical Review.

    abstract::Cognitive impairment is a central feature of schizophrenia and has shown to play a crucial role in the psychosocial function of the disorder. Over the past few years, several cognitive remediation (CR) interventions have been developed for schizophrenia, whose effectiveness has also been widely demonstrated by systema...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2018.01542

    authors: Barlati S,Deste G,Galluzzo A,Perin AP,Valsecchi P,Turrina C,Vita A

    更新日期:2019-01-10 00:00:00

  • Heme Catabolic Pathway in Inflammation and Immune Disorders.

    abstract::In recent years, the heme catabolic pathway is considered to play an important regulatory role in cell protection, apoptosis, inflammation, and other physiological and pathological processes. An appropriate amount of heme forms the basic elements of various life activities, while when released in large quantities, it ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2019.00825

    authors: Wu B,Wu Y,Tang W

    更新日期:2019-07-24 00:00:00

  • Chidamide Inhibits Acute Myeloid Leukemia Cell Proliferation by lncRNA VPS9D1-AS1 Downregulation via MEK/ERK Signaling Pathway.

    abstract::Irregular histone modification and aberrant lncRNAs expression are closely related to the occurrence of tumors including acute myeloid leukemia (AML). However, the effects and specific underlying molecular mechanism of histone deacetylase inhibitors on lncRNA expression in AML cells are unclear. Here, we reported the ...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2020.569651

    authors: Lin L,Que Y,Lu P,Li H,Xiao M,Zhu X,Li D

    更新日期:2020-10-19 00:00:00

  • Epidemiological associations between iron and cardiovascular disease and diabetes.

    abstract::Disruptions in iron homeostasis are linked to a broad spectrum of chronic conditions including cardiovascular, malignant, metabolic, and neurodegenerative disease. Evidence supporting this contention derives from a variety of analytical approaches, ranging from molecular to population-based studies. This review focuse...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2014.00117

    authors: Basuli D,Stevens RG,Torti FM,Torti SV

    更新日期:2014-05-20 00:00:00

  • MicroRNA-532-3p Suppresses Malignant Behaviors of Tongue Squamous Cell Carcinoma via Regulating CCR7.

    abstract::To provide better therapeutic avenues for treating tongue squamous cell carcinoma (TSCC), a series of experiments about the effects of microRNA (miR)-532-3p on TSCC malignant behaviors were carried out. The result showed that miR-532-3p was down-regulated and C-C chemokine receptor 7 (CCR7) was up-regulated in the tum...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2019.00940

    authors: Feng C,So HI,Yin S,Su X,Xu Q,Wang S,Duan W,Zhang E,Sun C,Xu Z

    更新日期:2019-08-29 00:00:00

  • Composition and Functionality of Lipid Emulsions in Parenteral Nutrition: Examining Evidence in Clinical Applications.

    abstract::Lipid emulsions (LEs), an integral component in parenteral nutrition (PN) feeding, have shifted from the primary aim of delivering non-protein calories and essential fatty acids to defined therapeutic outcomes such as reducing inflammation, and improving metabolic and clinical outcomes. Use of LEs in PN for surgical a...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章,评审

    doi:10.3389/fphar.2020.00506

    authors: Sadu Singh BK,Narayanan SS,Khor BH,Sahathevan S,Abdul Gafor AH,Fiaccadori E,Sundram K,Karupaiah T

    更新日期:2020-04-29 00:00:00

  • Tnfrsf12a-Mediated Atherosclerosis Signaling and Inflammatory Response as a Common Protection Mechanism of Shuxuening Injection Against Both Myocardial and Cerebral Ischemia-Reperfusion Injuries.

    abstract::Shuxuening injection (SXNI) is a widely prescribed herbal medicine of Ginkgo biloba extract (EGB) for cerebral and cardiovascular diseases in China. However, its curative effects on ischemic stroke and heart diseases and the underlying mechanisms remain unknown. Taking an integrated approach of RNA-seq and network pha...

    journal_title:Frontiers in pharmacology

    pub_type: 杂志文章

    doi:10.3389/fphar.2018.00312

    authors: Lyu M,Cui Y,Zhao T,Ning Z,Ren J,Jin X,Fan G,Zhu Y

    更新日期:2018-04-06 00:00:00