Abstract:
BACKGROUND:The major obstacle to cure of HIV type-1 infection is the presence of the HIV reservoir, hidden from the immune system and insensitive to combined antiretroviral therapy (cART). Eradication approaches have been hindered by the difficulty for accurately monitoring its size in vivo, especially in the lymphoid organs. Humanized mouse models are a valuable tool for systematically assess the efficacy of therapeutic interventions in reducing the HIV reservoir. Nonetheless, persistence of the HIV reservoir over time, in the presence of cART, has yet to be analyzed in this in vivo model. FINDINGS:We found that the proviral DNA as well as the total DNA were very stable in the spleen and mesenteric lymph node irrespective of the length of cART. Notably, the amount of proviral DNA was very similar in the spleen and lymph node. Furthermore, we observed a correlation between the percentage of splenic human CD4+ T-cells with total HIV DNA, between the number of human CD38 + CD8+ T-cells in the spleen with the amount of integrated HIV DNA, and eventually between the hCD4/hCD8 ratio in the spleen with integrated as well as total HIV DNA implying that the CD8+ T cells influence the size of the HIV reservoir. CONCLUSIONS:Here, we demonstrated the stability of this reservoir in humanized mice irrespective of the length of cART, confirming the relevancy of this model for HIV latency eradication investigations. Notably, we also found correlates between the frequency of CD4+ T-cells, their activation status and viral parameters, which were analogous to the ones in HIV-infected patients. Thus, hu-mice represent a very valuable HIV latency model.
journal_name
Virol Jjournal_title
Virology journalauthors
Rochat MA,Schlaepfer E,Kuster SP,Li D,Audige A,Ivic S,Fahrny A,Speck RFdoi
10.1186/s12985-018-1101-9subject
Has Abstractpub_date
2018-12-17 00:00:00pages
191issue
1issn
1743-422Xpii
10.1186/s12985-018-1101-9journal_volume
15pub_type
杂志文章相关文献
Virology Journal文献大全abstract:BACKGROUND:Respiratory syncytial virus (RSV), human Rhinovirus (HRV) and human Metapneumo Virus (HMPV) are important viral pathogens causing acute respiratory tract infections in the hospitalized patients. Sensitive and accurate detection of RSV, HRV and HMPV is necessary for clinical diagnosis and treatment. RESULTS:...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-1061-0
更新日期:2018-10-30 00:00:00
abstract::Hepatitis E virus (HEV) is a zoonotic pathogen of which several species of animal were reported as reservoirs. Swine stands out as the major reservoir for HEV infection in humans, as suggested by the close genetic relationship of swine and human virus and cross-species infection of HEV. Up to now, the mechanism of cro...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-482
更新日期:2011-10-27 00:00:00
abstract::The prevalence of North American orthopoxviruses in nature is unknown and may be more difficult to ascertain due to wide spread use of vaccinia virus recombinant vaccines in the wild. A real time PCR assay was developed to allow for highly sensitive and specific detection of North American orthopoxvirus DNA in animal ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-313
更新日期:2011-06-20 00:00:00
abstract:BACKGROUND:Genome-wide RNAi screening has been widely used to identify host proteins involved in replication and infection of different viruses, and numerous host factors are implicated in the replication cycles of these viruses, demonstrating the power of this approach. However, discrepancies on target identification ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0420-3
更新日期:2015-11-24 00:00:00
abstract:BACKGROUND:Human adenoviruses (Ads) have substantial potential for clinical applications in cancer patients. Conditionally replicating adenoviruses (CRAds) include oncolytic adenoviruses in which expression of the immediate early viral transactivator protein E1A is controlled by a cancer cell-selective promoter. To enh...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-175
更新日期:2010-07-29 00:00:00
abstract:BACKGROUND:Human pegivirus-1 (HPgV-1) is a member of the Flaviviridae family whose genomic organization and mode of cellular entry is similar to that of hepatitis C virus (HCV). The E2 glycoprotein of HPgV-1 is the principle mediator in the virus-cell interaction and as such harbors most of HPgV-1's antigenic determina...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0837-y
更新日期:2017-08-31 00:00:00
abstract::Recombination is known to play a role in the ability of various viruses to acquire sequence diversity. We consequently examined all available West Nile virus (WNV) whole genome sequences both phylogenetically and with a variety of computational recombination detection algorithms. We found that the number of distinct l...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-165
更新日期:2009-10-12 00:00:00
abstract:BACKGROUND:The plateau pika (Ochotona curzoniae) is a small rabbit-like mammal that lives at high altitudes in the Qinghai-Tibet plateau and is in close contact with birds. Following the outbreak of highly pathogenic avian influenza (HPAI) H5N1 during 2005 in the migratory birds of Qinghai Lake, two clades of H5N1 have...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0290-8
更新日期:2015-04-14 00:00:00
abstract:BACKGROUND:Public health risks associated to infection by human coronaviruses remain considerable and vaccination is a key option for preventing the resurgence of severe acute respiratory syndrome coronavirus (SARS-CoV). We have previously reported that antibodies elicited by a SARS-CoV vaccine candidate based on recom...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-82
更新日期:2014-05-06 00:00:00
abstract:BACKGROUND:The human polyomavirus BK expresses a 66 amino-acid peptide referred to as agnoprotein. Though mutants lacking agnoprotein are severely reduced in producing infectious virions, the exact function of this peptide remains incompletely understood. To elucidate the function of agnoprotein, we searched for novel ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-014-0220-1
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND:The diseases caused by Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) have been occurring epidemically in China and southeastern Asia in recent years. A sensitive, reliable and quantitative method is required to detect and distinguish for RBSDV and SRBSDV in ric...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-24
更新日期:2013-01-18 00:00:00
abstract:BACKGROUND:The threat of recurring influenza pandemics caused by new viral strains and the occurrence of escape mutants necessitate the search for potent therapeutic targets. The dependence of viruses on cellular factors provides a weak-spot in the viral multiplication strategy and a means to interfere with viral multi...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-108
更新日期:2010-05-26 00:00:00
abstract:BACKGROUND:Saffold Virus (SAFV) is a human cardiovirus that is suspected of causing infection of the central nervous system (CNS) in children. While recent animal studies have started to elucidate the pathogenesis of SAFV, very little is known about the mechanisms behind it. METHOD:In this study, we attempted to eluci...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0654-8
更新日期:2016-11-25 00:00:00
abstract::Hairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However, it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses, the major route of plant virus spread in nature. As a proof-of-principle strategy, w...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-42
更新日期:2008-03-19 00:00:00
abstract::In order to improve the presentation and immunogenicity of single epitopes, virus-like particles (VLPs) are being used as platforms for the display of foreing epitopes on their surface. The rotavirus major capsid protein VP6 has the ability to self-assemble into empty non-infectious VLPs. In the present study, we anal...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-192
更新日期:2009-11-06 00:00:00
abstract:BACKGROUND:A number of gene therapy applications would benefit from vectors capable of expressing multiple genes. In this study we explored the feasibility and efficiency of expressing two or three transgenes in HIV-1 based lentiviral vector. Bicistronic and tricistronic self-inactivating lentiviral vectors were constr...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-3-14
更新日期:2006-03-15 00:00:00
abstract:BACKGROUND:West Nile virus (WNV) and Usutu virus (USUV), both belonging to the genus Flavivirus, are emerging in Italy as important human and animal pathogens. Migratory birds are involved in the spread of Flaviviruses over long distances, particularly from Africa to Europe. Once introduced, these viruses can be furthe...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-306
更新日期:2013-10-11 00:00:00
abstract:BACKGROUND:Avian hepatitis E virus (aHEV) has been associated with hepatitis-splenomegaly syndrome (HSS) in chickens along with asymptomatic subclinical infection in many cases. So far, four genotypes have been described, which cause infection in chickens, specifically in broiler breeders and layer chickens. In the pre...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-019-1247-0
更新日期:2019-11-21 00:00:00
abstract::During the outbreak of SARS in 2002/3, a prototype virus was isolated from a patient in Frankfurt/Germany (strain Frankfurt-1). As opposed to all other SARS-Coronavirus strains, Frankfurt-1 has a 45-nucleotide deletion in the transmembrane domain of its ORF 7b protein. When over-expressed in HEK 293 cells, the full-le...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-131
更新日期:2009-08-24 00:00:00
abstract::HIV expressed in genital secretions provides the inoculum from which transmitting variants are selected, both in sexual transmission and mother-to-infant transmission during partuition. Characterization of HIV levels and genotypes found in vaginal secretions and the impact of anti-retroviral therapy (ART) on this viru...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-87
更新日期:2008-07-29 00:00:00
abstract:BACKGROUND:Recent studies have revealed that Mitochondrial Antiviral Signaling (MAVS) protein plays an essential role in the inhibition of viral infection through type I interferon (IFN) pathway. It has been shown that 3C (pro) cysteine protease of coxsackievirus B3 (CVB3) cleaves MAVS to inhibit type I IFNs induction....
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-312
更新日期:2012-12-19 00:00:00
abstract:BACKGROUND:Post-transplant lymphoproliferative disorders (PTLD) are serious complications in lung transplant recipients. No consensus on EBV DNAemia levels predictive of PTLD has been reached. In addition, in many instances EBV DNAemia is determined in patients with suggestive symptoms only. METHODS:The characteristic...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-421
更新日期:2011-09-05 00:00:00
abstract:BACKGROUND:The deciphering of cellular networks to determine susceptibility to infection by HIV or the related simian immunodeficiency virus (SIV) is a major challenge in infection biology. RESULTS:Here, we have compared gene expression profiles of a human CD4+ T cell line at 24 h after infection with a cell line of t...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-152
更新日期:2014-08-27 00:00:00
abstract::Measles, caused by measles virus (MeV), is a common infection in children. MeV is a member of the genus Morbillivirus and is most closely related to rinderpest virus (RPV), which is a pathogen of cattle. MeV is thought to have evolved in an environment where cattle and humans lived in close proximity. Understanding th...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-52
更新日期:2010-03-04 00:00:00
abstract:BACKGROUND:Zika virus (ZIKV) is a major human pathogen and member of the Flavivirus genus. Previous studies have identified neutralizing antibodies from Zika patients that bind to quaternary epitopes across neighboring envelope (E) proteins, called E dimer epitopes (EDE). An asparagine-linked glycan on the "glycan loop...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-020-01313-1
更新日期:2020-03-31 00:00:00
abstract:BACKGROUND:The family Mesoniviridae (order Nidovirales) comprises of a group of positive-sense, single-stranded RNA ([+]ssRNA) viruses isolated from mosquitoes. FINDINGS:Thirteen novel insect-specific virus isolates were obtained from mosquitoes collected in Indonesia, Thailand and the USA. By electron microscopy, the...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-97
更新日期:2014-05-20 00:00:00
abstract:BACKGROUND:Aleutian mink disease has major economic consequences on the mink farming industry worldwide, as it causes a disease that affects both the fur quality and the health and welfare of the mink. The virus causing this disease is a single-stranded DNA virus of the genus Amdoparvovirus belonging to the family of P...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0898-y
更新日期:2017-11-22 00:00:00
abstract:OBJECTIVE:To determine HIV-1 RNA load during the third trimester of pregnancy and evaluate its effect on in utero and intra-partum/postpartum transmissions in a breastfeeding population. DESIGN:A nested case-control study within a PMTCT cohort of antiretroviral therapy naive pregnant women and their infants. METHODS:...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-176
更新日期:2010-08-02 00:00:00
abstract:BACKGROUND:The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria. METHODS:Staphylococcus phage JD007 was characterized and its complete genome sequence analysed. RESULTS:Staphylococcus phage JD007 was classified as belonging to the Myoviridae family based on ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0701-0
更新日期:2017-02-08 00:00:00
abstract:BACKGROUND:The binding of viral-specific antibodies to cell-surface antigens usually results in down modulation of the antigen through redistribution of antigens into patches that subsequently may be internalized by endocytosis or may form caps that can be expelled to the extracellular space. Here, by use of confocal-l...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-68
更新日期:2007-07-03 00:00:00