Abstract:
:BACKGROUND Hypoxia induces cell apoptosis in the uterosacral ligaments of patients with pelvic organ prolapse by upregulation of hypoxia-inducible factor-1α (HIF-1α). This study aimed to investigate the effects of HIF-1α on human uterosacral ligament fibroblasts (hUSLFs) following treatment with the chemical inducer of hypoxia, cobalt chloride (CoCl2), and to explore the underlying mechanisms. MATERIAL AND METHODS Ten women who underwent hysterectomy for benign disease provided uterosacral ligament tissue for cell extraction. Following CoCl₂ treatment, cell viability of isolated and cultured hUSLFs was evaluated by the MTT assay. JC-1 fluorescence mitochondrial imaging was used to study the change in mitochondrial membrane potential. Cell apoptosis and expression of apoptosis-associated proteins and collagen type I alpha 1 (COL1A1) were measured by flow cytometry, TUNEL and Western blot, respectively. RESULTS Hypoxia increased the expression of HIF-1a and increased cell apoptosis, decreased cell viability and expression levels of COL1A1. The JC-1 assay showed that the mitochondrial membrane potential was reduced and caspase-8, and -9 inhibitors partly reduced hUSLF apoptosis. HIF-1α treatment downregulated the expression of cellular FLICE inhibitory protein (c-FLIP), decoy receptor 2 (DcR2), and the ratio of Bcl-2 to Bax, and upregulated the expression tumor necrosis factor related apoptosis-inducing ligand (TRAIL), death receptor 5 (DR5) or TRAIL-R2, Fas, Bcl-2 interacting protein 3 (BNIP3), and cytochrome C, and increased the activation of caspase-3, caspase-8, and caspase-9, all of which were reversed by knockdown of HIF-1α. CONCLUSIONS HIF-1α significantly induced apoptosis of hUSLFs through both the cell death receptor and the mitochondrial-associated apoptosis pathways.
journal_name
Med Sci Monitjournal_title
Medical science monitor : international medical journal of experimental and clinical researchauthors
Zhao X,Liu L,Li R,Wei X,Luan W,Liu P,Zhao Jdoi
10.12659/MSM.913384subject
Has Abstractpub_date
2018-12-03 00:00:00pages
8722-8733eissn
1234-1010issn
1643-3750pii
913384journal_volume
24pub_type
杂志文章abstract:BACKGROUND:Currently available treatment options for decompensated hepatitis B-induced liver cirrhosis are limited and largely ineffective. Recently, stem cell transplantation has emerged as a promising treatment for cirrhosis. The aim of this study was to determine whether autologous peripheral blood stem cell transpl...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.892990
更新日期:2015-05-13 00:00:00
abstract::BACKGROUND miR-23b overexpression can promote cardiomyocyte apoptosis and reduce cell growth under hypoxic conditions, suggesting that miR-23b acts as a biomarker for ST-elevation myocardial infarction (STEMI). The aim of this study was to investigate the effect of miR-23b on STEMI patients. MATERIAL AND METHODS We en...
journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.908060
更新日期:2018-03-14 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/MSM.912624
更新日期:2019-01-11 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:10.12659/msm.883271
更新日期:2012-08-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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更新日期:2018-04-15 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2009-08-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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更新日期:2018-12-06 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,评审
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更新日期:2000-01-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章,meta分析
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更新日期:2015-02-27 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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doi:10.12659/MSM.923552
更新日期:2020-07-21 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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doi:
更新日期:2010-04-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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更新日期:2020-08-08 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
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更新日期:2020-03-23 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 杂志文章
doi:
更新日期:2010-12-01 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
pub_type: 临床试验,杂志文章
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更新日期:2019-09-23 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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更新日期:2014-01-10 00:00:00
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journal_title:Medical science monitor : international medical journal of experimental and clinical research
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