Abstract：
:The miR-200 family suppresses epithelial-mesenchymal transition by inhibiting ZEB1 and ZEB2 mRNA translation in several types of cancers. Kindlin-2 is a target gene of miR-200b and its expression level correlates positively to ZEB2 in oral squamous cell carcinoma (OSCC). Whether Kindlin-2 and ZEB2 share a competitive endogenous RNAs regulatory network in OSCC remains unclear. Here, we studied the expression levels of miR-200b, Kindlin-2, and ZEB2 and found direct interaction between miR-200b, ZEB2, and Kindlin-2 mRNA in OSCC. A series of experiments was performed to elucidate the role of miR-200b and Kindlin-2 in OSCC cells. To further investigate whether Kindlin-2 regulates ZEB2 as a "ceRNA", we utilized pools of siRNAs to deplete Kindlin-2 or ZEB2 in Tca-8113 cells. Significantly elevated expression levels of Kindlin-2 and ZEB2, down-regulated mRNA levels of miR-200b, and a positive correlation between Kindlin-2 and ZEB2 were found in OSCC cells. Additional results suggest that miR-200b directly targets ZEB2 and that Kindlin-2 3'UTR miR-200b repressed both the migration and invasive functionality of Tca-8113. Kindlin-2 and ZEB2 are involved in accelerated migration and invasion of Tca-113 cells in vitro and Kindlin-2 controlled ZEB2 expression. However, Kindlin-2-mediated ZEB2 regulation did not depend on miRNAs. These results indicate that Kindlin-2 does not act as ZEB2 ceRNA and modify the migration of Tca-8113 cells. Our results improve our understanding of the underlying molecular and cellular mechanisms of oral cancer metastasis.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Ren W,Gao L,Qiang C,Li S,Zheng J,Wang Q,Zhi Y,Cai G,Kong X,Zhou M,Qu Z,Zhi Ksubject
Has Abstractpub_date
2018-08-15 00:00:00pages
2529-2541issue
8issn
1943-8141journal_volume
10pub_type
杂志文章abstract：BACKGROUND:This study tested the long-term effect of extracorporeal shock wave (ECSW) therapy on ameliorating radiotherapy-induced chronic cystitis (CC) in rat. METHODS AND RESULTS:Adult-female SD rats (n = 24) were equally categorized into group 1 (normal control), group 2 (CC induced by radiotherapy with 450 cGy twi...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-03-15 00:00:00
abstract：BACKGROUND:Cisplatin is a common used anti-tumor drug in ovarian cancer therapy with potent effect. Studies have reported that autophagy works as a cell-survival process in cancer, chloroquine has been added to various chemotherapeutic drugs. In the current study, we aim to evaluate whether chloroquine can enhance the ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-09-15 00:00:00
abstract：BACKGROUND:To further explore the role of PPARγ in QL treatment, ISO-induced mice model and following methods were established. METHODS:Cardiac remodeling on mice model was induced by isoproterenol (ISO) infusion or saline infusion as control for two weeks then divided into 4 groups, after that divided in 5 different ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-12-15 00:00:00
abstract：:Objectives: Persistent low-grade chronic inflammation is common in type 2 diabetes (T2D) and obesity. To date, the underlying molecular mechanism is not well understood. In this study, we aimed to investigate gut microbiota and the expression of monocyte surface molecules in obese T2D subjects who underwent Roux-en-Y ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-03-15 00:00:00
abstract：:The skin serves as a physical and chemical barrier to provide an initial line of defense against environmental threats; however, this function is impaired in diabetes. Vγ4 γ δ T cells in the dermis are an important part of the resident cutaneous immunosurveillance program, but these cells have yet to be explored in th...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-06-15 00:00:00
abstract：:A keloid is the process of skin healing, collagen synthesis and metabolism of the loss of normal control in a sustained hyperactive state, resulting in excessive proliferation of collagen fibers. A large-scale genome-wide association study (GWAS) has identified multiple single nucleotide polymorphisms (SNPs) in the 3q...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-02-15 00:00:00
abstract：:MicroRNAs (miRNAs) are involved in the development and progression of lung cancer. MicroRNA-101 (miR-101) displays crucial properties in non-small cell lung cancer (NSCLC) by negatively regulating cell proliferation and invasion, but the underlying molecular mechanisms remain largely unknown. In this study, we found t...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-04-15 00:00:00
abstract：:HNF1A-antisense 1 (HNF1A-AS1), a long non-coding RNA (lncRNA), is associated with metastasis and is an independent prognostic factor for lung cancer. Recent studies demonstrated that HNF1A-AS1 play important roles in cacinogenesis. However, the exact effects and molecular mechanisms of HNF1A-AS1 in osteosarcoma (OS) p...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-08-15 00:00:00
abstract：:Cyclooxygenase-2 expression by malignant tumors, including colonic adenocarcinoma, is associated with increased tumor aggression and poor prognosis. Nuclear factor kappa B is a key regulator of cyclooxygenase-2 and is regulated by two pathways, the 'canonical' and the 'alternative' pathway. The alternative pathway is ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2015-09-15 00:00:00
abstract：BACKGROUND:Cyclosporin A (CsA) and tacrolimus block T cell activation by inhibiting the phosphatase calcineurin and preventing translocation from the cytoplasm to the nucleus of the transcription factor Nuclear Factor of Activated T cells (NFAT). NFAT compose a family of transcription factors that are turned on during ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2009-01-22 00:00:00
abstract：:Biceps tendinopathy is a relatively common ailment that typically presents as pain, tenderness, and weakness in the tendon of the long head of the biceps brachii. Though it is often associated with degenerative processes of the rotator cuff and the joint, this is not always the case, thus, the etiology remains conside...
journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2017-06-15 00:00:00
abstract：OBJECTIVES:Whether polycystic ovary syndrome (PCOS) is associated with mtDNA or nDNA mutations causing mitochondrial disorders (MIDs) or not is under debate. This review aims at summarising and discussing previous and recent findings concerning the frequency, diagnosis, and treatment of PCOS in MIDs. METHODS:Systemati...
journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2018-01-15 00:00:00
abstract：:Random pattern skin flap transplantation is frequently applied in plastic and reconstructive surgery, but the distal part of skin flaps often suffers necrosis due to ischemia. Astragaloside IV (AS-IV), a natural saponin purified from Astragalus membranaceus, may have beneficial functions for flap survival. In this stu...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-09-15 00:00:00
abstract：:Angiotesin II (Ang II) plays an important role in cardiac remodeling. Fibroblast growth factor inducible-14 (Fn14) is the smallest member of the tumor necrosis factor superfamily of receptors. Currently, little is known about the functional role of Fn14 in the heart. Chiefly, we observe the up-regulation of extracellu...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-12-15 00:00:00
abstract：BACKGROUND:Gastric cancer (GC) is one of the most common digestive tract tumors, and a serious threat to human health. Long non-coding RNA (lncRNA) are involved in many cancers. However, the biological functions of most lncRNAs are unclear. In this study, we investigated the mechanisms by which FLVCR1-AS1 regulated GC ...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-02-15 00:00:00
abstract：OBJECTIVE:Comparative study of type 2 diabetes and healthy controls by metabolomics methods to explore the pathogenesis of Type II diabetes. METHODS:Gas chromatography - mass spectrometry (GC-MS) with a variety of multivariate statistical analysis methods to the healthy control group 58 cases, 68 cases of Type II diab...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-07-15 00:00:00
abstract：:The aim of this study was to evaluate the effects of miR-1180 on pancreatic cancer. We sampled adjacent cancer and carcinoma tissues from 30 pancreatic cancer patients and measured miR-1180 expression by qRT-PCR and NF-κB protein expression by immunohistochemistry. To explore the effects of this miRNA in cell culture,...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-06-15 00:00:00
abstract：:Macrophages participate in all stages of sepsis and affect immune homeostasis and inflammatory processes. Small ubiquitin-like modifier (SUMO) protease SENP1 plays an important role in cellular inflammation by regulating proteins in SUMOylation. However, the roles and related mechanisms of SENP1 in macrophage inflamma...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-11-15 00:00:00
abstract：:Mast cells are important effector cells of the immune system, and mast cell-derived exosomes carrying RNAs play a role in immune regulation. However, the molecular function of mast cell-derived exosomes is currently unknown, and here, we identify differentially expressed genes (DEGs) in mast cells and exosomes. We iso...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-02-15 00:00:00
abstract：:Severe air pollution has raised concerns about the adverse effects of particulate matters 2.5 μm in size (PM2.5) on human health. However, the mechanisms elucidating how PM2.5 affects lungs, especially in COPD, remain unclear. In this study, we examined the concentration changes of environmental PM2.5 from 2013 to 201...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2020-09-15 00:00:00
abstract：:Cullin-5 (CUL5), a scaffold protein in active cullin-RING ubiquitin ligase (CRL) complexes, is a member of the cullin family of proteins. The CUL5-type ubiquitin ligase can target multiple proteins involved in ubiquitination and proteasome degradation. CUL5 plays positive roles in regulating cell growth, proliferation...
journal_title：American journal of translational research
pub_type： 杂志文章,评审
doi：
更新日期：2020-02-15 00:00:00
abstract：:Babaodan capsule (BBD), a traditional Chinese (TCM) formula, has been widely used as an alternative remedy for multiple types of malignancies, clinically. However, the underlying mechanisms behind the efficacy of BBD remain poorly understood, particularly in regard to lung cancer. Herein, we demonstrate that BBD induc...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-08-15 00:00:00
abstract：:Circular RNA hsa_circ_0002024 has been reported to be underexpressed in bladder cancer (BC). However, the biological role of hsa_circ_0002024 in BC and its underlying molecular mechanisms remain unclear. In this study, the expression levels of hsa_circ_0002024 and miR-197-3p were examined by quantitative real-time pol...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-03-15 00:00:00
abstract：BACKGROUND:Recurrent hepatocellular carcinoma (rHCC) patients with microvascular invasive (MVI) positive at first resection usually had poorly differentiated tumors and worse survivals. The optimal treatment for this population remains to be elucidated. METHODS:We retrospectively analyzed 319 rHCC patients with MVI-po...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2019-03-15 00:00:00
abstract：:Interleukin-21 (IL-21) and T helper 17 (Th17) cells are known to be involved in the pathogenesis of psoriasis, but little is known about their relationship in psoriasis. Herein, we investigated whether IL-21 could regulate Th17 cell induction in patients with psoriasis vulgaris. 32 patients with psoriasis vulgaris and...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-07-15 00:00:00
abstract：BACKGROUND:Glioma is the most common malignant tumor in the adult human brain and has one of the lowest patient survival rates. MicroRNAs (miRNAs) play important roles in the development of cancers, including glioma, and potentially have valuable therapeutic applications in glioma; however, their specific functions and...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2018-09-15 00:00:00
abstract：:Long non-coding RNAs (lncRNAs) have been recently reported to be dysregulated and play a critical role in the progression of thyroid cancer. Here, we found that the lncRNA n340790 was highly expressed in human thyroid cancer tissues and was strongly correlated with the clinical characteristics of patients. There was a...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2017-05-15 00:00:00
abstract：:Interleukin-1β (IL-1β) plays an important role in brain injury after focal ischemia, and bone marrow-derived mesenchymal stem cells (BMSCs) are capable of reducing the expression of IL-1β, we investigated the effects of BMSCs transplantation on brain edema and cerebral infarction as well as the underlying mechanisms v...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2016-03-15 00:00:00
abstract：:CD105 (endoglin) is an independent marker for poor prognosis in more than 10 solid tumor types. The goal of this study was to develop a CD105-specific agent for both positron emission tomography (PET) and near-infrared fluorescence (NIRF) imaging, which has potential clinical applications in the diagnosis and imaged-g...
journal_title：American journal of translational research
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Chronic myelomonocytic leukemia (CMML) can be complicated by autoimmune features associated with rheumatologic disorders, which have been commonly reported by more researches currently. The intrinsic correlation between CMML and autoimmune diseases can create significant pitfalls in differential diagnosis. CMML occasi...
journal_title：American journal of translational research
pub_type：
doi：
更新日期：2019-07-15 00:00:00
