Abstract:
BACKGROUND:Circular RNAs (circRNAs) represent a class of non-coding RNAs that are emerging as important regulators during tumorigenesis and provide potential targets for cancer intervention. However, the expression profiles and functions of circRNAs in hepatocellular carcinoma (HCC) have not been completely clarified. Herein, the role of hsa_circ_0103809 was investigated in HCC tissues and cell lines. METHODS:High-throughput circRNA sequencing was performed to detect the expression profiles of circRNA in HCC tissues. The CCK-8, wound healing and flow cytometry were performed to measure the cell viability, migration and apoptosis in HCC cells. The expression levels of gene and protein in HCC tissues and cell lines were assayed by RT-qPCR and western blotting, respectively. Immunohistochemical staining was used to assess the protein expression of SOX2 in HCC tissues. RESULTS:We discovered that hsa_circ_0103809 was significantly increased in HCC tissues and cell lines. Knockdown of hsa_circ_0103809 inhibited proliferation and migration and induced apoptosis in HCC cell lines. Investigation to the molecular mechanisms of hsa_circ_0103809 in HCC cells had revealed that hsa_circ_0103809 directly suppressed miR-490-5p, which targeted to the 3'-UTR of SOX2. Hsa_circ_0103809 loss-of-function could increase the expression of miR-490-5p as well as decreased the expression of SOX2. Furthermore, we found that si-0103809 induced growth and migration inhibition and apoptosis could be reversed by transfected with miR-490-5p inhibitors or SOX2 in HCC cells. CONCLUSION:Our findings suggested that hsa_circ_0103809 might facilitate HCC malignant progression, at least partially, by regulating miR-490-5p/SOX2 signaling pathway.
journal_name
Am J Transl Resjournal_title
American journal of translational researchauthors
Cai H,Hu B,Ji L,Ruan X,Zheng Zsubject
Has Abstractpub_date
2018-06-15 00:00:00pages
1690-1702issue
6issn
1943-8141journal_volume
10pub_type
杂志文章abstract:BACKGROUNDS:The deubiquitinating enzyme OTUB1 participates in multiple cellular processes. However, its expression and functions in gastric adenocarcinoma remains unknown. The aim of this study was to investigate the expression of OTUB1 and its biological role in gastric adenocarcinoma. METHODS:We used immunohistochem...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-05-15 00:00:00
abstract::We investigated the protective effects exerted by oridonin, the main active constituent of the Chinese medicinal herb Rabdosiarubescens, against lipopolysaccharide (LPS)/D-galactosamine (D-Gal)-induced acute liver injury (ALI). An ALI model was induced in mice using LPS (40 μg/0.5 ml) and D-Gal (5 mg/0.5 ml). The mice...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-09-15 00:00:00
abstract:OBJECTIVE:To create the rabbit animal model of intervertebral disc (IVD) degeneration by the annulus needle puncture technique through a novel transabdominal approach. METHODS:Thirteen New Zealand White rabbits underwent annular puncture at the L3/4, L4/5, and L5/6 discs through a transabdominal approach. For a longit...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-03-15 00:00:00
abstract:BACKGROUND:Chondrogenic differentiation of mesenchymal stem cells (MSCs) is important for osteoarthritis (OA) treatment. However, the specific mechanisms involved are undefined. MicroRNAs (miRNAs) downregulate protein synthesis by binding to the 3'UTR of target mRNA. METHODS:Bone marrow aspirates were obtained from OA...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-01-15 00:00:00
abstract::This study aimed to explore the effects of long non-coding RNA (lncRNA) expression on rheumatoid arthritis (RA). LncRNA expression profiles were obtained from the synovial tissues of five RA patients and five age-/gender-matched controls by RNA-Seq. Six candidate lncRNAs were then chosen and their levels in synovial f...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-04-15 00:00:00
abstract::Acquired resistance to chemotherapy is a major limitation for the successful treatment of lung cancer. Previously, we and others showed that formation of tumor spheres is associated with chemotherapy resistance in lung cancer cells, but the underlying mechanisms remained largely unknown. In the current study, we show ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-09-15 00:00:00
abstract::Liver cancer, one of the most common malignant tumors occurred worldwide, has emerged as a main health trouble and accounts for leading cancer-related death. Diosgenin is provided as an important material in the pharmaceutical industry, and is used to manage various medical troubles such as cancer because of its multi...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-11-15 00:00:00
abstract::An ideal implantation of artificial cervical disc replacement (ACDR) prosthesis should preserve the cervical biomechanics and maintain normal cervical biomechanics. In this study, we designed a novel prosthesis based on the physiological curvature of the cervical endplate, and conducted an in vitro test with cadaveric...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-05-15 00:00:00
abstract::Background: Pericardial patches are frequently used in vascular surgery to close arteriotomies. The early healing of these patches is mediated by attraction of CD34 and ephrinB2-positive cells. Atorvastatin is a commonly used statin drug that promotes healing of cardiovascular injury. We hypothesized that atorvastatin...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-12-15 00:00:00
abstract::Programmed cell death ligand 1 (PD-L1) is a key suppressor of the cytotoxic immune response. In colorectal carcinoma (CRC), PD-L1 expression results in immune escape and poor prognosis. Extensive researches suggested that metformin had a potential efficacy of enhancing anti-tumor immune response in different types of ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-11-15 00:00:00
abstract::Targeted oncolytic adenoviruses can selectively replicate in cancer cells; combined with traditional chemotherapy drugs, this approach is expected to become an important treatment method for overcoming the current bottleneck of osteosarcoma treatment. Here, we investigate the effect of oncolytic adenovirus Ad11 combin...
journal_title:American journal of translational research
pub_type: 杂志文章,收录出版
doi:
更新日期:2020-01-15 00:00:00
abstract::Aspergillus is widely distributed in the Earth's biosphere. It has strong adaptive capacity, and lives as saprophytic or parasitic life. This study aims to investigate the role of E-cadherin for adhesion of Aspergillus fumigatus blastospores in a human epithelial cell line (A549) and search the correlated molecule in ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-05-15 00:00:00
abstract::MicroRNAs (miRNAs) are small regulatory non-coding RNAs that have been reported to play an important role in the tumorigenesis of many cancers. In addition, miRNAs might serve as new promising biomarkers for diagnosis and prognosis and as effective therapeutic targets for patients with such malignancies. Accordingly, ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-03-15 00:00:00
abstract::The high-grade glioma (HGG) remains as the greatest challenge for cancer management worldwide. Identification of novel therapeutics and diagnostic method is in urgent need. The V-set and immunoglobulin domain-containing protein 4 (VSIG4) is a complement receptor for C3b/iC3b and inhibits cytotoxic T lymphocytes activa...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2015-06-15 00:00:00
abstract::Human noroviruses (HuNoVs) are a major cause of epidemic and sporadic cases of acute gastroenteritis worldwide. Recently, human intestinal enteroids (HIEs) have been shown to support the replication of HuNoVs, and be an excellent model to study HuNoV-host interactions. We implemented the HIE system in our laboratory a...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-06-15 00:00:00
abstract::The present study aimed to investigate the effect of genetic polymorphisms of catechol-O-methyl transferase (COMT), apolipoprotein E (APOE), and brain derived neurotrophic factor (BDNF) on the modulation of the chemotherapy-induced cognitive impairment (CICI) in breast cancer patients. Eighty triple negative breast ca...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-11-15 00:00:00
abstract::The objective of this paper was to investigate the suitability of the miniature pig as a large animal model of cochlear implantation (CI). Micro-CT scanning and three-dimensional reconstructions of the inner ear were completed in six animals. Photographs of the procedures and measurements of the inner ear were made. T...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-12-15 00:00:00
abstract::Anthracycline-based chemotherapy is a conventional treatment for breast cancer. However, it can negatively affect host immune function and thereby impair patients' quality of life. Boosting the host immune system and reducing the adverse effect of chemotherapy are important for effective cancer treatment. Natural kill...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-02-15 00:00:00
abstract:AIMS:This study was to investigate the effect of TUG1 on apoptosis and ECM degradation of human degenerative intervertebral disc nucleus pulposus cells (NPCs) and its mechanism. METHODS:Human degenerative intervertebral disc NP tissues were obtained from 10 patients with lumbar disc herniation (LDH) who underwent lumb...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-09-15 00:00:00
abstract::As a dual-acting neurotransmitter, glycine plays critical roles in cerebral ischemia by activating both glycine receptors (GlyRs) and N-methyl-D-aspartate acid receptors (NMDARs). However, the involvement of glycine receptor alpha 2 (GlyRa2) in cerebral ischemia has not been explored. The objective of this study was t...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-10-15 00:00:00
abstract::Myoblasts characterize by the potential to transform into skeletal muscle, and involve in processes of proliferation, differentiation and apoptosis. Mammalian target of rapamycin (mTOR) is an important protein of PI3K signaling pathway in muscle metabolism and physiology. This study aimed to investigate effects of mTO...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-12-15 00:00:00
abstract:AIM:Geniposide is an iridoid glycoside isolated from the gardenia plant. It has multiple biological activities. The roles of geniposide in systemic sclerosis (SSc) and in endothelial-to-mesenchymal transition (EndMT) are unclear. We investigated the protective effects of geniposide in a bleomycin-induced SSc mouse mode...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-03-15 00:00:00
abstract::The heart is an organ with extremely high energy expenditure, and cardiac performance is consistent with its metabolic level. Under pathological situations, the heart adjusts its metabolic pattern through mitochondrial regulation and substrate selection to maintain energy homeostasis. Heart failure is associated with ...
journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2020-10-15 00:00:00
abstract::Coronavirus disease 2019 (COVID-19) infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic disease with high morbidity and mortality. Inflammatory and thrombosis are its main manifestations. As an important organ of hemofiltration metabolism, the kidney is prone to blocka...
journal_title:American journal of translational research
pub_type: 杂志文章,评审
doi:
更新日期:2020-12-15 00:00:00
abstract:BACKGROUND:Nasopharyngeal carcinoma (NPC) is a kind of head-neck malignant neoplasm originated from the nasopharyngeal epithelium and is mainly prevalent in Southern China and Southeast Asia countries. KiSS-1 is an inhibitor of tumor metastasis in a range of cancers. METHODS:We establish a cell substrain of SUNE-1-5-8...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2019-02-15 00:00:00
abstract::Spinal cord injury (SCI) can lead to different levels of paraplegia. Studies have shown that exercise exerts wide protective effects against various diseases, and microRNAs (miRNAs) are involved in its beneficial effects. However, the specific role of miRNAs in the protective effects of exercise against SCI remains un...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2020-09-15 00:00:00
abstract::MicroRNAs (miRNAs) have been involved in some human malignancies and correlated with tumor progression. The dysregulation of miR-130 is found in various cancers and correlated with tumor proliferation and apoptosis. However, its expression and function in non-small cell lung cancer (NSCLC) have not been investigated y...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2017-04-15 00:00:00
abstract:BACKGROUND:Glioma is the most common malignant tumor in the adult human brain and has one of the lowest patient survival rates. MicroRNAs (miRNAs) play important roles in the development of cancers, including glioma, and potentially have valuable therapeutic applications in glioma; however, their specific functions and...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2018-09-15 00:00:00
abstract::Consumption of cruciferous vegetables is associated with a decreased risk of developing prostate cancer. Antineoplastic effects of cruciferous vegetables are attributable to bioactive indoles, most prominently, 3, 3'-diindolylmethane (DIM). In addition to effects on proliferation and apoptosis, DIM acts as an antiandr...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-01-15 00:00:00
abstract::Pancreatic cancer ranks No.1 in mortality rate worldwide. This study aims to identify the novel anti-pancreatic cancer drugs. Human pancreatic carcinoma cell lines were purchased from ATCC. CPE-based screening assay was used to examine the cell viability. Patient derived tumor xenografts in SCID mice was established. ...
journal_title:American journal of translational research
pub_type: 杂志文章
doi:
更新日期:2016-02-15 00:00:00